Article
Biochemistry & Molecular Biology
Ahyeon Jeong, Yena Cho, Minkyeong Cho, Gyu-Un Bae, Dae-Geun Song, Su-Nam Kim, Yong Kee Kim
Summary: PRMT7 is a critical regulator of cell growth and DNA damage response, and its inhibition can cause cell cycle arrest and disruption of DNA repair pathways. Combination with doxorubicin enhances its cytotoxic effect on cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Anyi Liu, Chengxin Yu, Cheng Qiu, Qi Wu, Changsheng Huang, Xun Li, Xiaowei She, Kairui Wan, Lang Liu, Mao Li, Zhihong Wang, Yaqi Chen, Fuqing Hu, Da Song, Kangdi Li, Chongchong Zhao, Haiteng Deng, Xuling Sun, Feng Xu, Senyan Lai, Xuelai Luo, Junbo Hu, Guihua Wang
Summary: Perturbations in TGF-beta signaling can be caused by mutations and posttranslational modifications of SMAD4, including R361 methylation mediated by PRMT5. This study demonstrates that the interaction between PRMT5 and SMAD4, as well as SMAD4 R361 methylation, play critical roles in TGF-beta signaling activation and metastasis. Blocking PRMT5-SMAD4 signaling could be a potential therapeutic strategy for SMAD4 wild-type CRC.
Article
Multidisciplinary Sciences
Ting Shen, Ting Ni, Jiaxuan Chen, Haitao Chen, Xiaopin Ma, Guangwen Cao, Tianzhi Wu, Haisheng Xie, Bin Zhou, Gang Wei, Hexige Saiyin, Suqin Shen, Peng Yu, Qianyi Xiao, Hui Liu, Yuzheng Gao, Xidai Long, Jianhua Yin, Yanfang Guo, Jiaxue Wu, Gong-Hong Wei, Jinlin Hou, De-Ke Jiang
Summary: Most cancer-causing variations are found in gene regulatory elements, such as enhancers. However, enhancer variants predisposing to hepatocellular carcinoma (HCC) have not been reported before. In this study, a genome-wide survey was conducted in 11,958 individuals to identify HCC-susceptible enhancer variants, and rs73613962 within the intronic region of PRMT7 was discovered as a susceptibility locus for HCC. The functional activity of this region as an enhancer was confirmed through experimental assays. The upregulation of PRMT7, which is regulated by this enhancer region through the binding of the transcription factor HNF4A, contributes to HCC-related phenotypes both in vitro and in vivo. This finding sheds light on the pathogenesis of HCC and may provide new opportunities for its prevention and treatment.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Christina H. Acosta, Garrett A. Clemons, Cristiane T. Citadin, William C. Carr, Mariana Sayuri Berto Udo, Vesna Tesic, Henry W. Sanicola, Anne H. Freelin, Jamie B. Toms, J. Dedrick Jordan, Bharat Guthikonda, Krista M. Rodgers, Celeste Yin-Chieh Wu, Reggie Hui -Chao Lee, Hung Wen Lin
Summary: Mild traumatic brain injury (TBI) is common and can result in persistent pathophysiological and functional deficits. In this study, a three-hit paradigm of repetitive and mild TBI (rmTBI) was used to investigate the effects on neurovascular coupling, blood-brain barrier (BBB) permeability, mitochondrial respiration, and PRMT7 activity. The findings suggest that rmTBI leads to neurovascular uncoupling, BBB leakage, altered mitochondrial dynamics, and decreased PRMT7 levels and activity. However, increasing PRMT7 levels in vivo through overexpression restores neurovascular coupling, prevents BBB leakage, and promotes mitochondrial respiration, implying a protective and functional role of PRMT7 in rmTBI.
EXPERIMENTAL NEUROLOGY
(2023)
Article
Cell Biology
Chang Liu, Waiyi Zou, Danian Nie, Shuyi Li, Chen Duan, Min Zhou, Peilong Lai, Shengyong Yang, Sen Ji, Yangqiu Li, Mei Mei, Shilai Bao, Yanli Jin, Jingxuan Pan
Summary: This study investigates the role of PRMT family member PRMT7 in the maintenance of leukemia stem cells (LSCs) in chronic myeloid leukemia (CML). The research shows that targeting PRMT7 delays leukemia development and impairs self-renewal of LSCs without affecting normal hematopoiesis. Mechanistically, loss of PRMT7 leads to reprogramming of glycine metabolism, generating methylglyoxal which is detrimental to LSCs. These findings suggest PRMT7 as a potential therapeutic target for eradicating LSCs in CML.
Article
Cell Biology
Nivine Srour, Oscar D. Villarreal, Swanand Hardikar, Zhenbao Yu, Samuel Preston, Wilson H. Miller, Magdelena M. Szewczyk, Dalia Barsyte-Lovejoy, Han Xu, Taiping Chen, Sonia Del Rincon, Stephane Richard
Summary: Combining PRMT7 inhibition with ICI therapy induces a strong anti-tumor T cell immunity and restrains tumor growth in vivo by increasing immune cell infiltration.
Article
Cell Biology
Yachun Jia, Xiao Yu, Rui Liu, Luyi Shi, Hua Jin, Dan Yang, Xiaofeng Zhang, Ying Shen, Yuandong Feng, Peihua Zhang, Yi Yang, Linlin Zhang, Pengyu Zhang, Zongfang Li, Aili He, Guangyao Kong
Summary: Epigenetic modifications, particularly the expression of protein arginine methyltransferase 1 (PRMT1), play a crucial role in the pathogenesis of multiple myeloma (MM). High expression of PRMT1 in MM patients is positively correlated with MM stages and adverse prognosis. Silencing PRMT1 inhibits MM cell proliferation and tumorigenesis through downregulation of oxidative phosphorylation (OXPHOS) and the methylation of NDUFS6. Furthermore, the combination of PRMT1 inhibitor and bortezomib shows synergy in inhibiting MM progression.
CELL DEATH & DISEASE
(2023)
Article
Microbiology
Yishan Lu, Guozhen An, Xue Wang, Yunlin Tang, Jiangyan Jin, Jialing Bao, Zeyang Zhou
Summary: This study found that microsporidia infection promotes the maturation of monocytes and their extravasation from peripheral blood to inflammation sites. This is the first systematic analysis of monocyte maturation and trafficking during microsporidia infection, providing a better understanding of the pathogen-host interactions.
Article
Multidisciplinary Sciences
Chengyuan Cai, Danning Zeng, Qing Gao, Lei Ma, Bohang Zeng, Yi Zhou, He Wang
Summary: The study revealed that downregulation of FPN1 in hepatocytes increases the number of macrophages, promoting their proliferation and polarization, leading to hepatic fibrosis. The FPN1 axis could potentially serve as a target for treating hepatic fibrosis.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Shasha Yin, Liu Liu, Lauren E. Ball, Yalong Wang, Mark T. Bedford, Stephen A. Duncan, Haizhen Wang, Wenjian Gan
Summary: This study identifies protein arginine methyltransferase 1 (PRMT1) as a critical regulator of the GATOR2 complex, which activates the mTORC1 pathway for tumor growth. Inhibition of the CDK5-PRMT1-WDR24 axis suppresses HCC cell proliferation and tumor growth. High PRMT1 protein expression is associated with elevated mTORC1 signaling in patients with HCC.
Article
Cell Biology
Chan Mo, Shuwen Xie, Bin Liu, Weichao Zhong, Ting Zeng, Sha Huang, Yuqi Lai, Guanghui Deng, Chuying Zhou, Weixin Yan, Yuyao Chen, Shaohui Huang, Lei Gao, Zhiping Lv
Summary: The study demonstrates that IDO1 affects the recruitment of immune cells by inhibiting DCs maturation and subsequent T cell proliferation, exacerbating the progression of liver fibrosis. Targeting IDO1 to ameliorate immune responses in the hepatic and splenic microenvironment may be essential for therapeutic effects on liver fibrosis.
CELL DEATH & DISEASE
(2021)
Article
Immunology
Yingxia Zheng, Zheyi Chen, Bingqian Zhou, Shiyu Chen, Li Han, Ningdai Chen, Yanhui Ma, Guohua Xie, Junyao Yang, Hong Nie, Lisong Shen
Summary: This study found that PRMT5 is important for T cell subset differentiation and antitumor immunity. PRMT5 deficiency promotes CD8(+) T cell differentiation into terminal effector cells and impairs the transition to memory precursor cells. Additionally, PRMT5 deficiency accelerates tumor progression.
JOURNAL OF IMMUNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Xun Zhou, Hui Chen, Jinqing Li, Yingfeng Shi, Shougang Zhuang, Na Liu
Summary: Methylation can affect both histones and non-histones, with increased expression and activity of lysine and arginine methyltransferases in kidney diseases. Inhibiting most methyltransferases can alleviate kidney injury, but the role of methyltransferases in different animal models remains controversial.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Zachary A. Hing, Janek S. Walker, Ethan C. Whipp, Lindsey Brinton, Matthew Cannon, Pu Zhang, Steven Sher, Casey B. Cempre, Fiona Brown, Porsha L. Smith, Claudio Agostinelli, Stefano A. Pileri, Jordan N. Skinner, Katie Williams, Hannah Phillips, Jami Shaffer, Larry P. Beaver, Alexander Pan, Kyle Shin, Charles T. Gregory, Gulcin H. Ozer, Selen A. Yilmaz, Bonnie K. Harrington, Amy M. Lehman, Lianbo Yu, Vincenzo Coppola, Pearlly Yan, Peggy Scherle, Min Wang, Philip Pitis, Chaoyi Xu, Kris Vaddi, Selina Chen-Kiang, Jennifer Woyach, James S. Blachly, Lapo Alinari, Yiping Yang, John C. Byrd, Robert A. Baiocchi, Bradley W. Blaser, Rosa Lapalombella
Summary: PRMT5 plays an important role in the progression of CLL to Richter transformation, and the PRMT5 inhibitor PRT382 shows potential therapeutic value.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Yu Lei, Ping Han, Yu Chen, Han Wang, Shuhui Wang, Muru Wang, Jingmei Liu, Wei Yan, Dean Tian, Mei Liu
Summary: PRMT3 is upregulated in HCC, and its overexpression promotes HCC cell proliferation, glycolysis, and tumor growth. PRMT3 interacts with LDHA and mediates ADMA modification at the R112 site.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Biochemical Research Methods
Antoine Lesur, Francois Bernardin, Ois Bernardin, Eric Koncina, Elisabeth Letellier, Gary Kruppa, Pierre-Olivier Schmit, Gunnar Dittmar
Summary: Blood analysis is a crucial part of clinical diagnostics, and mass spectrometry has made significant advancements in protein analysis in blood samples. The prm-PASEF method combines ion mobility as an additional separation dimension, increasing proteome coverage and allowing for shorter chromatographic gradients. An alternative method, g-dia-PASEF, is described as a less time-consuming approach.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Medicine, General & Internal
Jan Van Slambrouck, Mona Khan, Erik Verbeken, Sumin Choi, Vincent Geudens, Cedric Vanluyten, Simon Feys, Emiel Vanhulle, Elke Wollants, Kurt Vermeire, Charlotte De Fays, Lucia Aversa, Janne Kaes, Dirk Van Raemdonck, Robin Vos, Bart Vanaudenaerde, Gert De Hertogh, Els Wauters, Joost Wauters, Laurens J. Ceulemans, Peter Mombaerts
Summary: A study introduces a methodology for visualizing the replication of SARS-CoV-2 in the lungs at single-cell resolution. The method utilizes fluorescence staining and confocal imaging to observe viral replication in histological sections. This technique can be valuable for studying SARS-CoV-2 variants and other respiratory viruses.
Article
Biochemical Research Methods
Daniel Perez-Hernandez, Liza Filali, Clement Thomas, Gunnar Dittma
Summary: Here, a protocol is presented to identify and quantify phosphopeptides during the dynamic formation of an immunological synapse. The steps include mixing isotope-labeled immune and target cells, stabilizing cell-to-cell conjugates by cross-linking, and isolating them by fluorescence-activated cell sorting. The protocol also involves the isolation of phosphopeptides by phosphopeptide enrichment and their measurement by mass spectrometry, followed by analysis of the data for cell-specific phosphopeptides using isotope labeling and label-free quantification.
Review
Chemistry, Multidisciplinary
Yuan Xiong, Qian Feng, Li Lu, Xingan Qiu, Samuel Knoedler, Adriana Christine Panayi, Dongsheng Jiang, Yuval Rinkevich, Ze Lin, Bobin Mi, Guohui Liu, Yanli Zhao
Summary: This article introduces the application of metal-organic frameworks (MOFs) and their composites in the treatment of chronic wounds. MOFs have low toxicity, high eco-friendliness, and exhibit antibacterial effects and pro-angiogenic activity. The article also highlights the potential of MOFs' multifunctionality and customizability in clinical therapy.
ADVANCED MATERIALS
(2023)
Article
Respiratory System
Vincent Geudens, Jan Van Slambrouck, Gitte Aerts, Lynn Willems, Tinne Goos, Janne Kaes, Andrea Zajacova, Iwein Gyselinck, Celine Aelbrecht, Astrid Vermaut, Hanne Beeckmans, Marie Vermant, Charlotte De Fays, Annelore Sacreas, Lucia Aversa, Michaela Orlitova, Arno Vanstapel, Ivan Josipovic, Matthieu N. Boone, John E. McDonough, Birgit Weynand, Charles Pilette, Wim Janssens, Lieven Dupont, Wim A. Wuyts, Geert M. Verleden, Dirk E. van Raemdonck, Robin Vos, Ghislaine Gayan-Ramirez, Laurens J. Ceulemans, Bart M. Vanaudenaerde
Summary: This study assessed disease progression in COVID-19 patients using in vivo CT and ex vivo microCT, and described the changes in lung volume and density as well as airway morphology. Results showed that COVID-19 lung volumes decreased and densities increased over time, mainly in the lower lobes. Ex vivo microCT revealed a 60% reduction in lung volume compared to controls, and increased visibility of airways in COVID-19 lungs, possibly due to fibrosis and increased airway diameter. Small-partition microCT further showed deformations in airway morphology and heterogeneous fibrotic organization in severely affected regions, which was confirmed by histology.
JOURNAL OF THORACIC DISEASE
(2023)
Article
Respiratory System
Xin Jin, Cedric Vanluyten, Michaela Orlitova, Jan Van Slambrouck, Robin Vos, Geert M. Verleden, Laurent Godinas, Arne P. Neyrinck, Catherine Ingels, Bart M. Vanaudenaerde, Paul De Leyn, Hans Van Veer, Lieven Depypere, Yi Zhang, Dirk E. M. Van Raemdonck, Laurens J. Ceulemans
Summary: This study demonstrated the feasibility and safety of off-pump re-LTx avoiding clamshell incision in selected patients. Compared to the more invasive approaches, clamshell-avoiding off-pump re-LTx resulted in shorter operative time and lower incidence of grade 3 primary graft dysfunction.
JOURNAL OF THORACIC DISEASE
(2023)
Article
Public, Environmental & Occupational Health
M. Murat Oktay, Behcet Al, Mustafa Bogan, Seval Kul, Hasan Gumusboga, Hasan Bayram
Summary: This study investigates the impacts of desert dust storms, particulate matter PM10, and daily maximum temperature on mortality and emergency department visits due to stroke in the city of Gaziantep, Southeast Turkey. The results show that maximum temperature increases both emergency department visits and mortality due to stroke in women. Desert dust storms have a significant impact on emergency department visits, but no significant effect on mortality.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Biochemistry & Molecular Biology
Jie Zhang, Mark A. Keibler, Wentao Dong, Jenny Ghelfi, Thekla Cordes, Tamara Kanashova, Arnaud Pailot, Carole L. Linster, Gunnar Dittmar, Christian M. Metallo, Tim Lautenschlaeger, Karsten Hiller, Gregory Stephanopoulos
Summary: This study identifies erythronate as an accumulated metabolite in human cancer cell lines and identifies ALDH1A1 as the main enzyme involved in erythronate production. The findings suggest that erythronate accumulation may serve as a biomarker for certain types of cancer.
Article
Chemistry, Medicinal
Li Wu, Ning Yang, Meng Guo, Didi Zhang, Reza A. Ghiladi, Hasan Bayram, Jun Wang
Summary: Sound vibration is a natural stimulus that triggers physiological changes in plants, leading to the production of secondary metabolites. This review explores the potential role of sound stimulation in regulating the biosynthesis of secondary metabolites and the subsequent physiological changes in plants, focusing on transcriptional and epigenetic regulation. Various factors, such as plant species, growth stage, sound types, frequency, intensity, and exposure time, can influence the production of secondary metabolites. The review also discusses the biochemical and physiological changes induced by sound stimulation, as well as the limitations of previous studies and the future application of sound waves in plant secondary metabolite biosynthesis.
NATURAL PRODUCTS AND BIOPROSPECTING
(2023)
Meeting Abstract
Critical Care Medicine
C. De Fays, H. Beeckmans, P. Kerckhof, V. Geudens, A. Vermaut, I. Gyselinck, T. Goos, M. Vermant, J. Kaes, J. Van Slambrouck, Y. Mohamady, L. Willems, L. Aversa, K. Maes, C. Aelbrecht, S. Everaerts, J. E. Mcdonough, L. J. De Sadeleer, S. Gohy, J. Ambroise, W. A. Wuyts, W. L. Janssens, L. J. Ceulemans, D. E. M. Van Raemdonck, R. Vos, T. Hackett, J. C. Hogg, N. Kaminski, C. Pilette, G. Gayan-Ramirez, B. Vanaudenaerde
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Meeting Abstract
Critical Care Medicine
S. Gogaert, T. Goos, M. Vermant, M. S. Carlon, N. De Crem, L. De Sadeleer, E. De langhe, A. Dubbeldam, B. Vanaudenaerde, J. Verschakelen, B. Weynand, J. Yserbyt, A. Bertrand, M. De Vos, W. A. Wuyts
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Meeting Abstract
Critical Care Medicine
T. S. Adams, J. C. Schupp, A. Balayev, A. Justet, P. Sharma, G. DeIuliis, X. Yan, W. A. Wuyts, B. Vanaudenaerde, N. Kaminski
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Article
Multidisciplinary Sciences
Gang Liu, Tatt Jhong Haw, Malcolm R. Starkey, Ashleigh M. Philp, Stelios Pavlidis, Christina Nalkurthi, Prema M. Nair, Henry M. Gomez, Irwan Hanish, Alan CY. Hsu, Elinor Hortle, Sophie Pickles, Joselyn Rojas-Quintero, Raul San Jose Estepar, Jacqueline E. Marshall, Richard Y. Kim, Adam M. Collison, Joerg Mattes, Sobia Idrees, Alen Faiz, Nicole G. Hansbro, Ryutaro Fukui, Yusuke Murakami, Hong Sheng Cheng, Nguan Soon Tan, Sanjay H. Chotirmall, Jay C. Horvat, Paul S. Foster, Brian GG. Oliver, Francesca Polverino, Antonio Ieni, Francesco Monaco, Gaetano Caramori, Sukhwinder S. Sohal, Ken R. Bracke, Peter A. Wark, Ian M. Adcock, Kensuke Miyake, Don D. Sin, Philip M. Hansbro
Summary: TLR7 plays a crucial role in the development of emphysema and COPD through the modulation of mast cell activity. This finding highlights TLR7 as a potential therapeutic target for human lung inflammation.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Multidisciplinary Sciences
Yu Tao, Yuan Zhang, Xiaohong Jin, Nan Hua, Hong Liu, Renfei Qi, Zitong Huang, Yufang Sun, Dongsheng Jiang, Terrance P. Snutch, Xinghong Jiang, Jin Tao
Summary: This study uncovers an epigenetic pathway driven by microRNA regulation of beta-endorphin synthesis in hypothalamic arcuate nucleus (ARC) neurons to modulate neuropathic pain.
NATURE COMMUNICATIONS
(2023)