Article
Biochemistry & Molecular Biology
Lisa G. M. Huis In 't Veld, Nataschja Ho, Melisssa Wassink, Martijn H. den Brok, Gosse J. Adema
Summary: Saponin-based adjuvants (SBAs) are a promising new type of adjuvant that can enhance dendritic cell-mediated antigen cross-presentation and activate CD8 + T cells by inducing lipid bodies. The study reveals that SBAs specifically induce the PERK pathway and depend on its activation for dendritic cell cross-presentation. Additionally, the MHCII(lo)CD11b(hi) subset of dendritic cells shows the highest responsiveness to SBAs.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Immunology
Yuchao Jing, Min Cao, Bei Zhang, Xuehui Long, Xiaoming Wang
Summary: The testis is an immune privilege site containing various types of immune cells, with T cells with memory phenotypes being the most abundant. Testicular T cells play important roles in immune regulation under different physiological states.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
America Chandia-Cristi, Nicolas Stuardo, Cristian Trejos, Nancy Leal, Daniela Urrutia, Francisca C. Bronfman, Alejandra Alvarez Rojas
Summary: Brain-derived neurotrophic factor (BDNF) activates TrkB receptor and downstream pathways, promoting neuronal survival, growth, and plasticity. The role of c-Abl, a non-receptor tyrosine kinase, in BDNF-induced dendritic arborization was examined using various strategies. Surprisingly, BDNF promoted c-Abl activation and interaction with TrkB receptors. Inhibition or genetic ablation of c-Abl abolished BDNF-induced dendritic arborization without affecting classic TrkB downstream pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Tim B. Fessenden, Lauren E. Stopfer, Fiona Chatterjee, Julian Zulueta, Josh Mesfin, Therese Cordero Dumit, Irene Reijers, Esmee P. Hoefsmit, Christian Blank, Forest White, Stefani Spranger
Summary: Cytotoxic CD8(+) T cells must recognize tumor-derived antigens to achieve effective tumor elimination. Our study shows that dendritic cells induce cytotoxic CD8(+) T-cell responses by cross-presenting tumor-derived peptides, and the proportion of membrane-derived neoantigens is associated with reduced survival and treatment response.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Editorial Material
Immunology
Remi Hatinguais, Gordon D. Brown
Summary: After internalizing an actin-bearing ligand, the C-type lectin receptor DNGR-1 promotes phagosome rupture, allowing antigens to enter the cytosol for processing through the MHC-I pathway.
Article
Multidisciplinary Sciences
Jingyi Li, Tao Zhang, Tao Ren, Xiaoyu Liao, Yilong Hao, Je Sun Lim, Jong-Ho Lee, Mi Li, Jichun Shao, Rui Liu
Summary: This study identifies an oxygen-sensitive methylation of ULK1 with an important role in hypoxic stress adaptation by promoting autophagy induction.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Xin Wu, Yong Tang, Silin Zhang, Xueqiang Zhao, Xin Lin
Summary: This study reveals the essential role of HOIP in maintaining dendritic cell homeostasis. HOIP deficiency leads to spontaneous inflammation, and MyD88-dependent proinflammatory signal significantly contributes to the pathogenesis of autoinflammation associated with HOIP mutations.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Cell Biology
Junji Xing, Xiaojing Zhou, Mingli Fang, Evan Zhang, Laurie J. Minze, Zhiqiang Zhang
Summary: The study reveals the critical role of RNA helicase DHX15 in sensing enteric RNA viruses and controlling intestinal inflammation in intestinal epithelial cells, through triggering NLRP6 inflammasome assembly and IL-18 secretion.
Review
Medicine, Research & Experimental
Tingting Zhang, Adila Aipire, Yijie Li, Changying Guo, Jinyao Li
Summary: This review summarizes the mechanisms of cross-presentation (XPT) and its application in tumor immunotherapy. Dendritic cells enhance their antigen presentation capacity through receptor-mediated internalization of exogenous antigens, endosome escape, engagement of other XPT-related proteins, and the use of adjuvants, thereby improving the outcomes of DC-based therapy.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Immunology
Xinxin Li, Siya Liu, Kul Raj Rai, Wenzhuo Zhou, Song Wang, Xiaojuan Chi, Guijie Guo, Ji-Long Chen, Shasha Liu
Summary: STAT2, an important transcription factor, is phosphorylated upon viral infection by multiple viruses, including influenza A virus, in an early infection stage. This initial activation of STAT2 is regulated by the RIG-I/MAVS-dependent pathway and is independent of type I IFNs and JAK kinases. Inhibition of STAT2 phosphorylation at Tyr690 compromises antiviral response and facilitates viral replication. MAPK12 and Syk kinases are involved in the early phosphorylation of STAT2 triggered by influenza A virus infection. Inhibition of MAPK12 accelerates influenza virus replication in host.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Louis Lo, Oro Uchenunu, Roberto J. Botelho, Costin N. Antonescu, Raffi Karshafian
Summary: Ultrasound-stimulated microbubble (USMB) treatment is a promising strategy for cancer therapy, promoting drug delivery and impairing cell viability. However, it has heterogeneous effects on cell viability, allowing some cells to survive and/or proliferate through adaptive mechanisms.
Article
Immunology
Gaetan Barbet, Priyanka Nair-Gupta, Michael Schotsaert, Stephen T. Yeung, Julien Moretti, Fabian Seyffer, Giorgi Metreveli, Thomas Gardner, Angela Choi, Domenico Tortorella, Robert Tampe, Kamal M. Khanna, Adolfo Garcia-Sastre, J. Magarian Blander
Summary: The study explains how viruses target host cell antigen-processing pathways, focusing on MHC-I molecules and the re-routing of ERGIC-resident MHC molecules to phagosomal vesicles for cross-presentation. It suggests that even without TAP, protective CD8(+) T cells can still be mobilized during viral infection, highlighting a noncanonical cross-presentation pathway.
Review
Immunology
Ray A. Ohara, Kenneth M. Murphy
Summary: Cross-priming, initially recognized in cytotoxic T lymphocyte (CTL) responses, involves the presentation of minor histocompatibility antigens by antigen presenting cells (APCs) derived from immunizing cells. As understanding of T cell receptor antigen recognition progressed, cross-priming was redefined as cross-presentation and expanded to include different forms of antigens and APCs not involved in in vivo CTL priming. In vitro cell models have been utilized for studying cross-presentation, but recent studies have shown differences between these models and in vivo APCs. Current research focuses on validating in vivo pathways and gene candidates for cross-presentation, and evaluating their contributions to CTL responses across different antigens and immunologic settings.
SEMINARS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Liu Yang, Haiyan Yang, Yunxiang Chu, Yunhao Song, Lidan Ding, Bingtao Zhu, Wanli Zhai, Xuning Wang, Yanshen Kuang, Fangli Ren, Baoqing Jia, Wei Wu, Xiongjun Ye, Yinyin Wang, Zhijie Chang
Summary: This study reveals the essential role of CREPT in maintaining murine intestinal stem cells, with its deficiency impairing cell proliferation and regeneration. The downregulation of Wnt signaling due to CREPT deletion is identified as a key mechanism affecting ISC function.
NATURE COMMUNICATIONS
(2021)
Article
Cell & Tissue Engineering
Jean-Marie Jacob, Selene E. Di Carlo, Igor Stzepourginski, Anthony Lepelletier, Papa Diogop Ndiaye, Hugo Varet, Rachel Legendre, Etienne Kornobis, Adam Benabid, Giulia Nigro, Lucie Peduto
Summary: The transcriptomic switch induced by PDGFR alpha in intestinal stromal cells is crucial for coordinating postnatal intestinal maturation and function.
Article
Oncology
Benjamin L. Green, Michael G. House
SURGICAL ONCOLOGY CLINICS OF NORTH AMERICA
(2019)
Article
Rheumatology
Ryan J. Pachucki, Chelsea Corradetti, Lynne Kohler, Jay Ghadiali, Paul M. Gallo, Lauren Nicastro, Sarah A. Tursi, Stefania Gallucci, Cagla Tukel, Roberto Caricchio
ARTHRITIS & RHEUMATOLOGY
(2020)
Article
Rheumatology
Roberto Caricchio, Marcello Gallucci, Chandra Dass, Xinyan Zhang, Stefania Gallucci, David Fleece, Michael Bromberg, Gerard J. Criner
Summary: Researchers developed predictive criteria for COVID-19-associated cytokine storm (CS) and proposed new early predictive criteria to identify CS occurring in patients with COVID-19, which can determine the need for early therapeutic regimen and potentially reduce mortality.
ANNALS OF THE RHEUMATIC DISEASES
(2021)
Review
Biochemistry & Molecular Biology
Stefania Gallucci, Sowmya Meka, Ana M. Gamero
Summary: Type I interferons (IFNs) play a crucial role in the pathogenesis of Systemic Lupus Erythematosus (SLE), and aberrant IFN signaling can exacerbate SLE. Genetic and epigenetic abnormalities are present in SLE patients. Neutralizing anti-type I IFN receptor antibodies may be a therapeutic strategy.
Article
Medicine, Research & Experimental
Tam D. Quach, Weiqing Huang, Ranjit Sahu, Catherine M. M. Diadhiou, Chirag Raparia, Roshawn Johnson, Tung Ming Leung, Susan Malkiel, Peta Gay Ricketts, Stefania Gallucci, Cagla Tukel, Chaim O. Jacob, Martin L. Lesser, Yong-Rui Zou, Anne Davidson
Summary: TNF inhibitors used for treating inflammatory diseases may cause the development of new autoantibodies and secondary autoimmune diseases. TNF deficiency can induce the production of TH17 T cells and enhance the production of germline encoded, T-dependent IgG anti-cardiolipin antibodies. However, it does not induce germinal center formation or precipitate clinical disease. Additional immune stimuli can restore germinal center formation and induce pathogenic autoimmunity in TNF-deficient mice via extrafollicular pathways or atypical germinal center-like pathways.
Article
Multidisciplinary Sciences
John C. McVey, Benjamin L. Green, Benjamin Ruf, Justin D. McCallen, Simon Wabitsch, Varun Subramanyam, Laurence P. Diggs, Bernd Heinrich, Tim F. Greten, Chi Ma
Summary: Non-alcoholic fatty liver disease (NAFLD) has a significant impact on liver cancer development and may impair antigen-specific CD8 (+) T cell immunity due to accumulated macrophages in the liver environment.
Article
Gastroenterology & Hepatology
Simon Wabitsch, Justin D. McCallen, Olena Kamenyeva, Benjamin Ruf, John C. McVey, Juraj Kabat, Juliane S. Walz, Yaron Rotman, Kylynda C. Bauer, Amanda J. Craig, Marie Pouzolles, Ira Phadke, Vanessa Catania, Benjamin L. Green, Claude Fu, Laurence P. Diggs, Bernd Heinrich, Xin Wei Wang, Chi Ma, Tim F. Greten
Summary: Non-alcoholic steatohepatitis (NASH) negatively affects the efficacy of immune checkpoint inhibitor (ICI) therapy in hepatocellular carcinoma (HCC). This study reveals that NASH alters the metabolism, function, and motility of hepatic CD8+ T cells, leading to reduced responsiveness to anti-PD-1 treatment. The efficacy can be rescued by metformin treatment.
JOURNAL OF HEPATOLOGY
(2022)
Letter
Oncology
Martha E. Teke, Lauren A. Gamble, Sarah G. Samaranayake, Benjamin L. Green, Areeba Saif, Jonathan M. Hernandez, Jeremy L. Davis
ANNALS OF SURGICAL ONCOLOGY
(2022)
Article
Endocrinology & Metabolism
Benjamin L. Green, Robert R. C. Grant, Christopher T. Richie, Bishwanath Chatterjee, Michelly Sampaio De Melo, Frederic G. Barr, Karel Pacak, Sunita K. Agarwal, Naris Nilubol
Summary: This study reports the discovery and characterization of a novel BRAF-containing fusion transcript in a patient with recurrent PCC, leading to activation of the MAPK signaling pathway and upregulation of EMT markers. The findings suggest potential benefits of clinically available MEK and/or BRAF inhibitors for this patient.
EUROPEAN JOURNAL OF ENDOCRINOLOGY
(2022)
Article
Genetics & Heredity
Benjamin L. Green, Grace-Ann Fasaye, Sarah G. Samaranayake, Anna Duemler, Lauren A. Gamble, Jeremy L. Davis
Summary: Pathogenic and likely pathogenic variants in the CDH1 gene are associated with increased risk of gastric and breast cancers, as well as hereditary cleft lip and palate (CLP). This study aimed to determine the prevalence of CLP in families with these variants. The results showed a high prevalence of CLP in families carrying CDH1 variants, with no clear genotype-phenotype pattern. Genetic testing for CDH1 should be considered in families with CLP and a history of gastric or lobular breast cancer.
FRONTIERS IN GENETICS
(2022)
Article
Oncology
Benjamin L. Green, Alisa N. Blumenthaler, Lauren A. Gamble, James D. McDonald, Kristen Robinson, Maureen Connolly, Monica Epstein, Jonathan M. Hernandez, Andrew M. Blakely, Brian D. Badgwell, Jeremy L. Davis
Summary: CRS-HIPEC treatment for gastric adenocarcinoma with peritoneal metastasis showed a 3-year survival rate of 22% and common complications. The number of lymph node metastases was inversely correlated with overall survival.
ANNALS OF SURGICAL ONCOLOGY
(2023)
Article
Gastroenterology & Hepatology
Benjamin L. Green, Yuta Myojin, Chi Ma, Benjamin Ruf, Lichun Ma, Qianfei Zhang, Umberto Rosato, Jonathan Qi, Mahler Revsine, Simon Wabitsch, Kylynda Bauer, Mohamed-Reda Benmebarek, Justin Mccallen, Amran Nur, Xin Wang, Vivek Sehra, Revant Gupta, Manfred Claassen, Xin Wei Wang, Firouzeh Korangy, Tim F. Greten
Summary: CD29(+) Tregs constitute a unique subpopulation of hepatic Tregs that are highly proliferative and play a critical role in mediating resistance to immune checkpoint inhibitor therapy. They respond to alpha PD-1 and double in size, regardless of the presence of an intrahepatic tumor. IL2 is identified as a critical modulator of both CD29(+) and CD29(-) hepatic Tregs, but IL2-independent expansion of the liver Treg population is driven by CD29(+) Tregs.
Article
Medicine, Research & Experimental
Xinjun Lu, Shanshan Deng, Jiejie Xu, Benjamin L. Green, Honghua Zhang, Guofei Cui, Yi Zhou, Yi Zhang, Hongwei Xu, Fapeng Zhang, Rui Mao, Sheng Zhong, Thorsten Cramer, Matthias Evert, Diego F. Calvisi, Yukai He, Chao Liu, Xin Chen
Summary: Combining AFP vaccine with immune checkpoint inhibitors may be effective for treating AFP (+) HCC patients who do not respond to first-line treatment.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Oncology
Benjamin L. Green, Alisa N. Blumenthaler, Lauren A. Gamble, James D. McDonald, Kristen Robinson, Maureen Connolly, Monica Epstein, Jonathan M. Hernandez, Andrew M. Blakely, Brian D. Badgwell, Jeremy L. Davis
Summary: In patients with gastric adenocarcinoma and isolated peritoneal metastasis treated with CRS-HIPEC, the 3-year overall survival rate from CRS-HIPEC was 22%, and complications were common. The number of pathologic lymph node metastases was inversely correlated with overall survival.
ANNALS OF SURGICAL ONCOLOGY
(2023)
Article
Gastroenterology & Hepatology
Simon Wabitsch, Mayank Tandon, Benjamin Ruf, Qianfei Zhang, Justin D. McCallen, John C. McVey, Chi Ma, Benjamin L. Green, Laurence P. Diggs, Bernd Heinrich, Tim F. Greten
Summary: The study demonstrated that trametinib enhances the immunogenicity of tumor cells by up-regulating MHC-I surface expression and when combined with anti-PD-1, resulted in optimal treatment efficacy for iCCA, particularly in SB1 tumor models. Whole-exome sequencing of SB1 cells suggests that even KRAS wild-type iCCAs respond well to this combination therapy.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)