Review
Genetics & Heredity
Ravindra Prasad
Summary: Wheat is an important cereal crop consumed by a large population, but it is threatened by spot blotch disease and nutrient deficiencies. Cytokinins, a type of plant hormone, play a crucial role in plant growth, stress response, and nutrient metabolism. Increasing micronutrient accumulation and improving crop resilience through crop bio-fortification are vital for improving wheat crops.
FRONTIERS IN GENETICS
(2022)
Review
Genetics & Heredity
Lekshmy Sathee, B. Jagadhesan, Pratheek H. Pandesha, Dipankar Barman, Sandeep B. Adavi, Shivani Nagar, G. K. Krishna, Shailesh Tripathi, Shailendra K. Jha, Viswanathan Chinnusamy
Summary: This review discusses the development of RNA-guided genome editing technology (CRISPR-Cas9) and its potential applications in improving nutrient use efficiency and stress tolerance in plants. It outlines different targets for genome editing and strategies for enhancing nutrient uptake and stress signaling. The use of CRISPR/dCas9 system also allows for targeted overexpression of genes of interest and DNA methylation in plants.
FRONTIERS IN GENETICS
(2022)
Review
Genetics & Heredity
Gauri Nerkar, Suman Devarumath, Madhavi Purankar, Atul Kumar, R. Valarmathi, Rachayya Devarumath, C. Appunu
Summary: The challenges posed by global climate change and various unfavorable factors in agriculture have led to the emergence of genome editing technology, which provides a new approach to improving crop productivity. The technology enables precise modification of crop genomes, accelerating the breeding process and enhancing efficiency.
FRONTIERS IN GENETICS
(2022)
Review
Plant Sciences
Arya Bagus Boedi Iswanto, Rahul Mahadev Shelake, Minh Huy Vu, Jae-Yean Kim, Sang Hee Kim
Summary: Plasmodesmata play a crucial role in intercellular communication and molecular exchange in plant cells, with potential applications in crop improvement. Researchers suggest that genome editing techniques like CRISPR/Cas offer powerful tools to analyze the function of plasmodesmata components and engineer elite crops.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Manish Roorkiwal, Aditi Bhandari, Rutwik Barmukh, Prasad Bajaj, Vinod Kumar Valluri, Annapurna Chitikineni, Sarita Pandey, Bharadwaj Chellapilla, Kadambot H. M. Siddique, Rajeev K. Varshney
Summary: Micronutrient malnutrition is a global concern, and this study focused on enhancing the nutrient content of chickpea. By evaluating a diverse set of chickpea germplasm, the study identified significant variations in nutritional traits and conducted genome-wide association studies to identify marker-trait associations. The study also explored the effect of heterozygosity on trait detection. Seven promising accessions were identified as potential donors for developing nutrient-rich, high-yielding chickpea varieties.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Genetics & Heredity
Dileep Kumar, Anurag Yadav, Rumana Ahmad, Upendra Nath Dwivedi, Kusum Yadav
Summary: The global malnutrition burden has long-term consequences for individuals, communities, and countries. Biotechnology has introduced biofortification to combat malnutrition, but current methods are not sustainable. The CRISPR-Cas-based system of genome editing offers a targeted and ethical solution to these limitations. This system has been extensively used in crop improvement programs, enhancing nutritional value and resistance.
FRONTIERS IN GENETICS
(2022)
Article
Plant Sciences
Praveen Awasthi, Shahirina Khan, Hiralben Lakhani, Siddhant Chaturvedi, Shivani, Navneet Kaur, Jagdeep Singh, Atul Kumar Kesarwani, Siddharth Tiwari
Summary: Carotenoid cleavage dioxygenases (CCDs) play an important role in the metabolism of carotenoids in plants. In this study, we investigated the regulatory mechanism of beta-carotene homeostasis in banana using transgene-free genome editing. We found that CCD4 is a key regulator of beta-carotene degradation and its expression is correlated with carotenoid concentrations. Additionally, our study demonstrated the successful use of genome editing in increasing beta-carotene accumulation in biofortified banana lines.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Plant Sciences
Bhupendra Koul, Komal Sharma, Vrinda Sehgal, Dhananjay Yadav, Meerambika Mishra, Chellapilla Bharadwaj
Summary: Chickpea, the world's second most consumed legume crop, is rich in nutrients and beneficial for diabetics. With the increasing world population, chickpea production should be increased to meet the demand. Utilizing genomic technologies in breeding is crucial for developing high yielding varieties to combat abiotic and biotic stresses.
Article
Multidisciplinary Sciences
Noman Mehboob, Muhammad Rizwan, Waqas Ahmed Minhas, Tauqeer Ahmad Yasir, Amal M. Al-Mohaimeed, Mohamed S. Elshikh, Mubshar Hussain
Summary: The study evaluated the response of different 'desi' and 'kabuli' chickpea genotypes to boron application, finding that under 1 kg/ha boron application, the 'Punjab-2008' and 'NOOR-2009' genotypes exhibited the highest growth and yield characteristics, making them preferred genotypes for producing boron-rich grains.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Plant Sciences
Tamanna A. Jahan, Shweta Kalve, Zachery Belak, Christopher Eskiw, Bunyamin Tar'an
Summary: Chickpea is a staple food in developing countries and can help alleviate iron deficiency. This study investigated iron accumulation and gene expression in different organs of chickpea plants at various growth stages. The highest iron accumulation was found in roots, while gene expression analysis revealed the involvement of certain genes in iron uptake and metabolism. This knowledge can be applied to develop chickpea varieties with high iron content in seeds.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Xiao Sun, Liming Zhu, Zhaodong Hao, Weihuang Wu, Lin Xu, Yun Yang, Jiaji Zhang, Ye Lu, Jisen Shi, Jinhui Chen
Summary: Liriodendron chinense, a tree species used for landscaping and timber production, is affected by temperature and drought stress. This study identified 5 genes responsible for regulating cytokinin levels in L. chinense, and found that these genes respond to environmental stress. These findings provide a foundation for breeding resistant varieties of this rare and endangered tree species.
Review
Biology
Alpana Joshi, Seo-Yeon Yang, Hyung-Geun Song, Jiho Min, Ji-Hoon Lee
Summary: Plants are affected by various environmental stresses, which have a negative impact on their growth and development and limit crop productivity. Developing cultivars resistant to abiotic stresses is essential to meet the needs of the growing world population and ensure food security. With the availability of genetic databases and advancements in genome editing techniques, it is now possible to edit target genes with precision and create new opportunities for crop improvement. The CRISPR-Cas system is a powerful genome editing method that allows the development of improved cultivars with resistance to abiotic stresses. This review discusses the current advances in understanding the molecular mechanisms of abiotic stress responses in plants and the application of CRISPR-Cas system genome editing technology for developing crop resilience against drought, salinity, temperature, heavy metals, and herbicides.
Review
Genetics & Heredity
Charul Singh, Ramesh Kumar, Hansa Sehgal, Sharmista Bhati, Tripti Singhal, Gayacharan, M. S. Nimmy, Renu Yadav, Santosh Kumar A. Gupta, Naglaa Abdallah, Aladdin Hamwieh, Rajendra Kumar
Summary: Genomics and genome editing offer great opportunities for crop improvement and fundamental research. The emergence of new genome editing techniques such as zinc finger nucleases (ZFNs), homing endonucleases, transcription activator-like effector nucleases (TALENs), Base Editors (BEs), and Primer Editors (PEs) enables scientists to modulate gene expressions or create novel genes with high precision and efficiency. However, these techniques are expensive and laborious, requiring protein engineering as a prerequisite. Unlike first-generation genome editing methods, CRISPR/Cas9 is easy to construct and can theoretically target multiple locations in the genome with different guide RNAs. This study discusses the progress in genome editing tools, their applications in chickpea crop development, scientific limitations, and future perspectives for enhancing drought resistance, heat tolerance, and yield in chickpea to address global climate change, hunger, and nutritional threats.
FRONTIERS IN GENETICS
(2023)
Review
Agronomy
Sakthivel Surya Krishna, S. R. Harish Chandar, Maruthachalam Ravi, Ramanathan Valarmathi, Kasirajan Lakshmi, Perumal Thirugnanasambandam Prathima, Ramaswamy Manimekalai, Rasappa Viswanathan, Govindkurup Hemaprabha, Chinnaswamy Appunu
Summary: Sugarcane (Saccharum spp.) is a valuable crop, but its production is limited by various biotic and abiotic stresses. CRISPR/Cas genome editing technology allows the creation of new resistant cultivars, and transgene-free genome editing techniques overcome commercial exploitation limitations. These advancements accelerate the development of climate-smart cultivars with high yields.
Review
Plant Sciences
Jagesh Kumar Tiwari, Anand Kumar Singh, Tusar Kanti Behera
Summary: The narrow genetic base of tomato presents challenges in breeding, but with the development of CRISPR/Cas9 genome editing, fast and efficient breeding has become possible. CRISPR/Cas9 has been used to edit and characterize various traits in tomato, such as plant architecture, flower characters, fruit quality, disease resistance, and stress tolerance. It has also been used to introduce elite traits from wild relatives to cultivated tomatoes. This mini-review highlights the current research progress in using CRISPR/Cas9 for tomato breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Priya Kumari, Vijay Gahlaut, Ekjot Kaur, Sanatsujat Singh, Sanjay Kumar, Vandana Jaiswal
Summary: In this study, a comprehensive exploration of the rose GRAS gene family was performed and their potential role in plant growth, stress, and hormonal response was depicted.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Review
Genetics & Heredity
Rajesh Kumar Singh, Charul Singh, B. S. Ambika, B. S. K. Chandana, Rohit Mahto, Ranjana Patial, Astha Gupta, Vijay Gahlaut, H. D. Gayacharan, Aladdin Hamwieh, H. D. Upadhyaya, Rajendra Kumar
Summary: Legume crops provide significant nutrition and improve cropping environment. However, genetic improvement in legumes is limited, and measures need to be taken to broaden the genetic base and enhance productivity, stress resilience, and nutritional values.
FRONTIERS IN GENETICS
(2022)
Article
Pharmacology & Pharmacy
Anil Pandey, Neelam Yadav
Summary: The current research analyzed the efficacy of lactic acid bacteria (LAB) isolated from traditional dairy products in Prayagraj, India. The mixed strains showed better viability and antimicrobial activity in simulated gastric juice and simulated intestinal juice compared to individual strains. Both the mixed and individual strains were susceptible to most antibiotics, except amphotericin.
ANNALS OF PHYTOMEDICINE-AN INTERNATIONAL JOURNAL
(2022)
Article
Agronomy
Rakhi Singh, Gautam Saripalli, Anuj Kumar, Tinku Gautam, Susheel Kumar Singh, Vijay Gahlaut, Sachin Kumar, Prabina Kumar Meher, Rajendra Prasad Mishra, Vinod Kumar Singh, Pradeep Kumar Sharma, Harindra Singh Balyan, Pushpendra Kumar Gupta
Summary: This study investigated the genetic basis of nitrogen use efficiency (NUE) and its component traits NUpE and NUtE in wheat. A bi-parental RIL mapping population, along with parental and check genotypes, were evaluated under different nitrogen levels for three years. A genetic map with 456 SNP markers was used for QTL analysis and 36 QTLs for NUE, NUpE, and NUtE were identified. Candidate genes involved in metabolic and cellular processes were found in the QTL regions, some of which showed differential expression in roots and shoots. These findings contribute to the understanding of NUE and provide potential targets for molecular-assisted selection (MAS) in wheat improvement.
Article
Food Science & Technology
Vandana Verma, Vijayta Singh, Om Prakash Chauhan, Neelam Yadav
Summary: This study investigated the use of different frying methods, including deep fat frying, microwave frying, air frying, and vacuum frying, to reduce the formation of acrylamide (AA) and hydroxymethylfurfural (HMF) in French fries. The highest reduction in AA and HMF content was observed in vacuum frying (61.42-81.14%), followed by air frying (59.2-78.7%), compared to deep fat frying and microwave frying. Samples fried using these methods also had significantly lower browning index (BI) and CIE a* values. Principal component analysis (PCA) demonstrated that vacuum frying and air frying were alternative technologies for minimizing AA and HMF content in French fries.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2023)
Review
Genetics & Heredity
Astha Gupta, Tripti Sharma, Surendra Pratap Singh, Archana Bhardwaj, Deepti Srivastava, Rajendra Kumar
Summary: Microgreens are nutrient-dense superfoods with multiple health benefits such as anti-microbial, anti-inflammatory, antioxidant, and anti-carcinogenic properties. They can be used as plant-based vegetarian foods for garnishing and enhancing flavor and texture. Integrated approaches involving genomics, transcriptomics, molecular breeding, and machine learning can be utilized to breed microgreens with increased nutritional content and improved traits.
FRONTIERS IN GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Himanshi Gangwar, Vijay Gahlaut, Ramesh Chauhan, Satbeer Singh, Vandana Jaiswal
Summary: This study developed a molecular marker that can distinguish between true cinnamon and cassia cinnamon. A total of 71 ISSR markers and four universal barcoding genes were used for identification. The ISSR-37 marker showed a clear distinction between the two species and produced different sized amplicons. These polymorphic bands were converted into species-specific SCAR markers. The SCAR-CV marker was specific to true cinnamon and did not amplify in cassia cinnamon samples. The SCAR marker generated in this study can be used as an efficient, economical, and reliable molecular tool for the identification of true cinnamon.
MOLECULAR BIOLOGY REPORTS
(2023)
Review
Plant Sciences
Vikas Mangal, Milan Kumar Lal, Rahul Kumar Tiwari, Muhammad Ahsan Altaf, Salej Sood, Vijay Gahlaut, Ashish Bhatt, Ajay Kumar Thakur, Ravinder Kumar, Vinay Bhardwaj, Vinod Kumar, Brajesh Singh, Rajender Singh, Devendra Kumar
Summary: Abiotic stresses have a negative impact on vegetable crops, but research on crop genomes can help identify stress-responsive genes. Understanding omics approaches and advanced molecular tools is crucial for studying the biology of abiotic stresses.
Article
Plant Sciences
Komal Goel, Pravesh Kundu, Vijay Gahlaut, Paras Sharma, Ayush Kumar, Shiwali Thakur, Vipasha Verma, Bhavya Bhargava, Rahul Chandora, Gaurav Zinta
Summary: Two major challenges for the future are global temperature increase and population growth, which threaten agricultural productivity and food security. Underutilized crops, such as grain amaranths, have the potential to be future climate crops due to their resilience to climate and nutritional quality. However, the heat tolerance of grain amaranths is still unknown.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Genetics & Heredity
Charul Singh, Ramesh Kumar, Hansa Sehgal, Sharmista Bhati, Tripti Singhal, Gayacharan, M. S. Nimmy, Renu Yadav, Santosh Kumar A. Gupta, Naglaa Abdallah, Aladdin Hamwieh, Rajendra Kumar
Summary: Genomics and genome editing offer great opportunities for crop improvement and fundamental research. The emergence of new genome editing techniques such as zinc finger nucleases (ZFNs), homing endonucleases, transcription activator-like effector nucleases (TALENs), Base Editors (BEs), and Primer Editors (PEs) enables scientists to modulate gene expressions or create novel genes with high precision and efficiency. However, these techniques are expensive and laborious, requiring protein engineering as a prerequisite. Unlike first-generation genome editing methods, CRISPR/Cas9 is easy to construct and can theoretically target multiple locations in the genome with different guide RNAs. This study discusses the progress in genome editing tools, their applications in chickpea crop development, scientific limitations, and future perspectives for enhancing drought resistance, heat tolerance, and yield in chickpea to address global climate change, hunger, and nutritional threats.
FRONTIERS IN GENETICS
(2023)
Editorial Material
Genetics & Heredity
Abhishek Bohra, Vijay Gahlaut, Dragan Perovic, Rajeev K. Varshney
FRONTIERS IN GENETICS
(2023)
Article
Genetics & Heredity
Jyoti Chaudhary, Tinku Gautam, Vijay Gahlaut, Kalpana Singh, Sourabh Kumar, Ritu Batra, Pushpendra Kumar Gupta
Summary: This study identified nine wheat RuvBL genes that are involved in providing tolerance against abiotic stresses. These genes showed synteny with corresponding genes in related species and were associated with regulatory cis-elements and transposable elements. They were closely associated with ATP-dependent formation of histone acetyltransferase complex.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Agronomy
Deepali Upadhyay, Neeraj Budhlakoti, D. C. Mishra, Jyoti Kumari, Vijay Gahlaut, Nidhee Chaudhary, Jasdeep Chatrath Padaria, Sindhu Sareen, Sundeep Kumar
Summary: Abiotic stresses, such as drought, have a significant impact on wheat production, causing up to 86% yield losses. This study evaluated the tolerance of 72 diverse wheat genotypes to drought stress and identified two tolerant genotypes (IC36761A and IC128335) with promising physio-biochemical traits. These genotypes exhibited higher membrane stability index, relative water content, and lower levels of tannin under drought conditions. The findings suggest that IC36761A and IC128335 have the potential to be included in breeding programs aimed at enhancing drought tolerance in bread wheat.
GENETIC RESOURCES AND CROP EVOLUTION
(2023)
Article
Plant Sciences
Surbhi Mali, Madhushree Dutta, Komal Goel, Vidhi Raturi, Pravesh Kundu, Ravi Kumar, Vijay Gahlaut, Vishal Acharya, Vijai Kishor Gupta, Salej Sood, Gaurav Zinta
Summary: Potato is an important food crop that is rich in starch, proteins, antioxidants, and vitamins. However, it is negatively affected by temperature rise. In this study, 26 StJMJ proteins were identified in potato, and they were classified into five different classes. Gene expression analysis showed that the StJMJ genes are heat-inducible and play an important role in modulating heat tolerance in potato.
JOURNAL OF PLANT GROWTH REGULATION
(2023)