Article
Anatomy & Morphology
Heitor Hussni Casarin, Vicente Silva Mattos, Jarbas Caiado de Castro Neto, Michelle Alexandra Chinelatti
Summary: The study evaluated the chemical and morphological changes of femtosecond laser-irradiated enamel using Raman spectroscopy, CLSM, and SEM. Results showed a significant decrease in carbonate content and carbonate/phosphate ratio in enamel after laser irradiation, without altering its structure and morphology.
MICROSCOPY RESEARCH AND TECHNIQUE
(2021)
Article
Agronomy
Robert Lufu, Alemayehu Ambaw, Umezuruike Linus Opara
Summary: The study found that pomegranate fruits are prone to moisture loss during storage, leading to compromised visual appearance and financial loss. Factors contributing to water loss include increased count of lenticels, widening of micro-cracks, and fragmentation of waxy cuticle. Differences in peel tissue thickness and structure at different parts of the fruit also affect water loss trends.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Svetlana Yefimova, Anatolii Onishchenko, Vladimir Klochkov, Valeriy Myasoedov, Yurii Kot, Liliya Tryfonyuk, Oleksandr Knigavko, Pavel Maksimchuk, Umut Kokbas, Yuliia Kalashnyk-Vakulenko, Andrii Arkatov, Vladyslav Khanzhyn, Volodymyr Prokopyuk, Iryna Vyshnytska, Anton Tkachenko
Summary: Rare-earth orthovanadate nanoparticles (ReVO4:Eu3+, Re = Gd, Y or La) have potential as agents for photodynamic therapy of cancer due to their modifiable redox properties. However, their toxicity limits their application. This research aimed to study the pro-eryptotic effects of GdVO4:Eu3+ and LaVO4:Eu3+ nanoparticles and identify the mechanisms of eryptosis induction.
Article
Optics
Jakob G. P. Lingg, Thomas S. Bischof, Bernardo A. Arus, Emily D. Cosco, Ellen M. Sletten, Christopher J. Rowlands, Oliver T. Bruns, Andriy Chmyrov
Summary: The development of fluorophores with photoemission beyond 1000 nm provides the opportunity to develop novel fluorescence microscopes sensitive to those wavelengths. Imaging at wavelengths beyond the visible spectrum enables imaging depths of hundreds of microns in intact tissue, making this attractive for volumetric imaging applications.
LASER & PHOTONICS REVIEWS
(2023)
Article
Medicine, Research & Experimental
Shabeeba M. Ashraf, Susobhan Mahanty, Krishnan Rathinasamy
Summary: Securinine effectively inhibits the proliferation of cervical, breast, and lung cancer cells while inducing minimal toxicity in non-malignant HEK cells. It disrupts microtubule organization in both interphase and mitotic phases, as well as inhibits tubulin assembly. Additionally, securinine induces a mitochondrial-dependent ROS response in cancer cells and leads to apoptosis through a p53-dependent pathway in MCF-7 cells.
Review
Immunology
Ryley D. McClelland, Tyce N. Culp, David J. Marchant
Summary: Viruses use host factors to enter cells and cause disease, and imaging techniques are crucial for studying virus entry and replication processes, providing insights into viral infection mechanisms. Quantifying viral images and using cryo-electron microscopy to gather structural information of viruses can help identify novel targets for antiviral therapeutics and vaccines.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Mojgan Ebadi, Mohammad Reza Zolfaghari, Seyyed Soheil Aghaei, Mohsen Zargar, Kambiz Akbari Noghabi
Summary: Using the cell extract of a newly characterized cyanobacterial strain Desertifilum sp. EAZ03 for biosynthesis of ZnO NPs showed promising antimicrobial, antibiofilm, and anticancer activities, highlighting the potential of microbial systems in green synthesis of metal oxide nanoparticles.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Food Science & Technology
Liliana Perez-Lavalle, Antonio Valero, Manuel Cejudo-Gomez, Elena Carrasco
Summary: Fresh strawberries are popular and consumed frequently, but outbreaks caused by food-borne pathogens have been associated with their consumption. Salmonella enterica subsp. enterica serovar Thompson can form biofilms on the surface of strawberries during storage, which allows the bacteria to survive and spread. This is crucial for food safety.
Review
Immunology
Raffaella Morini, Matteo Bizzotto, Fabio Perrucci, Fabia Filipello, Michela Matteoli
Summary: The role of microglia in controlling synapse homeostasis is increasingly recognized by the scientific community, with potential therapeutic implications in conditions like schizophrenia and Alzheimer's Disease. Methodological approaches to investigate microglial synaptic engulfment include various in vitro assays and co-cultures of microglia and neurons, requiring further validation in mice brain for representative in vivo results. This review aims to analyze the technical approaches to studying microglia-mediated phagocytosis of neuronal and synaptic substrates in critical developmental time windows.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Environmental Sciences
Dantong Liu, Yuhan Zheng, Lyujun Chen, Donghui Wen
Summary: Due to limitations in current detection technologies, the distribution and characteristics of small-sized microplastics in coastal sediment remain uncertain. In this study, a new method based on micro-Raman spectroscopy, called multipoint confocal micro-Raman spectrum scanning (MCmRSS), was established to detect small-sized microplastics. The MCmRSS was successfully applied to detect microplastics in sediment samples collected from three bays of the East China Sea. The findings showed that the small-sized microplastics accounted for 67% of all microplastic items, and the concentration of microplastics followed a power-exponential equation in relation to their size.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Mechanical
M. Gaier, H-T Lin, Z. N. Farhat, K. P. Plucknett
Summary: This study demonstrates the feasibility of using a heat treatable binder phase for TiC-based cermets, showing a significant increase in hardness and scratch resistance with heat treatment. The research provides insights into the effects of heat treatments on stainless steels in metal ceramic composites.
Article
Plant Sciences
Justyna Stefanowicz-Hajduk, Magdalena Gucwa, Barbara Moniuszko-Szajwaj, Anna Stochmal, Anna Kawiak, J. Renata Ochocka
Summary: The bufadienolide compound bersaldegenin-1,3,5-orthoacetate exhibits strong cytotoxicity against cervical cancer cells by inducing cell cycle arrest and caspase-independent cell death through double-stranded DNA damage and overproduction of reactive oxygen species. The compound also leads to the overexpression of NF-Kappa-B inhibitor genes in the treated cells. These findings provide important insights for further research on cell death signaling pathways induced by bufadienolides.
PHARMACEUTICAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Victory Armida Janine Jaques, Eva Zikmundova, Jiri Holas, Tomas Zikmund, Jozef Kaiser, Katarina Holcova
Summary: Scanning electron microscopy (SEM) is a commonly used method for analyzing painting micro-samples with high resolution and precise surface analysis. In this field, low-vacuum SEM (LV-SEM) is mostly used, and this work presents two conductive cross-section preparation methods for non-conductive samples to reduce charging effects without impairing sample integrity.
SCIENTIFIC REPORTS
(2022)
Review
Physiology
Andrea M. Lopez-Canizales, Aracely Angulo-Molina, Adriana Garibay-Escobar, Erika Silva-Campa, Miguel A. Mendez-Rojas, Karla Santacruz-Gomez, Monica Acosta-Elias, Beatriz Castaneda-Medina, Diego Soto-Puebla, Osiris Alvarez-Bajo, Alexel Burgara-Estrella, Martin Pedroza-Montero
Summary: This article discusses the changes in the membrane of red blood cells caused by storage and irradiation, characterized using various techniques. The experimental results serve as biomarkers of cell aging and storage damage.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hui Ling Ma, Laudemir Carlos Varanda, Janice Rodrigues Perussi, Emanuel Carrilho
Summary: In this study, Hypericin-loaded oil-in-water nanoemulsions (NEs) were successfully produced to enhance the solubility and bioavailability of Hypericin. The physical and biological properties of the NEs were characterized, showing significant cytotoxic effects against breast cancer cells. The results suggest that the Hypericin-loaded NEs could be a promising approach for enhancing the efficacy of Hypericin in cancer treatment.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Food Science & Technology
Cristina Fuentes, Ana Fuentes, Hugh J. Byrne, Jose Manuel Barat, Maria Jose Ruiz
Summary: The cytotoxicity of carvacrol and thymol-functionalised mesoporous silica microparticles was evaluated in human hepatocarcinoma cells, which showed that the materials induced toxicity by causing oxidative stress and resulting in apoptosis.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Spectroscopy
Isha Behl, Genecy Calado, Anika Vishwakarma, Damien Traynor, Stephen Flint, Sheila Galvin, Claire M. Healy, Marina Leite Pimentel, Alison Malkin, Hugh J. Byrne, Fiona M. Lyng
Summary: Raman microspectroscopy has demonstrated potential for oral cancer diagnosis by effectively distinguishing healthy donors from patients with oral mucosal dysplastic lesions. Models based on cytopathological and histopathological assessment can help differentiate low and high grade dysplasia, potentially reducing the need for multiple biopsies.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Correction
Chemistry, Analytical
David Perez-Guaita, Guillermo Quintas, Zeineb Farhane, Roma Tauler, Hugh J. Byrne
Article
Biochemistry & Molecular Biology
Hichem Kichou, Emilie Munnier, Yuri Dancik, Kamilia Kemel, Hugh J. Byrne, Ali Tfayli, Dominique Bertrand, Martin Souce, Igor Chourpa, Franck Bonnier
Summary: Confocal Raman microscopy (CRM) coupled with multivariate analysis was used to quantitatively measure active ingredients applied to ex vivo human stratum corneum. The method demonstrated reliability and accuracy by establishing key performance indicators and comparing them to established methodologies. Numerical criteria for analytical performance were determined, and Raman spectroscopy was shown to comply with international guidelines, making it a valuable tool for permeation studies.
Article
Cell Biology
David Perez-Guaita, Guillermo Quintas, Zeineb Farhane, Roma Tauler, Hugh J. Byrne
Summary: Raman microspectroscopy is a label-free technique that is well-suited for studying drug uptake and cellular responses. This paper proposes the integration of kinetic equations into the modeling process to provide a more comprehensive understanding of the system. The results show that the choice of equations and the temporal resolution of the data are crucial factors, and the use of tailored kinetic constraints can improve the accuracy of the results.
Article
Marine & Freshwater Biology
Gabriela H. Da Silva, Lidiane Silva Franqui, Marcelo A. De Farias, Vera Lucia S. S. De Castro, Hugh J. Byrne, Diego S. T. Martinez, Regina T. R. Monteiro, Alan Casey
Summary: This study investigated the cytotoxicity, protein corona formation, and cellular internalisation of TiO2_MWCNT on RTG-2 cells for the first time. The results showed that this nanohybrid had no toxicity effects on RTG-2 cells within 24 hours. Spectral and microscopic analysis revealed that TiO2_MWCNT can form complexes through adsorption of proteins and be internalised by cells.
AQUATIC TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shane Forde, Giulianna Vozza, David J. Brayden, Hugh J. Byrne, Jesus M. Frias, Sinead M. Ryan
Summary: In order to address the challenges of oral administration of SeMet, researchers encapsulated it in zein-coated nanoparticles made from chitosan using an ionic gelation formulation. The SeMet-NPs showed high stability and minimal cytotoxicity, as well as antioxidant properties. Additionally, they exhibited increased intestinal permeability compared to free SeMet. These findings suggest the potential of SeMet-NPs for further development as an oral supplement.
Article
Chemistry, Multidisciplinary
Furong Tian, Luis Felipe das Chagas e Silva de Carvalho, Alan Casey, Marcelo Saito Nogueira, Hugh J. Byrne
Summary: In recent years, the disease burden of hyperuricemia has been increasing, especially in high-income countries and the economically developing world with a Western lifestyle. Improved methods of uric acid and hypoxanthine detection using surface-enhanced Raman spectroscopy (SERS) and high-performance liquid chromatography (HPLC) were demonstrated by analyzing three different bodily fluids. Gold nanostars were used for quantitative detection of uric acid and hypoxanthine concentrations. The results show strong correlations between HPLC and SERS, and the potential of vibrational spectroscopy in clinical applications was highlighted.
Article
Pharmacology & Pharmacy
Suha Elderderi, Franck Bonnier, Xavier Perse, Hugh J. Byrne, Florent Yvergnaux, Igor Chourpa, Abdalla A. Elbashir, Emilie Munnier
Summary: Raman spectroscopy is a useful technique for quantifying complex cosmetic products without the need for extensive pre-analytical processing. This study demonstrates the effectiveness of Raman spectroscopy coupled with partial least squares regression (PLSR) in quantifying Alginate nanoencapsulated Piperonyl Esters (ANC-PE) in a hydrogel. By dividing the samples into a training set and a test set, the accuracy of the prediction model was evaluated, with a root mean square error of cross-validation (RMSECV) and prediction (RMSEP) of 0.142% and 0.148% (w/w PE), respectively. These results highlight the potential of Raman spectroscopy for rapid and label-free quantification of active cosmetic ingredients in complex formulations, promising future applications in analytical quality control (AQC).
Article
Chemistry, Analytical
Maria Joao Oliveira, Soraia Caetano, Ana Dalot, Filipe Sabino, Tomas R. Calmeiro, Elvira Fortunato, Rodrigo Martins, Eulalia Pereira, Miguel Prudencio, Hugh J. Byrne, Ricardo Franco, Hugo Aguas
Summary: Early and accurate detection of pathogenic microorganisms is crucial for diagnosis and patient outcomes. The combination of a polystyrene-based microfluidic device and Surface-Enhanced Raman Spectroscopy shows excellent sensitivity and specificity in detecting malaria. The system can be easily adapted for other pathogens and has the potential for early diagnosis of infectious diseases.
Article
Chemistry, Analytical
Valentina Notarstefano, Alessia Belloni, Paolo Mariani, Giulia Orilisi, Giovanna Orsini, Elisabetta Giorgini, Hugh J. J. Byrne
Summary: In this study, Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) and Principal Component Analysis (PCA) were used to analyze Raman MicroSpectroscopy (RMS) data of Oral Squamous Carcinoma Cells (OSCC) exposed to the chemotherapeutic agent cisplatin. The results showed that RMS coupled with multivariate analysis can quantitatively monitor the progression of cellular responses to different drugs, providing potential applications for label-free, in vitro, pre-clinical screening.
Review
Endocrinology & Metabolism
Zohreh Mirveis, Orla Howe, Paul Cahill, Nitin Patil, Hugh J. J. Byrne
Summary: In recent years, the analysis of the glutamine metabolic pathway has been a major focus in metabolomics research due to its significance in cell biosynthesis and bioenergetics, especially in cancer cell survival. However, monitoring the kinetics of this pathway in a living cell in real-time and holistically remains a challenge.
Article
Materials Science, Multidisciplinary
Aimee Sheehan, Tatsiana Mikulchyk, Catherine S. P. De Castro, Safakath Karuthedath, Wejdan Althobaiti, Metodej Dvoracek, Hugh J. Sabad-e-Gul, Hugh J. Byrne, Frederic Laquai, Izabela Naydenova, Mikhail A. Filatov
Summary: A series of new heavy-atom-free photosensitizers were designed and applied for holographic recording in a photopolymerizable material. The photoinduced electron transfer and spin-orbit charge transfer intersystem crossing were studied, and highly efficient photosensitization was observed.
JOURNAL OF MATERIALS CHEMISTRY C
(2023)
Article
Chemistry, Analytical
Suha Elderderi, Soukaina Hilali, Laura Wils, Igor Chourpa, Martin Souce, Barbara Clement-Larosiere, Abdalla A. Elbashir, Hugh J. Byrne, Emilie Munnier, Leslie Boudesocque-Delaye, Franck Bonnier
Summary: The study evaluated the feasibility of using ATR-IR and PLSR as a rapid, label-free, and cost-effective tool to quantify water content in extracts from spirulina wet biomass obtained using NADES. The results highlighted the accuracy and reliability of the technique in quantitative analysis, offering powerful alternatives for developing more environmentally responsible analytical protocols.
ANALYTICAL METHODS
(2022)