Article
Plant Sciences
Eng Shi Ong, Christina Liu Ying Oh, Joseph Choon Wee Tan, Su Yi Foo, Chen Huei Leo
Summary: The study used PHWE to extract bioactive phytochemicals from different parts of okra, revealing that okra seeds have the highest antioxidant capacity and antidiabetic effects, likely due to their higher levels of polyphenolic compounds. Okra seeds also showed a reduction in vascular inflammation.
Article
Biochemistry & Molecular Biology
Xianshuang Cao, Yaoyao Zhang, Hang Xun, Jin Wang, Feng Tang
Summary: A pressurized hot water extraction (PHWE) method was developed to recover phenolic compounds from Bambusa chungii culms. The optimal extraction conditions were determined, and the extracts showed higher antioxidant activity compared to conventional solvent extractions.
Review
Chemistry, Analytical
Merichel Plaza, Maria Luisa Marina
Summary: Pressurized hot water extraction (PHWE) is a sustainable technique for extracting bioactive compounds without using organic solvents. This review provides an overview of PHWE fundamentals and its applications in recovering bioactive compounds from 2019 onwards. It also discusses the latest trends in PHWE, including its combination with other advanced extraction techniques and the use of natural deep eutectic solvents to enhance extraction yields.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Chemical
Debora Tamires Vitor Pererira, Francisco Manuel Barrales, Ericsem Pereira, Juliane Vigano, Amadeu Hoshi Iglesias, Felix Guillermo Reyes Reyes, Julian Martinez
Summary: Ultrasound-Assisted Pressurized Liquid Extraction (UAPLE) and Nanofiltration (NF) in 70% ethanol were found effective in recovering and concentrating bioactive compounds, mainly isoorientin, from passion fruit rinds. However, membrane surface fouling was observed during filtration process, leading to reduced permeate mass fluxes. Overall, integrated UAPLE and NF process shows promise for phenolic compounds recovery from passion fruit rinds.
JOURNAL OF FOOD ENGINEERING
(2022)
Article
Chemistry, Physical
Camila Mota Martins, Jhonyson Arruda Carvalho Guedes, Edy Sousa de Brito, Sandra Regina Salvador Ferreira
Summary: Supercritical fluid extraction and pressurized liquid extraction were combined to recover nonpolar and polar fractions from tamarind seeds. The combined extraction method resulted in increased total phenolic content and antioxidant capacity of the final extract.
JOURNAL OF SUPERCRITICAL FLUIDS
(2022)
Article
Biotechnology & Applied Microbiology
Mari Carmen Ruiz-Dominguez, Jose Antonio Mendiola, Jose David Sanchez-Martinez, Monica Bueno, Pedro Cerezal-Mezquita, Elena Ibanez
Summary: This study reveals the potential of the brown seaweed Durvillaea antarctica, commonly consumed in Chile, as an important food with antioxidant and neuroprotective properties. The bioactivity of the seaweed extracts was evaluated using conventional and pressurized liquid extraction methods. The results showed that pressurized liquid extraction improved the extraction yield and antioxidant activities compared to conventional extraction. Furthermore, the study identified the optimal conditions for obtaining extracts with high yields and neuroprotective effects.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Food Science & Technology
Ravshanbek Sultanbekovich Alibekov, Siti Mazlina Mustapa Kamal, Farah Saleena Taip, Alifdalino Sulaiman, Abdugani Mutalovich Azimov, Klara Abdyrazahovna Urazbayeva
Summary: This study focused on utilizing subcritical water to extract phenolic compounds from jackfruit seeds and correlate them with antioxidant activity. The highest total phenolic compounds (TPC) and antioxidant activity (AA) were obtained at 210°C, 30 min, and 15% solid loading under subcritical water extraction, with TPC of 1.84 mg GAE/100 g and AA of 86%. The subcritical water extraction obtained a higher amount of TPC compared to Soxhlet extraction.
Article
Biology
Cid-Perez Teresa Soledad, Hernandez-Carranza Paola, Ochoa-Velasco Carlos Enrique, Ruiz-Lopez Irving Israel, Nevarez-Moorillon GuadalupeVirginia, Avila-Sosa Raul
Summary: This study evaluated the volatile chemical profiling and biological activities of avocado seed extracts. Acetone was found to be the best solvent for extracting volatile compounds with high reducing power. The avocado seed extracts showed potential applications as antioxidants and antimicrobial additives.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Food Science & Technology
Nils Leander Huaman-Castilla, Cecilia Copa-Chipana, Luis Omar Mamani-Apaza, Olivia Magaly Luque-Vilca, Clara Nely Campos-Quiroz, Franz Zirena-Vilca, Maria Salome Mariotti-Celis
Summary: This study evaluates the use of isopropanol as a low-cost solvent for recovering polyphenols from discarded blueberries. The results show that using high concentrations of isopropanol and high temperatures leads to the highest content of polyphenols and antioxidant capacity. Additionally, using 30% water-isopropanol mixture improves the yield and removal of reducing sugars.
Article
Food Science & Technology
Beatriz de la Fuente, Noelia Pallares, Francisco J. Barba, Houda Berrada
Summary: This study evaluated the presence of heavy metals and mycotoxins in sea bass side streams and optimized the extraction conditions using Pressurized Liquid Extraction technique. The optimal conditions significantly increased protein content and antioxidant capacity of the extracts compared to conventional extraction methods. Additionally, different extracts showed variations in protein composition and antioxidant capacity.
Article
Food Science & Technology
M. Guzman-Lorite, M. L. Marina, M. C. Garcia
Summary: This study successfully achieved the recovery and characterization of proteins from pomegranate seeds using natural deep eutectic solvents (NADES) and pressurized liquid extraction (PLE). The combination of the two methodologies doubled the total recovery of proteins and revealed different protein selectivity.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Sakeena Si Yu Tan, Meyammai Shanmugham, Yu Ling Chin, Jia An, Chee Kai Chua, Eng Shi Ong, Chen Huei Leo
Summary: This study extracts phytochemical compounds from mangosteen pericarp using pressurized hot water extraction (PHWE) and investigates its biological effects on endothelial cells using RNA sequencing. Results show that extraction at a temperature of 120 degrees Celsius yields the highest concentrations of key phytochemical compounds and improves antioxidant capacity. Treatment of endothelial cells with the extract activates the AMPK signaling pathway and affects biological processes related to steroid biosynthesis and metabolism. Overall, this study provides important insights into the potential beneficial effects of phytochemical compounds from mangosteen pericarp on cardiometabolic health.
Article
Biochemistry & Molecular Biology
Alexandra Del-Castillo-Llamosas, Fernando Rodriguez-Rebelo, Beatriz Rodriguez-Martinez, Adrian Mallo-Fraga, Pablo G. Del-Rio, Beatriz Gullon
Summary: Avocado seeds, as the main waste produced in avocado processing, not only cause environmental problems but also economic losses. Deep eutectic solvents (DES) have been found to be a greener alternative for extracting bioactive compounds and carbohydrates from avocado seeds. The study showed that DES is an efficient and eco-friendly solvent to recover phenolics and carbohydrates from avocado seeds, which can be used as valuable bioresources.
Article
Environmental Sciences
Priti Ganorkar, G. C. Jadeja, Meghal A. Desai
Summary: Shikimic acid was extracted from water hyacinth using sonication to improve the recovery and antioxidant activity. The study found that parameters such as sonication time, solvent composition, and sonication amplitude had a significant effect on the extraction of shikimic acid.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Agriculture, Multidisciplinary
Gulay Ozkan
Summary: This study used natural deep eutectic solvents (NADES) coupled with ultrasound-assisted extraction to recover bioactive compounds from artichoke outer petals. The results showed that the use of choline chloride and lactic acid mixtures was superior in terms of obtaining extracts with high levels of phenolic compounds and antioxidant potential. The study highlights the potential of NADES combined with ultrasonication for the extraction of bioactive compounds from various plants and their incorporation into food and pharmaceutical formulations.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Biochemical Research Methods
Ming Yuan Heng, Beng Joo Reginald Thio, Eng Shi Ong
JOURNAL OF CHROMATOGRAPHIC SCIENCE
(2016)
Article
Chemistry, Applied
Ming Yuan Heng, Shigeru Katayama, Takakazu Mitani, Eng Shi Ong, Soichiro Nakamura
Article
Neurosciences
Suku-Maran Shalini, Christabel Fung-Yih Ho, Yee-Kong Ng, Jie-Xin Tong, Eng-Shi Ong, Deron R. Herr, Gavin S. Dawe, Wei-Yi Ong
MOLECULAR NEUROBIOLOGY
(2018)
Article
Chemistry, Applied
Feng Liu, Eng Shi Ong, Sam Fong Yau Li
Review
Chemistry, Analytical
Ming Yuan Heng, Swee Ngin Tan, Jean Wan Hong Yong, Eng Shi Ong
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2013)
Article
Plant Sciences
Eng Shi Ong, Christina Liu Ying Oh, Joseph Choon Wee Tan, Su Yi Foo, Chen Huei Leo
Summary: The study used PHWE to extract bioactive phytochemicals from different parts of okra, revealing that okra seeds have the highest antioxidant capacity and antidiabetic effects, likely due to their higher levels of polyphenolic compounds. Okra seeds also showed a reduction in vascular inflammation.
Article
Biochemistry & Molecular Biology
Chen Huei Leo, Su Yi Foo, Joseph Choon Wee Tan, U-Xuan Tan, Chee Kai Chua, Eng Shi Ong
Summary: This study aims to extract bioactive compounds from orange peel waste (OPW) using a green extraction method called pressurized hot water extraction (PHWE). The results showed that extraction temperatures of 60 degrees C and 80 degrees C yielded distinct bioactive compounds with better antioxidant capacity. Specifically, treatment with 60 degrees C OPW extracts inhibited vascular inflammation and endothelial dysfunction in vitro, suggesting its vasoprotective effects mediated by anti-inflammatory effects.
Article
Medicine, Research & Experimental
Chen Huei Leo, Jamie Li Min Ou, Eng Shi Ong, Cheng Xue Qin, Rebecca H. Ritchie, Laura J. Parry, Hooi Hooi Ng
Summary: This study evaluates the protective effects of relaxin on diabetic kidney complications. The results show that relaxin treatment can reduce key markers of kidney fibrosis, oxidative stress, and inflammation in a diabetic mouse model, and increase the levels of bile acid metabolites. Therefore, relaxin may serve as an adjunctive treatment for diabetic kidney complications.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Eng Shi Ong
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Analytical
Eng Shi Ong
Summary: Bioactive compounds in functional foods, medicinal plants and others are considered valuable for their wide range of bioactivity. Urine is an effective biofluid for evaluating and monitoring changes in homeostasis and metabolism. The review discusses the formation of urine, sampling and analysis of urinary metabolites, and recent developments in metabolite profiling. Advances in LC/MS/MS, chemical fragmentation libraries, computational resources, and data analysis approaches have made urine profiling an attractive option. Profiling urinary metabolites provides insights into diet-related biomarkers and their biological effects.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2023)
Review
Food Science & Technology
Chen Huei Leo, Eng Shi Ong
Summary: Functional foods and their by-products have various bioactive components that offer health benefits and can enhance public health and well-being. Environmentally friendly, sustainable, and cost-effective methods are needed to extract flavonoids, phenolic compounds, and other substances from functional food and agri-food wastes to achieve a circular economy. This research covers extraction methods that eliminate the use of organic solvents and are suitable for laboratory and commercial production. Analytical methods are used to determine marker or bioactive compounds and chemical fingerprints to control the composition variation of functional food products. Combining chemical standardization with antioxidant assays provides information on extract quality. Cellular models and other experimental endpoints, such as metabolomics, are used to validate the antioxidant properties of the extracts.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Sakeena Si Yu Tan, Meyammai Shanmugham, Yu Ling Chin, Jia An, Chee Kai Chua, Eng Shi Ong, Chen Huei Leo
Summary: This study extracts phytochemical compounds from mangosteen pericarp using pressurized hot water extraction (PHWE) and investigates its biological effects on endothelial cells using RNA sequencing. Results show that extraction at a temperature of 120 degrees Celsius yields the highest concentrations of key phytochemical compounds and improves antioxidant capacity. Treatment of endothelial cells with the extract activates the AMPK signaling pathway and affects biological processes related to steroid biosynthesis and metabolism. Overall, this study provides important insights into the potential beneficial effects of phytochemical compounds from mangosteen pericarp on cardiometabolic health.
Article
Engineering, Biomedical
Jian Da Tan, Cheng Pau Lee, Su Yi Foo, Joseph Choon Wee Tan, Sakeena Si Yu Tan, Eng Shi Ong, Chen Huei Leo, Michinao Hashimoto
Summary: This study explores the use of 3D printing technology to transform orange peel waste into edible, healthy snacks, aiming to address the detrimental environmental and economic impacts of discarding such waste. Through direct ink writing, edible constructs containing orange peel waste were successfully created. Biochemical analysis and cell viability tests revealed that the nutritional and antioxidant content of the orange peel waste was not affected during the manufacturing process. This study highlights the potential of revalorizing food waste and achieving more sustainable and circular food manufacturing and consumption through 3D printing.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Biochemistry & Molecular Biology
Eng Shi Ong, Charlene Jia Ning Pek, Joseph Choon Wee Tan, Chen Huei Leo