Substrate Ubiquitination Controls the Unfolding Ability of the Proteasome

Incomplete proteasomal degradation of green fluorescent proteins in the context of tandem fluorescent protein timers

(2016) Anton Khmelinskii et al.   MOLECULAR BIOLOGY OF THE CELL

Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome

(2016) Y. Shi et al.   SCIENCE

Regulation of Proteasomal Degradation by Modulating Proteasomal Initiation Regions

(2015) Kazunobu Takahashi et al.   ACS Chemical Biology

Top-Down 193-nm Ultraviolet Photodissociation Mass Spectrometry for Simultaneous Determination of Polyubiquitin Chain Length and Topology

(2015) Joe R. Cannon et al.   ANALYTICAL CHEMISTRY

KPC1-Mediated Ubiquitination and Proteasomal Processing of NF-κB1 p105 to p50 Restricts Tumor Growth

(2015) Yelena Kravtsova-Ivantsiv et al.   CELL

Assembly and Specific Recognition of K29- and K33-Linked Polyubiquitin

(2015) Martin A. Michel et al.   MOLECULAR CELL

Deubiquitinase-based analysis of ubiquitin chain architecture using Ubiquitin Chain Restriction (UbiCRest)

(2015) Manuela K Hospenthal et al.   Nature Protocols

Sequence composition of disordered regions fine-tunes protein half-life

(2015) Susan Fishbain et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Ubp6 deubiquitinase controls conformational dynamics and substrate degradation of the 26S proteasome

(2015) Charlene Bashore et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural characterization of the interaction of Ubp6 with the 26S proteasome

(2015) Antje Aufderheide et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nonspecific yet decisive: Ubiquitination can affect the native-state dynamics of the modified protein

(2015) Yulian Gavrilov et al.   PROTEIN SCIENCE

Substrate degradation by the proteasome: A single-molecule kinetic analysis

(2015) Y. Lu et al.   SCIENCE

Sequence composition of disordered regions fine-tunes protein half-life

(2015) Susan Fishbain et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Ubp6 deubiquitinase controls conformational dynamics and substrate degradation of the 26S proteasome

(2015) Charlene Bashore et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Monitoring Keap1–Nrf2 interactions in single live cells

(2014) Liam Baird et al.   BIOTECHNOLOGY ADVANCES

Slipping up: Partial substrate degradation by ATP-dependent proteases

(2014) Nicholas D. Nassif et al.   IUBMB LIFE

Dss1 Is a 26S Proteasome Ubiquitin Receptor

(2014) Konstantinos Paraskevopoulos et al.   MOLECULAR CELL

Structure of the Rpn11–Rpn8 dimer reveals mechanisms of substrate deubiquitination during proteasomal degradation

(2014) Evan J Worden et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Crystal structure of the proteasomal deubiquitylation module Rpn8-Rpn11

(2014) G. R. Pathare et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome

(2014) P. Unverdorben et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The complexity of recognition of ubiquitinated substrates by the 26S proteasome

(2013) Aaron Ciechanover et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Ubiquitin-independent proteasomal degradation

(2013) Jenny Erales et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Ubiquitinated Proteins Activate the Proteasomal ATPases by Binding to Usp14 or Uch37 Homologs

(2013) Andreas Peth et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Reconstitution of the 26S proteasome reveals functional asymmetries in its AAA+ unfoldase

(2013) Robyn Beckwith et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Sequence- and Species-Dependence of Proteasomal Processivity

(2012) Daniel A. Kraut et al.   ACS Chemical Biology

The N-terminal domain of Rpn4 serves as a portable ubiquitin-independent degron and is recognized by specific 19S RP subunits

(2012) Seung-Wook Ha et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

The Ubiquitin-Proteasome System of Saccharomyces cerevisiae

(2012) D. Finley et al.   GENETICS

Complete subunit architecture of the proteasome regulatory particle

(2012) Gabriel C. Lander et al.   NATURE

Tandem fluorescent protein timers for in vivo analysis of protein dynamics

(2012) Anton Khmelinskii et al.   NATURE BIOTECHNOLOGY

Atypical ubiquitylation — the unexplored world of polyubiquitin beyond Lys48 and Lys63 linkages

(2012) Yogesh Kulathu et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Proteasomal Degradation from Internal Sites Favors Partial ProteolysisviaRemote Domain Stabilization

(2011) Daniel A. Kraut et al.   ACS Chemical Biology

ClpX(P) Generates Mechanical Force to Unfold and Translocate Its Protein Substrates

(2011) Rodrigo A. Maillard et al.   CELL

A Three-part Signal Governs Differential Processing of Gli1 and Gli3 Proteins by the Proteasome

(2011) Erin K. Schrader et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Dependence of Proteasome Processing Rate on Substrate Unfolding

(2011) Allen Henderson et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The N-Terminal Unstructured Domain of Yeast ODC Functions as a Transplantable and Replaceable Ubiquitin-Independent Degron

(2011) Daniela Gödderz et al.   JOURNAL OF MOLECULAR BIOLOGY

Stepwise Unfolding of a β Barrel Protein by the AAA+ ClpXP Protease

(2011) Andrew R. Nager et al.   JOURNAL OF MOLECULAR BIOLOGY

High-Yield Expression in Escherichia coli and Purification of Mouse Ubiquitin-Activating Enzyme E1

(2011) Andreia F. Carvalho et al.   MOLECULAR BIOTECHNOLOGY

Defining the geometry of the two-component proteasome degron

(2011) Tomonao Inobe et al.   Nature Chemical Biology

Reversible inhibitor of p97, DBeQ, impairs both ubiquitin-dependent and autophagic protein clearance pathways

(2011) T.-F. Chou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Development of an efficient E. coli expression and purification system for a catalytically active, human Cullin3–RINGBox1 protein complex and elucidation of its quaternary structure with Keap1

(2010) Evan Small et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

The role of the proteasome in heart disease

(2010) Yi-Fan Li et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

Role of ubiquitin–proteasome-mediated proteolysis in nervous system disease

(2010) Ashok N. Hegde et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

Priming and Extending: A UbcH5/Cdc34 E2 Handoff Mechanism for Polyubiquitination on a SCF Substrate

(2010) Kenneth Wu et al.   MOLECULAR CELL

Enhancement of proteasome activity by a small-molecule inhibitor of USP14

(2010) Byung-Hoon Lee et al.   NATURE

Recognition and Processing of Ubiquitin-Protein Conjugates by the Proteasome

(2009) Daniel Finley   Annual Review of Biochemistry

Variably modulated gating of the 26S proteasome by ATP and polyubiquitin

(2009) Xiaohua Li et al.   BIOCHEMICAL JOURNAL

Quantitative Proteomics Reveals the Function of Unconventional Ubiquitin Chains in Proteasomal Degradation

(2009) Ping Xu et al.   CELL

Proteasome Regulators: Activators and Inhibitors

(2009) Li Huang et al.   CURRENT MEDICINAL CHEMISTRY

Lysine 63-linked polyubiquitin chain may serve as a targeting signal for the 26S proteasome

(2009) Yasushi Saeki et al.   EMBO JOURNAL

ATP-dependent Proteases Differ Substantially in Their Ability to Unfold Globular Proteins

(2009) Prakash Koodathingal et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Lysine 48 and Lysine 63 Ubiquitin Conjugates Are Processed Differently by the 26 S Proteasome

(2009) Andrew D. Jacobson et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Polyubiquitination by HECT E3s and the Determinants of Chain Type Specificity

(2009) H. C. Kim et al.   MOLECULAR AND CELLULAR BIOLOGY

Ubiquitinated Proteins Activate the Proteasome by Binding to Usp14/Ubp6, which Causes 20S Gate Opening

(2009) Andreas Peth et al.   MOLECULAR CELL

Targeting the ubiquitin system in cancer therapy

(2009) Daniela Hoeller et al.   NATURE

Polyubiquitin substrates allosterically activate their own degradation by the 26S proteasome

(2009) Dawadschargal Bech-Otschir et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Single-Molecule Studies of RNA Polymerase: Motoring Along

(2008) Kristina M. Herbert et al.   Annual Review of Biochemistry

Protein unfolding by a AAA+ protease is dependent on ATP-hydrolysis rates and substrate energy landscapes

(2008) Andreas Martin et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY