Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 268, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2021.120697
Keywords
Lactate dehydrogenase; Silicon quantum dots; Nicotinamide adenine dinucleotide; Melanoma
Categories
Funding
- National Science Foundation of China [8207104259, 22174163]
- Hunan Provincial Science and Technology Plan Project, China [2019TP1001]
- Innovation Driven Project of Central South University [2020CX002]
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Silicon quantum dots synthesized from 3-aminopropyltrimethoxysilane were used to detect lactate dehydrogenase activity through changes in fluorescence intensity. The assay showed high selectivity and linear calibration, successfully applied in analysis of LDH in human serum samples, with potential for diagnosis and prognosis in various diseases, especially melanoma.
Silicon quantum dots (SiQDs) synthesized based on 3-aminopropyltrimethoxysilane (ATPMS) as silicon source were used to detect the activity of lactate dehydrogenase (LDH) through changes of fluorescence intensity of SiQDs. In this system, the fluorescence of SiQDs was first quenched by nicotinamide adenine dinucleotide (NADH), and then recovered with the addition of LDH, as NADH was consumed by catalytic reaction of LDH. A linear calibration chart of LDH is obtained in the range of 0.77-385 U/mL. The assay displays high selectivity towards LDH detection, and was successfully applied to the analysis of LDH in human serum samples. This assay has great prospects for the diagnosis and prognosis of various diseases, especially melanoma. (C) 2021 Elsevier B.V. All rights reserved.
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