Article
Agriculture, Multidisciplinary
Jie Cheng, Juan Li, Linggang Zheng
Summary: 1,4-Butanediol is a significant commodity chemical currently produced through energy-intensive processes with severe environmental impacts. As market demand increases, attention has shifted towards sustainable bioproduction methods, leading to advancements in metabolic engineering strategies.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemical Research Methods
Yu Liu, Xuecong Cen, Dehua Liu, Zhen Chen
Summary: Efforts to improve the production efficiency of (R)-1,3-BDO by Escherichia coli through different metabolic engineering strategies have successfully optimized key pathway enzymes, increased NADPH supply, optimized fermentation conditions, and reduced byproducts formation. The best engineered strain can efficiently produce (R)-1,3-BDO with a yield of 0.6 mol/mol glucose, corresponding to 60% of the theoretical yield. Additionally, the feasibility of aerobically producing 1,3-BDO via a new pathway using 3-hydroxybutyrate as an intermediate has been demonstrated.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Agricultural Engineering
Tayyab Islam, Thuan Phu Nguyen-Vo, Vivek Kumar Gaur, Junhak Lee, Sunghoon Park
Summary: The production of 1,3-butanediol from glucose was achieved by engineering a pathway in Escherichia coli and optimizing gene expression levels and culture conditions. The highest ever reported titer of 1,3-butanediol in biological production methods was achieved at 257 mM in 36 hours with a yield of 0.51 mol/mol. This study provides a promising strategy for sustainable synthesis of other acetyl-CoA-derived chemicals using microbial cell factories.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Tayyab Islam, Thuan Phu Nguyen-Vo, Seunghyun Cho, Junhak Lee, Vivek Kumar Gaur, Sunghoon Park
Summary: This study focuses on metabolic engineering of Escherichia coli to efficiently produce 1,3-BDO. By reducing the accumulation of intermediate metabolites and byproducts and improving NADPH availability, a record-high titer of optically pure (R)-1,3-BDO was achieved.
BIORESOURCE TECHNOLOGY
(2023)
Article
Microbiology
Parsa Ghadermazi, Angela Re, Luca Ricci, Siu Hung Joshua Chan
Summary: Gas fermentation is a promising method to convert low-cost waste gases into valuable chemicals. This study used modeling and kinetic ensemble modeling to analyze metabolic engineering interventions for increased 2,3-butanediol production and reduced carbon loss. The results suggested that interventions targeting by-products branching from acetyl-CoA and pyruvate are effective strategies.
Article
Biotechnology & Applied Microbiology
Mengyun Kou, Zhenzhen Cui, Jing Fu, Wei Dai, Zhiwen Wang, Tao Chen
Summary: In this study, a C. glutamicum strain was engineered to efficiently produce optically pure (2R,3R)-2,3-butanediol. Through genetic modification and fermentation engineering, the titer of (2R,3R)-2,3-butanediol reached a record-high level, making C. glutamicum a competitive candidate for commercial production of this compound.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Hai-De Wang, Jian-Zhong Xu, Wei-Guo Zhang
Summary: In this study, a high L-arginine (L-Arg) producing E. coli strain was obtained through metabolic engineering. The genes speC and speF were found to play important roles in L-Arg accumulation. Feedback inhibition of the L-Arg pathway in E. coli was successfully overcome by combining gene mutations/deletions. Additionally, the insertion of a gene cluster from Corynebacterium glutamicum enhanced the metabolic flux of the L-Arg pathway. The final strain achieved the highest reported level of L-Arg production by E. coli.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Shilong Hu, Mingyue Fei, Beibei Fu, Mingjing Yu, Panhong Yuan, Biao Tang, Hua Yang, Dongchang Sun
Summary: By protein and metabolic engineering, Escherichia coli Nissle 1917 (EcN) was successfully engineered to produce beta-alanine from glucose. The production of beta-alanine was increased by optimizing enzyme activity, metabolic flux, and culture medium. The engineered EcN obtained a high yield of beta-alanine in fed-batch fermentation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Shunqing Xu, Bin Zhang, Wanhe Chen, Kai Ye, Jian Shen, Pengfu Liu, Jiequn Wu, Hong Wang, Xiaohe Chu
Summary: This article describes the development of a microbial cell factory in Escherichia coli that produces ectoine at high titers, aiming to develop efficient and environmentally friendly bio-based ectoine production technology. The research provides important achievements and potential for industrial applications.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Daisuke Nonaka, Ryosuke Fujiwara, Yuuki Hirata, Tsutomu Tanaka, Akihiko Kondo
Summary: The study demonstrated an enhanced production efficiency of 1,2-propanediol using metabolically engineered Escherichia coli, with successful synthesis from glucose and cellobiose. Improved production was achieved by deleting competing pathways and inactivating the PTS system to reduce carbon flux towards downstream glycolysis.
BIORESOURCE TECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Pradeep Kumar, HyunA Park, Yong Yuk, Hayan Kim, Jihwan Jang, Raviteja Pagolu, SeoA Park, Chanseo Yeo, Kwon-Young Choi
Summary: 1,4-Butanediol (1,4-BDO) is an important industrial chemical that is currently produced through energy-intensive petrochemical processes, leading to issues such as non-renewability, environmental contamination, and high production costs. However, there has been a shift towards sustainable bioproduction of 1,4-BDO using microorganisms and advanced techniques like metabolic engineering and synthetic biology. This article discusses the current status, future prospects, and challenges associated with environmentally friendly and bio-based production strategies for 1,4-BDO.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Microbiology
Mengyang Lei, Zetian Qiu, Leilei Guan, Zheng Xiang, Guang-Rong Zhao
Summary: In this study, a metabolic engineering approach was employed to produce Z,Z-farnesol (Z,Z-FOH) in Escherichia coli. By testing various enzymes and mutants, the optimal strain was able to achieve the highest reported titer of Z,Z-FOH in microbes. This work not only provides insights into the de novo biosynthesis of Z,Z-FOH in E. coli, but also lays a foundation for the development of synthetic E. coli cell factories for other cis-configuration terpenoids.
Editorial Material
Biotechnology & Applied Microbiology
Connor L. Trotter, Gautham S. Babu, Stephen Wallace
Summary: In the past decade, there have been various efforts to replace unsustainable chemical syntheses with green biosynthetic alternatives. In a significant study, Yim et al. used an in silico-to-in vivo workflow to achieve high-level bioproduction of the unnatural small molecule 1,4-butanediol in Escherichia coli.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Electrochemistry
Jiyun Baek, Changman Kim, Young Eun Song, Da Seul Kong, Sakuntala Mutyala, Eun-Hee Seol, Jung Rae Kim
Summary: This paper reports an electrode-assisted non-native pathway regulation using a potentiostat to induce a change in intracellular redox state via bacteria-electrode electron transfer. By controlling the electrode potential, the production of 3-hydroxypropionic acid or 1,3-propanediol in E. coli was increased or enhanced, respectively.
ELECTROCHIMICA ACTA
(2022)
Article
Biotechnology & Applied Microbiology
Carlos Chocarro-Wrona, Juan de Vicente, Cristina Antich, Gema Jimenez, Daniel Martinez-Moreno, Esmeralda Carrillo, Elvira Montanez, Patricia Galvez-Martin, Macarena Peran, Elena Lopez-Ruiz, Juan Antonio Marchal
Summary: The study evaluated a novel material for potential application in cartilage tissue engineering, demonstrating its mechanical properties close to native cartilage and biocompatibility.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2021)
Article
Meteorology & Atmospheric Sciences
Robert Szep, Elena Mateescu, Ion-Andrei Nita, Marius-Victor Birsan, Zsolt Bodor, Agnes Keresztesi
ATMOSPHERIC RESEARCH
(2018)
Article
Environmental Sciences
Robert Szep, Zsolt Bodor, Ildiko Miklossy, Ion-Andrei Nita, Oana A. Oprea, Agnes Keresztesi
SCIENCE OF THE TOTAL ENVIRONMENT
(2019)
Article
Environmental Sciences
Agnes Keresztesi, Marius-Victor Birsan, Ion-Andrei Nita, Zsolt Bodor, Robert Szep
ENVIRONMENTAL SCIENCES EUROPE
(2019)
Article
Environmental Sciences
Agnes Keresztesi, Ion-Andrei Nita, Marius-Victor Birsan, Zsolt Bodor, Robert Szep
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2020)
Article
Meteorology & Atmospheric Sciences
Agnes Keresztesi, Ion-Andrei Nita, Marius-Victor Birsan, Zsolt Bodor, Timea Pernyeszi, Miruna Mihaela Micheu, Robert Szep
ATMOSPHERIC RESEARCH
(2020)
Article
Environmental Sciences
Zsolt Bodor, Katalin Bodor, Agnes Keresztesi, Robert Szep
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2020)
Article
Biotechnology & Applied Microbiology
Zsolt Bodor, Andrea (Iuhasz) Fazakas, Katalin Bodor, Erika Kovacs, Ildiko Miklossy, Beata Albert
ROMANIAN BIOTECHNOLOGICAL LETTERS
(2020)
Article
Environmental Sciences
Agnes Keresztesi, Ion-Andrei Nita, Reka Boga, Marius-Victor Birsan, Zsolt Bodor, Robert Szep
ENVIRONMENTAL RESEARCH
(2020)
Article
Biotechnology & Applied Microbiology
Marta Balazs, Hunor Bartos, Szabolcs Lanyi, Zsolt Bodor, Ildiko Miklossy
Summary: In this study, in silico metabolic modeling was used to characterize Basfia succiniciproducens under different conditions, and it was found that CO2/AIR gas phase had a positive effect on target product formation.
BIOTECHNOLOGY LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Zsolt Bodor, Szabolcs Lanyi, Beata Albert, Katalin Bodor, Aurelia Cristina Nechifor, Ildiko Miklossy
Article
Chemistry, Multidisciplinary
Zsolt Bodor, Lehel Tompos, Aurelia Cristina Nechifor, Katalin Bodor
Article
Geography
Agnes Keresztesi, Reka Boga, Zsolt Bodor, Katalin Bodor, Szende Tonk, Gyorgy Deak, Ion-Andrei Nita
PRESENT ENVIRONMENT AND SUSTAINABLE DEVELOPMENT
(2019)
Article
Geography
Reka Boga, Zsolt Bodor, Katalin Bodor, Szende Tonk, Gyorgy Deak, Timea Pernyeszi, Ion-Andrei Nita
PRESENT ENVIRONMENT AND SUSTAINABLE DEVELOPMENT
(2019)