Article
Chemistry, Analytical
Yupan Wu, Yuanbo Yue, Haohao Zhang, Xun Ma, Kemu Li, Wen Zeng, Shaoxi Wang, Yingqi Meng
Summary: In this study, a microfluidic device based on bipolar electrode (BPE) array is developed for assessing cell viability using AC electrodynamics. The viability of various cells can be evaluated by analyzing the electro-rotation (ROT) speed and direction of cells, as well as the dielectrophoresis (DEP) responses of cells. This study demonstrates the utility of BPEs in enabling scalable and high-throughput AC electrodynamics platforms for cell characterization and viability assessment.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Shahriar Shalileh, Mohammad Ali Khayamian, Mohammadreza Ghaderinia, Hamed Abadijoo, Hamed Hassanzadeh-Moghadam, Azam Dalman, Hossein Simaee, Mahsa Faramarzpour, Pooneh Ghaznavi, Pouya Soltan Khamsi, Fereshteh Abbasvandi, Mohammad Faranoush, Robab Anbiaei, Poopak Eftekhari-Yazdi, Mohammad Abdolahad
Summary: The study found that non-cancer cells exhibit a higher electrodeformation response, while malignant cells show a weaker stretch response. Utilizing this difference, a label-free mechanoelectrical chip was proposed for detecting metastatic cancer cells, presenting a new approach for cancer diagnosis and research.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Multidisciplinary Sciences
Andre Lai, Rachel Rex, Kristen L. Cotner, Alan Dong, Michael Lustig, Lydia L. Sohn
Summary: Cellular mechanical properties are important in biological processes and diseases. Traditional methods capture rich information but have low throughput. High-throughput methods can only measure limited mechanical information. Mechano-node-pore sensing is a flexible microfluidic platform that bridges this gap by providing multi-parameter measurements with moderate throughput.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Engineering, Electrical & Electronic
Siyao Chen, Yoshihisa Yamashige, Takeshi Mitsunaka, Naoshi Kondo, Keiichiro Shiraga, Yuichi Ogawa
Summary: This paper proposes a miniature, sensitive, and label-free method for detecting Escherichia coli (E. coli) and Saccharomyces cerevisiae (yeast) using a complementary metal-oxide-semiconductor (CMOS) chip with die-lectrophoresis (DEP)-assisted LC-oscillator arrays. The DEP electrodes enhance the measurement sensitivity by collecting microorganisms at the most sensitive position of the LC-oscillator. The results show that this novel approach can achieve high-sensitivity detection of microorganisms and has the potential to be used as a rapid and low-cost lab-on-a-chip sensor for food inspection and clinical diagnosis.
SENSORS AND ACTUATORS A-PHYSICAL
(2023)
Article
Chemistry, Analytical
Yanjuan Wang, Ning Tong, Fengqi Li, Kai Zhao, Deguang Wang, Yijie Niu, Fengqiang Xu, Jiale Cheng, Junsheng Wang
Summary: In this study, a microfluidic trapping system based on AC dielectrophoresis (DEP) technology was proposed for precise trapping and release of specific microparticles. Numerical simulation and experimental verification were conducted to confirm the effectiveness of the designed system, contributing to the application of cell trapping and manipulation, as well as single-cell analysis.
Article
Biochemical Research Methods
Yang Bu, Jinhui Wang, Sheng Ni, Yusong Guo, Levent Yobas
Summary: Extracellular vesicles (EVs) are cell-derived bioparticles that have significant roles in various biological processes and can be potential biomarkers for pre-diagnostic applications. However, isolating EV subpopulations, especially exosomes, from biological fluids is challenging due to their small size. In this study, a microfluidic device combining electrothermal fluid rolls and dielectrophoresis was used for continuous-flow label-free size fractionation of EVs. The device showed high efficiency in separating submicron particles and isolating intact exosomes from cell culture medium or blood serum. This method provides a promising platform for the purification of target bioparticles directly from physiological fluids and may have important implications in EV-related diagnostic applications.
Article
Chemistry, Analytical
Md Nazibul Islam, Nicholas Mavrogiannis, Zachary Gagnon
Summary: Using fluidic dielectrophoresis, the motion of a polarizable liquid-liquid interface can be used to quantify reaction rate kinetics in solution. By controlling the electric field and frequency, the direction and magnitude of interface displacement can provide information about the binding dynamics. The measurement of the crossover frequency allows for quantitative analysis of the binding kinetics.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biophysics
Yu-Sheng Lin, Chen-li Sun, Sung Tsang, Sakina Bensalem, Bruno Le Pioufle, Hsiang -Yu Wang
Summary: This study establishes a label-free, rapid, noninvasive, and quantitative analysis of cell surface properties and intracellular contents using electrorotation. The growth phase of microalgae cells can be identified by combining this information. The measurement accuracy is satisfactory for microscale and nanoscale epistructures in different growth phases.
BIOPHYSICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Bo Yao, Yunze Yang, Nanxi Yu, Nongjian Tao, Di Wang, Shaopeng Wang, Fenni Zhang
Summary: This article introduces an optical imaging method to measure molecular interactions with live cells by tracking nanometer membrane fluctuations. The elastic properties of the cell are determined through analysis of the data, as well as the binding kinetics of glycoprotein to lectins. The method is anticipated to contribute to the understanding of cell interactions, mechanical assessment of diseases like cancer, and screening of membrane protein targeting drugs.
Article
Chemistry, Analytical
Xiaoming Chen, Shun Liu, Xu-guang Hu, Tengteng Liu, Mo Shen, Yun Peng, Sheng Hu, Yong Zhao
Summary: Manipulation of micro- and nanoscale objects using induced-charge electro-osmotic (ICEO) vortices is a promising approach for detection and sensing applications. However, the current throughput of this method is limited. To overcome this constraint, a novel method using parallel ICEO vortices is proposed, which allows for higher throughput without compromising efficiency. This method can be applied in disease diagnosis and environmental monitoring by integrating with simple and economical sensors.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yue Wu, Sivan Yakov, Afu Fu, Gilad Yossifon
Summary: This study successfully extends the manipulation and transport capabilities of electrically powered micro- and nanomotors in high-conductivity solutions. By combining magnetic field-based propulsion and navigation with dielectrophoretic manipulation, the micromotors can effectively manipulate and transport various cargos. The ability to identify apoptotic cells and transport live cells to specific regions is demonstrated. This hybrid micromotor approach has significant implications in drug delivery and single-cell analysis.
Article
Chemistry, Analytical
Peixian Li, Ye Ai
Summary: Biophysical markers of cells have shown promise in studying, characterizing, and classifying different cell types. The lack of cell sorting ability based on multivariate biophysical markers presents a challenge in isolating specific cell types. This study demonstrates label-free and high-throughput acoustic single-cell sorting activated by the characterization of multivariate biophysical phenotypes, providing a solution to the challenges of cell purification without specific molecular biomarkers.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Teng Fang, Pengbo Yuan, Chen Gong, Yueping Jiang, Yuezhou Yu, Wenhao Shang, Chan Tian, Anpei Ye
Summary: A new label-free method combining image recognition and Raman spectroscopy was reported for fast identification of rare nucleated red blood cells. Two deep-learning algorithms were used for cell morphological recognition on a low-cost computer, with YOLOv3 showing better real-time detection capability. Several NRBCs were successfully detected in maternal blood, confirming the methodological feasibility and offering a new idea for detecting rare cells in a label-free and computer-assisted way.
Review
Biotechnology & Applied Microbiology
Yanyu Chen, Zhuhao Wu, Joseph Sutlive, Ke Wu, Lu Mao, Jiabao Nie, Xing-Zhong Zhao, Feng Guo, Zi Chen, Qinqin Huang
Summary: Noninvasive prenatal diagnosis (NIPD) is a method of detecting genetic disorders related to the fetus before birth by analyzing markers in the peripheral blood of pregnant women. Fetal-nucleated red blood cells (fNRBCs) can be used as biomarkers for NIPD due to their unique ability to carry the complete genetic information of the fetus. Recent advances in NIPD technologies are focused on isolating and analyzing fNRBCs, using both conventional and novel methods such as micro-/nanomaterials and microfluidic chips. The identification and clinical diagnostic potential of fNRBCs are also discussed, along with the challenges and future directions of using fNRBCs in NIPD treatment guidelines.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Review
Materials Science, Multidisciplinary
Sanghyun Lee, Hojin Kim, Sung Yang
Summary: The separation of blood components is essential for biology, clinical diagnosis, therapeutics, and personalized medicine. Microfluidics has become a popular technology for this purpose, allowing for the separation of target cells based on their intrinsic properties. This review discusses various microfluidic-based hydrodynamic cell-separation technologies, categorizing them according to their working principles. The relative performances and features of these technologies are thoroughly discussed, along with future perspectives on commercialization and standardization for widespread use and applications.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Polymer Science
Chen Gao, Junbo Jiang, Juan Wang, Yutong Wang, Yangyang Zhang, Caihua Rao, Yue Cheng, Zhongrong Chen, Runhuai Yang, Gang Zhao
Summary: The mineralization of hydroxyapatite mineralized by acidic metal oxides in orthopedic substitutes has been extensively researched. In this study, Polycaprolactone (PCL) nanofibers with nano-tantalum oxide (Ta2O5) were successfully fabricated through electrospinning. The chemical reaction between hydrophobic scaffolds and acid oxides in the mineralization process has a significant impact on the biological effects of the scaffolds.
JOURNAL OF APPLIED POLYMER SCIENCE
(2022)
Article
Materials Science, Biomaterials
Jianbo Yao, Lingxiao Shen, Zhongrong Chen, Bing Zhang, Gang Zhao
Summary: This study explored a microencapsulation method for cryopreservation of red blood cells (RBCs). The results showed that the recovery of RBCs after microencapsulation was significantly improved, and the washing process was simplified. The microencapsulation method provides a promising, referenceable, and more practical strategy for future clinical transfusion medicine.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Materials Science, Multidisciplinary
Chao Ma, Meng Wang, Pierre Claver Uzabakiriho, Gang Zhao
Summary: A high-performance, safe, comfortable, waterproof, breathable, economical, and reliable wearable strain sensor was developed through mature electrospinning and screen-printing technology. The sensor exhibits excellent repeatability and stability, remarkable sensitivity, a broad working strain range, and an ultrafast response time. Its mechanical properties are similar to natural skin and it has unmatched biofriendliness.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Optics
Mengduo Jiang, Meng Shao, Xiao Yang, Linna He, Tao Peng, Tao Wang, Zeyu Ke, Zixin Wang, Shu Fang, Yuxin Mao, Xilin Ouyang, Gang Zhao, Jinhua Zhou
Summary: Classification of the morphology of red blood cells (RBCs) is crucial for evaluating the quality of long-term stored blood. This study combines quantitative phase imaging (QPI) technology with deep learning to automatically classify RBC morphology. The developed method shows higher accuracy and shorter processing time compared to traditional methods, especially for RBCs with more discocytes.
INTERNATIONAL JOURNAL OF OPTICS
(2022)
Article
Nanoscience & Nanotechnology
Meng Wang, Kai Wang, Chao Ma, Pierre Claver Uzabakiriho, Xi Chen, Gang Zhao
Summary: In this study, a mechanical gradient strategy was developed to fabricate high-performance stretchable electronic devices. By using polyvinyl alcohol glue to fix integrated circuits on stretchable circuits, the strain gradient during the stretching process was achieved, ensuring the stability and reliability of the devices. The flexible electronic devices fabricated by this method are highly stretchable and comfortable, making them suitable for various applications.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Yuan Cao, Tie Chang, Chao Fang, Yuanyuan Zhang, Huilan Liu, Gang Zhao
Summary: This study explores the ice regulation ability of two-dimensional MXene Ti3C2Tx in both cooling and thawing processes. The molecular mechanism of ice growth inhibition is attributed to the hydrogen bonds formed between Ti3C2Tx functional groups and ice/water molecules. The application of Ti3C2Tx in cryopreservation has shown to reduce cryodamage to stem cells and increase cell viability, indicating its potential in cell cryopreservation applications.
Article
Materials Science, Multidisciplinary
Lingxiao Shen, Xianhui Qin, Menghan Wang, Dayong Gao, Xilin Ouyang, Gang Zhao
Summary: This study presents a feasible and straightforward method for scalable cryopreservation of human red blood cells (RBCs). By improving the cooling rate and applying specific techniques, high freeze-thaw recovery rates can be achieved. The removal of cryoprotectants and the functional expression of postwash RBCs after cryopreservation are also evaluated.
ADVANCED ENGINEERING MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Pierre Claver Uzabakiriho, Meng Wang, Kai Wang, Chao Ma, Gang Zhao
Summary: The research team has successfully produced a high-performance, highly stretchable conductive yarn, which has potential applications in strain sensors, charging mobile phones, monitoring human activities, etc.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Multidisciplinary Sciences
Conghui Tian, Lingxiao Shen, Chenjia Gong, Yunxia Cao, Qinghua Shi, Gang Zhao
Summary: This study achieved low-concentration cryopreservation of preantral follicles by using microencapsulation and nanowarming. The research found that this method improved the viability of preantral follicles and maintained their ability to form cavities, compared to conventional water bath warming. Furthermore, it showed no significant impact on in vitro fertilization and embryo transfer.
NATURE COMMUNICATIONS
(2022)
Retraction
Chemistry, Multidisciplinary
Hai Wang, Pranay Agarwal, Gang Zhao, Guang Ji, Christopher M. Jewell, John P. Fisher, Xiongbin Lu, Xiaoming He
ACS CENTRAL SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Liyuan Zhang, Mengqiao Chi, Yue Cheng, Zhongrong Chen, Yunxia Cao, Gang Zhao
Summary: This study proposes a new method to improve the antioxidant capacity of whole ovaries during cryopreservation, static magnetic field assisted thawing. The results show that the antioxidant capacity of ovarian tissue was significantly improved by static magnetic field treatment. In addition, successful vascular regeneration and follicular development were achieved through ovarian tissue allograft transplantation.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Review
Cell & Tissue Engineering
Xiaohu Wang, Enyu Wang, Gang Zhao
Summary: This review provides an overview of the background and basic principles of cryopreservation in the field of stem cells. It discusses the main bioengineering technologies and strategies used in cryopreservation, as well as the current challenges and future prospects.
Article
Chemistry, Multidisciplinary
Pierre Claver Uzabakiriho, Meng Wang, Chao Ma, Gang Zhao
Summary: This study proposes an ultra-stretchable, breathable, and highly sensitive flexible capacitive tactile sensor and triboelectric effect core-shell nanofibers. The sensor exhibits high sensitivity, rapid response and recovery time, and excellent stability, making it suitable for various applications.
Article
Biotechnology & Applied Microbiology
Faryal Farooq Cheepa, Huilan Liu, Gang Zhao
Summary: Honey, with its antimicrobial activity, dehydrating and preserving properties, has proven to be an effective natural cryoprotectant for fertility cryopreservation, improving post-thaw quality and viability. Its increasing viscosity at low temperature provides a protective barrier to cells by reducing ice formation, and its antioxidant property protects cells from thermal damage by reducing reactive oxygen species (ROS).
BIOENGINEERING-BASEL
(2022)
