Article
Chemistry, Applied
Lin Wei, Yingying Xu, Min Du, Ying Fan, Ruyi Zou, Xiangyu Xu, Qingdong Zhang, Yu-Zhong Zhang, Wenshuang Wang, Fuchuan Li
Summary: A novel 4-O-endosulfatase (endoBI4SF) was identified, which can efficiently remove 4-O-sulfated groups inside various CS/DS polysaccharides. This provides a much-needed tool to tailor the sulfation patterns and explore the related functions of 4-O-sulfated CS/DS chains.
CARBOHYDRATE POLYMERS
(2023)
Review
Biochemistry & Molecular Biology
Harkanwalpreet Sodhi, Alyssa Panitch
Summary: Glycosaminoglycans play a crucial role in tissue engineering by driving stem cell fate and promoting cell proliferation, and recent studies have explored their use in tissue constructs. The research trends in this area are evaluated, providing insights into future directions.
Review
Cell Biology
Anthony J. Hayes, James Melrose
Summary: Chondroitin sulfate plays crucial roles in cell regulation and structural support in the central and peripheral nervous systems, controlling tissue function and homeostasis, and playing key roles in tissue recovery following neural injuries.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Food Science & Technology
Evellin Balbinot-Alfaro, Claudio R. Novello, Elisangela Dusman, Alexandre T. Alfaro, Helyn P. O. Barddal, Igor Almeida, Veronica E. P. Vicentini, Vilasia G. Martins
Summary: This study found that turkey wattle contains glycosaminoglycans (GAGs) and hyaluronic acid (HA), which have anticoagulant activity, and cytotoxic and antiproliferative activity in tumor cells. The results suggest that turkey wattle can be used as a raw material for extracting bioactive GAGs.
Review
Polymer Science
Qingchi Wang, Lianli Chi
Summary: This review investigates the structural alterations and roles of GAGs/PGs in various diseases, and discusses their potential in disease diagnosis, monitoring, prognosis, and drug development.
Review
Cell Biology
Bin Zhang, Lianli Chi
Summary: Chondroitin sulfate and dermatan sulfate are linear anionic polysaccharides that play important roles in physiological and pathological processes through their interactions with various proteins.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Genetics & Heredity
Shuji Mizumoto, Shuhei Yamada
Summary: Dermatan sulfate (DS) and its proteoglycans play crucial roles in extracellular matrix assembly and cell signaling. Dermatan sulfate epimerase (DSE) and dermatan 4-O-sulfotranserase (D4ST) are rate-limiting factors in DS biosynthesis, and mutations in their corresponding genes can lead to the musculocontractural type of Ehlers-Danlos syndrome.
Article
Chemistry, Physical
Saber Shakibi, Patrick R. R. Onck, Erik van der Giessen
Summary: Glycosaminoglycans (GAGs) are polysaccharides that play crucial roles in biological processes. They are important structural components of cartilage and the extracellular matrix of the brain. Coarse-grained modeling is essential due to the large size of GAGs.
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2023)
Review
Biochemistry & Molecular Biology
Shuji Mizumoto, Shuhei Yamada
Summary: Dermatan sulfate (DS) plays crucial roles in tissue development of the cutis, blood vessels, and bone, and recent studies have revealed its involvement in nerve regeneration, self-renewal and proliferation of neural stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Markel Lafuente-Merchan, Sandra Ruiz-Alonso, Alaitz Zabala, Patricia Galvez-Martin, Juan Antonio Marchal, Blanca Vazquez-Lasa, Idoia Gallego, Laura Saenz-del-Burgo, Jose Luis Pedraz
Summary: This study focuses on the development of nanocellulose-alginate based bioinks for 3D bioprinting for cartilage regeneration. The addition of chondroitin sulfate and dermatan sulfate improves the characteristics of the ink and promotes differentiation to cartilage.
MACROMOLECULAR BIOSCIENCE
(2022)
Review
Cell Biology
Shuji Mizumoto, Shuhei Yamada
Summary: CS/DS and HS are formed through stepwise addition of respective monosaccharides by glycosyltransferases, and their structural diversities are essential for various biological activities. Knockout mice deficient in enzymes responsible for their biosynthesis provide valuable models for studying genetic disorders related to chondroitin and dermatan sulfate.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Microbiology
Lin Wei, Qingdong Zhang, Danrong Lu, Min Du, Xiangyu Xu, Wenshuang Wang, Yu-Zhong Zhang, Xunyi Yuan, Fuchuan Li
Summary: This study identified two novel chondroitin sulfate/dematan sulfate sulfatases that can hydrolyze sulfate groups, providing insights for the structural and functional studies of chondroitin sulfate/dermatan sulfate.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wenshuang Wang, Cedric Przybylski, Xiaojuan Cai, Chrystel Lopin-Bon, Runmiao Jiao, Liran Shi, Kazuyuki Sugahara, Jose L. Neira, Regis Daniel, Fuchuan Li
Summary: This study identified a novel CS/DS 4-O-endosulfatase from a marine bacterium and investigated its catalytic mechanism, revealing substrate specificities, action patterns, and a decrease in enzyme activity with increasing substrate size. The deeper understanding of this enzyme provides a useful tool for exploring the structure-function relationship of CS/DS.
BIOCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Deling Shi, Peng He, Yuefan Song, Shuihong Cheng, Robert J. Linhardt, Jonathan S. Dordick, Lianli Chi, Fuming Zhang
Summary: Monkeypox virus (MPXV) is spreading globally, with an increase in cases through intimate personal contact, particularly in the United States. This study shows that the MPXV A29 protein binds to GAGs, including heparin and chondroitin sulfate/dermatan sulfate, and the negative charges on GAGs are crucial for this interaction. GAG analogs, such as pentosan polysulfate and mucopolysaccharide polysulfate, can effectively inhibit the binding of MPXV A29 to heparin.
Article
Biochemistry & Molecular Biology
Min Du, Lin Wei, Min Yuan, Ruyi Zou, Yingying Xu, Xu Wang, Wenshuang Wang, Fuchuan Li
Summary: Two new chondroitinase ABC I enzymes were identified from the intestinal metagenome of CS-fed mice. They have different enzymatic properties and the N-terminal domain plays a critical role in regulating substrate binding and degradation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Oncology
Sofia Giacosa, Catherine Pillet, Irinka Seraudie, Laurent Guyon, Yann Wallez, Caroline Roelants, Christophe Battail, Bertrand Evrard, Frederic Chalmel, Caroline Barette, Emmanuelle Soleilhac, Marie-Odile Fauvarque, Quentin Franquet, Clement Sarrazin, Nicolas Peilleron, Gaelle Fiard, Jean-Alexandre Long, Jean-Luc Descotes, Claude Cochet, Odile Filhol
Summary: Renal cell carcinoma (RCC) is a significant malignancy globally, with poor outcomes when metastatic. Protein kinases play a crucial role in cancer progression, and targeting CK2 and ATM kinases can induce synthetic lethality in RCC cells. The study highlights the potential of targeting CK2 and ATM kinases in VHL-deficient renal carcinoma cells to trigger ROS-dependent apoptosis and improve patient care in metastatic RCC.
Article
Cardiac & Cardiovascular Systems
Claire Bouvard, Ly Tu, Martina Rossi, Agnes Desroches-Castan, Nihel Berrebeh, Elise Helfer, Caroline Roelants, Hequn Liu, Marie Ouarne, Nicolas Chaumontel, Christine Mallet, Christophe Battail, Andreas Bikfalvi, Marc Humbert, Laurent Savale, Thomas Daubon, Pascale Perret, Emmanuelle Tillet, Christophe Guignabert, Sabine Bailly
Summary: BMP9 and BMP10 play redundant roles in cardiovascular homeostasis and pulmonary arterial hypertension, but have specific roles under chronic hypoxic conditions. BMP9 contributes to chronic hypoxia-induced pulmonary vascular remodeling, while BMP10 plays a role in hypoxia-induced cardiac remodeling.
CARDIOVASCULAR RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Kazuchika Nishitsuji, Midori Ikezaki, Shino Manabe, Kenji Uchimura, Yukishige Ito, Yoshito Ihara
Summary: C-Mannosylation affects the biological functions of proteins, especially in thrombospondin 1 repeats. C-Mannosylated WSPW peptide inhibits TGF-beta-mediated synaptogenesis by suppressing Smad 2 activation. The molecular details of how C-mannosylation affects the biological functions of WxxW motifs require further study for clarification.
GLYCOCONJUGATE JOURNAL
(2022)
Article
Anatomy & Morphology
Yoshiko Takeda-Uchimura, Kazuchika Nishitsuji, Midori Ikezaki, Tomoya O. Akama, Yoshito Ihara, Fabrice Allain, Kenji Uchimura
Summary: KS glycan is crucially attached to proteoglycans in the adult brain. Beta3Gn-T7, encoded by B3gnt7, is identified as a major enzyme for KS synthesis in neuropils and the perineuronal region. This suggests that Beta3Gn-T7 in oligodendrocyte lineage cells may play a significant role in forming neuropils and perineuronal nets in the adult brain.
FRONTIERS IN NEUROANATOMY
(2022)
Article
Biochemistry & Molecular Biology
Kenji Uchimura, Kazuchika Nishitsuji, Li-Ting Chiu, Takashi Ohgita, Hiroyuki Saito, Fabrice Allain, Veeranjaneyulu Gannedi, Chi-Huey Wong, Shang-Cheng Hung
Summary: Misregulation and abnormal processing of amyloidogenic proteins lead to amyloidosis, and the synthesized 6-O-phosphorylated HS/HP oligosaccharides have competitive effects on inhibiting Aβ fibril formation.
Article
Biochemistry & Molecular Biology
Yoshiko Takeda-Uchimura, Midori Ikezaki, Tomoya O. Akama, Kaho Nishioka, Yoshito Ihara, Fabrice Allain, Kazuchika Nishitsuji, Kenji Uchimura
Summary: In this study, it was found that CL40-positive sialylated and sulfated glycans are abundant in the pleural mesothelium and are synthesized complementarily by GlcNAc6ST2 and GlcNAc6ST3 under physiological conditions.
Article
Chemistry, Physical
Mazdak Afshar Bakshloo, Safia Yahiaoui, Fabien Piguet, Manuela Pastoriza-Gallego, Regis Daniel, Jerome Mathe, John J. Kasianowicz, Abdelghani Oukhaled
Summary: This study investigates a method for protein identification using nanopores. The capture rate and dwell time of polypeptide fragments in the pore are found to depend on the ionic strength and magnitude of the applied voltage. The method is capable of accurately identifying proteins, but further research is needed to optimize the analysis.
Review
Immunology
Bilal Hussain, Vivek Kasinath, Gabriel P. Ashton-Rickardt, Thomas Clancy, Kenji Uchimura, George Tsokos, Reza Abdi
Summary: High endothelial venules (HEVs) are specialized blood vessels that support the migration of lymphocytes. They are formed in organs affected by chronic inflammation and cancer. HEVs play a crucial role in cancer treatment and immunotherapy. This review discusses the characteristics of HEVs, the mechanisms of their formation, HEV-dependent immunotherapies, and the potential use of HEV-targeted therapies in the treatment of cancer and immune-mediated diseases.
TRENDS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Parisa Bayat, Charlotte Rambaud, Bernard Priem, Matthieu Bourderioux, Melanie Bilong, Salome Poyer, Manuela Pastoriza-Gallego, Abdelghani Oukhaled, Jerome Mathe, Regis Daniel
Summary: This study demonstrates the label-free detection and analysis of glycosaminoglycan molecules using biological nanopores, reaching a high resolution in deciphering structural information. It is an important step towards the ultimate goal of glycosaminoglycan sequencing.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Mazdak Afshar Bakshloo, Safia Yahiaoui, Matthieu Bourderioux, Regis Daniel, Manuela Pastoriza-Gallego, John J. Kasianowicz, Abdelghani Oukhaled
Summary: Alpha-synuclein plays a crucial role in regulating nerve activity. Mutations in this protein can lead to its aggregation and fibril formation, which is associated with neurodegenerative diseases like Parkinson's. Our study demonstrates that a nanometer-scale pore can effectively distinguish between different forms of alpha-synuclein, including wild-type, mutated, and post-translationally modified variants.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Multidisciplinary Sciences
Naoyuki Iwahashi, Midori Ikezaki, Yoshihiro Komohara, Yukio Fujiwara, Tomoko Noguchi, Kaho Nishioka, Kazuko Sakai, Kazuto Nishio, Mitsuharu Ueda, Yoshito Ihara, Kenji Uchimura, Kazuhiko Ino, Kazuchika Nishitsuji
Summary: Recent studies have found that aggregates of mutant p53 proteins can propagate and disrupt normal p53 function in cancer cells. This study demonstrates the involvement of p53 aggregate transmission in poor prognosis and chemotherapy resistance in cancer.
Article
Multidisciplinary Sciences
Yuki Takechi-Haraya, Takashi Ohgita, Mana Kotani, Hiroki Kono, Chihiro Saito, Hiroko Tamagaki-Asahina, Kazuchika Nishitsuji, Kenji Uchimura, Takeshi Sato, Ryuji Kawano, Kumiko Sakai-Kato, Ken-ichi Izutsu, Hiroyuki Saito
Summary: By studying the structural isomers of A2-17 peptide, we found a correlation between the hydrophobic moment and membrane interaction and direct cell penetration. A2-17 L14R/R15L, with the highest hydrophobic moment, showed the most efficient penetration into cells, forming stable membrane pores.
SCIENTIFIC REPORTS
(2022)
