Review
Biochemistry & Molecular Biology
Shuji Mizumoto, Shuhei Yamada
Summary: Dermatan sulfate (DS) plays crucial roles in tissue development of the cutis, blood vessels, and bone, and recent studies have revealed its involvement in nerve regeneration, self-renewal and proliferation of neural stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Genetics & Heredity
Shuji Mizumoto, Shuhei Yamada
Summary: Dermatan sulfate (DS) and its proteoglycans play crucial roles in extracellular matrix assembly and cell signaling. Dermatan sulfate epimerase (DSE) and dermatan 4-O-sulfotranserase (D4ST) are rate-limiting factors in DS biosynthesis, and mutations in their corresponding genes can lead to the musculocontractural type of Ehlers-Danlos syndrome.
Review
Genetics & Heredity
Takahiro Yoshizawa, Tomoki Kosho
Summary: Musculocontractural Ehlers-Danlos syndrome (mcEDS) is a subtype of EDS caused by mutations in the CHST14 or DSE genes, leading to loss of enzymatic activity and disruption of DS biosynthesis. Symptoms include congenital malformations and connective tissue fragility-related manifestations. Mouse models of mcEDS-CHST14 and mcEDS-DSE exhibit similar phenotypes, making them useful for research on pathophysiology and therapy.
Article
Cell Biology
Liming Li, Heping Zheng, Xuepei Ma, Jie Bai, Shumin Ma, Zhuoyue Li, Chong Qin
Summary: Nerve tissue regeneration faces obstacles due to inhibitory glycosaminoglycan chains in the microenvironment. This study identifies Chst15 as a therapeutic target for spinal cord injury (SCI). Inhibition of Chst15 significantly reduces deposition of inhibitory CSPGs and astrocyte migration, promoting motor functional restoration and nerve tissue regeneration in rats with transected spinal cords. This study highlights Chst15 as a potential target for neuroregenerative therapy.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Mahmudul Hasan, Hamed Khakzad, Lotta Happonen, Anders Sundin, Johan Unge, Uwe Mueller, Johan Malmstrom, Gunilla Westergren-Thorsson, Lars Malmstrom, Ulf Ellervik, Anders Malmstrom, Emil Tykesson
Summary: DS-epi1 catalyzes a key step in the biosynthesis of the glycosaminoglycan dermatan sulfate, and a novel mechanism involving a His/double-Tyr motif has been proposed based on structural and docking studies. The high structural similarity of DS-epi1 to bacterial polysaccharide lyases suggests its importance in various diseases, and its structure provides a starting point for designing active site inhibitors.
Review
Genetics & Heredity
Shuji Mizumoto, Shuhei Yamada
Summary: GAGs and proteoglycans play essential roles in constructing the extracellular matrix, tissue development, and cell signaling. Defects in GAGs and core proteins of proteoglycans may lead to various diseases, including skeletal disorders, heart and kidney defects. Further research is needed on congenital disorders associated with defects in GAG biosynthetic enzymes and core proteins of proteoglycans.
FRONTIERS IN GENETICS
(2021)
Article
Cell Biology
Yuko Nitahara-Kasahara, Guillermo Posadas-Herrera, Shuji Mizumoto, Aki Nakamura-Takahashi, Yukiko U. Inoue, Takayoshi Inoue, Yoshihiro Nomura, Shin'ichi Takeda, Shuhei Yamada, Tomoki Kosho, Takashi Okada
Summary: Deficiency of dermatan sulfate in mcEDS causes pathological distribution and functional abnormalities of decorin in the skeletal muscle, leading to disturbances in muscle myogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Yi-Fan Huang, Shuji Mizumoto, Morihisa Fujita
Summary: The study developed a comprehensive glycosylation mapping tool, GlycoMaple, and analyzed the expression levels of GAG biosynthetic genes in various human tissues and tumor tissues using this tool.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kai Kudo, Takashi Kobayashi, Kosuke Kasai, Hiroyuki Nozaka, Toshiya Nakamura
Summary: Salmon nasal cartilage proteoglycan (PG) was orally administered to mice, and its effects on the small intestine were examined. The PG digest recovered from the intestine showed consistent properties with the actinase-digested PG prior to administration. Chondroitin sulfate, a sugar chain of PG, was found to be resistant to digestion in the mouse small intestine, and chondroitin sulfates showed a suppressive effect on colon cancer cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Review
Cell Biology
Anthony J. Hayes, James Melrose
Summary: Chondroitin sulfate plays crucial roles in cell regulation and structural support in the central and peripheral nervous systems, controlling tissue function and homeostasis, and playing key roles in tissue recovery following neural injuries.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Food Science & Technology
Evellin Balbinot-Alfaro, Claudio R. Novello, Elisangela Dusman, Alexandre T. Alfaro, Helyn P. O. Barddal, Igor Almeida, Veronica E. P. Vicentini, Vilasia G. Martins
Summary: This study found that turkey wattle contains glycosaminoglycans (GAGs) and hyaluronic acid (HA), which have anticoagulant activity, and cytotoxic and antiproliferative activity in tumor cells. The results suggest that turkey wattle can be used as a raw material for extracting bioactive GAGs.
Review
Polymer Science
Qingchi Wang, Lianli Chi
Summary: This review investigates the structural alterations and roles of GAGs/PGs in various diseases, and discusses their potential in disease diagnosis, monitoring, prognosis, and drug development.
Review
Cell Biology
Bin Zhang, Lianli Chi
Summary: Chondroitin sulfate and dermatan sulfate are linear anionic polysaccharides that play important roles in physiological and pathological processes through their interactions with various proteins.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Genetics & Heredity
Ayana Hashimoto, Takuya Hirose, Kohei Hashimoto, Shuji Mizumoto, Yuko Nitahara-Kasahara, Shota Saka, Takahiro Yoshizawa, Takashi Okada, Shuhei Yamada, Tomoki Kosho, Takafumi Watanabe, Shinji Miyata, Yoshihiro Nomura
Summary: Loss-of-function mutations in carbohydrate sulfotransferase 14 (CHST14) lead to musculocontractural Ehlers-Danlos syndrome-CHST14 (mcEDS-CHST14), which is characterized by multiple congenital malformations and progressive connective tissue fragility-related symptoms. In this study, we established in vitro models to replicate the pathological processes of mcEDS-CHST14, aiming to better understand its underlying mechanisms. Our findings revealed impaired fibrillar organization and reduced mechanical strength in mcEDS-CHST14-mimicking collagen gels. Additionally, the addition of decorin from mcEDS-CHST14 patients and Chst14(-/-) mice disrupted collagen fibril assembly in vitro. These in vitro models provide valuable insights into the pathomechanisms of mcEDS-CHST14.
Article
Biochemistry & Molecular Biology
Markel Lafuente-Merchan, Sandra Ruiz-Alonso, Alaitz Zabala, Patricia Galvez-Martin, Juan Antonio Marchal, Blanca Vazquez-Lasa, Idoia Gallego, Laura Saenz-del-Burgo, Jose Luis Pedraz
Summary: This study focuses on the development of nanocellulose-alginate based bioinks for 3D bioprinting for cartilage regeneration. The addition of chondroitin sulfate and dermatan sulfate improves the characteristics of the ink and promotes differentiation to cartilage.
MACROMOLECULAR BIOSCIENCE
(2022)