Review
Chemistry, Multidisciplinary
Alessandra Dellaquila, Chau Le Bao, Didier Letourneur, Teresa Simon-Yarza
Summary: The study discusses the importance of vascularization techniques in tissue engineering and the current research progress. By using different strategies and materials, more biologically relevant and organ-specific 3D models can be established, which are expected to be used for regenerative medicine and drug development in the future.
Review
Biochemistry & Molecular Biology
Flavia Carton, Manuela Malatesta
Summary: Nanoconstructs developed for biomedical purposes need to overcome biological barriers. In vivo models are complex and limited, so simplified in vitro models are preferred. Various in vitro models have been set up, including 2D cell cultures, cell monolayers, 3D cell cultures, and bioreactors. This review provides an overview of the most representative and performing in vitro models for studying biological barriers in nanomedical research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Terrence T. Roh, Ying Chen, Sara Rudolph, Michelle Gee, David L. Kaplan
Summary: There are inherent differences between human and animal systems, thus the development of in vitro models is crucial for understanding the intestinal innate immune system.
TRENDS IN BIOTECHNOLOGY
(2021)
Review
Crystallography
Ana Petrovic, Tomislav Kizivat, Ines Bilic Curcic, Robert Smolic, Martina Smolic
Summary: Urolithiasis is a multifactorial disease with a high recurrence rate, characterized by solid deposits forming in the urinary tract. The most common type is calcium oxalate stones. In vitro models offer insight into molecular processes leading to renal stone formation and are significant for patient management.
Review
Biochemistry & Molecular Biology
Shi-Xiang Wang, Ji-Song Yan, Yun-Shen Chan
Summary: This review discusses the use of human cell and organoid models for disease modeling in MAFLD. These models can be used to study the mechanisms of disease development and evaluate human-specific drug responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Camille Jubelin, Javier Munoz-Garcia, Laurent Griscom, Denis Cochonneau, Emilie Ollivier, Marie-Francoise Heymann, Francois M. Vallette, Lisa Oliver, Dominique Heymann
Summary: Cancer is a complex disease that requires multidimensional models to understand its development and drug response. Three-dimensional cell culture models, compared to traditional two-dimensional cultures, better mimic the biological behavior of tumor cells and provide a better platform for studying therapeutic escape and drug resistance.
CELL AND BIOSCIENCE
(2022)
Article
Immunology
Tomonori Takano, Hayami Kudo, Shuhei Eguchi, Asami Matsumoto, Kentaro Oka, Yukitaka Yamasaki, Motomichi Takahashi, Takuro Koshikawa, Hiromu Takemura, Yuka Yamagishi, Hiroshige Mikamo, Hiroyuki Kunishima
Summary: The concentrations of lactobacillus metabolites have varied effects on the suppression of Candida albicans biofilms. Lactate and hydrogen peroxide produced by lactobacillus are both required to inhibit C. albicans growth. Lactobacillus strains that significantly inhibit biofilm formation also inhibit C. albicans adhesion to epithelial cells.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Chemistry, Multidisciplinary
G. Crivello, L. Fracchia, G. Ciardelli, M. Boffito, C. Mattu
Summary: Bacterial infections present a growing threat to healthcare systems. Bacteria often form biofilms, which are dense 3D structures that make eradication difficult and promote antibiotic resistance. Understanding the composition and mechanical properties of biofilms is crucial for developing effective in vitro models. This review provides an overview of biofilm features, explores factors influencing biofilm composition, and presents various in vitro biofilm models, comparing their advantages and disadvantages.
Article
Cell Biology
Vanessa Mancini, Alexandra C. Schrimpe-Rutledge, Simona G. Codreanu, Stacy D. Sherrod, John A. McLean, Helen M. Picton, Virginia Pensabene
Summary: This study utilized a microfluidic system to evaluate the metabolomics differences in murine embryos cultured with endometrial cells-conditioned media (CM). The results showed that CM significantly increased blastocyst rates and enhanced the abundance of metabolites involved in key metabolic pathways, suggesting a beneficial effect on embryo development. Further investigations are needed to identify specific pathways involved in embryo development and implantation.
Article
Chemistry, Analytical
Robert Knitsch, Mohammad AlWahsh, Hannes Raschke, Joerg Lambert, Roland Hergenroeder
Summary: Three-dimensional cell cultures are becoming increasingly important in biochemical research, requiring adaptations of established analytical techniques for more accurate representation of tissue features. This study presents an NMR-based approach to obtain dynamically radial metabolite profiles for cell spheroids, one of the most frequently used 3D models, using spatially selective NMR pulse sequences and a commercially available cryogenic NMR probe.
ANALYTICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Jessica C. Orr, Robert E. Hynds
Summary: Recent advances in stem cell biology and understanding of factors determining lung stem cell self-renewal have facilitated long-term in vitro culture of human lung cells. Improved capability to expand and study primary cells, including in clonal cultures, opens up possibilities for addressing fundamental questions about lung homeostasis and repair, as well as translational questions in various lung diseases.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Biochemical Research Methods
Bisan Samara, Muhammedin Deliorman, Pavithra Sukumar, Mohammad A. Qasaimeh
Summary: A method of creating cryopreservable high throughput arrays of 3D tumor models using a paper platform is presented, utilizing virtual microwells to facilitate cell aggregation and prevent mixing, allowing for extended storage and drug treatment studies.
Review
Chemistry, Analytical
Xiaoting Guo, Qiuxia Shen, Ziyu Chen, Ziyi He, Xianghua Yan
Summary: This review provides an overview of microfluidic technology for bacterial single-cell analysis in mammals. It discusses single-bacterial compartmentalization strategies and detection techniques in microfluidic systems, and highlights recent advances in single-bacterial microfluidics from nine aspects. It also discusses current challenges and future perspectives for improving microfluidic technology for bacterial single-cell analysis. This review will contribute to a better understanding of the evolutionary and functional diversity of mammalian microbiomes.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Shamapto Guha Anthon, Karolina Papera Valente
Summary: Traditional 2D cell culture models fail to accurately replicate the cellular environment in vivo, leading to discrepancies between preclinical and in vivo testing. Recent advancements in tissue engineering have enabled the development of 3D cell culture systems that can accurately mimic the cellular and tissue microenvironment. However, the lack of perfusion systems limits their application as drug screening platforms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Pharmacology & Pharmacy
Patricia Zoio, Abel Oliva
Summary: The increased demand for physiologically relevant in vitro human skin models for testing pharmaceutical drugs has led to significant advancements in skin engineering. One promising approach is the use of in vitro microfluidic systems, known as skin-on-a-chip devices, which allow the simulation of key features of human skin. These devices can perfuse the skin tissue and integrate sensors for real-time monitoring of skin function and the effects of drugs.
Article
Obstetrics & Gynecology
Mary E. Salliss, Leslie Farland, Nichole D. Mahnert, Melissa M. Herbst-Kralovetz
Summary: The relationship between the microbiome and endometriosis, infertility, and chronic pelvic pain (CPP) is complex and requires further research for a better understanding. Studies have shown a potential link between dysbiotic gut or genital microbiota and gynecologic conditions, highlighting the need for more rigorous study designs and consistent methodology across the field.
HUMAN REPRODUCTION UPDATE
(2022)
Article
Immunology
Allison N. Dammann, Anna B. Chamby, Francisco J. Gonzalez, Molly E. Sharp, Karina Flores, Ifrah Shahi, Sophia Dongas, Thomas A. Hooven, Adam J. Ratner
Summary: This study found that specific capsule types may provide an advantage in GBS vaginal colonization, but the success of certain GBS lineages may involve both capsule and non-capsule genetic elements.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Biochemical Research Methods
Nicholas A. Bokulich, Pawel Laniewski, Anja Adamov, Dana M. Chase, J. Gregory Caporaso, Melissa M. Herbst-Kralovetz
Summary: This study provides insights into the interactions between vaginal microbiome, metabolome, and genital inflammation in cervical neoplasms. The integration of multi-omics data reveals the characteristic features and predictive models of the cervicovaginal microenvironment. These findings highlight the importance of multi-omics approaches in microbiome research and the potential contribution of host-microbe interactions to cervical carcinogenesis.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Karen Lithgow, Vienna C. H. Buchholz, Emily Ku, Shaelen Konschuh, Ana D'Aubeterre, Laura K. Sycuro
Summary: Porphyromonas asaccharolytica and Porphyromonas uenonis are common inhabitants of the vaginal microbiome, linked to adverse health outcomes for women. These species secrete broad-acting proteases that degrade collagens in cervix and chorioamniotic membranes, as well as fibrinogen, potentially harming human pregnancy through clotting disruption, fetal membrane weakening, and premature cervical remodeling.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Editorial Material
Medicine, General & Internal
Perri Klass, Adam J. Ratner
Summary: The article discusses Peter Ludvig Panum's investigation of a measles epidemic in the Faroe Islands in 1846, highlighting the unique geography and isolated population that facilitated the analysis of disease spread. It also mentions the potential lessons for studying Covid-19 in the Faroes today.
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Editorial Material
Microbiology
Pawel Laniewski, Melissa M. Herbst-Kralovetz
NATURE MICROBIOLOGY
(2022)
Article
Microbiology
Emily A. Kaelin, Peter T. Skidmore, Pawel Laniewski, LaRinda A. Holland, Dana M. Chase, Melissa M. Herbst-Kralovetz, Efrem S. Lim
Summary: The link between the cervicovaginal bacterial microbiome, HPV infection, and cervical cancer provides insight into the development of cervical cancer. However, the role of the cervicovaginal virome remains poorly understood.
Article
Biology
Jason D. Maarsingh, Pawel Laniewski, Melissa M. Herbst-Kralovetz
Summary: Specific bacteria of the human microbiome can influence carcinogenesis at different anatomical sites. Bacterial vaginosis (BV)-associated bacteria may facilitate a tumor-promoting microenvironment in gynecologic cancers.
COMMUNICATIONS BIOLOGY
(2022)
Article
Medicine, General & Internal
Marie E. Wang, Adam J. Ratner
JOURNAL OF HOSPITAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Felicia Kuperwaser, Gal Avital, Michelle J. Vaz, Kristen N. Noble, Allison N. Dammann, Tara M. Randis, David M. Aronoff, Adam J. Ratner, Itai Yanai
Summary: Group B Streptococcus (GBS) is a pathobiont that ascends to the placenta and causes adverse pregnancy outcomes by producing the toxin beta-hemolysin/cytolysin (beta-h/c). This study shows that GBS modulates the state of innate immune cells by subverting host inflammation through beta-h/c, leading to worse outcomes.
MOLECULAR SYSTEMS BIOLOGY
(2023)
Article
Microbiology
Nicole R. R. Jimenez, Jason D. D. Maarsingh, Pawel Laniewski, Melissa M. M. Herbst-Kralovetz
Summary: The vaginal microbiome in reproductive-age women is mainly composed of certain Lactobacillus species, which are associated with optimal cervicovaginal health. However, the metabolic contributions of other lactobacilli to cervicovaginal health are still not well understood.
Article
Multidisciplinary Sciences
Georgia M. Lorentzen, Pawel Laniewski, Haiyan Cui, Denise J. Rose, Jamal Mouard, Nichole D. Marnert, Leslie Farland, Melissa M. Herbst-Kralovetz
Summary: Adenomyosis is a burdensome gynecologic condition that often goes undiagnosed despite its negative impact on quality of life. In this study, we used non-invasive cervicovaginal lavage samples to analyze the levels of immune proteins and metabolites in women with adenomyosis. We found significant alterations in immune proteins and a large quantity of metabolites related to cytokines and amino acids. Through enrichment analysis, we identified upregulated pathways in adenomyosis related to pyrimidine metabolism, carnitine synthesis, and histidine/histamine metabolism. This study demonstrates the usefulness of non-invasive sampling combined with immunometabolic profiling for adenomyosis detection and a better understanding of its pathophysiology.
Article
Immunology
Sarah Geoghegan, Francia Acosta, Laura C. Stephens, Hanah Gillan, Sandra Valera, Richard J. Drew, Maeve Eogan, Adam J. Ratner, Andrew P. Steenhoff, Karina M. Butler, Kristen A. Feemster
Summary: This study investigated the attitudes of maternity care providers towards the introduction of a GBS vaccine in three countries, showing variability in provider attitudes influenced by geographic and provider type. There is an opportunity to improve awareness and recommendation for GBS vaccine through targeted educational efforts with antenatal providers.
Article
Infectious Diseases
Michal Paret, Rebecca Trillo, Jennifer Lighter, Ilan Youngster, Adam J. Ratner, Rebecca Pellett Madan
Summary: Large outbreaks of measles occurred in Israel and New York City during 2018-2019, mainly due to travel of unimmunized children between the two communities. The majority of measles cases occurred in eligible but unvaccinated children. Moreover, over 25% of children in both measles-positive and -negative groups remained unimmunized for measles, mumps, and rubella (MMR) vaccine one year after the outbreak.
JOURNAL OF THE PEDIATRIC INFECTIOUS DISEASES SOCIETY
(2022)
Article
Microbiology
Ifrah Shahi, Cristina N. Llaneras, Sofya S. Perelman, Victor J. Torres, Adam J. Ratner
Summary: This study conducted a CRISPR-Cas9 forward genetic screen to identify host genes involved in beta hc pore formation and cell death, but no clear candidate genes were found. The results suggest that beta hc may not require a single nonessential host factor for cell death.
MICROBIOLOGY SPECTRUM
(2022)
