Article
Biology
Dawafuti Sherpa, Judith Mueller, Oezge Karayel, Peng Xu, Yu Yao, Jakub Chrustowicz, Karthik Gottemukkala, Christine Baumann, Annette Gross, Oliver Czarnecki, Wei Zhang, Jun Gu, Johan Nilvebrant, Sachdev S. Sidhu, Peter J. Murray, Matthias Mann, Mitchell J. Weiss, Brenda A. Schulman, Arno F. Alpi
Summary: This study measured the proteomic landscape of in vitro human erythropoiesis models and found dynamic differential expression of subunits of the CTLH E3 ubiquitin ligase complex, as well as changes in the protein abundance of UBE2H. Inactivation of CTLH E3 assemblies or UBE2H led to defects in erythroid progenitors, including accelerated maturation and inefficient enucleation. These findings suggest that dynamic changes in UBE2H-CTLH E2-E3 modules control the orderly progression of human erythropoiesis.
Article
Genetics & Heredity
Hongbiao Ran, Chunyan Li, Ming Zhang, Jincheng Zhong, Hui Wang
Summary: Ubiquitination, a post-transcriptional modification, plays a crucial role in protein degradation and is involved in various physiological and pathological processes, including animal adipogenesis. The E3 ligases have been found to regulate adipogenesis, but their role in the recognition and connection between substrate and ubiquitin during ubiquitination has been overlooked. This review summarizes the regulatory and modification targets of E3 ligases in animal adipogenesis, explains the regulatory mechanisms in lipogenic pathways, and analyzes existing positive results, providing valuable research directions for further understanding the regulatory mechanisms of E3 ligases in animal adipogenesis.
Review
Pharmacology & Pharmacy
Marcin Cieslak, Marta Slowianek
Summary: The traditional low-molecular-weight drugs target specific biological targets with receptor or enzymatic activity to inhibit their function. However, there are disease proteins that cannot be targeted using this approach. PROTACs, on the other hand, can bind both the protein of interest and the E3 ubiquitin ligase complex, leading to degradation. This review focuses on PROTACs recruiting CRBN E3 ubiquitin ligase and targeting various proteins involved in tumorigenesis.
Article
Chemistry, Medicinal
Dingqiang Fu, Yi Yuan, Fengming Qin, Yan Xu, Xin Cui, Guangxun Li, Shaohua Yao, Yun Deng, Zhuo Tang
Summary: This study designed and synthesized a series of degraders using PROTAC technology that directly targeted human tyrosinase, with the best PROTAC TD9 showing promising results in inducing tyrosinase degradation and highlighting the potential for treating tyrosinase-related disorders.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Jun Goto, Yoichiro Otaki, Tetsu Watanabe, Masafumi Watanabe
Summary: Despite medical advances, cardiac disease remains a significant health issue with high mortality rates. Maladaptive cardiac remodeling, like hypertrophy and fibrosis, is a risk factor for heart failure, underscoring the important role of ubiquitylation in cardiac disease development. HECT-type E3 ligases play a crucial role in ubiquitylation and have been implicated in a wide range of human diseases, including cardiac diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Virology
Jianing Dong, Patrick Paszkowski, Dana Kocincova, Robert J. Ingham
Summary: p28 is a poxvirus-encoded E3 ubiquitin ligase that plays a role in virus production and genome replication, but its function depends on the infection level and cell type. Deletion of the p28 gene leads to compromised virus production and genome replication, which is influenced by the infection level and cell type.
Article
Neurosciences
Na Liu, Miao-Miao Lin, Yan Wang
Summary: Despite the increasing incidence and prevalence of neurodegenerative diseases, effective treatment strategies are lacking. This systematic literature review explores the involvement of E3 ubiquitin ligases and deubiquitinating enzymes in the pathogenesis mechanisms of these diseases, aiming to identify promising therapeutic targets.
MOLECULAR NEUROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Debdeep Dutta, Vartika Sharma, Mousumi Mutsuddi, Ashim Mukherjee
Summary: Notch signaling is an evolutionarily conserved pathway widely used for multiple cellular events during development. The pathway is tightly regulated by physical and biochemical modifications of the Notch receptor and its ligand, as well as through the process of ubiquitination carried out by E3 ubiquitin ligases to maintain proper signaling outcomes. Dysregulation of ubiquitination in Notch signaling can lead to abnormal signaling and contribute to various human diseases.
Article
Virology
Lei Hou, Xiaohan Hu, Jinshuo Guo, Rong Quan, Li Wei, Jing Wang, Jiangwei Song, Jue Liu
Summary: In this study, we found that aMPV/C infection induces MAVS degradation through the ubiquitin-proteasome pathway at the post-translational level. Furthermore, specific amino acid sites were identified as pivotal in the formation of polyubiquitin chains during MAVS degradation. E3 ubiquitin ligases targeting MAVS were also identified, with RNF5 shown to be involved in MAVS degradation in aMPV/C-infected cells.
Article
Immunology
Cinzia Fionda, Helena Stabile, Rosa Molfetta, Andrea Kosta, Giovanna Peruzzi, Silvia Ruggeri, Alessandra Zingoni, Cristina Capuano, Alessandra Soriani, Rossella Paolini, Angela Gismondi, Marco Cippitelli, Angela Santoni
Summary: The protein CRBN plays a critical role in regulating NK cell functions, including cytoskeleton rearrangement, cytotoxicity, and migration. CRBN depletion leads to reduced cytotoxicity, impairment of synapse formation, and decreased migration ability in NK cells. Additionally, CRBN is involved in the activation of Rac1, a key mediator of cytoskeleton dynamics.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
M. Heider, Ruth Eichner, Jacob Stroh, Volker Morath, Anna Kuisl, Jana Zecha, Jannis Lawatscheck, Kheewoong Baek, Anne-Kathrin Garz, Martina Rudelius, Friedrich-Christian Deuschle, Ulrich Keller, Simone Lemeer, Mareike Verbeek, Katharina S. Gotze, Arne Skerra, Wolfgang A. Weber, Johannes Buchner, Brenda A. Schulman, Bernhard Kuster, Vanesa Fernandez-Saiz, Florian Bassermann
Summary: This study identifies the role of the CRBN-AHA1-HSP90 axis in the biogenesis of transmembrane proteins, linking IMiD activity to tumor metabolism, and nominating CD98hc and LAT1 as attractive diagnostic and therapeutic targets in MM.
Article
Microbiology
Xing Yang, Zhijuan Hu, Jingjie Yuan, Run Zou, Yilan Wang, Xuan Peng, Shan Xu, Chengjian Xie
Summary: Two ubiquitin ligase E3 genes (VdBre1 and VdHrd1) in Verticillium dahliae are intrinsically associated with virulence, and their deletion significantly impairs the ability of the fungus to colonize plants and form penetration pegs. VdBre1 governs the lipid metabolism pathway, while VdHrd1 participates in endoplasmic-reticulum-related processes. The Delta VdBre1 mutant shows a significant decrease in histone ubiquitination and histone H3K4 trimethylation levels.
Review
Cell Biology
Kan Lu, Yonglong Pan, Zhao Huang, Huifang Liang, Ze-Yang Ding, Bixiang Zhang
Summary: The TRIM protein family is a versatile and highly conserved group of proteins that function as E3 ligases, regulating the development of hepatocellular carcinoma.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Biology
Lu Zhu, Qing Zhang, Ciro D. Cordeiro, Sudeep Banjade, Richa Sardana, Yuxin Mao, Scott D. Emr
Summary: The study reveals a novel ubiquitination modification implemented by Rsp5 adaptor proteins, which controls the recruitment and activity of Rsp5 to facilitate the turnover of membrane proteins.
Review
Chemistry, Medicinal
Harbinder Singh, Devendra K. Agrawal
Summary: Protease-targeted chimeras (PROTACs) are a novel therapeutic approach that utilizes the ubiquitin-proteasome system for targeted protein degradation. PROTACs based on small-molecule inhibitors have several advantages and cereblon-based PROTACs have promising clinical potential.
FUTURE MEDICINAL CHEMISTRY
(2022)
Article
Neurosciences
Charlotte C. Bavley, Richard C. Rice, Delaney K. Fischer, Amanda K. Fakira, Maureen Byrne, Maria Kosovsky, Bryant K. Rizzo, Dolores Del Prete, Armin Alaedini, Jose A. Moron, Joseph J. Higgins, Luciano D'Adamio, Anjali M. Rajadhyaksha
JOURNAL OF NEUROSCIENCE
(2018)
Article
Multidisciplinary Sciences
Fabrizio Biundo, Dolores Del Prete, Hong Zhang, Ottavio Arancio, Luciano D'Adamio
SCIENTIFIC REPORTS
(2018)
Correction
Psychiatry
F. Biundo, C. d'Abramo, M. D. Tambini, H. Zhang, D. Del Prete, F. Vitale, L. Giliberto, O. Arancio, L. D'Adamio
TRANSLATIONAL PSYCHIATRY
(2018)
Article
Neurosciences
Wen Yao, Marc D. Tambini, Xinran Liu, Luciano D'Adamio
JOURNAL OF NEUROSCIENCE
(2019)
Article
Neurosciences
Erica Acquarone, Elentina K. Argyrousi, Manon van den Berg, Walter Gulisano, Mauro Fa, Agnieszka Staniszewski, Elisa Calcagno, Elisa Zuccarello, Luciano D'Adamio, Shi-Xian Deng, Daniela Puzzo, Ottavio Arancio, Jole Fiorito
MOLECULAR NEURODEGENERATION
(2019)
Article
Cell Biology
Marc D. Tambini, Wen Yao, Luciano D'Adamio
Article
Multidisciplinary Sciences
Marc D. Tambini, Luciano D'Adamio
SCIENTIFIC REPORTS
(2020)
Article
Biology
Marc D. Tambini, Kelly A. Norris, Luciano D'Adamio
Article
Medicine, Research & Experimental
Daniela Puzzo, Elentina K. Argyrousi, Agnieszka Staniszewski, Hong Zhang, Elisa Calcagno, Elisa Zuccarello, Erica Acquarone, Mauro Fa', Domenica D. Li Puma, Claudio Grassi, Luciano D'Adamio, Nicholas M. Kanaan, Paul E. Fraser, Ottavio Arancio
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Article
Biology
Siqiang Ren, Wen Yao, Marc D. Tambini, Tao Yin, Kelly A. Norris, Luciano D'Adamio
Review
Health Care Sciences & Services
Cristina D'Abramo, Luciano D'Adamio, Luca Giliberto
JOURNAL OF PERSONALIZED MEDICINE
(2020)
Article
Biochemistry & Molecular Biology
Tao Yin, Wen Yao, Kelly A. Norris, Luciano D'Adamio
Summary: The study of rats carrying the Danish mutation in the Itm2b gene revealed early pathogenic changes related to neuronal communication and synaptic transmission. These alterations may impair learning and memory processes, resembling the observations in mice carrying other related gene mutations.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Editorial Material
Medicine, Research & Experimental
Luciano D'Adamio
EMBO MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Tao Yin, Wen Yao, Alexander D. Lemenze, Luciano D'Adamio
Summary: Mutations in the ITM2b gene associated with FDD and FBD lead to a reduction in BRI2 levels and function at synapses, resulting in decreased glutamatergic transmission. These mutations also impact the maturation of BRI2, leading to lower levels of functional mature BRI2 protein at synapses.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Wen Yao, Tao Yin, Marc D. Tambini, Luciano D'Adamio
SCIENTIFIC REPORTS
(2019)