Review
Plant Sciences
Dilnur Tussipkan, Shuga A. Manabayeva
Summary: New breeding technologies, such as genome editing, have been used to modify genomes of important crops, including tuber crops like potato. The CRISPR/Cas system has been specifically applied to enhancing traits like self-incompatibility, abiotic-biotic resistance, nutrient content, and post-harvest factors in these crops, providing a foundation for future cultivar development.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Junye Jiang, Will Feindel, Kylie Swisher Grimm, Michael Harding, David Feindel, Stacey Bajema, Jie Feng
Summary: In this study, a loop-mediated isothermal amplification (LAMP) method was developed and evaluated for the detection of 'Candidatus Liberibacter solanacearum' (Lso) haplotypes A and B. The LAMP-A and LAMP-B primers showed high specificity to Lso and generated positive signals only from Lso-infected tomato DNA mixtures. The researchers highly recommend the LAMP-A and LAMP-B primers in combination with the LAMP technique for rapid, accurate, and direct differentiation of Lso haplotypes A and B.
Article
Plant Sciences
Xuejin Cui, Kehong Liu, Jie Huang, Shimin Fu, Qingdong Chen, Xu Liu, Changyong Zhou, Xuefeng Wang
Summary: This study evaluated the transmission route of citrus huanglongbing (HLB) in newly invaded regions of Sichuan, China and proposed strategies for preventing its spread. The results showed higher molecular variability of HLB in the newly invaded area compared to the endemic areas, as well as diversity in the mitochondrial genomes of the Asian citrus psyllid (ACP).
Review
Microbiology
Ruben D. Arroyo-Olarte, Ricardo Bravo Rodriguez, Edgar Morales-Rios
Summary: CRISPR-Cas technology has revolutionized genome editing in eukaryotic organisms with its simplicity and programmability, but faces challenges in bacterial genome editing. Alternative approaches are being explored to overcome these issues and expand the application of genome editing in bacteria.
Article
Engineering, Environmental
Xuhan Deng, Jing Yuan, Liping Chen, Hang Chen, Chaohai Wei, Per H. Nielsen, Stefan Wuertz, Guanglei Qiu
Summary: This study systematically analyzed the occurrence and characteristics of CRISPR-Cas systems and prophages in Ca. Accumulibacter members. The results showed that 28 Ca. Accumulibacter genomes encode CRISPR-Cas systems, providing adaptive resistance to phage predation. The study also revealed high relative abundances of Ca. Accumulibacter with CRISPR-Cas systems in laboratory-scale EBPR bioreactors, indicating their specific resistance to phages.
Article
Microbiology
Ching-Ting Huang, Shu-Ting Cho, Yu-Chen Lin, Choon-Meng Tan, Yi-Ching Chiu, Jun-Yi Yang, Chih-Horng Kuo
Summary: This study obtained the complete genome sequence of a phytoplasma associated with witches' broom disease of loofah and found that the genome has remarkably high repetitiveness. The study also classified the phytoplasma-specific mobile genetic elements into three types and showed their significance in phytoplasma genome variability.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Bowei Yuan, Congcong Yuan, Lulu Li, Miao Long, Zeliang Chen
Summary: This article reviews the application of the CRISPR/Cas system in pathogen detection, focusing on the Type II (cas9), Type V (cas12a, cas12b, cas14a), and Type VI (cas13a) systems. The CRISPR/Cas system offers strong specificity, high sensitivity, and convenience for detection, making it suitable for practical applications.
Article
Plant Sciences
Xing Ma, Keith L. L. Perry, Bryan Swingle
Summary: In this study, a recent strain of Clavibacter sepedonicus, K496, was isolated from potato tubers with vascular cylinder or pith tissue discolorations. The genome sequence of K496 was de novo assembled using Nanopore reads and polished with Illumina reads. The resulting K496 genome consists of a circular chromosome of 3,266,016 bp and a linear plasmid of 135,489 bp. Bioinformatics analysis predicted a total of 3,301 genes in the K496 genome, including protein-coding genes, pseudogenes, rRNA-coding genes, tRNAs, and noncoding RNAs.
Review
Biochemistry & Molecular Biology
Nicolas Ayub, Gabriela Soto
Summary: The CRISPR/Cas system is an efficient tool for editing plant genomes, but technical limitations and the unique characteristics of plants impact the spread of its benefits in agriculture. Effort and selection for edited traits are comparable to transgenic equivalents, and dominant mutations on single-copy genes may accelerate their integration into varieties. Public grants for edited variety productions can be considered to boost feasibility and address challenges in crop production due to climate change.
Article
Plant Sciences
Spela Alic, Marina Dermastia, Johan Burger, Matthew Dickinson, Gerhard Pietersen, Gert Pietersen, Tanja Dreo
Summary: A specific loop-mediated isothermal amplification (LAMP) assay was developed and validated for the detection of a distinct strain of grapevine 'Candidatus Phytoplasma asteris' in South Africa. The LAMP assays targeting the ftsH/hflB gene region showed good sensitivity and reproducibility. This study demonstrates the importance of a genome-informed molecular test design and an efficient validation approach for the detection of phytoplasmas in regions with limited reference and sample materials.
Review
Immunology
Yizheng Qian, Dapeng Zhou, Min Li, Yongxiang Zhao, Huanhuan Liu, Li Yang, Zhiqin Ying, Guangtao Huang
Summary: Antimicrobial-resistant ESKAPE pathogens pose a global threat to human health, and finding innovative therapeutic strategies to combat them is crucial. The CRISPR system has gained attention for its high specificity, but there is currently no direct CRISPR-based treatment. This review explores the applications of the CRISPR-Cas system in studying ESKAPE pathogens, aiming to guide research on novel drugs and addressing the issues caused by multidrug-resistant bacteria.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Plant Sciences
Cesar A. Reyes-Corral, W. Rodney Cooper, Alexander Karasev, Carolina Delgado-Luna, Sergio R. Sanchez-Pena
Summary: The study confirmed that the foliar symptoms observed on tomatillo in an experimental plot near Saltillo, Mexico were caused by infection of 'Ca. L. solanacearum', rather than psyllid yellows. The majority of infected plants and psyllids harbored haplotype B, with one psyllid also infected with haplotype A. Potato psyllids spread 'Ca. L. solanacearum' in plants, and the infection was associated with increased plant diversity in their diet.
Review
Genetics & Heredity
Xin Hou, Xiaomeng Guo, Yan Zhang, Qiang Zhang
Summary: Potato is a crucial non-cereal food crop and plays a vital role in food security. The CRISPR/Cas system, with its easy operation, high efficiency, and low cost, shows potential in potato breeding. This paper reviews the mechanism, derivative types, and applications of the CRISPR/Cas system in improving potato quality, resistance, and overcoming self-incompatibility.
FRONTIERS IN GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Aytug Tuncel, Yiping Qi
Summary: Genome engineering has revolutionized plant biotechnology and agriculture, and the use of gene editing techniques has made crop improvement easier, faster, and more precise. However, potato, an important crop for global food security, has not fully benefited from these techniques due to its unique genetic features. Genome editing provides a powerful tool for trait improvement in potato, but technical challenges and unexplored applications remain.
Article
Plant Sciences
Yelin Li, Zhiqing Tan, Xiaolan Wang, Liping Hou
Summary: In this study, the metabolic changes in Candidatus Liberibacter solanacearum (CLso)-infected potato psyllids were analyzed. A total of 34 potential biomarkers related to amino acid, carbohydrate, and lipid metabolism were identified. The findings provide valuable biochemical information and offer a theoretical basis for a better understanding of psyllid-pathogen interactions, potentially aiding in breaking the transmission cycle of these diseases.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Kabin Xie, Bastian Minkenberg, Yinong Yang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Biochemistry & Molecular Biology
Kabin Xie, Jianping Chen, Qin Wang, Yinong Yang
Letter
Biotechnology & Applied Microbiology
Bastian Minkenberg, Jianwei Zhang, Kabin Xie, Yinong Yang
PLANT BIOTECHNOLOGY JOURNAL
(2019)
Editorial Material
Biotechnology & Applied Microbiology
Kutubuddin A. Molla, Yinong Yang
TRENDS IN BIOTECHNOLOGY
(2020)
Article
Plant Sciences
Matthew S. Wheatley, Yinong Yang
Summary: The CRISPR/Cas system, as a powerful genome editing tool, has great potential in plant pathology and disease management, with wide applications in plant-pathogen interactions research, editing of disease resistance genes in plants, and molecular diagnosis of pathogens.
Article
Plant Sciences
Matthew S. Wheatley, Yong-Ping Duan, Yinong Yang
Summary: DETECTR assay using Cas12a technology has been developed for highly specific and sensitive detection of CLas nucleic acids from infected samples. This technology enables detection of CLas at attomolar level and shows compatibility with lateral flow assay for potential field-deployable diagnosis. The improvements in detection sensitivity and flexibility of the DETECTR technology position it as a potentially suitable tool for early detection of CLas in infected regions.
Article
Plant Sciences
Jenna E. Reeger, Matthew Wheatley, Yinong Yang, Kathleen M. Brown
Summary: By utilizing CRISPR-Cas9 genome editing, researchers identified three transcription factor candidate genes that potentially regulate metaxylem vessel size and number in rice. The study found that these candidate genes may affect drought tolerance traits in rice under well-watered and drought conditions.
