Article
Chemistry, Analytical
Francesca Costantini, Nicola Lovecchio, Manasa Nandimandalam, Ariana Manglli, Francesco Faggioli, Mara Biasin, Cesare Manetti, Pio Federico Roversi, Augusto Nascetti, Giampiero de Cesare, Domenico Caputo
Summary: In this study, a Lab-on-Chip (LoC)-PCR device called System-on-Glass (SoG) was developed, which integrated thermalization, temperature control, and detection elements on a single glass substrate. Real-time reverse transcriptase PCR of RNA from plant and human viruses was successfully conducted using this device. The LoC-PCR achieved the same detection limit and analysis time as the standard equipment, but with the advantage of portability and faster analysis.
Article
Chemistry, Multidisciplinary
Matteo Agostini, Francesco Lunardelli, Mariacristina Gagliardi, Alessio Miranda, Leonardo Lamanna, Antonella Giuliana Luminare, Francesca Gambineri, Michele Lai, Mauro Pistello, Marco Cecchini
Summary: Measles is highly infectious, and despite the availability of a vaccine, it remains common in developing countries and sporadic outbreaks occur in developed countries. This study presents a point-of-care biosensing device capable of detecting measles virus in human saliva, with promising results for measles diagnosis and guidance for designing new biosensing systems.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Chemistry, Multidisciplinary
Samaneh Zare Harofte, Madjid Soltani, Saeed Siavashy, Kaamran Raahemifar
Summary: Nowadays, artificial intelligence creates numerous opportunities in the life sciences. Integrating AI with microfluidics can significantly impact various fields of biotechnology.
Article
Biochemical Research Methods
Ning Zhang, Chao Yue, Xiaobo Zhan, Zhi Cheng, Chao Li, Yaohua Du, Feng Tian
Summary: The quantitative analysis of respiratory viruses is important for diagnosis, medicine and prognosis. In this study, a lab-on-a-chip platform combined with an automated control system was used to achieve quantitative analysis of COVID-19 pseudovirus from large-volume nasal swab samples. The method can be extended to other respiratory viruses and sample types.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Sangam Srikanth, U. S. Jayapiriya, Satish Kumar Dubey, Arshad Javed, Sanket Goel
Summary: A simple, cost-effective and miniaturized lab-on-a-chip platform has been developed for simultaneous cultivation and detection of bacteria, with high accuracy in quantifying bacterial concentration and validating viability, as well as real-time and on-field applications.
Article
Materials Science, Multidisciplinary
Zhenzhen Chen, Liang Shen, Xiong Zhao, Hongqiang Chen, Yaxuan Xiao, Yonghai Zhang, Xiaoping Yang, Jinhua Zhang, Jinjia Wei, Nanjing Hao
Summary: Microfluidics, especially acoustofluidics, offers new opportunities in chemical and biomedical engineering by manipulating fluids at the micrometer or submicrometer scale. This review discusses the utility of acoustofluidic technology, summarizes different types of acoustic micromixers, highlights their applications in various fields, and identifies the practical challenges that need to be addressed. Overall, this review provides valuable insights for both academic research and industrial applications.
APPLIED MATERIALS TODAY
(2022)
Review
Pharmacology & Pharmacy
Renata Maia, Violeta Carvalho, Rui Lima, Graca Minas, Raquel O. Rodrigues
Summary: This systematic review summarizes the recent progress in the use of microneedles (MNs) in biomedical applications, including drug delivery, biomarker detection, and their potential integration with microfluidic devices. Although MNs have been extensively utilized in lab-on-a-chip platforms, recent studies have also explored their applicability in organ-on-a-chip models. Overall, the presence of MNs in advanced microfluidic devices offers simplified drug delivery, microinjection, and biomarker detection through integrated biosensors, enabling real-time monitoring of various biomarkers in lab- and organ-on-a-chip platforms.
Article
Biochemistry & Molecular Biology
Emanuele Piccinno, Anna Grazia Monteduro, Francesco Dituri, Silvia Rizzato, Gianluigi Giannelli, Giuseppe Maruccio
Summary: The study validated a miniaturized on-chip platform for real-time, non-destructive cell proliferation assays and drug screening, comparing its effectiveness with the traditional 96-well plate assay. Results showed the new platform's efficacy comparable to the standard method, with advantages in reagent consumption, operator time, and costs, potentially accelerating drug development processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Canan Ozyurt, Inci Uludag, Bahar Ince, Mustafa Kemal Sezginturk
Summary: Lab-on-a-chip (LOC) or micro total analysis system is a microfluidic technology that integrates and automates analytical laboratory procedures into a single instrument or chip. This article reviews the recent developments in the application of LOC biosensors for cancer detection. Microfluidics has revolutionized healthcare diagnosis by enabling the reconstruction of functional organ units on chips and studying human diseases like cancer. In the past five years, LOCs have been widely used in various biomedical applications such as bioassays, cell analysis, drug discovery, and personalized medicine. This review provides an overview of microfluidic-based cancer research, including its pros, cons, and future prospects.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Stefan Gassmann, Till Schleifer, Helmut Schuette
Summary: This paper presents a Lab-on-a-chip platform for colorimetric measurements in water, providing an effective method for monitoring nutrients in open water. The platform can be customized for different analytes by changing reagents and detection wavelength.
Article
Chemistry, Analytical
Alexandro Catini, Rosamaria Capuano, Giuseppe Tancredi, Giulio Dionisi, Davide Di Giuseppe, Joanna Filippi, Eugenio Martinelli, Corrado Di Natale
Summary: This paper presents an automatic chemical assay platform for continuous monitoring of harmful compounds in aquaculture. The platform uses a lab-on-a-chip approach and low-cost optoelectronic components to analyze water samples, providing a reliable and cost-effective solution.
Article
Chemistry, Physical
Cristina Chircov, Alexandra Catalina Birca, Alexandru Mihai Grumezescu, Bogdan Stefan Vasile, Ovidiu Oprea, Adrian Ionut Nicoara, Chih-Hui Yang, Keng-Shiang Huang, Ecaterina Andronescu
Summary: The study introduced a new method for the synthesis of magnetite nanoparticles using a microfluidic lab-on-chip device to achieve controlled properties. It investigated the influence of iron precursor solution concentration and flow on the final properties of the nanomaterials, ultimately achieving uniformity and stability in the synthesized nanoparticles.
Article
Chemistry, Multidisciplinary
Nicolas Rolley, Marie Bonnin, Guillaume Lefebvre, Sylvain Verron, Sylwester Bargiel, Laurent Robert, Jeremie Riou, Carl Simonsson, Thomas Bizien, Jean-Christophe Gimel, Jean-Pierre Benoit, Guillaume Brotons, Brice Calvignac
Summary: This study focuses on continuous production of drug delivery systems using microfluidics for high reproducibility and controlled particle size distributions. GALECHIP concept is proposed for formulating nanomedicines like LNCs, with chips manufactured using 3D printing and DRIE technologies. The transparent Si/Glass chip is used for small angle X-ray scattering analysis of LNC formulation. Discussions revolve around costs and advantages of the technologies in a GALECHIP point of view.
Article
Medical Laboratory Technology
Sneha Venkatesalu, Shanmugapriya Dilliyappan, Avanthika Satish Kumar, Thirunavukkarasu Palaniyandi, Gomathy Baskar, Maddaly Ravi, Asha Sivaji
Summary: Microfluidics is a science and technology that deals with less sample-to-more precision in vitro analysis. It has wide applications in cancer theranostics, enabling precise diagnosis and personalized treatment.
CLINICA CHIMICA ACTA
(2024)
Article
Multidisciplinary Sciences
Michael Sieber, Arne Traulsen, Hinrich Schulenburg, Angela E. Douglas
Summary: The prevalence of specific microbes in a host population can increase even when they do not provide specific benefits to the host, especially when there is overlap in their lifecycles and they share dispersal routes. This suggests that host enrichment alone is not a reliable indicator of beneficial host-microbe interactions, but increased time spent associated with a host can lead to new selection conditions, potentially favoring microbial adaptations towards a host-associated lifestyle and laying the foundation for the evolution of mutually beneficial coevolved symbioses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Medicine, General & Internal
Fernando L. Gordillo Altamirano, Xenia Kostoulias, Dinesh Subedi, Denis Korneev, Anton Y. Peleg, Jeremy J. Barr
Summary: This study demonstrates that the combination of phage and ceftazidime has a significant bactericidal effect against Acinetobacter baumannii AB900 in vivo. The combination treatment group showed a lower bacterial burden compared to the other treatment groups, suggesting that the combination therapy may be an effective strategy for treating multidrug-resistant Acinetobacter baumannii infections.
Article
Microbiology
Yuxin Wang, Dinesh Subedi, Jin Li, Jiaoling Wu, Jianluan Ren, Feng Xue, Jianjun Dai, Jeremy J. Barr, Fang Tang
Summary: Phage therapy shows promising potential in combating enteric pathogens and promoting the recovery of infected mice compared to antibiotics.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemical Research Methods
Bryan Ang, Ankush Sookram, Citsabehsan Devendran, Vincent He, Kellie Tuck, Victor Cadarso, Adrian Neild
Summary: Surface acoustic wave (SAW) driven devices often use polymeric microfluidic channels with low acoustic impedance mismatch to the fluid in contact for precise control of the wave field. This work demonstrates the successful implementation of integrating a glass insert at the ceiling of the PDMS microfluidic channel in a SAW activated nanosieve, resulting in a significant increase in flow rate and maintenance of high capture efficiencies. The glass-inserted device allows for processing larger volume samples, overcoming a main limitation of these devices.
Article
Chemistry, Analytical
Bryan Ang, Ruhollah Habibi, Ciaren Kett, Wai Hoe Chin, Kellie L. Tuck, Adrian Neild, Victor J. Cadarso
Summary: Microfluidic concentration technology can detect low concentrations of bacteria in samples, making it suitable for time-sensitive situations such as clinical settings and food quality control. By using a packed bed of micro-particles activated by surface acoustic waves, bacteria can be efficiently captured and recovered for further analysis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemistry & Molecular Biology
Aysha L. Sezmis, Laura C. Woods, Anton Y. Peleg, Michael J. McDonald
Summary: Horizontal gene transfer (HGT) is crucial for microbial evolution, allowing genetic variants to cross species barriers even in the absence of strong selection. This study investigates HGT between Acinetobacter baylyi and multidrug-resistant Acinetobacter baumannii strains, confirming that HGT can drive the spread of antibiotic resistance genes (ARGs) without selection for the antibiotic. The study also quantifies the costs and benefits of transferred variants and reveals the role of genetic elements in the spread of ARGs.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Multidisciplinary Sciences
Xiyang Dong, Yongyi Peng, Muhua Wang, Laura Woods, Wenxue Wu, Yong Wang, Xi Xiao, Jiwei Li, Kuntong Jia, Chris Greening, Zongze Shao, Casey R. J. Hubert
Summary: Little is known about genetic heterogeneity within deep sea cold seep microbial populations. This study examines the intraspecies diversity patterns of 39 abundant species identified in sediment layers below the sea floor across six cold seep sites. The results reveal different evolutionary trajectories at the genomic level among these diverse populations and highlight the interplay between ecological processes and the evolution of key bacteria and archaea in deep sea cold seep extreme environments.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Vincent He, Victor J. Cadarso, Susanne Seibt, Ben J. Boyd, Adrian Neild
Summary: In this study, a microfluidic approach was used to prepare different aqueous and lipid droplets and induce phase transformations in the liquid medium. The results showed that when the lipid droplets and aqueous droplets coalesced, distinct structures with different compositions formed on the two sides of the merged droplet. Through diffusion limited interface, water molecules gradually hydrated the lipid portion, leading to a series of phase transformations. This study provides a new approach for studying the kinetics of phase transformations and identifying non-equilibrium phases in droplet-based lyotropic liquid systems.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Meeting Abstract
Infectious Diseases
Y. Zhang, J. Zhao, Y. Lin, M. Han, Y. Zhu, J. Lu, A. Neild, A. Demarco, J. Li
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Biochemical Research Methods
Junyang Gai, Citsabehsan Devendran, Adrian Neild, Reza Nosrati
Summary: By using an acoustic streaming device, the study investigated the rheotaxis behavior of sperm in microchannels and found that changes in flow velocity could affect the trajectory of sperm.