Journal
NATURE CELL BIOLOGY
Volume 19, Issue 10, Pages 1153-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb3607
Keywords
-
Categories
Funding
- American Society of Hematology
- Hartwell Foundation
- NIDDK [K01DK080846, R01DK104028]
- American Lebanese Syrian Associated Charities (ALSAC)
- NCI [P30 CA021765-35]
- National Cancer Institute at the National Institute of Health [P30 CA21765]
- ALSAC
- NCI (SJCRH Cell & Tissue Imaging Center)
Ask authors/readers for more resources
Current dogma asserts that mammalian lifelong blood production is established by a small number of blood progenitors. However, this model is based on assays that require the disruption, transplantation and/or culture of embryonic tissues. Here, we used the sample-to-sample variance of a multicoloured lineage trace reporter to assess the frequency of emerging lifelong blood progenitors while avoiding the disruption, culture or transplantation of embryos. We find that approximately 719 Flk1(+) mesodermal precursors, 633 VE-cadherin(+) endothelial precursors and 545 Vav1(+) nascent blood stem and progenitor cells emerge to establish the haematopoietic system at embryonic days (E) 7-E8.5, E8.5-E11.5 and E11.5-E14.5, respectively. We also determined that the spatio-temporal recruitment of endothelial blood precursors begins at E8.5 and ends by E10.5, and that many c-Kit(+) clusters of newly specified blood progenitors in the aorta are polyclonal in origin. Our work illuminates the dynamics of the developing mammalian blood system during homeostasis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available