Review
Biochemical Research Methods
Juan Aspromonte, Steven Mascrez, Damien Eggermont, Giorgia Purcaro
Summary: This review focuses on the applications of solid-phase microextraction and comprehensive multidimensional gas chromatography in food analysis, emphasizing the importance of proper data treatment.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Carin von Muhlen, Luciana N. R. Mangelli, Philip J. Marriott
Summary: The concept of average theoretical peak time (ATPT) is proposed as a metric for separation speed, dividing into five levels: normal speed, high speed, very high speed, hyper speed, and ultra-high speed chromatography. This metric demonstrates robustness in evaluating chromatographic separations under different techniques and analytical conditions, and is also applied as a method development metric tool.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Jiaqi Gao, Zhongqiu Hua, Shu Xu, Hao Wan, Zinan Zhi, Xinyi Chen, Shurui Fan
Summary: An amperometric gas sensor using room temperature ionic liquid electrolyte and screen-printed electrodes was developed in this study. The sensor demonstrated rapid response speed, high sensitivity, and wide operating temperature range, making it suitable for use in extreme environments.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemical Research Methods
Michelle A. Corbally, Nicholas S. Hinz, Chris E. Freye
Summary: Gas chromatography (GC) analysis of thermally labile and high-boiling point compounds is a challenge. Low-pressure GC, utilizing vacuum from mass spectrometer and wide-bore columns, allows for elution at lower temperatures. This study compared conventional GC x GC-TOFMS and low-pressure GC x GC-TOFMS configurations for analyzing a pesticide standard mixture, investigating elution temperatures, sensitivity, and peak capacity.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Gregory Bauwens, Chiara Conchione, Nicola Sdrigotti, Sabrina Moret, Giorgia Purcaro
Summary: This study analyzed seven commercial feeds and found consistent results between two laboratories. The combination of liquid-gas chromatography and liquid chromatography coupled with comprehensive multidimensional gas chromatography, along with parallel detection using flame ionization detector and time-of-flight mass spectrometer, allowed for a more comprehensive characterization of the contaminants.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Alessia Arena, Mariosimone Zoccali, Luigi Mondello, Peter Q. Tranchida
Summary: The goal of this research was to develop a method using miniaturized solvent extraction and cryogenic-modulation comprehensive two-dimensional gas chromatography-tandem mass spectrometry to determine seventy pesticides in extra virgin olive oil. The method showed high specificity and sensitivity, with quantification limits below European legislation residue limits.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Barbara Giocastro, Mariosimone Zoccali, Peter Q. Tranchida, Luigi Mondello
Summary: The research evaluates the modulation performance of a solid-state-modulator with different modulation capillary geometries, finding that the characteristics of the capillaries have a significant impact on comprehensive two-dimensional gas chromatography separation. Specifically, the use of a 0.18 mm ID modulation column is recommended over a 0.25 mm ID one based on the results obtained.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Biochemical Research Methods
Azam Gholizadeh, Mustahsin Chowdhury, Masoud Agah
Summary: This study investigates the impact of stationary phase coating on the separation efficiency of gas chromatography microcolumns, finding that higher temperature treatment can improve efficiency but may reduce peak symmetry, with the thermally treated stationary phase at 240 degrees Celsius demonstrating significantly higher efficiency and resolution.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Karina Ramos, Alicia Riddell, Helen Tsiagras, Amber M. Hupp
Summary: This study compares the applications of ultrafast gas chromatography (UFGC) and traditional gas chromatography (GC) in the evaluation of biodiesel-diesel blended fuels. The results show that UFGC is an effective and fast technique, comparable to traditional GC.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Environmental Sciences
Pranita Panda, Rajesh Kumar Mahanta, Suchitra Mohanty, Rita Paikaray, Smruti Prava Das
Summary: Non-thermal plasma technology, specifically dielectric barrier discharge (DBD) plasma, has been used to evaluate the feasibility of degrading VOCs such as toluene, benzene, and m-xylene in atmospheric pressure conditions. By analyzing post-plasma-treated by-products and studying degradation pathways, it was found that the degradation of VOCs to CO and CO2 was successful without the use of any catalyst, with higher degradation rates than expected. These findings could be valuable for VOC abatement using non-thermal plasma technology.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Energy & Fuels
Mustahsin Chowdhury, Azam Gholizadeh, Masoud Agah
Summary: This paper presents a technique based on micro gas chromatography for fast and accurate analysis of diesel fuels adulterated with kerosene. The separation columns fabricated using MEMS technology can discriminate as little as 5% added kerosene with only four seconds of chromatogram analysis. The linear relationship between the chromatograms and diesel purity was determined through chemometrics methods.
Article
Chemistry, Applied
Subhashree Singh, Suprava Sahoo, Asit Ray, Ambika Sahoo, Sanghamitra Nayak, Basudeba Kar
Summary: The study successfully identified 102 compounds in the essential oil of Alpinia galanga using a thermal desorption modulator, providing important data for further research on its aromatic constituents.
NATURAL PRODUCT RESEARCH
(2021)
Review
Chemistry, Multidisciplinary
Noor Abdulhussain, Suhas Nawada, Peter Schoenmakers
Summary: This review focuses on the importance of separation and characterization of complex mixtures, particularly in the context of online three-dimensional separation systems. Various combinations are discussed to provide detailed information on complex samples and the need for higher peak capacities. Overall, the review highlights the potential performance, advantages, perspectives, and results obtained from these systems in the past decade.
Article
Chemistry, Analytical
Ryosuke Shiraki, Kengo Wakigawa, Shin Ogawa, Akinaga Gohda, Takeshi Mori, Yoshiki Katayama
Summary: This study investigated the use of ethenesulfonyl fluoride (ESF) as a selective reagent for derivatization of ammonia in aqueous media, aiming to develop a rapid identification method. The optimized method showed low detection limit and good linearity for aqueous ammonia, and it was able to quantify ammonia in samples containing interfering substances.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Timothy J. Trinklein, Jiaxin Jiang, Robert E. Synovec
Summary: This study presents an analytical workflow for analyzing olefins in gasoline using selective bromination and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GCxGC-TOFMS) coupled with discovery-based analysis. The workflow successfully detects and identifies olefinic compounds in gasoline by combining bromination chemistry and GCxGC separation techniques.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Xuelian Xiong, Henry Kuang, Sahar Ansari, Tongyu Liu, Jianke Gong, Shuai Wang, Xu-Yun Zhao, Yewei Ji, Chuan Li, Liang Guo, Linkang Zhou, Zhimin Chen, Paola Leon-Mimila, Meng Ting Chung, Katsuo Kurabayashi, Judy Opp, Francisco Campos-Perez, Hugo Villamil-Ramirez, Samuel Canizales-Quinteros, Robert Lyons, Carey N. Lumeng, Beiyan Zhou, Ling Qi, Adriana Huertas-Vazquez, Aldons J. Lusis, X. Z. Shawn Xu, Siming Li, Yonghao Yu, Jun Z. Li, Jiandie D. Lin
Article
Biochemical Research Methods
Maxwell Wei-Hao Li, Hongbo Zhu, Menglian Zhou, Jinyan She, Ziqi Li, Katsuo Kurabayashi, Xudong Fan
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Biochemical Research Methods
Maxwell Wei-Hao Li, Xiaolu Huang, Hongbo Zhu, Katsuo Kurabayashi, Xudong Fan
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Chemistry, Multidisciplinary
Younggeun Park, Byunghoon Ryu, Qiufang Deng, Baihong Pan, Yujing Song, Yuzi Tian, Hasan B. Alam, Yongqing Li, Xiaogan Liang, Katsuo Kurabayashi
Article
Engineering, Biomedical
Lenar Yessayan, Balazs Szamosfalvi, Lena Napolitano, Benjamin Singer, Katsuo Kurabayashi, Yujing Song, Angela Westover, H. David Humes
Article
Microbiology
Fuqiang Ma, Tianjie Guo, Yifan Zhang, Xue Bai, Changlong Li, Zelin Lu, Xi Deng, Daixi Li, Katsuo Kurabayashi, Guang-yu Yang
Summary: The study introduces a novel ultrahigh-throughput screening platform based on flow cytometric droplet sorting, which efficiently isolates new biocatalysts from metagenomic libraries and has the potential to significantly improve efficiency in exploring novel enzymes from nature.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Ryan C. Blase, Mark J. Libardoni, Gregory P. Miller, Kelly E. Miller, Charity M. Phillips-Lander, J. Hunter Waite, Christopher R. Glein, Hongbo Zhu, Abhishek Ghosh, Anandram Venkatasubramanian, Xudong Fan, Katsuo Kurabayashi
ACS EARTH AND SPACE CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Tatyana E. Saleski, Meng Ting Chung, David N. Carruthers, Azzaya Khasbaatar, Katsuo Kurabayashi, Xiaoxia Nina Lin
Summary: The method presented in the study achieved tuning of pathway gene expression levels through random integration and high-throughput screening, leading to high productivity and yield of isobutanol in Escherichia coli. Examination of pathway expression in top-performing isolates highlighted the complexity of cellular metabolism and regulation, emphasizing the importance of precise optimization during integration of pathway genes into the chromosome.
Article
Biophysics
Yujing Song, Jingyang Zhao, Tao Cai, Andrew Stephens, Shiuan-Haur Su, Erin Sandford, Christopher Flora, Benjamin H. Singer, Monalisa Ghosh, Sung Won Choi, Muneesh Tewari, Katsuo Kurabayashi
Summary: The study introduces a rapid, accurate, and highly multiplexed microfluidic digital immunoassay incorporating machine learning-based autonomous image analysis, enabling the detection of multiple biomarkers in a short period of time. The method offers a promising pathway to transform critical care of acutely ill patients, utilizing advanced technology to monitor immune status and cytokine storm syndrome in real-time.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemical Research Methods
Yujing Song, Yuxuan Ye, Shiuan-Haur Su, Andrew Stephens, Tao Cai, Meng-Ting Chung, Meilan K. Han, Michael W. Newstead, Lenar Yessayan, David Frame, H. David Humes, Benjamin H. Singer, Katsuo Kurabayashi
Summary: The study introduces a digital protein microarray platform for rapid cytokine monitoring in critically ill COVID-19 patients, demonstrating high sensitivity and rapid operation. By monitoring cytokine levels continuously, it helps guide personalized treatment strategies in response to the large variability in patients' reactions to COVID-19.
Article
Chemistry, Analytical
Byunghoon Ryu, Jay Chen, Katsuo Kurabayashi, Xiaogan Liang, Younggeun Park
Proceedings Paper
Engineering, Electrical & Electronic
Younggeun Park, Byunghoon Ryu, Xiaogan Liang, Katsuo Kurabayashi
PROCEEDINGS OF 2019 6TH INTERNATIONAL WORKSHOP ON LOW TEMPERATURE BONDING FOR 3D INTEGRATION (LTB-3D)
(2019)
Article
Biochemical Research Methods
Andrew Stephens, Robert Nidetz, Nicolas Mesyngier, Meng Ting Chung, Yujing Song, Jianping Fu, Katsuo Kurabayashi
Article
Biochemical Research Methods
Meng Ting Chung, Katsuo Kurabayashi, Dawen Cai
Meeting Abstract
Rheumatology
Yasushi Kondo, Yoko Nakagome, Kazuki Hirano, Yasushi Koyama, Sho Sasaki, Katayoshi Kurabayashi, Yuto Izumi, Chiho Yamada, Shinji Sato
ANNALS OF THE RHEUMATIC DISEASES
(2019)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)