Review
Biochemistry & Molecular Biology
Yuyang Li, Min Liu, Songbo Xie
Summary: Phage display is a powerful high-throughput screening technology widely used in bioengineering and biomedicine for affinity determination towards a target by presenting functional peptides, proteins, or antibody fragments on phage capsid surfaces. With the advancement of next-generation sequencing and microfluidics, phage display has become an even more powerful and popular tool for drug discovery and development.
CURRENT MEDICINAL CHEMISTRY
(2021)
Review
Virology
Kazuya Nagano, Yasuo Tsutsumi
Summary: Antibody drugs with high affinity and specificity are effective for intractable diseases, such as cancers and autoimmune diseases. The recent breakthrough in antibody drugs is largely due to the contribution of phage display technology.
Review
Microbiology
Weronika Jaroszewicz, Joanna Morcinek-Orlowska, Karolina Pierzynowska, Lidia Gaffke, Grzegorz Wegrzyn
Summary: Phage display technology is a widely applicable method in biotechnology, recognized by the Nobel Prize. This article provides an overview of the technology, including comparisons of different display systems, their advantages and disadvantages, and examples of applications in various fields. It also discusses other peptide display technologies and highlights the ongoing development and improvements in these methods, offering new solutions in the future.
FEMS MICROBIOLOGY REVIEWS
(2022)
Review
Immunology
Marco Palma
Summary: Traditional vaccines using inactivated or weakened forms of pathogens may have side effects and inadequate immune responses. Phage display has emerged as a valuable tool for identifying specific epitopes that could be used in vaccines. It offers exceptional efficiency and speed, making it promising in critical scenarios such as pandemics.
Article
Multidisciplinary Sciences
Baerbel Schroefelbauer, Patrick K. Kimes, Paige Hauke, Charlotte E. Reid, Kevin Shao, Sarah J. Hill, Rafael Irizarry, William C. Hahn
Summary: In this study, a high-throughput platform was developed to simultaneously discover antibodies and cancer-specific targets based on phenotypic binding profiles. Utilizing genomics, flow cytometry, and mass spectrometry, a wide diversity of cancer targets including receptor tyrosine kinases, adhesion and migration proteins, proteases, and proteins regulating angiogenesis were identified in ovarian cancer. BCAM was identified as a promising candidate for targeted therapy in high-grade serous ovarian cancers. Overall, this approach provides a rapid and flexible framework to identify cancer targets and antibodies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Microbiology
Marek Straka, Martina Dubinova, Adriana Liptakova
Summary: The treatment of bacterial infections has become more complicated due to the rise of antibiotic-resistant strains. Bacteriophage therapy, which uses natural predators of bacteria, shows great potential as an alternative. Although the discovery of antibiotics initially hampered the development of phage therapy, Western countries are now reintroducing it as a tool to combat drug-resistant bacteria.
Article
Biochemistry & Molecular Biology
Tulika Tulika, Rasmus W. Pedersen, Charlotte Rimbault, Shirin Ahmadi, Esperanza Rivera-de-Torre, Monica L. Fernandez-Quintero, Johannes R. Loeffler, Markus-Frederik Bohn, Anne Ljungars, Line Ledsgaard, Bjorn G. Voldborg, Fulgencio Ruso-Julve, Jan Terje Andersen, Andreas H. Laustsen
Summary: Recycling IgG antibodies can bind to antigens at physiological pH and release them at lower pH, achieving comparable therapeutic effect at lower doses. The pH-dependent antigen-antibody binding may not always be driven by histidine residues. The study presents a methodology using a natural antibody phage display library to discover recycling antibodies without the need for histidine doping.
Article
Biochemistry & Molecular Biology
Tulika Tulika, Rasmus W. Pedersen, Charlotte Rimbault, Shirin Ahmadi, Esperanza Rivera-de-Torre, Monica L. Fernandez-Quintero, Johannes R. Loeffler, Markus-Frederik Bohn, Anne Ljungars, Line Ledsgaard, Bjorn G. Voldborg, Fulgencio Ruso-Julve, Jan Terje Andersen, Andreas H. Laustsen
Summary: In this study, a new method was proposed to discover antibodies that bind alpha-cobratoxin from the venom of Naja kaouthia in a pH-dependent manner using a naive antibody phage display library. The discovered antibody showed pH-dependent binding without histidine residues and exhibited higher off-rate at pH 5.5 compared to pH 7.4. Molecular dynamics simulations suggested that different protonation states of titratable residues might contribute to the pH-dependent binding properties of the antibody. This method can be used to discover recycling antibodies against different targets without the need for histidine doping.
Article
Infectious Diseases
Mikael Skurnik
Summary: Due to the increasing antibiotic resistance, it is necessary to address the issue promptly. Phage therapy, as a precision therapy for bacterial infections, has gained attention, but there are still unresolved issues such as clear instructions, practical experience, and seamless coordination.
Review
Medicine, Research & Experimental
Carola Venturini, Aleksandra Petrovic Fabijan, Alicia Fajardo Lubian, Stefanie Barbirz, Jonathan Iredell
Summary: Bacteriophages are selective viral predators of bacteria and can be used to treat bacterial infections. However, there are challenges to implementing phage therapy into medical practice, including a lack of clinical trial data and a need for a better understanding of bacterium-phage interactions.
EMBO MOLECULAR MEDICINE
(2022)
Article
Immunology
Chao-Yang Huang, Ying-Yung Lok, Chia-Hui Lin, Szu-Liang Lai, Yen-Yu Wu, Chih-Yung Hu, Chu-Bin Liao, Chen-Hsuan Ho, Yu-Ping Chou, Yi-Hsuan Hsu, Yu-Hsun Lo, Edward Chern
Summary: In this study, a large and highly diverse synthetic human scFv antibody library was constructed, and novel TIM-3-neutralizing antibodies with immunomodulatory functions were identified. These findings demonstrate the potential of this library for biomedical research and the therapeutic potential of the three fully human TIM-3-neutralizing antibodies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Engineering, Environmental
Zhuodong Yu, Cory Schwarz, Liang Zhu, Linlin Chen, Yun Shen, Pingfeng Yu
Summary: The study demonstrates that the lytic coliphage PHHO1 can hitchhike on carrier bacteria Bacillus cereus to infect host bacteria more effectively in biofilms. This mutualistic relationship enhances bacterial colonization, influences biofilm composition, and has the potential to mediate biofilm functions and moderate associated risks.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Review
Chemistry, Medicinal
Carolina Moraes de Souza, Tayfun Tanir, Marvin Orellana, Aster Escalante, Michael Sandor Koeris
Summary: This section discusses the process of manufacturing drug products from bacteriophage drug substances, emphasizing the various tradeoffs that need to be made in pharmaceutical development. It also introduces some historical and current analytical methods to consider when formulating bacteriophage products.
Review
Food Science & Technology
Guillermo Ortiz Charneco, Paul P. de Waal, Irma M. H. van Rijswijck, Noel N. M. E. van Peij, Douwe van Sinderen, Jennifer Mahony
Summary: Bacteriophages pose a persistent threat to food fermentations, particularly large-scale commercial dairy fermentations. The interaction between phages and lactic acid bacteria used as starter cultures in dairy fermentations has been extensively studied, leading to advances in understanding coevolution and the development of robust starter cultures. This review highlights recent progress in phage-host interactions and phage resistance mechanisms in different bacterial species, and discusses their impact on the dairy fermentation industry and future plant-based food fermentations.
ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sa Dong, Lingjun Guan, Kangli He, Wenchao Yang, Wei Deng, Shuzhong Yuan, Jianguo Feng
Summary: The study utilized the anti-idiotypic antibody technique to screen for clones from a human domain antibody library that can simulate Cry1A toxins, with clone D6 showing the most significant competitive binding ability with anti-Cry1A polyclonal antibodies. This provides a potential avenue for the development of Cry toxin simulants.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Medicinal
Maryam Parandoosh, Reyhaneh Yousefi, Hoda Khorsandi, Omid Nikpayam, Atoosa Saidpour, Hossein Babaei
PHYTOTHERAPY RESEARCH
(2020)
Review
Pharmacology & Pharmacy
Maryam Dashtiahangar, Leila Rahbarnia, Safar Farajnia, Arash Salmaninejad, Arezoo Gowhari Shabgah, Samaneh Ghasemali
Summary: Recombinant immunotoxins (RITs) have revolutionized cancer treatment by targeting and eliminating cancerous cells through the fusion of antibodies to toxin proteins. However, high immunogenicity remains a major obstacle in clinical use, and various strategies have been proposed to address this limitation.
CURRENT PHARMACEUTICAL DESIGN
(2021)
Review
Pharmacology & Pharmacy
Jafar Sharifi, Mohammad Reza Khirehgesh, Fatemeh Safari, Bahman Akbari
Summary: This review discusses the use of nanobodies in targeted therapy, focusing on recent advancements and future prospects in targeting EGFR.
JOURNAL OF DRUG TARGETING
(2021)
Review
Pharmacology & Pharmacy
Mohammad Reza Khirehgesh, Jafar Sharifi, Fatemeh Safari, Bahman Akbari
Summary: Immunotoxins (ITs) are protein-based drugs composed of targeting and cytotoxic moieties, successful in treating hematologic malignancies. The use of nanobodies (Nbs) as a targeting moiety may help overcome the poor penetration of ITs into solid tumors.
JOURNAL OF DRUG TARGETING
(2021)
Article
Biochemistry & Molecular Biology
Jafar Sharifi, Mohammad Reza Khirehgesh, Bahman Akbari, Bijan Soleymani, Kamran Mansouri
Summary: Targeted therapy is an effective approach in cancer treatment, and a novel humanized anti-EGFR biparatopic nanobody, hu7CG2, showed potential in inhibiting the growth of EGFR-overexpressing tumor cells by grafting specific regions from camelid anti-EGFR nanobodies.
MOLECULAR BIOTECHNOLOGY
(2021)
Review
Infectious Diseases
Fariba Lotfi, Mostafa Akbarzadeh-Khiavi, Ziba Lotfi, Leila Rahbarnia, Azam Safary, Habib Zarredar, Amir Baghbanzadeh, Behrooz Naghili, Behzad Baradaran
Summary: Micronutrients such as vitamins and minerals play a crucial role in improving immune system function and accelerating treatment processes. Dietary supplements can help attenuate vascular and inflammatory manifestations related to infectious diseases.
Review
Infectious Diseases
Mostafa Akbarzadeh-Khiavi, Mitra Torabi, Leila Rahbarnia, Azam Safary
Summary: The combination therapy of Janus kinase (JAK) inhibitor baricitinib has shown positive therapeutic outcomes in hospitalized COVID-19 patients, by modulating the inflammatory response and controlling cytokine storm induction. This strategy may be a potential treatment option for severe cases of COVID-19 with the standard of care.
Article
Pharmacology & Pharmacy
Mohammad Reza Khirehgesh, Jafar Sharifi, Bahman Akbari, Kamran Mansouri, Fatemeh Safari, Bijan Soleymani, Kheirollah Yari
Summary: Targeted therapy aims to improve the efficacy of cancer treatment by selecting appropriate cell surface markers. A novel humanized anti-EGFR immunotoxin was developed in this study, showing potency against EGFR-positive cancers and presenting a promising tool for targeting EGFR-positive tumors.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Correction
Infectious Diseases
Fariba Lotfi, Mostafa Akbarzadeh-Khiavi, Ziba Lotfi, Leila Rahbarnia, Azam Safary, Habib Zarredar, Amir Baghbanzadeh, Behrooz Naghili, Behzad Baradaran
Article
Medicine, Research & Experimental
Abbas Navabi, Bahman Akbari, Mohammad Abdalsamadi, SeyedSaeid Naseri
Summary: This review summarizes the roles of miRNAs in the development, progression, and drug resistance of CML, as well as their potential role in the diagnosis, prognosis, and treatment response of CML.
Review
Biotechnology & Applied Microbiology
Mostafa Akbarzadeh-Khiavi, Mitra Torabi, Amir-Hossein Olfati, Leila Rahbarnia, Azam Safary
Summary: This article provides a comprehensive insight into the strategies used for the conjugation and modification of Melittin (MLT), as well as its cellular delivery and clinical perspectives. MLT, as a natural drug, has remarkable biological properties, but its clinical translation is hindered by low stability, high toxicity, and weak tissue penetration. Various bio-nano scale modifications show promise in improving its functionality and therapeutic efficacy.
EXPERT OPINION ON BIOLOGICAL THERAPY
(2022)
Article
Chemistry, Physical
Zahra Maani, Safar Farajnia, Leila Rahbarnia, Elaheh Zadeh Hosseingholi, Nazli Khajehnasiri, Parisa Mansouri
Summary: The interaction between CD147/Cyp A is a critical pathway for certain cancer types and the entry of COVID-19 virus into host cells. A hybrid peptide derived from Melittin and TAT was designed to inhibit the CD147/Cyp A interaction. The hybrid peptide showed potential as a therapeutic candidate for tumor types and COVID-19 infection.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Biotechnology & Applied Microbiology
Parisa Mansouri Rad, Leila Rahbarnia, Azam Safary, Azizeh ShadiDizaji, Zahra Maani
Summary: Two new analogs of melittin (MLT) were designed and synthesized to increase stability, reduce toxicity, and retain antimicrobial properties against bacterial pathogens.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Biology
Safar Farajnia, Leila Rahbarnia, Nazli Khajehnasiri, Habib Zarredar
Summary: The aim of this study was to design a novel antimicrobial peptide with high stability and low toxicity. Through fusion between mutant analogs of melittin and CXCL14-C17, and a series of experiments and simulation analysis, the TM1C2 peptide was designed, which showed higher antimicrobial and stability, and more potential for penetration into the lipid membrane.
Article
Biology
Fatemeh Safari, Bahman Akbari
Summary: This study assessed the effect of caspase-7 deficiency on CHO cell growth, viability, and protein expression. The findings showed that the absence of caspase-7 decreased cell growth and viability, but resulted in enhanced recombinant protein expression due to cell cycle arrest. Additionally, caspase-3 enzymatic activity increased in the absence of caspase-7 in apoptotic situations.
BIOLOGICAL RESEARCH
(2022)
