Review
Biochemistry & Molecular Biology
Nicole Pui-Yu Ho, Hitoshi Takizawa
Summary: This article summarizes the current understanding of how hematopoietic stem cell functions are maintained, damaged, or exhausted during acute, prolonged, and pathological inflammatory conditions. It also highlights the impact of inflammation-altered hematopoietic stem cell niche on escalating the insults on hematopoietic stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Meng Zhu, Qiwei Wang, Tianning Gu, Yingli Han, Xin Zeng, Jinxin Li, Jian Dong, He Huang, Pengxu Qian
Summary: Haematopoietic Stem cells (HSCs) possess the ability to renew and differentiate into various lineages, which is regulated by the microenvironment. The use of hydrogels in tissue engineering has allowed for the mimicking of the HSC microenvironment in vitro, influencing HSC behavior and ultimately improving HSC acquisition. This review presents the recent advancements in hydrogel-based microenvironment engineering of HSCs and discusses future challenges in basic research and clinical practice.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Multidisciplinary Sciences
Claire Fielding, Andres Garcia-Garcia, Claudia Korn, Stephen Gadomski, Zijian Fang, Juan L. Reguera, Jose A. Perez-Simon, Berthold Gottgens, Simon Mendez-Ferrer
Summary: Cholinergic signals in the bone marrow preserve haematopoietic stem cell quiescence and self-renewal under proliferative stress.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Biomedical
Yinbo Xiao, ChanelleA. S. McGuinness, W. Sebastian Doherty-Boyd, Manuel Salmeron-Sanchez, Hannah Donnelly, Matthew J. Dalby
Summary: Hematopoietic stem cells play a fundamental role in the generation of blood and immune cells. Although the signaling mechanisms in their bone marrow niche microenvironment have been increasingly understood, the ability to recreate these mechanisms in vitro for clinical purposes is still lacking. Recent studies have used engineering techniques to establish ex vivo bone marrow niche models, showing promising potential for research and clinical applications.
Article
Cell Biology
Bo Li, Jingya Li, Bingzhi Li, Takehito Ouchi, Longjiang Li, Yu Li, Zhihe Zhao
Summary: Craniofacial bones provide support and protection for the brain, with stem cells and their niches playing a key role in bone turnover. However, comprehensive studies of the cranial stem cell niches and age-related changes are lacking. In this study, single-cell RNA sequencing was used to analyze the transcriptomic profiles and cellular compositions of cranial stem cell niches during aging. The results revealed alterations in the bone marrow microenvironment influenced by inflammaging, identified senescent mesenchymal cell subclusters and age-related immune cell subclusters, and analyzed differentially expressed genes and cell-cell communications, providing insights into cranial bone biology and potential therapeutic targets for antiaging and regenerative medicine.
Article
Cell Biology
Carl A. Mitchell, Evgenia V. Verovskaya, Fernando J. Calero-Nieto, Oakley C. Olson, James W. Swann, Xiaonan Wang, Aurelie Herault, Paul V. Dellorusso, Si Yi Zhang, Arthur Flohr Svendsen, Eric M. Pietras, Sietske T. Bakker, Theodore T. Ho, Berthold Gottgens, Emmanuelle Passegue
Summary: Haematopoietic ageing is characterized by a loss of regenerative capacity and abnormal differentiation of haematopoietic stem cells (HSCs), resulting in impaired blood production. The study reveals an inflammatory environment in the old bone marrow niche, which contributes to niche deterioration, HSC dysfunction, and impaired haematopoietic regeneration. The production of interleukin-1 beta (IL-1 beta) by damaged endosteum drives the proinflammatory nature of the central marrow, causing damage to the old blood system. Blocking IL-1 signaling is a potential strategy to improve blood production during ageing.
NATURE CELL BIOLOGY
(2023)
Article
Cell Biology
Miguel Ganuza, Trent Hall, Jacquelyn Myers, Chris Nevitt, Raul Sanchez-Lanzas, Ashley Chabot, Juan Ding, Emilia Kooienga, Claire Caprio, David Finkelstein, Guolian Kang, Esther Obeng, Shannon McKinney-Freeman
Summary: Research shows that hematopoietic stem cells (HSCs) in the fetal liver do not significantly expand during development compared to adult bone marrow HSCs. Although there is substantial proliferation of HSCs in the fetal liver, many cells tend to differentiate rather than self-renew.
NATURE CELL BIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Cornelia Lee-Thedieck, Peter Schertl, Gerd Klein
Summary: This review provides a comprehensive overview of the extracellular matrix (ECM) in hematopoietic stem cell (HSC) niches and highlights its importance in regulating cellular function and niche structure. The role of different classes of ECM molecules and their interactions with cells are discussed, along with the significance of matrix remodeling and biophysics in HSC niche function. The review also examines the application of current knowledge of ECM in artificial HSC niches for HSC expansion, targeted differentiation, and drug testing.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Engineering, Biomedical
Michael R. Nelson, Delta Ghoshal, Joscelyn C. Mejias, David Frey Rubio, Emily Keith, Krishnendu Roy
Summary: The study presents a unique microfluidic hBM-on-a-chip that accurately mimics the human bone marrow microenvironment, providing new opportunities for research and intervention in bone marrow diseases. The complex structure of the chip increases experimental efficiency and reproducibility, while offering insights into the dynamics of hematopoietic stem cells.
Review
Immunology
Yoshiki Omatsu
Summary: Throughout adult life, most blood cells including immune cells are produced from hematopoietic stem cells (HSCs) in the bone marrow. The niche for HSCs has been a subject of debate, but accumulated studies suggest that a specific population of fibroblastic reticular cells, called CAR/LepR(+) cells, are essential for maintaining HSCs and lymphoid progenitors.
INFLAMMATION AND REGENERATION
(2023)
Article
Hematology
Alanna C. Green, Gavin Tjin, Samuel C. Lee, Alistair M. Chalk, Lenny Straszkowski, Diannita Kwang, Emma K. Baker, Julie M. Quach, Takaharu Kimura, Joy Y. Wu, Louise E. Purton
Summary: The study identified four distinct skeletal cell populations in the bone marrow microenvironment, with differences in location, function, gene expression, and potential role in regulating B lymphopoiesis. One of these populations showed the highest expression of extrinsic regulation genes for B lymphopoiesis, suggesting a potential key role in hematopoiesis regulation.
Review
Engineering, Biomedical
Chandralekha Chatterjee, Peter Schertl, Miriam Frommer, Anita Ludwig-Husemann, Anna Mohra, Nadine Dilger, Toufik Naolou, Sophia Meermeyer, Timna Claire Bergmann, Alejandro Alonso Calleja, Cornelia Lee-Thedieck
Summary: Artificial niches play a crucial role in regenerative medicine research, providing insights into the biophysical and biochemical processes of hematopoietic stem cells, as well as facilitating HSCs expansion and targeted differentiation. Due to the lack of natural microenvironment, biomaterials are essential in recreating niche environments, offering promising applications in various fields.
ACTA BIOMATERIALIA
(2021)
Review
Immunology
Candice Lee Herd, Juanita Mellet, Tsungai Mashingaidze, Chrisna Durandt, Michael Sean Pepper
Summary: The dysregulation of the bone marrow niche caused by HIV infection directly and indirectly contributes to haematological abnormalities in HIV patients. The bone marrow niche, a complex multicellular environment, plays a crucial role in maintaining haematopoietic stem/progenitor cells (HSPCs). These adult stem cells are responsible for replenishing blood and immune cells throughout a lifetime. The cells in the bone marrow niche provide support for HSPCs and regulate their quiescence, self-renewal, and differentiation through chemical signals and cell-cell interactions. This review highlights the dysregulation of the bone marrow niche in HIV infection and the vulnerability of HSPCs to HIV.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Raquel S. Pereira, Rahul Kumar, Alessia Cais, Lara Paulini, Alisa Kahler, Jimena Bravo, Valentina R. Minciacchi, Theresa Krack, Eric Kowarz, Costanza Zanetti, Parimala Sonika Godavarthy, Fabian Hoeller, Pablo Llavona, Tabea Stark, Georg Tascher, Daniel Nowak, Eshwar Meduri, Brian J. P. Huntly, Christian Muench, Francesco Pampaloni, Rolf Marschalek, Daniela S. Krause
Summary: This study shows that the calcium-sensing receptor (CaSR) can influence the distribution of acute myeloid leukaemia (AML) cells in the bone marrow microenvironment, affecting the self-renewal of leukaemic stem cells and the progression of AML.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Anri Koyanagi, Iichiroh Onishi, Karin Muraoka, Ikue Sato, Shingo Sato, Tsuyoshi Kimura, Akio Kishida, Kouhei Yamamoto, Masanobu Kitagawa, Morito Kurata
Summary: Hematopoiesis is maintained by the interaction of hematopoietic stem cells and bone marrow mesenchymal stem cells in bone marrow microenvironments. Certain genetic mutations in the mesenchymal stem cells can lead to hematopoietic neoplasms. This study used a specific gene mutation in human mesenchymal stem cells and a bone scaffold to create an in vivo bone marrow microenvironment model, providing insights into these interactions and disease onset.
BIOENGINEERING-BASEL
(2022)
Meeting Abstract
Hematology
Trent Hall, Megan Walker, Miguel Ganuza, Marie Bordas, Per Holmfeldt, Shannon McKinney-Freeman
EXPERIMENTAL HEMATOLOGY
(2016)
Meeting Abstract
Hematology
Miguel Ganuza, Trent Hall, Ashley Chabot, Shannon McKinney-Freeman
EXPERIMENTAL HEMATOLOGY
(2016)
Article
Immunology
Per Holmfeldt, Miguel Ganuza, Himangi Marathe, Bing He, Trent Hall, Guolian Kang, Joseph Moen, Jennifer Pardieck, Angelica C. Saulsberry, Alba Cico, Ludovic Gaut, Daniel McGoldrick, David Finkelstein, Kai Tan, Shannon McKinney-Freeman
JOURNAL OF EXPERIMENTAL MEDICINE
(2016)
Article
Hematology
Miguel Ganuza, Brandon Hadland, Ashley Chabot, Chen Li, Guolian Kang, Irwin Bernstein, Shannon McKinney-Freeman
EXPERIMENTAL HEMATOLOGY
(2017)
Article
Cell Biology
Miguel Ganuza, Trent Hall, David Finkelstein, Ashley Chabot, Guolian Kang, Shannon McKinney-Freeman
NATURE CELL BIOLOGY
(2017)
Editorial Material
Cell Biology
Miguel Ganuza, David Santamaria
Article
Biochemistry & Molecular Biology
Miguel Ganuza, Cristina Saiz-Ladera, Marta Canamero, Gonzalo Gomez, Ralph Schneider, Maria A. Blasco, David Pisano, Jesus M. Paramio, David Santamaria, Mariano Barbacid
Meeting Abstract
Hematology
Miguel Ganuza, Per Holmfeldt, Jennifer Pardieck, Trent Hall, Shannon McKinney-Freeman
EXPERIMENTAL HEMATOLOGY
(2014)
Article
Multidisciplinary Sciences
Miguel Ganuza, Ashley Chabot, Xing Tang, Wenjian Bi, Sivaraman Natarajan, Robert Carter, Charles Gawad, Guolian Kang, Yong Cheng, Shannon McKinney-Freeman
NATURE COMMUNICATIONS
(2018)
Article
Hematology
Miguel Ganuza, Trent Hall, David Finkelstein, Yong-Dong Wang, Ashley Chabot, Guolian Kang, Wenjian Bi, Gang Wu, Shannon McKinney-Freeman
Article
Biochemistry & Molecular Biology
Myrtani Pieri, Elena Theori, Harsh Dweep, Myrofora Flourentzou, Foteini Kalampalika, Maria-Arsenia Maniori, Gregory Papagregoriou, Christos Papaneophytou, Kyriacos Felekkis
Summary: Colorectal cancer is the third most frequent human cancer globally. High consumption of red meat, including beef, is considered a risk factor for its initiation and progression. This study discovered that exogenous bovine miRNAs ingested into the body may have an effect on human cells. The research found that bovine miRNA can still be detected after cooking and digestion, but it does not affect the viability of human cell lines. Further experiments will explore whether bovine miRNA can contribute to the progression of colorectal cancer through its consumption.
Review
Hematology
Miguel Ganuza, Wilson Clements, Shannon McKinney-Freeman
Summary: The article discusses the emergence of new technologies and recent exploration of cell numbers and dynamics during development, aiming to better understand the origins of hematologic disease and cultivate HSCs. It points out that many cells lack transplantability during embryo development, but still contribute to the adult HSC pool in the end.
