Journal
FOOD CHEMISTRY
Volume 384, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.132495
Keywords
Electrochemical aptasensor; Zeolitic imidazolate framework-8; Gold nanoparticles; Aflatoxin B1
Funding
- Young Teachers Funded Projects of Sichuan Agricultural University [03120310]
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An electrochemical aptasensor was fabricated for sensitive detection of AFB1 using a modified ZIF-8. The developed aptasensor exhibited a wider linear range and lower detection limit, as well as outstanding selectivity, reproducibility and stability. The method successfully detected AFB1 in corn oil and peanut oil samples, demonstrating its potential application value.
In this study, an electrochemical aptasensor for sensitive detection of aflatoxin B1 (AFB1) was fabricated by electrodepositing gold nanoparticles (AuNPs) on the glass carbon electrode (GCE) modified with zeolitic imidazolate framework-8 (ZIF-8). The high specific surface area of AuNPs/ZIF-8 nanocomposite increased the aptamers loading on the electrode surface. When compared to other previously reported sensors, the developed aptasensor exhibited a wider linear range of 10.0 to 1.0 x 10(5) pg/mL with a lower limit of detection (LOD) of 1.82 pg/mL under the optimized conditions. Furthermore, the obtained results revealed that the constructed aptasensor exhibited outstanding selectivity, reproducibility and stability. Moreover, the aptasensor was successfully employed to detect the AFB1 in corn oil and peanut oil samples, with recoveries ranging from 93.49% to 106.9%, demonstrating the potential application value of this methodology.
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