Article
Biochemistry & Molecular Biology
Chiung-Chyi Shen, Shan-hui Hsu, Kai-Bo Chang, Chun-An Yeh, Hsiang-Chun Chang, Cheng-Ming Tang, Yi-Chin Yang, Hsien-Hsu Hsieh, Huey-Shan Hung
Summary: This study demonstrates the superior biological properties and biocompatibility of PEG-HA-AuNP nanocomposites for cell behavior in both MC3T3-E1 cells and MSCs, as well as their potential in promoting anti-inflammatory response and endothelialization when implanted into rats subcutaneously for a period of 1 month.
Article
Chemistry, Analytical
Jose Luis Carreon Gonzalez, Perla Elvia Garcia Casillas, Christian Chapa Gonzalez
Summary: The use of gold nanoparticles coated with silica and PEG has shown potential as drug delivery systems due to their improved stability and drug loading capacity. The coatings significantly enhanced the nanoparticles' ability to deliver drugs and extended their circulation time in the body. These findings suggest that gold nanoparticles coated with silica and PEG could be further developed as targeted and controlled release drug delivery systems.
Article
Medicine, General & Internal
Xiao-Fan Huang, Kai-Fu Chang, Yu-Ling Lin, Kuang-Wen Liao, Chih-Yen Hsiao, Gwo-Tarng Sheu, Nu-Man Tsai
Summary: Breast cancer is the second most common malignancy in women, and finding new therapeutic drugs to meet clinical demand is essential. Research shows that BP and BP/LPPC can inhibit breast cancer cell growth, with the latter enhancing cytotoxicity by stabilizing BP activity and providing endocytic pathways. Additionally, BP/LPPC exhibits a synergistic effect in combination with doxorubicin.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Multidisciplinary Sciences
Barbora Vitovcova, Veronika Skarkova, Radim Havelek, Jiri Soukup, Ananya Pande, Katerina Caltova, Emil Rudolf
Summary: In our study, we found that the repurposed drug flubendazole (FLU) significantly inhibits proliferation and survival of glioblastoma multiforme (GBM) cells. FLU affects microtubule structure and influences tubulins expression. Additionally, FLU decreases activation of STAT3 and leads to upregulation of p53 signaling pathway, resulting in cell cycle arrest and caspase-dependent cell death. These findings suggest FLU as a promising agent for GBM treatment.
SCIENTIFIC REPORTS
(2023)
Article
Biophysics
Guliz Ak, Ayse Unal, Tugba Karakayali, Buket Ozel, Nur Selvi Gunel, Senay Hamarat Sanlier
Summary: The study demonstrates that BCNU and TMZ loaded SLNs can serve as an effective anticancer system for targeted GBM therapy, showing significant antitumor activity and inducing apoptosis on cancer cells, as well as being more readily taken up by MCT-1 expressing brain cells.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Jacqueline L. Pasek-Allen, Randall K. Wilharm, John C. Bischof, Valerie C. Pierre
Summary: The molecular weight, purity, and functionalization of polyethylene glycols are often determined using 1H NMR spectroscopy. However, the commonly used H-1 NMR pulse sequence does not decouple the C-13 nuclei. This leads to erroneous assignments as the C-13 coupled H-1 peaks from the repeating units have similar integrations to those of the H-1 peaks from the terminal groups. Taking into account this coupling allows for more accurate determination of both the molecular weight of the polymer and the efficacy of conjugation of a terminal moiety compared to using the uncoupled H-1 peaks from the repeating unit.
Article
Chemistry, Multidisciplinary
Jacqueline L. Pasek-Allen, Randall K. Wilharm, John C. Bischof, Valerie C. Pierre
Summary: The molecular weight, purity, and functionalization of polyethylene glycols are commonly determined using H-1 NMR spectroscopy. It is important to consider the C-13 coupling in the H-1 NMR pulse sequence for large polymers, as the integrations of the 13C-coupled H-1 peaks from the repeating units can be similar to those of the H-1 peaks from the terminal groups. Ignoring this coupling can lead to incorrect assignments. Once appropriately assigned, these C-13-coupled H-1 peaks can provide more accurate measurements of the polymer's molecular weight and the efficacy of conjugation of a terminal moiety than the uncoupled H-1 peaks from the repeating units.
Review
Oncology
Jen-Fu Hsu, Shih-Ming Chu, Chen-Chu Liao, Chao-Jan Wang, Yi-Shan Wang, Mei-Yin Lai, Hsiao-Chin Wang, Hsuan-Rong Huang, Ming-Horng Tsai
Summary: Glioblastoma multiforme (GBM) is the most common and malignant brain tumor with poor prognosis. The presence of GBM stem cells and the blood brain barrier (BBB) compromise chemotherapy and radiation therapy. Nanotechnology and nanocarrier-based drug delivery offer new possibilities for overcoming GBM treatment resistance.
Article
Chemistry, Medicinal
Masato Maruyama, Haruka Tojo, Keita Toi, Yusuke Ienaka, Kenji Hyodo, Hiroshi Kikuchi, Ken-ichi Ogawara, Kazutaka Higaki
Summary: The effect of doxorubicin release rates from PEG-liposomes on anti-tumor activity was investigated. The study showed that liposomes with a moderate release rate exhibited the highest anti-tumor activity, which was correlated with the distribution of the drug in cancer cells.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Julia Penas-Martinez, Gines Luengo-Gil, Salvador Espin, Nataliya Bohdan, Carmen Ortega-Sabater, Maria Carmen Rodenas, David Zaragoza-Huesca, Maria Jose Lopez-Andreo, Carme Plasencia, Vicente Vicente, Alberto Carmona-Bayonas, Irene Martinez-Martinez
Summary: Our study reveals the versatile anti-tumor properties of prelatent antithrombin on U-87 MG glioblastoma multiforme cells, inhibiting migration and invasion while having a lesser effect on proliferation. Prelatent antithrombin down-regulated the expression of VEGFA, pSTAT3, and pERK1/2 in U-87 MG cells.
Article
Medicine, Research & Experimental
Tien-Ching Chang, Bing-Mae Chen, Jer-Yuan Wu, Tian-Lu Cheng, Steve Roffler
Summary: Antibodies that bind polyethylene glycol (PEG) can accelerate the clearance of pegylated medicines, with higher affinity antibodies leading to greater clearance and reduced drug efficacy. The molar ratio of high affinity anti-PEG antibodies to PEG-EPO correlates with accelerated blood clearance in mice.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Engineering, Biomedical
Shuhan Liu, Ji Liu, Haisong Li, Kuirong Mao, Haorui Wang, Xiandi Meng, Jialiang Wang, Chenxi Wu, Hongmei Chen, Xin Wang, Xiuxiu Cong, Yue Hou, Ye Wang, Ming Wang, Yong-Guang Yang, Tianmeng Sun
Summary: This study develops a novel cationic lipid nanoparticle (LNP) that efficiently delivers siRNA across the blood-brain barrier (BBB) and targets the tumor microenvironment in the brain of mice for immunotherapy of glioblastoma multiforme (GBM). The LNP is effective in delivering siRNA against CD47 and PD-L1 into the cranial GBM in mice, resulting in downregulation of target gene expression and activation of antitumor immunity.
Article
Biochemistry & Molecular Biology
Kittisak Jantanasakulwong, Sarinthip Thanakkasaranee, Phisit Seesuriyachan, Pisith Singjai, Aphisit Saenjaiban, Siriphan Photphroet, Kanticha Pratinthong, Yuthana Phimolsiripol, Noppol Leksawasdi, Thanongsak Chaiyaso, Sarana Rose Sommano, Pensak Jantrawut, Siriwadee Chomdej, Suwit Chotinan, Francisco J. Barba, Joe M. Regenstein, Alissara Reungsang, Pornchai Rachtanapun
Summary: The nano-metal-treated PET films with anti-virus and anti-fogging ability were developed using sparking nano-metal particles of Ag, Zn, and Ti wires on polyethylene terephthalate (PET) films. A highly transparent, anti-fogging, and anti-virus face shield was prepared using the Zn-PET film. Sparking was an effective technique to prepare the alternative anti-virus and anti-fogging films for medical biomaterial applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Zefeng Chen, Yaoxun Zeng, Niping Chen, Mingxia Zhang, Yakun Wang, Zhenxing Pan, Jiongpeng Yuan, Zhaoyi Ye, Xiaojing Li, Wangqing Bian, Haihong Li, Kun Zhang, Yan He, Xujie Liu
Summary: This study proposes a novel approach for the transport of metal ions using polyethylene glycol. By a simple synthesis method, 31 different metal ion-based nanoparticles were successfully synthesized and demonstrated their potential for cancer theranostics.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Pharmacology & Pharmacy
Ben Newland, Johannes Starke, Chiara Bastiancich, Diana P. N. Goncalves, Laura J. Bray, Wenxin Wang, Carsten Werner
Summary: Local drug delivery system can achieve high concentration of therapeutic agents at tumor site, minimizing systemic toxicity. This study developed a well-defined microscale hydrogel incorporating gold nanorods for photothermal therapy. The hydrogel could be loaded with doxorubicin and released it over one week, exhibiting toxicity against glioma cells. Research showed potential for antitumor activity through combining photothermal therapy and drug release.
Article
Medicine, General & Internal
Lih-Ju Chen, Ping-Ju Chen, Shun-Fa Yang, Jia-Yuh Chen
Summary: This study found that Escherichia coli is the most common causative organism in jaundiced infants with significant bacteriuria. Current recommended antibiotic treatment for neonatal urinary tract infection with E. coli shows low sensitivity to ampicillin but high sensitivity to gentamicin. It is suggested to modify the empiric antibiotic treatment for Gram-negative bacterial infections in neonates by changing gentamicin to amikacin.
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
(2022)
Article
Biochemistry & Molecular Biology
Chen-Yi Liao, Ting-Chien Wu, Shun-Fa Yang, Jinghua Tsai Chang
Summary: N-acetylcysteine (NAC) can act as both an antioxidant and a pro-oxidant. The effect of NAC on cell proliferation varies depending on the antioxidant activity of the cells. The sensitivity of cells to NAC is determined by their intracellular ROS levels.
Article
Ophthalmology
Chia-Chen Lin, Chia-Yi Lee, Jing-Yang Huang, Sheng-Min Hsu, Jia-Horung Hung, Shun-Fa Yang
Summary: This study provides an in-depth analysis of the trends in DR, DME, and related comorbidities in patients with T2DM. It shows that the prevalence and incidence of DR and PDR decreased over time, while the prevalence and incidence of DME increased. Additionally, the percentage of patients with hyperlipidaemia increased in all forms of DR, while the percentage of patients with ESRD increased only in DME.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Genetics & Heredity
Chee-Ming Lee, Yi-Sun Yang, Edy Kornelius, Chien-Ning Huang, Min-Yen Hsu, Chia-Yi Lee, Shu-Yen Peng, Shun-Fa Yang
Summary: The SNP rs145204276 Del/Del variant of the GAS5 gene is associated with an increased susceptibility to diabetic retinopathy (DR) in diabetes mellitus (DM) patients, particularly in those with non-proliferative diabetic retinopathy (NPDR).
Article
Genetics & Heredity
Chih-Chun Chuang, Yi-Sun Yang, Edy Kornelius, Chien-Ning Huang, Min-Yen Hsu, Chia-Yi Lee, Shun-Fa Yang
Summary: The T allele of LINC00673 SNP rs11655237 is associated with a higher risk of developing NPDR. Patients with the LINC00673 SNP rs11655237 CT + TT variant have a shorter duration of diabetes mellitus.
FRONTIERS IN GENETICS
(2022)
Article
Pharmacology & Pharmacy
Peace Wun-Ang Lu, Chia-Hsuan Chou, Jia-Sin Yang, Yi-Hsien Hsieh, Meng-Ying Tsai, Ko-Hsiu Lu, Shun-Fa Yang
Summary: HO-3867 induces apoptosis in human osteosarcoma cells through the JNK signaling pathway.
Article
Medicine, General & Internal
Hsien-Cheng Huang, Bei-Hao Shiu, Shih-Chi Su, Chi-Chou Huang, Wen-Chien Ting, Lun-Ching Chang, Shun-Fa Yang, Ying-Erh Chou
Summary: The study suggests that MMP-11 gene polymorphisms may influence the clinicopathological characteristics of colorectal cancer patients, providing potential biomarkers for disease progression evaluation and shedding light on sex differences in CRC development and prognosis.
Article
Environmental Sciences
Chung-Yuan Lee, Yung-Chuan Ho, Chiao-Wen Lin, Min-Chieh Hsin, Po-Hui Wang, Ya-Cheng Tang, Shun-Fa Yang, Yi-Hsuan Hsiao
Summary: The synthetic curcumin analog, EF-24, has been shown to have enhanced bioavailability and stronger anti-inflammatory and anti-cancer activity compared to curcumin. This study investigated the effects of EF-24 on TPA-induced cellular migration of cervical cancer and found that EF-24 significantly reduced cellular migration and invasion. Further analysis revealed that EF-24 modulated MMP-9 expression by inhibiting the phosphorylation of the p38 signaling pathway. These findings suggest that EF-24 may have potential as a chemopreventive agent for cervical cancer.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Ophthalmology
Hsiang-Wen Chien, Chia-Yi Lee, Jing-Yang Huang, Hung-Chi Chen, Kai Wang, Shun-Fa Yang
Summary: This study, using the National Health Insurance Research Database of Taiwan, found a relationship between congenital cytomegalovirus (CMV) infection and late-onset keratitis. Severe congenital CMV infection was found to be significantly correlated with late-onset keratitis in individuals with congenital CMV infection.
EUROPEAN JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ruei-Hong Lin, Hsu-Tung Lee, Chun-An Yeh, Yi-Chin Yang, Chiung-Chyi Shen, Kai-Bo Chang, Bai-Shuan Liu, Hsien-Hsu Hsieh, Hui-Min David Wang, Huey-Shan Hung
Summary: The research shows that using AuNPs at concentrations of 1.25 and 2.5 ppm can enhance cell proliferation, reduce reactive oxygen species generation, and attenuate platelet/monocyte activation. AuNPs can also promote cell adhesion, increase the expression of matrix metalloproteinase (MMP-2/9), decrease cell apoptosis and the release of inflammatory cytokines. Animal model experiments demonstrate that treating with 1.25 and 2.5 ppm AuNPs exhibits better biocompatibility and endothelialization. This suggests that AuNPs can be used as a biocompatible nanomedicine for regeneration research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Wen-Yu Cheng, Meng-Yin Yang, Chun-An Yeh, Yi-Chin Yang, Kai-Bo Chang, Kai-Yuan Chen, Szu-Yuan Liu, Chien-Lun Tang, Chiung-Chyi Shen, Huey-Shan Hung
Summary: This study investigated the biocompatibility, biological performances, and cell uptake efficiency of AuNP in Wharton's jelly mesenchymal stem cells and a rat model. Various concentrations of AuNP were prepared and characterized. In vitro examinations showed that treatment with 1.25 and 2.5 ppm AuNP improved viability, CXCR4 expression, migration distance, and reduced apoptotic-related protein expression in Wharton's jelly MSCs. AuNP-Col was taken up by cells via clathrin-mediated endocytosis and the vacuolar-type H+-ATPase pathway. In vivo examinations demonstrated that 2.5 ppm AuNP attenuated foreign body responses and had better retention efficacy in tissue integrity. Overall, AuNP shows promise as a biosafe nanodrug delivery system for regenerative medicine with Wharton's jelly MSCs.
Article
Cell Biology
Meng-Yin Yang, Cheng-Di Chiu, Yi-Chun Ke, Yi-Chin Yang, Kai-Bo Chang, Chien-Min Chen, Hsu-Tung Lee, Chien-Lun Tang, Bai-Shuan Liu, Huey-Shan Hung
Summary: Au decorated with type I collagen (Col) was used as a core material to cross-link with stromal cell-derived factor 1 alpha (SDF1 alpha) for investigating its biological performance. The physicochemical characteristics of the Au-based nanoparticles were determined using SEM, DLS, UV-Vis, and FTIR. The biocompatibility of the nanoparticles was evaluated using MSCs, and their potential for inducing cell differentiation and transportation in cells was explored.
Article
Cell Biology
Yi-Fang Ding, Yung-Wei Lin, Wen-Kuan Chiu, Chiao-Wen Lin, Yi-Chieh Yang, Lun-Ching Chang, Jungshan Chang, Shun-Fa Yang, Ming-Hsien Chien
Summary: This study investigated the association between genetic variants of HRH1 and OSCC susceptibility and clinicopathologic variables. The results showed that certain genotypes of HRH1 were associated with a lower risk of developing larger tumor sizes, especially in patients with a betelquid-chewing habit. Furthermore, HRH1 expression levels were upregulated in OSCC tissues and correlated with tumor sizes and prognoses.
Article
Cell Biology
Hsueh-Ju Lu, Chun-Yi Chuang, Mu-Kuan Chen, Chun-Wen Su, Wei-En Yang, Chia-Ming Yeh, Kuan-Ming Lai, Chih-Hsin Tang, Chiao-Wen Lin, Shun-Fa Yang
Summary: ALDH7A1 polymorphisms and expression levels are associated with oral cancer development and prognosis, affecting pathological features, cell migration, and patient outcomes.
Article
Integrative & Complementary Medicine
Shih-Chi Su, Yi-Tzu Chen, Yi-Hsien Hsieh, Wei-En Yang, Chun-Wen Su, Wen-Yu Chiu, Shun-Fa Yang, Chiao-Wen Lin
Summary: Gambogic acid can inhibit cell proliferation and induce apoptosis in oral squamous cell carcinoma by activating the p38-dependent apoptotic pathway. These findings provide potential directions for the development of new treatments for oral cancer.
AMERICAN JOURNAL OF CHINESE MEDICINE
(2022)