Article
Microbiology
Tong Wu, Xiaozhen Wang, Yue Dong, Chen Xing, Xueying Chen, Lu Li, Chunliu Dong, Yanhua Li
Summary: In this study, an untargeted metabolomics analysis revealed that the serine catabolic pathway was inhibited in drug-resistant S. suis. The addition of L-serine restored the fungicidal effect of macrolides on S. suis by enhancing the serine metabolic pathway.
MICROBIOLOGY SPECTRUM
(2022)
Review
Microbiology
Muriel Dresen, Peter Valentin-Weigand, Yenehiwot Berhanu Weldearegay
Summary: This review focuses on the role of Streptococcus suis metabolism in adapting to different in vivo host niches, as well as its link to virulence. The potential of targeting metabolic pathways for new therapeutic strategies is also discussed.
Article
Microbiology
Haotian Li, Tingting Li, Qiao Hu, Zhiming Yao, Lu Li, Qi Huang, Rui Zhou
Summary: This study identifies the essential roles of serine/threonine kinases (STKs) in the physiology and pathogenicity of Streptococcus suis, an important bacterial pathogen, and identifies a series of inhibitors against the STKs as potential antimicrobial lead compounds.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Lude Wang, Xiaoya Zhao, Jianfei Fu, Wenxia Xu, Jianlie Yuan
Summary: Cisplatin is a commonly used chemotherapy drug in cancer treatment. However, the drug resistance of tumor cells often leads to treatment failure. Metabolic reprogramming of tumor cells plays a crucial role in tumorigenesis and development.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Gabriele Stankeviciute, Peijun Tang, Ben Ashley, Joshua D. Chamberlain, Matthew E. B. Hansen, Aimiyah Coleman, Rachel D'Emilia, Larina Fu, Eric C. Mohan, Hung Nguyen, Ziqiang Guan, Dominic J. Campopiano, Eric A. Klein
Summary: The bacterial domain produces a variety of sphingolipids with different functions, which play important roles in modulating the host inflammatory system in the human microbiome. Through genomic and biochemical approaches, a complete pathway for bacterial ceramide synthesis was identified, with further discovery of a Gram-positive species capable of producing ceramides. Biochemical evidence suggests that the bacterial ceramide synthesis pathway operates differently from that in eukaryotes, and phylogenetic analyses support the independent evolution of bacterial and eukaryotic ceramide pathways.
NATURE CHEMICAL BIOLOGY
(2022)
Article
Immunology
Dominic Dolbec, Melanie Lehoux, Masatoshi Okura, Daisuke Takamatsu, Marcelo Gottschalk, Mariela Segura
Summary: In this study, we compared the protective properties of different serotypes of Streptococcus suis using serotype-switched mutants in a mouse infection model. The structure of the capsular polysaccharide (CPS) influenced bacterial survival, antibody binding, and antibody-mediated bacterial killing. CPS of serotypes 3, 4, and 14 allowed more antibody binding and bacterial elimination compared to serotypes 2, 7, and 9. These findings suggest that the varying CPS structures of S. suis provide different levels of protection by affecting antigen availability and elimination by the host immune system.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Mei-Fang Tan, Jia Tan, Fan-Fan Zhang, Hai-Qin Li, Hua-Yuan Ji, Shao-Pei Fang, Cheng-Cheng Wu, Yu-Ling Rao, Yan-Bin Zeng, Qun Yang
Summary: This study investigated the pleiotropic effects of exogenous glycogen on Streptococcus suis serotype 2 (SS2) through transcriptome sequencing. The results showed that glycogen induction significantly influenced the metabolic pathways and virulence-associated factors of SS2. Extracellular glycogen utilization enhanced the hemolytic activity, adhesion and invasion ability, and pathogenicity of SS2. These findings are important for understanding the adaptation and pathogenicity mechanisms of SS2.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Yongxia Zhao, Xiaochun Ye, Zhifeng Xiong, Awais Ihsan, Irma Ares, Marta Martinez, Bernardo Lopez-Torres, Maria-Rosa Martinez-Larranaga, Arturo Anadon, Xu Wang, Maria-Aranzazu Martinez
Summary: This review summarizes the role of increased ROS in DNA damage and the apoptosis of cancer cells caused by changes in cancer cell metabolism, as well as various anticancer therapies targeting ROS generation, in order to provide references for cancer therapies based on ROS generation.
Article
Microbiology
Sophie oehlmann, Ann-Kathrin Krieger, Nicolas Gisch, Marita Meurer, Nicole de Buhr, Maren von Koeckritz-Blickwede, Nicole Schuetze, Christoph Georg Baums
Summary: This study demonstrates that Streptococcus suis specifically modulates its interaction with host immune cells by modifying its cell surface.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Qiankun Bai, Ruhui Fan, Ningyuan Zhong, Jianan Liu, Xinming Pan, Huochun Yao, Jiale Ma
Summary: This study explores the role of Pentraxin 3 (PTX3) during Streptococcus suis serotype 2 (SS2) infection and verifies its potential as a new and effective biological agent. The results showed that PTX3 facilitates phagocytosis, inflammatory cell recruitment, and cytokine IL-6 release, contributing to bacterial clearance during SS2 infection. The presence of capsular polysaccharide of SS2 (CPS2) is required for PTX3 to trigger the inflammatory response, suggesting PTX3 as a potential biological agent for prevention and treatment of SS2-related streptococcosis.
VETERINARY SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Soe Maung Maung Phone Myint, Liou Y. Sun
Summary: L-serine is a crucial amino acid involved in protein synthesis, cell proliferation, development, and sphingolipid formation in the central nervous system. It has neuroprotective and anti-inflammatory effects by activating glycine receptors and upregulating PPAR-γ. L-serine has potential as a protective agent in various neurological diseases and neurodegenerative disorders.
Article
Microbiology
Xianfeng Hui, Zhongmin Xu, Lei Cao, Liang Liu, Xian Lin, Yong Yang, Xiaomei Sun, Qiang Zhang, Meilin Jin
Summary: The protein HP0487 of Streptococcus suis serotype 2 was found to play a significant role in adhesion and anti-phagocytosis to neutrophils, influencing the pathogenicity and virulence of SS2.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Haodan Zhu, Junming Zhou, Dandan Wang, Zhengyu Yu, Bin Li, Yanxiu Ni, Kongwang He
Summary: The eukaryotic-type serine/threonine kinase of Streptococcus suis serotype 2 (SS2) plays critical roles in bacterial pathogenesis. Protein profile analysis revealed that STK regulates the expression of proteins involved in virulence of SS2 and its adaption to stress environments.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Microbiology
Weiyi Li, Yifan Yin, Yu Meng, Zhe Ma, Huixing Lin, Hongjie Fan
Summary: In this study, phosphoproteomic analysis identified GlmM as a substrate of STK, with phosphorylation at the Ser-101 site impacting its enzyme activity and cell wall peptidoglycan synthesis. Mutants lacking glmM or with a S101A point mutation showed aberrant cell morphology, attenuated virulence, and reduced enzyme activities and peptidoglycan concentrations. These findings demonstrated that STK phosphorylates GlmM to regulate SS2 pathogenicity.
VETERINARY MICROBIOLOGY
(2021)
Article
Genetics & Heredity
Suhao Han, Austin Sims, Anthony Aceto, Brigitte E. F. Schmidt, Marcel P. P. Bruchez, Aditi U. U. Gurkar
Summary: Oxidative DNA damage increases with age in all tissues, but to varying degrees. Researchers have developed a chemoptogenetic tool in Caenorhabditis elegans that can induce oxidative DNA damage and assess its effects on aging. By targeting a specific histone, they were able to induce DNA damage, leading to embryonic lethality, developmental delay, and reduced lifespan. This tool will allow researchers to study the role of DNA damage in aging at an organismal level.