Editorial Material
Cell Biology
Hui Han, Siyi Zheng, Shuibin Lin
Summary: METTL1 and WDR4 act as negative regulators of autophagy in esophageal squamous cell carcinoma (ESCC) by modulating the MTORC1-mediated autophagy signaling pathway, leading to cell death and poor prognosis in ESCC.
Article
Biotechnology & Applied Microbiology
Shenghua Zhu, Yifan Wu, Xinyue Zhang, Sui Peng, Han Xiao, Shuling Chen, Lixia Xu, Tianhong Su, Ming Kuang
Summary: Radiofrequency heat ablation is an effective treatment for hepatocellular carcinoma, but inadequate ablation can result in high recurrence rates. This study reveals that after ablation, the levels of m7G tRNA modification and its methyltransferase complex components METTL1/WDR4 are upregulated in various systems. Functionally, METTL1-mediated m7G tRNA modification promotes HCC metastasis under sublethal heat exposure. Mechanistically, METTL1 and m7G tRNA modification enhance the translation of SLUG/SNAIL in a codon frequency-dependent manner under sublethal heat stress. These findings provide insights into the role of m7G tRNA modification in heat stress responses and HCC recurrence after ablation, with potential implications for targeted therapies.
Article
Biochemistry & Molecular Biology
Zihao Dai, Haining Liu, Junbin Liao, Cheng Huang, Xiaoxue Ren, Wanjie Zhu, Shenghua Zhu, Baogang Peng, Shaoqiang Li, Jiaming Lai, Lijian Liang, Lixia Xu, Sui Peng, Shuibin Lin, Ming Kuang
Summary: Intrahepatic cholangiocarcinoma (ICC) shows upregulation of N-7-methylguanosine (m(7)G) tRNA modification and its methyltransferase complex components, METTL1 and WDR4, which are associated with poor prognosis. METTL/WDR4 play a critical role in promoting ICC cell survival and progression by selectively regulating the translation of oncogenic transcripts through m(7)G-tRNA-decoded codon-frequency-dependent mechanisms. Studies with overexpression and knockout mouse models demonstrate the crucial oncogenic function of Mettl1-mediated m(7)G tRNA modification in promoting ICC tumorigenesis and progression in vivo.
Article
Gastroenterology & Hepatology
Haining Liu, Xuezhen Zeng, Xuxin Ren, Yifan Zhang, Manling Huang, Li Tan, Zihao Dai, Jiaming Lai, Wenxuan Xie, Zebin Chen, Sui Peng, Lixia Xu, Shuling Chen, Shunli Shen, Ming Kuang, Shuibin Lin
Summary: This study reveals that PMN-MDSCs, enriched in advanced ICCs, are significantly correlated with N-7-methylguanosine tRNA methyltransferase METTL1. METTL1 promotes PMN-MDSC accumulation and ICC progression by regulating translational targets like CXCL8 and Cxcl5. Furthermore, co-blockade of METTL1 and its downstream chemokine pathway enhances the efficacy of anti-PD-1 therapy.
Article
Oncology
Jie Chen, Kang Li, Jianwen Chen, Xiaochen Wang, Rongsong Ling, Maosheng Cheng, Zhi Chen, Fangfang Chen, Qianting He, Shuai Li, Caihua Zhang, Yizhou Jiang, Qianming Chen, Anxun Wang, Demeng Chen
Summary: The study revealed that tRNA m(7)G methyltransferase METTL1 promotes the development and malignancy of head and neck squamous cell carcinoma (HNSCC) by regulating global mRNA translation, including the PI3K/AKT/mTOR signaling pathway, and alters the immune landscape.
CANCER COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Yi Yang, Yifu Zhu, Shuai Zhou, Peipei Tang, Ran Xu, Yuwei Zhang, Dongping Wei, Jian Wen, Rick F. Thorne, Xu Dong Zhang, Jun-Lin Guan, Lianxin Liu, Mian Wu, Song Chen
Summary: Autophagy is a fundamental mechanism for cell survival and tissue homeostasis, and TRIM27 has been identified as a negative regulator of the FIP200-ATG13-ULK1 complex. TRIM27 controls the amplitude and duration of autophagy by ubiquitinating ULK1, and the ubiquitination of STK38L kinase plays a key role in ULK1 activation and phosphorylation.
Article
Biochemistry & Molecular Biology
Binbin Chen, Wei Jiang, Ying Huang, Jian Zhang, Peng Yu, Lirong Wu, Hao Peng
Summary: This study reveals that the N-7-methylguanosine tRNA modification enzyme METTL1 and its partner WDR4 are significantly increased in nasopharyngeal carcinoma (NPC) and are associated with poor prognosis. METTL1/WDR4 promotes NPC growth and metastasis through the WNT/β-catenin signaling pathway, as well as inducing epithelial-mesenchymal transition and chemoresistance.
Article
Biology
Zhaoxu Yao, Haibin Ma, Lin Liu, Qian Zhao, Longchao Qin, Xueyan Ren, Chuanjun Wu, Kaili Sun
Summary: This study identified N7-methylguanosine modification-related long non-coding RNAs (m7G-lncRNAs) and built a prognosis prediction model for laryngeal squamous cell carcinoma (LSCC) patients. The model's accuracy was evaluated and a nomogram and receiver operating characteristic (ROC) curves were used to assess its predictive capability.
Article
Oncology
Jialiang Zhou, Jia Wu, Gang Wu, Jianfeng Huang, Yunxia Zhang, Jun Che, Koujun Zhu, Jiqun Geng, Qiang Fan
Summary: This study found that overexpression of TBX18 gene is related to poor radiotherapy efficacy in esophageal squamous cell carcinoma (ESCC). Through a series of cell experiments and animal model experiments, it was discovered that knockdown of TBX18 can increase the sensitivity of ESCC cells to radiotherapy.
RADIOTHERAPY AND ONCOLOGY
(2023)
Article
Oncology
Wenjian Yao, Jianjun Wang, Li Zhu, Xiangbo Jia, Lei Xu, Xia Tian, Shuai Hu, Sen Wu, Li Wei
Summary: This study systematically identified the role of KDM4D/SYVN1/HMGB1 axis in the progression of esophageal squamous cell carcinoma, providing novel biomarkers and potential therapeutic targets.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Yongqing Heng, Yupei Liang, Junqian Zhang, Lihui Li, Wenjuan Zhang, Yanyu Jiang, Shiwen Wang, Lijun Jia
Summary: The neddylation pathway is overactivated in esophageal cancer, and the anticancer agent, Camptothecin (CPT), induces apoptosis and autophagy in cancer cells. CPT inhibits neddylation activity, induces ROS generation, and modulates the NF-kappa B, AMPK/mTOR/ULK1 pathways to promote protective autophagy in esophageal cancer cells. This elucidates a novel mechanism for combinational anti-ESCC therapy with CPT and inhibition of the AMPK/ULK1 pathway.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Chao Song, Hanping Qi, Yongsheng Liu, Yunping Chen, Pilong Shi, Shu Zhang, Jing Ren, Lixin Wang, Yonggang Cao, Hongli Sun
Summary: The study identified a novel regulatory axis involving lncRNA Gm15834/miR-30b-3p/ULK1/autophagy in cardiac hypertrophy, providing a potential therapeutic target for the condition.
Article
Cell Biology
Li Zhu, Jiahe Wang, Zuping Wu, Sirui Chen, Yuying He, Yukun Jiang, Guowen Luo, Zhuoxuan Wu, Yuyu Li, Jing Xie, Shujuan Zou, Chenchen Zhou
Summary: This study investigated the role of AFF4 in the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and identified its mechanism of action. The study found that AFF4 expression was lower in inflamed periodontal tissues and lipopolysaccharides-treated hPDLSCs compared to controls, and it was up-regulated during osteogenic differentiation of hPDLSCs. Further experiments showed that AFF4 regulated the osteogenic differentiation of hPDLSCs by targeting autophagic activity.
CELL PROLIFERATION
(2023)
Article
Oncology
Lei Li, Yuhao Liu, Yahui Zhao, Riyue Feng, Yang Li, Xiao Yu, Zhihua Liu, Luhua Wang
Summary: In this study, USP8 was identified as a novel deubiquitinase for ID1, interacting with and stabilizing ID1 by reducing its ubiquitination. Overexpression of USP8 promotes ESCC tumorigenesis by suppressing ID1 degradation and activating the ID1-TXNIP pathway, whereas knockdown of USP8 leads to an opposite phenotype. The increased expression of USP8 and ID1 correlates with reduced TXNIP expression in ESCC tissues and predicts an advanced tumor stage.
Article
Biochemistry & Molecular Biology
Chaoqun Ma, Qiang Xu, Songqun Huang, Jingwen Song, Minglei Sun, Jingyu Zhang, Guojun Chu, Bili Zhang, Yuan Bai, Xianxian Zhao, Zhongkai Wang, Pan Li
Summary: This study found that miR-26a-5p is downregulated in the plasma of PAH patients, as well as in lung tissues and hypoxia-induced pulmonary arterial smooth muscle cells. HIF-1a specifically interacts with the promoter of miR-26a-5p and inhibits its expression in PASMCs. miR-26a-5p inhibits PASMC autophagy and proliferation by targeting PFKFB3, ULK1, and ULK2. Furthermore, overexpression of miR-26a-5p alleviates pulmonary vascular remodeling and right ventricular hypertrophy in hypoxia-induced PAH rat models.
Article
Cell Biology
Ganping Wang, Yarong Dai, Kang Li, Maosheng Cheng, Gan Xiong, Xiaochen Wang, Shuang Chen, Zhi Chen, Jianwen Chen, Xiuyun Xu, Rong-song Ling, Liang Peng, Demeng Chen
Summary: The study demonstrates that Mettl3-mediated m(6)A modification is crucial for the activation of TEK-VEGF-A-mediated tumor progression and angiogenesis in bladder cancer. Experimental results using transgenic mouse models and bladder cancer stem cell populations showed that depletion of Mettl3 inhibits tumor oncogenesis and progression in bladder cancer.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Yu Liang, Hui Han, Qiuchan Xiong, Chunlong Yang, Lu Wang, Jieyi Ma, Shuibin Lin, Yi-Zhou Jiang
Summary: Knockout of METTL3 in muscle stem cells inhibits cell proliferation and blocks muscle regeneration, while knockin of METTL3 promotes cell proliferation and regeneration. The METTL3-m(6)A-YTHDF1 axis regulates Notch signaling pathway mRNA translation, revealing the important physiological role of METTL3-mediated m(6)A modification in muscle stem cells and regeneration.
STEM CELLS INTERNATIONAL
(2021)
Editorial Material
Biotechnology & Applied Microbiology
Chunlong Yang, Hui Han, Shuibin Lin
Editorial Material
Cell Biology
Hui Han, Siyi Zheng, Shuibin Lin
Summary: METTL1 and WDR4 act as negative regulators of autophagy in esophageal squamous cell carcinoma (ESCC) by modulating the MTORC1-mediated autophagy signaling pathway, leading to cell death and poor prognosis in ESCC.
Review
Oncology
Siyi Zheng, Hui Han, Shuibin Lin
Summary: Growing evidence suggests that the progression of cancer is closely linked to the tumor microenvironment and evasion of the immune system. Recent studies have highlighted the important roles of epigenetic regulators, particularly N-6-methyladenosine (m(6)A) modification, in shaping the tumor microenvironment and restoring immune recognition. These modifications have significant effects on various biological processes, including tumorigenesis and progression. Additionally, abnormal m(6)A modification has been found to play critical functions in regulating tumor immunity.
CANCER BIOLOGY & MEDICINE
(2022)
Article
Oncology
Ying Huang, Jieyi Ma, Cuiyun Yang, Paijia Wei, Minghui Yang, Hui Han, Hua Dong Chen, Tianfang Yue, Shu Xiao, Xuanyu Chen, Zuoqing Li, Yanlai Tang, Jiesi Luo, Shuibin Lin, Libin Huang
Summary: This study identified the critical role and mechanism of METTL1-mediated tRNA m(7)G modification in regulating NBL progression, providing new insights for developing therapeutic approaches for NBL patients. The up-regulation of METTL1 in advanced NBL was found to be an independent risk factor predicting poor prognosis. METTL1 played a crucial role in promoting NBL progression, with downregulation inhibiting puromycin intake efficiency and impacting gene translation efficiency in oncogenic pathways.
BIOMARKER RESEARCH
(2022)
Article
Cell Biology
Chunlong Yang, Xiaoning Cheng, Shenglan Gao, Qingjun Pan
Summary: This study focuses on the elusive role of endothelial cells in the tumor microenvironment (TME) of head and neck squamous cell carcinoma (HNSCC). By using advanced single-cell RNA sequencing technology, the authors identify different subtypes of endothelial cells and construct a prognostic risk model for HNSCC. The findings provide insights into the heterogeneity of the TME at the single-cell level and offer a prognostic model for HNSCC patients.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Endocrinology & Metabolism
Hao Peng, Binbin Chen, Wei Wei, Siyao Guo, Hui Han, Chunlong Yang, Jieyi Ma, Lu Wang, Sui Peng, Ming Kuang, Shuibin Lin
Summary: This study reveals the regulatory role of 18S rRNA modifications in cancer progression and prognosis, particularly in hepatocellular carcinoma. The findings demonstrate the critical functions of METTL5 in promoting HCC tumorigenesis through impaired ribosome assembly and decreased translation of fatty acid metabolism genes. The study also highlights the potential therapeutic strategies targeting METTL5 and ACSL4 for HCC treatment.
Article
Biochemistry & Molecular Biology
Lu Wang, Yu Liang, Rongzhi Lin, Qiuchan Xiong, Peng Yu, Jieyi Ma, Maosheng Cheng, Hui Han, Xiaochen Wang, Ganping Wang, Fengyin Liang, Zhong Pei, Demeng Chen, Quan Yuan, Yi-Zhou Jiang, Shuibin Lin
Summary: METTL5 protein regulates brain function and intelligence by controlling rRNA modification, and Mettl5 knockout results in intellectual disability and abnormal nervous development.
