Article
Chemistry, Multidisciplinary
Martina M. De Santis, Hani N. Alsafadi, Sinem Tas, Deniz A. Bolukbas, Sujeethkumar Prithiviraj, Iran A. N. Da Silva, Margareta Mittendorfer, Chiharu Ota, John Stegmayr, Fatima Daoud, Melanie Koenigshoff, Karl Sward, Jeffery A. Wood, Manlio Tassieri, Paul E. Bourgine, Sandra Lindstedt, Sofie Mohlin, Darcy E. Wagner
Summary: The study introduces a hybrid bioink composed of alginate and rECM for 3D bioprinting, showing promising printing properties and biological inductive capabilities for generating human tissue.
ADVANCED MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Bram G. Soliman, Alessia Longoni, Mian Wang, Wanlu Li, Paulina N. Bernal, Alessandro Cianciosi, Gabriella C. J. Lindberg, Jos Malda, Juergen Groll, Tomasz Jungst, Riccardo Levato, Jelena Rnjak-Kovacina, Tim B. F. Woodfield, Yu Shrike Zhang, Khoon S. Lim
Summary: Sacrificial printing involves using 3D-printed sacrificial ink to create open-channels in hydrogel constructs. Traditional sacrificial inks lack the ability to mimic tissue development dynamics. To overcome this, a new class of sacrificial inks was developed with tailorable and programmable delayed dissolution profiles. These inks showed compatibility with various biofabrication technologies and allowed precise control over the introduction of architectural features into cell-laden hydrogel constructs.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Biomedical
Giuseppe Guagliano, Cristina Volpini, Jacopo Camilletti, Francesca Donnaloja, Francesco Briatico-Vangosa, Livia Visai, Paola Petrini
Summary: Bioprinting is a key technique for fabricating cell-laden constructs with controlled geometry. We implemented an unconventional strategy by optimizing the design of structures to promote high oxygenation, mimicking the characteristics of hepatic tissue. Through computational methods and a combination of a priori and a posteriori analyses, we successfully printed constructs using an internally crosslinked bioink and cultured them for up to 12 days, demonstrating the suitability of Hep3Gel for mid/long-term cultures.
Article
Biochemistry & Molecular Biology
Lukas Hahn, Matthias Beudert, Marcus Gutmann, Larissa Kessler, Philipp Stahlhut, Lena Fischer, Emine Karakaya, Thomas Lorson, Ingo Thievessen, Rainer Detsch, Tessa Luehmann, Robert Luxenhofer
Summary: In this study, a Diels-Alder click chemistry approach was introduced into thermogelling diblock copolymers to achieve printability and cell-friendliness, while functional modifications were made through acid/amine coupling and thiol-maleimide chemistry to facilitate cell adhesion. Testing showed good biocompatibility of the bioink formulations, which combined physical precursor gelation with additional chemical stabilization for versatile biomechanical adaptation.
MACROMOLECULAR BIOSCIENCE
(2021)
Review
Polymer Science
Larisa T. Volova, Gennadiy P. Kotelnikov, Igor Shishkovsky, Dmitriy B. Volov, Natalya Ossina, Nikolay A. Ryabov, Aleksey V. Komyagin, Yeon Ho Kim, Denis G. Alekseev
Summary: The musculoskeletal system is crucial to the human body, but various factors can damage its elements, especially articular cartilage. Traditional treatment methods are ineffective in repairing articular cartilage, but biofabrication technologies, such as three-dimensional bioprinting, offer new treatment approaches. This review summarizes the main achievements in articular cartilage biofabrication, including technological processes and necessary biomaterials, cell cultures, and signal molecules.
Article
Biochemistry & Molecular Biology
Hitendra Kumar, Kabilan Sakthivel, Mohamed G. A. Mohamed, Emilie Boras, Su Ryon Shin, Keekyoung Kim
Summary: Optimized synthesis parameters were used to develop GelMA bioinks with slow sol-gel transition at room temperature and visible light crosslinkable functions. Eight GelMA combinations suitable for DLP-SLA bioprinting were identified through systematic physical and rheological property characterizations. RO-GelMA bioinks were found to be most suitable for achieving high resolution in DLP-SLA printing, showing excellent biocompatibility and cell survival rates.
MACROMOLECULAR BIOSCIENCE
(2021)
Review
Biotechnology & Applied Microbiology
Sam P. Tarassoli, Zita M. Jessop, Thomas Jovic, Karl Hawkins, Iain S. Whitaker
Summary: Bioprinting is increasingly using natural biomaterials and there is no established tissue specific bioinks or bioprinting techniques. Further research and standardization of biomechanical assessment types and timing are needed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Sarah M. Hull, Lucia G. Brunel, Sarah C. Heilshorn
Summary: Using gel-phase materials as bioinks offers advantages in providing cell protection and biological signals. Challenges remain in terms of cell compatibility and biological functionality of bioink materials.
ADVANCED MATERIALS
(2022)
Review
Engineering, Biomedical
Yanli Cai, Soon Yee Chang, Soo Wah Gan, Sha Ma, Wen Feng Lu, Ching-Chiuan Yen
Summary: This review paper focuses on the recent developments in cell-laden nanocomposite bioinks for 3D bioprinting and their effects on the properties and performance of the bioinks. The reinforcement mechanisms and interactions between cells and nano-materials are discussed, with an emphasis on bone and cartilage tissue engineering applications. The limitations and future prospects of current cell-laden nanocomposite bioinks are also addressed.
ACTA BIOMATERIALIA
(2022)
Review
Engineering, Biomedical
Qian Wang, Yang Liu, Shuqing Zhang, Fan He, Tong Shi, Jizong Li, Zhimin Wang, Jia Jia
Summary: Bioprinting is a promising technology for tissue engineering and regenerative medicine, but one of its limitations is the reproduction of the extracellular matrix (ECM) with sufficient bioactivities for bioprinted cells. Exosomes, natural biological particles, show great potential in supporting cells for bioprinting. This review summarizes the recent advances of exosome-based bioprinting in tissue engineering and regenerative medicine, including applications in bone tissue engineering, immunological regulations, angiogenesis, and neural regenerations.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Review
Engineering, Biomedical
Ming Li, Xueer Song, Sha Jin, Kaiming Ye
Summary: This review highlights the use of three-dimensional tumor models in cancer pathology studies and drug discovery, which can simulate the complex human tumor microenvironment. Various biofabrication technologies, including 3D bioprinting techniques, are discussed for characterizing tumor progression and response to treatment.
BIO-DESIGN AND MANUFACTURING
(2021)
Review
Polymer Science
Yeong-Jin Choi, Honghyun Park, Dong-Heon Ha, Hui-Suk Yun, Hee-Gyeong Yi, Hyungseok Lee
Summary: This paper provides an in-depth assessment of the recently developed 3D bioprinting technology and bioinks, focusing on the latest achievements and future aspects of using 3D bioprinting for in vitro modeling.
Review
Biochemistry & Molecular Biology
Ethan Hau Yin Lam, Fengqing Yu, Sabrina Zhu, Zongjie Wang
Summary: In the past decade, personalized medicine has made significant progress in addressing patient-specific disease complexities and developing individualized treatment strategies. The emergence of 3D bioprinting has provided novel opportunities for personalized medicine, although current bioprinted constructs have limitations in achieving anatomically realistic organs with mature biological functions. This review discusses the principles and realizations of bioprinting, focusing on techniques such as extrusion printing and digital light processing (DLP). It also explores the applications of bioprinted constructs in regenerative medicine and drug discovery. Despite the challenges, bioprinting shows promise as an empowering technology to overcome critical obstacles in personalized medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Przemys law Gnatowski, Edyta Pilat, Justyna Kucinska-Lipka, Mohammad Reza Saeb, Michael R. Hamblin, Masoud Mozafari
Summary: Cancerous tumors are highly fatal, claiming almost 10 million lives in 2020 worldwide. Accurately modeling tumors is a challenging task due to their complex and dynamic nature. Hydrogel-based 3D bioprinting is emerging as a promising approach to overcome the limitations of current models and mimic cancer development and behavior.
TRANSLATIONAL ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Annika Lechner, Vanessa T. Trossmann, Thomas Scheibel
Summary: The printability of bioinks refers to considerations such as rheology, extrudability, filament formation, shape fidelity, cell viability, and cellular development post-printing. Recent studies indicate that recombinant spider silk hydrogels show high shape fidelity and may be suitable for bioink formulations, with the encapsulation of cells impacting gelation and mechanics differently depending on the silk variant used. RGD-modified spider silk hydrogels are physically crosslinked by cells, showing high cell viability after extrusion-based printing.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Duyen H. T. Nguyen, Robert H. Utama, Kristel C. Tjandra, Panthipa Suwannakot, Eric Y. Du, Maria Kavallaris, Richard D. Tilley, J. Justin Gooding
Summary: This study develops a synthetic hydrogel with ionic cross-linking to rapidly create hydrogels. The mechanical stiffness of the hydrogel can be tuned by varying the number of charged ionic groups, the length of the polymer arms, and the polymer concentration. It is demonstrated as an extracellular matrix mimic for 3D in vitro cell models.
Article
Chemistry, Physical
Samuel V. Somerville, Peter B. O'Mara, Tania M. Benedetti, Soshan Cheong, Wolfgang Schuhmann, Richard D. Tilley, J. Justin Gooding
Summary: Enzymes with multiple active sites and control over the solution environment enable the formation of complex products from simple reactants. We mimic this concept using nanoparticles to facilitate the electro-chemical carbon dioxide reduction reaction. By altering the rate of CO2 delivery, the activity of the CO producing site, and the applied potential, we show that stable nanoparticles with lower CO formation activity can produce greater amounts of hydrocarbon products. This highlights the importance of the local solution environment and the stability of the catalyst in cascade reactions.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Review
Pharmacology & Pharmacy
Estrella Gonzales-Aloy, Aria Ahmed-Cox, Maria Tsoli, David S. Ziegler, Maria Kavallaris
Summary: Brain cancer is the most deadly cancer and the blood-brain barrier (BBB) poses a challenge for drug delivery. The BBB becomes disrupted in brain cancers, forming the blood-brain tumor barrier (BBTB). 3D cell models have the potential to serve as physiologically relevant in vitro models for studying the BBB and BBTB.
ADVANCED DRUG DELIVERY REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Samuel V. V. Somerville, Qinyu Li, Johanna Wordsworth, Sina Jamali, Mohammad Reza Eskandarian, Richard D. D. Tilley, J. Justin Gooding
Summary: Nanozymes mimic the selectivity of enzymes by utilizing features such as control over the arrangement of atoms in the active site and the placement of the active site down a nanoconfined substrate channel. The implementation of enzyme-inspired features has shown improvements in both activity and selectivity of nanoparticles for various catalytic and sensing applications. Controlled active sites on metal nanoparticle surfaces can be achieved through changing the composition of the surface metal or immobilizing single atoms on a metal substrate. Molecular frameworks and unique diffusional environments further enhance selectivity, while nanoconfined substrate channels offer additional control over selectivity through modifying the solution environment and transport of reactants and products.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Analytical
Danielle Bennett, Xueqian Chen, Gregory J. Walker, Sacha Stelzer-Braid, William D. Rawlinson, D. Brynn Hibbert, Richard D. Tilley, J. Justin Gooding
Summary: Plasmonic nanoparticles in dimer format are used for single molecule sensing, where the interaction with hairpin DNA leads to a shift in localized surface plasmon resonance. Spectroscopy may detect this shift, but point-of-care devices require a faster analysis method. By using dark-field imaging and digital analysis, the plasmonic resonance shift of thousands of dimer structures can be measured in minutes. The challenge is separating dimers from non-specifically bound clusters to achieve accurate results. The LAB-based classifier algorithm demonstrated the highest accuracy for this digital separation.
ANALYTICAL CHEMISTRY
(2023)
Correction
Chemistry, Analytical
Danielle Bennett, Xueqian Chen, Gregory J. Walker, Milad Mehdipour, Sacha Stelzer-Braid, William D. Rawlinson, D. Brynn Hibbert, Richard D. Tilley, J. Justin Gooding
ANALYTICAL CHEMISTRY
(2023)
Editorial Material
Chemistry, Analytical
Eric Bakker, Philippe Buhlmann, J. Justin Gooding, Robert E. Gyurcsanyi, Ernoe Pretsch
Article
Chemistry, Multidisciplinary
Taskeen Iqbal Janjua, Yuxue Cao, Aria Ahmed-Cox, Aun Raza, Md Moniruzzaman, Dewan Taslima Akhter, Nicholas L. Fletcher, Maria Kavallaris, Kristofer J. Thurecht, Amirali Popat
Summary: Despite advancements in neuro-oncology, the prognosis for brain cancers such as glioblastoma remains poor. This study demonstrates that using nanoparticles as drug carriers can improve drug delivery across the blood-brain barrier and enhance the efficacy of glioblastoma therapy.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Cell Biology
Ernest Moles, Christopher B. Howard, Pie Huda, Mawar Karsa, Hannah McCalmont, Kathleen Kimpton, Alastair Duly, Yongjuan Chen, Yizhou Huang, Melinda L. Tursky, David Ma, Sonia Bustamante, Russell Pickford, Patrick Connerty, Sofia Omari, Christopher J. Jolly, Swapna Joshi, Sylvie Shen, John E. Pimanda, Alla Dolnikov, Laurence C. Cheung, Rishi S. Kotecha, Murray D. Norris, Michelle Haber, Charles E. de Bock, Klaartje Somers, Richard B. Lock, Kristofer J. Thurecht, Maria Kavallaris
Summary: High-risk childhood leukemia has a poor prognosis due to treatment failure and toxic side effects. Encapsulation of drugs in liposomal nanocarriers improves biodistribution and tolerability, but lacks selectivity for cancer cells. In this study, bispecific antibodies were generated to target PEGylated liposomal drugs to leukemic cells. These antibodies improved targeting and cytotoxic activity, with minimal harm to normal cells. Targeted delivery using bispecific antibodies enhanced leukemia suppression and extended overall survival, representing a promising platform for improved treatment of high-risk leukemia.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Materials Science, Biomaterials
Panthipa Suwannakot, Stephanie Nemec, Newton Gil Peres, Eric Y. Du, Kristopher A. Kilian, Katharina Gaus, Maria Kavallaris, J. Justin Gooding
Summary: Synthetic hydrogels are widely used to mimic the extracellular matrix (ECM) and the physical and biochemical cues observed in natural ECM proteins. Researchers have developed an electrostatically crosslinked PEG-based hydrogel system to create high-throughput 3D in vitro models of the cancer environment. This hydrogel system can be degraded by breaking the interaction between oppositely charged polymer chains.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biophysics
Ying Yang, Sanjun Fan, James A. Webb, Yuanqing Ma, Jesse Goyette, Xueqian Chen, Katharina Gaus, Richard D. Tilley, Justin Gooding
Summary: This study presents an electrochemical approach to reversible fluorescence switching of enhanced green fluorescent proteins (EGFP) on indium tin oxide coated glass. The method allows efficient switching between bright (ON) and dim (OFF) states at the single molecule level. The electrochemical fluorescence switching is fast, reversible, and can be incorporated into advanced fluorescence microscopy.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Daniel E. Hagness, Ying Yang, Richard D. Tilley, J. Justin Gooding
Summary: Affinity biosensors play a crucial role in various areas of human health, such as clinical diagnosis and pharmaceuticals, by utilizing specific binding between target analytes and biological ligands. Electrokinetic phenomena have been investigated as a viable option to improve the performance of affinity biosensors for higher sensitivity and lower detection limit.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Seyedyousef Arman, Vinicius R. R. Goncales, Ying Yang, Richard D. D. Tilley, Katharina Gaus, J. Justin Gooding
Summary: This study explores a dual optical and electrical biosensor based on cells, which provides insights into cellular events. The fabrication steps and electrical characterization of microelectrodes are described. Initial experiments show that the ability of indium tin oxide (ITO) to detect biological cells at the electrode-cell layer interface mainly depends on the size of the sensing area. The impact of conductivity on the real-time impedance signal during cell adhesion on different substrates is also explored.
Review
Chemistry, Analytical
Seyedyousef Arman, Richard D. Tilley, J. Justin Gooding
Summary: This article reviews the development of cellular impedance biosensors, electrochemical impedance spectroscopy, and the general principles and terms associated with the cell-electrode interface. This family of techniques provides real-time quantitative and sensitive information on cell responses to stimuli with high temporal resolution, and their applications in cell biology are illustrated with various examples. The current state of the field, its limitations, possible solutions, and the potential benefits of developing biosensors are discussed.