Article
Immunology
Rasmus K. Jensen, Goran Bajic, Mehmet Sen, Timothy A. Springer, Thomas Vorup-Jensen, Gregers R. Andersen
Summary: CR3, also known as Mac-1 or CD11b/CD18, plays a critical role in phagocytosis of complement-opsonized particles. Studies show a high affinity interaction between iC3b and CR3, relying on minor contacts formed between CR3 and regions outside the iC3b thioester domain. This interaction may explain the more efficient phagocytosis elicited by iC3b compared to C3dg.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Qianchen Liu, Alberto Perez
Summary: Integrins on the cell surface interact with functional motifs in the extracellular matrix (ECM), triggering biological actions like migration, adhesion, or growth. Computational tools have been limited in identifying novel binding motifs for integrin domains. Revisiting traditional and novel computational tools for the I-domain of the alpha 2 beta 1 integrin is important for improving their performance in identifying new binding motifs.
FRONTIERS IN CHEMISTRY
(2023)
Article
Biochemical Research Methods
Sipeng Li, Xinya Li, Siqi Yang, Hao Pi, Zheyi Li, Pengju Yao, Qi Zhang, Qingsong Wang, Pingping Shen, Xizhou Li, Jianguo Ji
Summary: This study revealed the proteomic landscape of TNBC-derived exosomes and discovered that CD151 levels in TNBC-derived exosomes were significantly higher than those in exosomes from healthy subjects. CD151 may be a potential therapeutic target for TNBC.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Multidisciplinary Sciences
Hoa Nguyen, Tianwei Jing, Xu Wang
Summary: Integrin alpha(M)beta(2) (Mac-1, CD11b/CD18, CR3) is an important adhesion receptor expressed on monocytes. It is responsible for mediating various cellular processes such as migration, phagocytosis, degranulation, and cell-cell fusion. Despite the lack of structural studies, the alpha I-M-domain plays a crucial role in ligand interactions of Mac-1. This study successfully identified a stable and soluble mutant, Q163C/Q309C, which can be used for NMR characterization and enhance the understanding of alpha I-M-domain-ligand interactions.
Article
Neurosciences
Gidon Levakov, Olaf Sporns, Galia Avidan
Summary: This study investigates the impact of modularity in the face recognition network on human face recognition abilities, finding a positive correlation between modularity and recognition abilities. The correlation is particularly strong within hemispheres, especially within the right hemisphere at an individual level. This study provides the first evidence of the importance of modularity-behavior relationships in the domain of face processing and visual perception.
Article
Chemistry, Analytical
Swati Negi, Parveen Gahlyan, Rashim Bawa, Bholey Singh, Mamta Bhandari, Rita Kakkar, Balaram Pani, Rakesh Kumar
Summary: A new rhodamine based chemosensor S1 was synthesized for selective recognition of Cr3+. The interaction between S1 and different metal ions was studied using fluorescence and UV-visible spectroscopy. The results showed that S1 exhibited significant fluorescence and colorimetric changes for Cr3+ compared to other metal ions. The chemosensor S1 formed a 1 : 1 complex with Cr3+ as confirmed by Job's plot and Benesi-Hildebrand (B-H) plot. Density functional theory (DFT) studies confirmed the coordination of Cr3+ ion to three atoms of S1. The chemosensor S1 had a limit of detection (LOD) of 0.21 μM for Cr3+. Additionally, S1 showed reversibility and colorimetric variation when ethylenediaminetetraacetic acid (EDTA) was added to the S1-Cr3+ solution, mimicking the INHIBIT molecular logic gate. The practical utility of S1 was demonstrated for the detection of Cr3+ in the solid state.
ANALYTICAL METHODS
(2023)
Article
Multidisciplinary Sciences
Ala M. Shaqra, Sarah N. Zvornicanin, Qiu Yu J. Huang, Gordon J. Lockbaum, Mark Knapp, Laura Tandeske, David T. Bakan, Julia Flynn, Daniel N. A. Bolon, Stephanie Moquin, Dustin Dovala, Nese Kurt Yilmaz, Celia A. Schiffer
Summary: The study determined the crystal structures of SARS-CoV-2 main protease (Mpro) bound to substrate peptides, defining the substrate envelope and identifying sites susceptible to drug resistance. These findings provide insights for developing robust inhibitors against SARS-CoV-2 and preventing drug resistance.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Henriette Rubsam, Christina Kronauer, Nikolaj B. Abel, Hongtao Ji, Damiano Lironi, Simon B. Hansen, Marcin Nadzieja, Marie Kolte, Dorte Abel, Noor de Jong, Lene H. Madsen, Huijun Liu, Jens Stougaard, Simona Radutoiu, Kasper R. Andersen
Summary: This study developed a synthetic approach using nanobodies to understand and activate multicomponent receptor complexes, successfully manipulating receptors involved in plant symbiosis with nitrogen-fixing bacteria. The authors found that the Lotus japonicus Nod factor receptors NFR1 and NFR5 form the core receptor complex for initiating root nodule organogenesis and controlling infection. They also discovered that organogenesis signaling is mediated by intracellular kinase domains, while infection requires functional ectodomains. Additionally, evolutionarily distant barley receptors were identified, which could potentially enable nitrogen-fixation engineering in cereals.
Article
Fisheries
Xiaoqing Xiong, Hong Yang, Chunhua Ding, Beibei Qin, Yadong Deng, Liming Xiong, Xiaoyan Liu, Yaoguo Li, Tiaoyi Xiao, Zhao Lv
Summary: In this study, a newly discovered integrin αvβ3 in grass carp was found to interact with viral proteins and mediate antiviral immune response. Furthermore, the expression of this integrin was significantly upregulated in various tissues of grass carp after infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lan Xie, Shanshan Feng, Xiaoling Zhang, Wenlong Zhao, Juan Feng, Chengmei Ma, Ruijun Wang, Weifang Song, Jing Cheng
Summary: This study analyzed the differences in typical alkaloids of Rhizoma Coptidis at a systematic level, providing comprehensive information on their pharmaceutical mechanisms. Berberine and coptisine showed stronger inhibitory effects on cell proliferation than palmatine, with some similarities in cellular pathways. Additionally, these alkaloids had different regulatory effects on genes involved in lipid metabolism.
Article
Multidisciplinary Sciences
Joshua H. Siegle, Xiaoxuan Jia, Severine Durand, Sam Gale, Corbett Bennett, Nile Graddis, Greggory Heller, Tamina K. Ramirez, Hannah Choi, Jennifer A. Luviano, Peter A. Groblewski, Ruweida Ahmed, Anton Arkhipov, Amy Bernard, Yazan N. Billeh, Dillan Brown, Michael A. Buice, Nicolas Cain, Shiella Caldejon, Linzy Casal, Andrew Cho, Maggie Chvilicek, Timothy C. Cox, Kael Dai, Daniel J. Denman, Saskia E. J. de Vries, Roald Dietzman, Luke Esposito, Colin Farrell, David Feng, John Galbraith, Marina Garrett, Emily C. Gelfand, Nicole Hancock, Julie A. Harris, Robert Howard, Brian Hu, Ross Hytnen, Ramakrishnan Iyer, Erika Jessett, Katelyn Johnson, India Kato, Justin Kiggins, Sophie Lambert, Jerome Lecoq, Peter Ledochowitsch, Jung Hoon Lee, Arielle Leon, Yang Li, Elizabeth Liang, Fuhui Long, Kyla Mace, Jose Melchior, Daniel Millman, Tyler Mollenkopf, Chelsea Nayan, Lydia Ng, Kiet Ngo, Thuyahn Nguyen, Philip R. Nicovich, Kat North, Gabriel Koch Ocker, Doug Ollerenshaw, Michael Oliver, Marius Pachitariu, Jed Perkins, Melissa Reding, David Reid, Miranda Robertson, Kara Ronellenfitch, Sam Seid, Cliff Slaughterbeck, Michelle Stoecklin, David Sullivan, Ben Sutton, Jackie Swapp, Carol Thompson, Kristen Turner, Wayne Wakeman, Jennifer D. Whitesell, Derric Williams, Ali Williford, Rob Young, Hongkui Zeng, Sarah Naylor, John W. Phillips, R. Clay Reid, Stefan Mihalas, Shawn R. Olsen, Christof Koch
Summary: The study explores the hierarchical organization of functional connectivity in the mammalian visual system, revealing correlations between neural activity and behavioral choice that increase along the hierarchy. This provides a foundation for understanding coding and signal propagation across hierarchically organized cortical and thalamic visual areas.
Article
Biology
Hirdesh Kumar, Emily E. Williford, Kevin S. Blake, Brett Virgin-Downey, Gautam Dantas, Timothy A. Wencewicz, Niraj H. Tolia
Summary: This study reveals that anhydrotetracycline binds and inhibits type 1 tetracycline destructases (TDases) in a distinct manner compared to type 2 TDases. Inactivation of tetracycline antibiotics by TDases is a significant threat in clinical and agricultural settings. Anhydrotetracycline serves as a pan-TDase inhibitor that targets both type 1 and type 2 TDases. The findings provide valuable insights into the development of future therapies against enzymatic tetracycline resistance.
COMMUNICATIONS BIOLOGY
(2023)
Article
Mathematics
Antonio Lei, Luochen Zhao
Summary: Under certain hypotheses, we improve upon Kobayahsi-Ota's result and show that the same inclusion holds integrally for the Iwasawa main conjecture of f. Our result implies the vanishing of the Iwasawa mu-invariants of several anticyclotomic Selmer groups.
MANUSCRIPTA MATHEMATICA
(2023)
Article
Geochemistry & Geophysics
M. Laporte, L. Bollinger, H. Lyon-Caen, R. Hoste-Colomer, C. Duverger, J. Letort, M. Riesner, B. P. Koirala, M. Bhattarai, T. Kandel, C. Timsina, L. B. Adhikari
Summary: The study focuses on seismic tectonics in the Himalayan range, revealing the close relationship between mid-crustal seismic activity and the geometry of the Main Himalayan Thrust system. The seismic activity is influenced by deep fault behavior, fluids, and may control the rupture extension of different earthquakes, providing insights into the distribution and nature of seismic activity.
GEOPHYSICAL JOURNAL INTERNATIONAL
(2021)
Article
Chemistry, Applied
Xingxing Zhang, Meihua Yu, Xiaoling Zhu, Rui Liu, Qun Lu
Summary: This study investigated the anti-tyrosinase activity, chemical composition, and metabolites of bee pollen extract. It revealed that phenolamides are the main contributors to the anti-tyrosinase activity of bee pollen.
Article
Immunology
Margalida Mateu-Borras, Alex Gonzalez-Alsina, Antonio Domenech-Sanchez, Javier Querol-Garcia, Francisco J. Fernandez, Ma Cristina Vega, Sebastian Alberti
Summary: This study investigates how isolates from CF patients modulate the levels of C5a and identifies the bacterial factors involved. The results show that most isolates from airway chronic infections induce the production and accumulation of C5a, which is attributed to the loss of C5a cleavage by the exoproteases AprA and LasB. Furthermore, it is found that lack of bacterial protease-dependent C5a degradation is due to mutations in the master regulator LasR. These findings suggest that non-cleaving C5a phenotype acquired by LasR variants may contribute to the strong neutrophilia and neutrophil dysfunction in CF patients.
Article
Chemistry, Multidisciplinary
Soumaya Khlifi, Jerome Marrot, Mohamed Haouas, William E. Shepard, Clement Falaise, Emmanuel Cadot
Summary: Researchers have developed a unique supramolecular framework by utilizing the affinity between POM and natural cyclodextrins, allowing for the encapsulation of various substances in large cavities and functionalization; X-ray analysis revealed that the framework forms honeycomb and checkerboard-like networks in α-CD and γ-CD, respectively, while leading to a distorted checkerboard-like structure in β-CD.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Immunology
Inmaculada Serrano, Ana Luque, Francesca Mitjavila, Anna M. Blom, Santiago Rodriguez de Cordoba, M. Cristina Vega, Joan Torras, Josep M. Aran
Summary: C4b-binding protein (C4BP) is a protein that regulates the complement system and has immunomodulatory activity. In this study, a specific isoform and oligomeric form of C4BP were found to have immunomodulatory activity, capable of changing the inflammatory state of dendritic cells, and may represent a new approach for treating autoimmune diseases.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Inmaculada Serrano, Ana Luque, Alexandra Ruiz-Cerulla, Sergio Navas, Anna M. Blom, Santiago Rodriguez de Cordoba, Francisco J. Fernandez, M. Cristina Vega, Francisco Rodriguez-Moranta, Jordi Guardiola, Josep M. Aran
Summary: C4BP(beta-) and PRP6-HO7 showed strong anti-inflammatory and immunomodulatory effects, attenuating histopathological features and preserving intestinal epithelial integrity in murine colitis. These molecules downregulated inflammatory transcripts and reduced circulating inflammatory cytokines and chemokines. They also decreased inflammatory markers in myeloid cells from ulcerative colitis and Crohn's disease patients.
PHARMACOLOGICAL RESEARCH
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Francisco Jose Fernandez, Javier Querol-Garcia, Ana V. Marin, Anais Jimenez, Israel Mares-Mejias, Marta Subias, Mercedes Dominguez, Santiago Rodriguez de Cordoba, Jose Ramon Regueiro, M. Cristina Vega
MOLECULAR IMMUNOLOGY
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Francisco Jose Fernandez, Jorge Santos-Lopez, Ruben Martinez-Barricarte, Javier Querol-Garcia, Hector Martin-Merinero, Sergio Navas-Yuste, Martin Savko, William E. Shepard, Santiago Rodriguez de Cordoba, M. Cristina Vega
MOLECULAR IMMUNOLOGY
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Sergio Navas-Yuste, Karla de la Paz-Garcia, Javier Querol-Garcia, Francisco Jose Fernandez, M. Cristina Vega
MOLECULAR IMMUNOLOGY
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Lucia Alfonso-Gonzalez, Francisco Jose Fernandez, M. Cristina Vega
MOLECULAR IMMUNOLOGY
(2022)