Going beyond the limit: Increasing global translation activity leads to increased productivity of recombinant secreted proteins in Pichia pastoris

Production of Industrial Enzymes via Pichia pastoris as a Cell Factory in Bioreactor: Current Status and Future Aspects

(2021) Zeynep Efsun Duman-Özdamar et al.   PROTEIN JOURNAL

Treatment with surfactants enables quantification of translational activity by O-propargyl-puromycin labelling in yeast

(2021) Jennifer Staudacher et al.   BMC MICROBIOLOGY

The Yeast eIF2 Kinase Gcn2 Facilitates H 2 O 2 -Mediated Feedback Inhibition of Both Protein Synthesis and Endoplasmic Reticulum Oxidative Folding during Recombinant Protein Production

(2021) Veronica Gast et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Characterization of methanol utilization negative Pichia pastoris for secreted protein production: New cultivation strategies for current and future applications

(2020) Domen Zavec et al.   BIOTECHNOLOGY AND BIOENGINEERING

Engineered transient and stable overexpression of translation factors eIF3i and eIF3c in CHOK1 and HEK293 cells gives enhanced cell growth associated with increased c-Myc expression and increased recombinant protein synthesis

(2020) Anne Roobol et al.   METABOLIC ENGINEERING

Pseudohyphal differentiation in Komagataella phaffii: investigating the FLO gene family

(2020) Sonakshi De et al.   FEMS YEAST RESEARCH

Downregulation of eukaryotic translation initiation factor 3b inhibited proliferation and metastasis of gastric cancer

(2019) Fang Ma et al.   Cell Death & Disease

Genomic diversity and meiotic recombination among isolates of the biotech yeast Komagataella phaffii (Pichia pastoris)

(2019) Stephanie Braun-Galleani et al.   Microbial Cell Factories

Superior proteina titers in half the fermentation time: Promoter and process engineering for the glucose-regulated GTH1 promoter of Pichia pastoris

(2018) Roland Prielhofer et al.   BIOTECHNOLOGY AND BIOENGINEERING

Origins of robustness in translational control via eukaryotic translation initiation factor (eIF) 2

(2018) Mohammad Farhan Khan et al.   JOURNAL OF THEORETICAL BIOLOGY

High level expression and purification of recombinant human serum albumin in Pichia pastoris

(2018) Wen Zhu et al.   PROTEIN EXPRESSION AND PURIFICATION

Translational regulation in response to stress in Saccharomyces cerevisiae

(2018) Robert A. Crawford et al.   YEAST

Role of eIF2α Kinases in Translational Control and Adaptation to Cellular Stress

(2018) Ronald C. Wek   Cold Spring Harbor Perspectives in Biology

Protein Synthesis Initiation in Eukaryotic Cells

(2018) William C. Merrick et al.   Cold Spring Harbor Perspectives in Biology

Non-invasive measurement of mRNA decay reveals translation initiation as the major determinant of mRNA stability

(2018) Leon Y Chan et al.   eLife

Embraced by eIF3: structural and functional insights into the roles of eIF3 across the translation cycle

(2017) Leoš Shivaya Valášek et al.   NUCLEIC ACIDS RESEARCH

Recombinant pharmaceuticals from microbial cells: a 2015 update

(2016) Laura Sanchez-Garcia et al.   Microbial Cell Factories

The ribosome in action: Tuning of translational efficiency and protein folding

(2016) Marina V. Rodnina   PROTEIN SCIENCE

Biological insights into the expression of translation initiation factors from recombinant CHOK1SV cell lines and their relationship to enhanced productivity

(2015) E. J. Mead et al.   BIOCHEMICAL JOURNAL

Cultivation strategies to enhance productivity of Pichia pastoris: A review

(2015) V. Looser et al.   BIOTECHNOLOGY ADVANCES

Systems-level organization of yeast methylotrophic lifestyle

(2015) Hannes Rußmayer et al.   BMC BIOLOGY

Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level

(2015) Roland Prielhofer et al.   BMC GENOMICS

The cellular growth rate controls overall mRNA turnover, and modulates either transcription or degradation rates of particular gene regulons

(2015) José García-Martínez et al.   NUCLEIC ACIDS RESEARCH

Probing the closed-loop model of mRNA translation in living cells

(2015) Stuart K Archer et al.   RNA Biology

Global mRNA selection mechanisms for translation initiation

(2015) Joseph Costello et al.   GENOME BIOLOGY

Pichia pastorissecretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteins

(2014) Andreas Maccani et al.   Biotechnology Journal

Correlation of cell growth and heterologous protein production by Saccharomyces cerevisiae

(2013) Zihe Liu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

InPichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response

(2013) Corinna Rebnegger et al.   Biotechnology Journal

Pichia pastoris: protein production host and model organism for biomedical research

(2013) Brigitte Gasser et al.   Future Microbiology

Translation Initiation Requires Cell Division Cycle 123 (Cdc123) to Facilitate Biogenesis of the Eukaryotic Initiation Factor 2 (eIF2)

(2013) Angelika F. Perzlmaier et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Disruption of YPS1 and PEP4 genes reduces proteolytic degradation of secreted HSA/PTH in Pichia pastoris GS115

(2013) Min Wu et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Unconventional microbial systems for the cost-efficient production of high-quality protein therapeutics

(2012) José Luis Corchero et al.   BIOTECHNOLOGY ADVANCES

A Novel Strategy for Selection and Validation of Reference Genes in Dynamic Multidimensional Experimental Design in Yeast

(2012) Ayca Cankorur-Cetinkaya et al.   PLoS One

The Elongation, Termination, and Recycling Phases of Translation in Eukaryotes

(2012) T. E. Dever et al.   Cold Spring Harbor Perspectives in Biology

The Mechanism of Eukaryotic Translation Initiation: New Insights and Challenges

(2012) A. G. Hinnebusch et al.   Cold Spring Harbor Perspectives in Biology

Reverse engineering of protein secretion by uncoupling of cell cycle phases from growth

(2011) Markus Buchetics et al.   BIOTECHNOLOGY AND BIOENGINEERING

Depletion of eIF4G from yeast cells narrows the range of translational efficiencies genome-wide

(2011) Eun-Hee Park et al.   BMC GENOMICS

Assessing the Components of the eIF3 Complex and their Phosphorylation Status

(2011) Adam R. Farley et al.   JOURNAL OF PROTEOME RESEARCH

Glucose depletion inhibits translation initiation via eIF4A loss and subsequent 48S preinitiation complex accumulation, while the pentose phosphate pathway is coordinately up-regulated

(2011) Lydia M. Castelli et al.   MOLECULAR BIOLOGY OF THE CELL

Identification and characterisation of novel Pichia pastoris promoters for heterologous protein production

(2010) Gerhard Stadlmayr et al.   JOURNAL OF BIOTECHNOLOGY

The mechanism of eukaryotic translation initiation and principles of its regulation

(2010) Richard J. Jackson et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

The Effect of Temperature on the Proteome of RecombinantPichia pastoris

(2009) Martin Dragosits et al.   JOURNAL OF PROTEOME RESEARCH

Genome sequence of the recombinant protein production host Pichia pastoris

(2009) Kristof De Schutter et al.   NATURE BIOTECHNOLOGY

Multiway real-time PCR gene expression profiling in yeast Saccharomyces cerevisiae reveals altered transcriptional response of ADH-genes to glucose stimuli

(2008) Anders Ståhlberg et al.   BMC GENOMICS

Requirement of RNA Binding of Mammalian Eukaryotic Translation Initiation Factor 4GI (eIF4GI) for Efficient Interaction of eIF4E with the mRNA Cap

(2008) A. Yanagiya et al.   MOLECULAR AND CELLULAR BIOLOGY

Purification of Recombinant Human Serum Albumin (rHSA) Produced by Genetically ModifiedPichia Pastoris

(2008) M. Belew et al.   SEPARATION SCIENCE AND TECHNOLOGY