Optimized gene expression from bacterial chromosome by high-throughput integration and screening

High-Resolution Mapping of the Escherichia coli Chromosome Reveals Positions of High and Low Transcription

(2019) Scott A. Scholz et al.   Cell Systems

Synthetic biology approaches for chromosomal integration of genes and pathways in industrial microbial systems

(2019) Lei Li et al.   BIOTECHNOLOGY ADVANCES

Syntrophic co-culture amplification of production phenotype for high-throughput screening of microbial strain libraries

(2019) Tatyana E. Saleski et al.   METABOLIC ENGINEERING

Techniques for chromosomal integration and expression optimization in Escherichia coli

(2018) Bing-ming Ou et al.   BIOTECHNOLOGY AND BIOENGINEERING

OptSSeq explores enzyme expression and function landscapes to maximize isobutanol production rate

(2018) Indro N. Ghosh et al.   METABOLIC ENGINEERING

Controlling and exploiting cell-to-cell variation in metabolic engineering

(2018) Tiebin Wang et al.   CURRENT OPINION IN BIOTECHNOLOGY

Engineering Cellular Metabolism

(2016) Jens Nielsen et al.   CELL

Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR

(2016) Michael Jahn et al.   Microbial Cell Factories

Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli

(2016) Marcelo C. Bassalo et al.   ACS Synthetic Biology

Polar Fixation of Plasmids during Recombinant Protein Production in Bacillus megaterium Results in Population Heterogeneity

(2015) Karin M. Münch et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Effects of chromosomal gene copy number and locations on polyhydroxyalkanoate synthesis by Escherichia coli and Halomonas sp.

(2015) Jin Yin et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Bacterial production of isobutanol without expensive reagents

(2014) Hironaga Akita et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Chromosome position effects on gene expression in Escherichia coli K-12

(2014) Jack A. Bryant et al.   NUCLEIC ACIDS RESEARCH

Comparative Transcription Profiling and In-Depth Characterization of Plasmid-Based and Plasmid-Free Escherichia coli Expression Systems under Production Conditions

(2013) Juergen Mairhofer et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Metabolic Engineering: Past and Future

(2013) Benjamin M. Woolston et al.   Annual Review of Chemical and Biomolecular Engineering

TREX: A Universal Tool for the Transfer and Expression of Biosynthetic Pathways in Bacteria

(2012) Anita Loeschcke et al.   ACS Synthetic Biology

High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal

(2011) Antonino Baez et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Effects of a recombinant gene expression on ColE1-like plasmid segregation in Escherichia coli

(2011) Mladen Popov et al.   BMC BIOTECHNOLOGY

Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR

(2011) Kang Zhou et al.   BMC MOLECULAR BIOLOGY

Gene replacement techniques for Escherichia coli genome modification

(2011) Mahesh Madyagol et al.   FOLIA MICROBIOLOGICA

Multicopy plasmid stability: Revisiting the dimer catastrophe

(2011) C.M. Field et al.   JOURNAL OF THEORETICAL BIOLOGY

An evolutionary strategy for isobutanol production strain development in Escherichia coli

(2011) Kevin M. Smith et al.   METABOLIC ENGINEERING

Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli

(2011) Sabine Bastian et al.   METABOLIC ENGINEERING

Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/alcohol dehydrogenase genes

(2009) Shota Atsumi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Plasmid-free T7-basedEscherichia coliexpression systems

(2009) Gerald Striedner et al.   BIOTECHNOLOGY AND BIOENGINEERING

Correlation of mRNA and protein in complex biological samples

(2009) Tobias Maier et al.   FEBS LETTERS

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

(2009) Jean-Christophe Baret et al.   LAB ON A CHIP

Metabolic engineering without plasmids

(2009) Aashiq H Kachroo et al.   NATURE BIOTECHNOLOGY

Stabilized gene duplication enables long-term selection-free heterologous pathway expression

(2009) Keith E J Tyo et al.   NATURE BIOTECHNOLOGY

Dual-In/Out strategy for genes integration into bacterial chromosome: a novel approach to step-by-step construction of plasmid-less marker-less recombinant E. coli strains with predesigned genome structure

(2008) Natalia I Minaeva et al.   BMC BIOTECHNOLOGY

Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels

(2008) Shota Atsumi et al.   NATURE