Article
Biochemistry & Molecular Biology
Chiung-Chyi Shen, Shan-hui Hsu, Kai-Bo Chang, Chun-An Yeh, Hsiang-Chun Chang, Cheng-Ming Tang, Yi-Chin Yang, Hsien-Hsu Hsieh, Huey-Shan Hung
Summary: This study demonstrates the superior biological properties and biocompatibility of PEG-HA-AuNP nanocomposites for cell behavior in both MC3T3-E1 cells and MSCs, as well as their potential in promoting anti-inflammatory response and endothelialization when implanted into rats subcutaneously for a period of 1 month.
Article
Chemistry, Analytical
Jose Luis Carreon Gonzalez, Perla Elvia Garcia Casillas, Christian Chapa Gonzalez
Summary: The use of gold nanoparticles coated with silica and PEG has shown potential as drug delivery systems due to their improved stability and drug loading capacity. The coatings significantly enhanced the nanoparticles' ability to deliver drugs and extended their circulation time in the body. These findings suggest that gold nanoparticles coated with silica and PEG could be further developed as targeted and controlled release drug delivery systems.
Article
Medicine, General & Internal
Xiao-Fan Huang, Kai-Fu Chang, Yu-Ling Lin, Kuang-Wen Liao, Chih-Yen Hsiao, Gwo-Tarng Sheu, Nu-Man Tsai
Summary: Breast cancer is the second most common malignancy in women, and finding new therapeutic drugs to meet clinical demand is essential. Research shows that BP and BP/LPPC can inhibit breast cancer cell growth, with the latter enhancing cytotoxicity by stabilizing BP activity and providing endocytic pathways. Additionally, BP/LPPC exhibits a synergistic effect in combination with doxorubicin.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Multidisciplinary Sciences
Barbora Vitovcova, Veronika Skarkova, Radim Havelek, Jiri Soukup, Ananya Pande, Katerina Caltova, Emil Rudolf
Summary: In our study, we found that the repurposed drug flubendazole (FLU) significantly inhibits proliferation and survival of glioblastoma multiforme (GBM) cells. FLU affects microtubule structure and influences tubulins expression. Additionally, FLU decreases activation of STAT3 and leads to upregulation of p53 signaling pathway, resulting in cell cycle arrest and caspase-dependent cell death. These findings suggest FLU as a promising agent for GBM treatment.
SCIENTIFIC REPORTS
(2023)
Article
Biophysics
Guliz Ak, Ayse Unal, Tugba Karakayali, Buket Ozel, Nur Selvi Gunel, Senay Hamarat Sanlier
Summary: The study demonstrates that BCNU and TMZ loaded SLNs can serve as an effective anticancer system for targeted GBM therapy, showing significant antitumor activity and inducing apoptosis on cancer cells, as well as being more readily taken up by MCT-1 expressing brain cells.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Jacqueline L. Pasek-Allen, Randall K. Wilharm, John C. Bischof, Valerie C. Pierre
Summary: The molecular weight, purity, and functionalization of polyethylene glycols are often determined using 1H NMR spectroscopy. However, the commonly used H-1 NMR pulse sequence does not decouple the C-13 nuclei. This leads to erroneous assignments as the C-13 coupled H-1 peaks from the repeating units have similar integrations to those of the H-1 peaks from the terminal groups. Taking into account this coupling allows for more accurate determination of both the molecular weight of the polymer and the efficacy of conjugation of a terminal moiety compared to using the uncoupled H-1 peaks from the repeating unit.
Article
Chemistry, Multidisciplinary
Jacqueline L. Pasek-Allen, Randall K. Wilharm, John C. Bischof, Valerie C. Pierre
Summary: The molecular weight, purity, and functionalization of polyethylene glycols are commonly determined using H-1 NMR spectroscopy. It is important to consider the C-13 coupling in the H-1 NMR pulse sequence for large polymers, as the integrations of the 13C-coupled H-1 peaks from the repeating units can be similar to those of the H-1 peaks from the terminal groups. Ignoring this coupling can lead to incorrect assignments. Once appropriately assigned, these C-13-coupled H-1 peaks can provide more accurate measurements of the polymer's molecular weight and the efficacy of conjugation of a terminal moiety than the uncoupled H-1 peaks from the repeating units.
Review
Oncology
Jen-Fu Hsu, Shih-Ming Chu, Chen-Chu Liao, Chao-Jan Wang, Yi-Shan Wang, Mei-Yin Lai, Hsiao-Chin Wang, Hsuan-Rong Huang, Ming-Horng Tsai
Summary: Glioblastoma multiforme (GBM) is the most common and malignant brain tumor with poor prognosis. The presence of GBM stem cells and the blood brain barrier (BBB) compromise chemotherapy and radiation therapy. Nanotechnology and nanocarrier-based drug delivery offer new possibilities for overcoming GBM treatment resistance.
Article
Chemistry, Medicinal
Masato Maruyama, Haruka Tojo, Keita Toi, Yusuke Ienaka, Kenji Hyodo, Hiroshi Kikuchi, Ken-ichi Ogawara, Kazutaka Higaki
Summary: The effect of doxorubicin release rates from PEG-liposomes on anti-tumor activity was investigated. The study showed that liposomes with a moderate release rate exhibited the highest anti-tumor activity, which was correlated with the distribution of the drug in cancer cells.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Julia Penas-Martinez, Gines Luengo-Gil, Salvador Espin, Nataliya Bohdan, Carmen Ortega-Sabater, Maria Carmen Rodenas, David Zaragoza-Huesca, Maria Jose Lopez-Andreo, Carme Plasencia, Vicente Vicente, Alberto Carmona-Bayonas, Irene Martinez-Martinez
Summary: Our study reveals the versatile anti-tumor properties of prelatent antithrombin on U-87 MG glioblastoma multiforme cells, inhibiting migration and invasion while having a lesser effect on proliferation. Prelatent antithrombin down-regulated the expression of VEGFA, pSTAT3, and pERK1/2 in U-87 MG cells.
Article
Medicine, Research & Experimental
Tien-Ching Chang, Bing-Mae Chen, Jer-Yuan Wu, Tian-Lu Cheng, Steve Roffler
Summary: Antibodies that bind polyethylene glycol (PEG) can accelerate the clearance of pegylated medicines, with higher affinity antibodies leading to greater clearance and reduced drug efficacy. The molar ratio of high affinity anti-PEG antibodies to PEG-EPO correlates with accelerated blood clearance in mice.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Engineering, Biomedical
Shuhan Liu, Ji Liu, Haisong Li, Kuirong Mao, Haorui Wang, Xiandi Meng, Jialiang Wang, Chenxi Wu, Hongmei Chen, Xin Wang, Xiuxiu Cong, Yue Hou, Ye Wang, Ming Wang, Yong-Guang Yang, Tianmeng Sun
Summary: This study develops a novel cationic lipid nanoparticle (LNP) that efficiently delivers siRNA across the blood-brain barrier (BBB) and targets the tumor microenvironment in the brain of mice for immunotherapy of glioblastoma multiforme (GBM). The LNP is effective in delivering siRNA against CD47 and PD-L1 into the cranial GBM in mice, resulting in downregulation of target gene expression and activation of antitumor immunity.
Article
Biochemistry & Molecular Biology
Kittisak Jantanasakulwong, Sarinthip Thanakkasaranee, Phisit Seesuriyachan, Pisith Singjai, Aphisit Saenjaiban, Siriphan Photphroet, Kanticha Pratinthong, Yuthana Phimolsiripol, Noppol Leksawasdi, Thanongsak Chaiyaso, Sarana Rose Sommano, Pensak Jantrawut, Siriwadee Chomdej, Suwit Chotinan, Francisco J. Barba, Joe M. Regenstein, Alissara Reungsang, Pornchai Rachtanapun
Summary: The nano-metal-treated PET films with anti-virus and anti-fogging ability were developed using sparking nano-metal particles of Ag, Zn, and Ti wires on polyethylene terephthalate (PET) films. A highly transparent, anti-fogging, and anti-virus face shield was prepared using the Zn-PET film. Sparking was an effective technique to prepare the alternative anti-virus and anti-fogging films for medical biomaterial applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Zefeng Chen, Yaoxun Zeng, Niping Chen, Mingxia Zhang, Yakun Wang, Zhenxing Pan, Jiongpeng Yuan, Zhaoyi Ye, Xiaojing Li, Wangqing Bian, Haihong Li, Kun Zhang, Yan He, Xujie Liu
Summary: This study proposes a novel approach for the transport of metal ions using polyethylene glycol. By a simple synthesis method, 31 different metal ion-based nanoparticles were successfully synthesized and demonstrated their potential for cancer theranostics.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Pharmacology & Pharmacy
Ben Newland, Johannes Starke, Chiara Bastiancich, Diana P. N. Goncalves, Laura J. Bray, Wenxin Wang, Carsten Werner
Summary: Local drug delivery system can achieve high concentration of therapeutic agents at tumor site, minimizing systemic toxicity. This study developed a well-defined microscale hydrogel incorporating gold nanorods for photothermal therapy. The hydrogel could be loaded with doxorubicin and released it over one week, exhibiting toxicity against glioma cells. Research showed potential for antitumor activity through combining photothermal therapy and drug release.
Article
Dentistry, Oral Surgery & Medicine
Chiao-Wen Lin, Yi-Tzu Chen, Hsieh-Hsun Ho, Pei-Shan Hsieh, Yi-Wei Kuo, Jia-Hung Lin, Cheng-Ruei Liu, Yu-Fen Huang, Ching-Wei Chen, Chen-Hung Hsu, Wen-Yang Lin, Shun-Fa Yang
Summary: The study showed that oral probiotic lozenges significantly increased the population of Lactobacillus in the oral cavity and exhibited antibacterial activity within two weeks. IgA levels, Lactobacillus and Bifidobacterium abundances in the oral cavity were significantly increased in the experimental groups, leading to reduced plaque formation. Most participants experienced improved oral health and intestinal symptoms.
Editorial Material
Otorhinolaryngology
Pei-Hsuan Wu, Chia-Yi Lee, Jing-Yang Huang, Shun-Fa Yang, Cheng-Ping Shih
Summary: Low gestational age, low birth weight, and a long disease course are significantly correlated with late-onset SNHL in patients with cCMV infection.
CLINICAL OTOLARYNGOLOGY
(2022)
Article
Ophthalmology
Chia-Chen Lin, Chia-Yi Lee, Jing-Yang Huang, Sheng-Min Hsu, Jia-Horung Hung, Shun-Fa Yang
Summary: This study provides an in-depth analysis of the trends in DR, DME, and related comorbidities in patients with T2DM. It shows that the prevalence and incidence of DR and PDR decreased over time, while the prevalence and incidence of DME increased. Additionally, the percentage of patients with hyperlipidaemia increased in all forms of DR, while the percentage of patients with ESRD increased only in DME.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kang-Hsi Wu, Ju-Pi Li, Wan-Ru Chao, Yi-Ju Lee, Shun-Fa Yang, Ching-Chang Cheng, Yu-Hua Chao
Summary: The study found that human umbilical cord-derived MSCs effectively attenuated the cytokine storm and lung injury in mice after LPS-induced ALI by down-regulating the MyD88-NFκB signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Yia-Ting Li, Yao-Chen Wang, Shun-Fa Yang, Yat-Yin Law, Bei-Hao Shiu, Te-An Chen, Shih-Chi Wu, Min-Chi Lu
Summary: This study aimed to identify the risk factors of invasive Candida infection (ICI) in surgical critically ill peptic ulcer patients. The results indicated that blood lactate level measured at hospital admission could be a predictor of ICI, and patients with liver cirrhosis and higher Sequential Organ Failure Assessment (SOFA) score had poor outcomes.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Pediatrics
Yu-Jung Chang, Grace Hao, Anpin Ni, Thomas Layton, Jing-Yang Huang, Shun-Fa Yang, Shiuan-Chih Chen
Summary: This study aims to evaluate guidelines for initiating oral feeding in preterm infants and finds that using the Taiwan version of Preterm Oral Feeding Readiness Assessment Scale (TW-POFRAS) in clinical decision-making can help preterm infants to start oral feeding at the appropriate time. For most preterm infants, they can smoothly consume at least 5 mL of milk in one meal and proceed to consume a full day of meals within a week. The average length of stay in the hospital is typically one month, except for infants with a birth weight less than 1,500 g or a gestational age less than 32 weeks.
PEDIATRICS AND NEONATOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Ping-Ju Chen, Hsueh-Ju Lu, Yasser Nassef, Chiao-Wen Lin, Chun-Yi Chuang, Chia-Yi Lee, Yu-Wei Chiu, Shun-Fa Yang, Wei-En Yang
Summary: This study evaluated the associations between dipeptidyl peptidase IV (DPP4) single-nucleotide polymorphism (SNP) and oral cancer. The results showed that DPP4 SNP rs2268889 may correlate to the development of oral cancer in those with cigarette smoking and alcohol consumption. It was also found that this SNP may relate to worse clinical course of oral cancer in non-smokers.
JOURNAL OF ORAL PATHOLOGY & MEDICINE
(2022)
Article
Oncology
Hsueh-Ju Lu, Chih-Yu Peng, Hsien-Chun Tseng, Chung-Han Hsin, Chun-Yi Chuang, Chun-Chia Chen, Wei-Shiou Huang, Yu-Wei Chiu, Shun-Fa Yang
Summary: This study retrospectively analyzed 262 patients with OCSCC and divided them into resectable and unresectable groups. The study found that salvage surgery had an impact on the survival of OCSCC patients and established a scoring model to predict life expectancy after salvage surgery.
Article
Multidisciplinary Sciences
Shih-Ching Lo, Kevin Sheng-Kai Ma, Yen-Ru Li, Zi-Yue Li, Cheng-Hung Lin, Hsing-Chun Lin, Shun-Fa Yang
Summary: There is an association between nutritional provision and successful weaning from ventilators, suggesting that adequate nutrition support may increase the chances of successful extubation in patients.
SCIENTIFIC REPORTS
(2022)
Article
Environmental Sciences
Chung-Yuan Lee, Yung-Chuan Ho, Chiao-Wen Lin, Min-Chieh Hsin, Po-Hui Wang, Ya-Cheng Tang, Shun-Fa Yang, Yi-Hsuan Hsiao
Summary: The synthetic curcumin analog, EF-24, has been shown to have enhanced bioavailability and stronger anti-inflammatory and anti-cancer activity compared to curcumin. This study investigated the effects of EF-24 on TPA-induced cellular migration of cervical cancer and found that EF-24 significantly reduced cellular migration and invasion. Further analysis revealed that EF-24 modulated MMP-9 expression by inhibiting the phosphorylation of the p38 signaling pathway. These findings suggest that EF-24 may have potential as a chemopreventive agent for cervical cancer.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Ophthalmology
Hsiang-Wen Chien, Chia-Yi Lee, Jing-Yang Huang, Hung-Chi Chen, Kai Wang, Shun-Fa Yang
Summary: This study, using the National Health Insurance Research Database of Taiwan, found a relationship between congenital cytomegalovirus (CMV) infection and late-onset keratitis. Severe congenital CMV infection was found to be significantly correlated with late-onset keratitis in individuals with congenital CMV infection.
EUROPEAN JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ruei-Hong Lin, Hsu-Tung Lee, Chun-An Yeh, Yi-Chin Yang, Chiung-Chyi Shen, Kai-Bo Chang, Bai-Shuan Liu, Hsien-Hsu Hsieh, Hui-Min David Wang, Huey-Shan Hung
Summary: The research shows that using AuNPs at concentrations of 1.25 and 2.5 ppm can enhance cell proliferation, reduce reactive oxygen species generation, and attenuate platelet/monocyte activation. AuNPs can also promote cell adhesion, increase the expression of matrix metalloproteinase (MMP-2/9), decrease cell apoptosis and the release of inflammatory cytokines. Animal model experiments demonstrate that treating with 1.25 and 2.5 ppm AuNPs exhibits better biocompatibility and endothelialization. This suggests that AuNPs can be used as a biocompatible nanomedicine for regeneration research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Wen-Yu Cheng, Meng-Yin Yang, Chun-An Yeh, Yi-Chin Yang, Kai-Bo Chang, Kai-Yuan Chen, Szu-Yuan Liu, Chien-Lun Tang, Chiung-Chyi Shen, Huey-Shan Hung
Summary: This study investigated the biocompatibility, biological performances, and cell uptake efficiency of AuNP in Wharton's jelly mesenchymal stem cells and a rat model. Various concentrations of AuNP were prepared and characterized. In vitro examinations showed that treatment with 1.25 and 2.5 ppm AuNP improved viability, CXCR4 expression, migration distance, and reduced apoptotic-related protein expression in Wharton's jelly MSCs. AuNP-Col was taken up by cells via clathrin-mediated endocytosis and the vacuolar-type H+-ATPase pathway. In vivo examinations demonstrated that 2.5 ppm AuNP attenuated foreign body responses and had better retention efficacy in tissue integrity. Overall, AuNP shows promise as a biosafe nanodrug delivery system for regenerative medicine with Wharton's jelly MSCs.
Article
Cell Biology
Meng-Yin Yang, Cheng-Di Chiu, Yi-Chun Ke, Yi-Chin Yang, Kai-Bo Chang, Chien-Min Chen, Hsu-Tung Lee, Chien-Lun Tang, Bai-Shuan Liu, Huey-Shan Hung
Summary: Au decorated with type I collagen (Col) was used as a core material to cross-link with stromal cell-derived factor 1 alpha (SDF1 alpha) for investigating its biological performance. The physicochemical characteristics of the Au-based nanoparticles were determined using SEM, DLS, UV-Vis, and FTIR. The biocompatibility of the nanoparticles was evaluated using MSCs, and their potential for inducing cell differentiation and transportation in cells was explored.
Article
Medicine, General & Internal
Kwei-Jing Chen, Ming-Hong Hsieh, Yen-You Lin, Michael Yuan-Chien Chen, Ming-Yu Lien, Shun-Fa Yang, Chih-Hsin Tang
Summary: Oral cancer is a common cancer in Taiwanese males, and elevated levels of visfatin, a pro-inflammatory adipocytokine, have been found in oral squamous cell carcinoma (OSCC) patients. This study investigated the effects of visfatin gene polymorphisms and carcinogenic lifestyle factors on the risk of developing OSCC, as well as their associations with prognostic factors. The results show that certain visfatin gene polymorphisms and lifestyle factors may be associated with the development and prognosis of OSCC.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2022)