Antibacterial activity of silver nanoparticles phytosynthesized from Glochidion candolleanum leaves

In the present study, Glochidion candolleanum (Wight & Arn.) Chakrab. & M. Gangop. leaf (GCL)-ethyl acetate extract was used for the phytosynthesis of silver nanoparticles (AgNPs) and subjected to antibacterial assay with G(+ve) and G(-ve) bacteria. The GCL center dot AgNPs synthesized were characterized through various spectroscopic and microscopic analyses, while the phytochemical compounds was analyzed by GC-MS. The discrete GCL center dot AgNPs formed were micron-scale range. TEM images of AgNPs reveal that nanoparticles were spherical and ellipsoidal in shape, dispersed uniformly and capped with secondary material of approximate to 10 nm thickness. A maximum inhibition zone of 12.2 mm diameter was observed against Salmonella enterica followed by 11.8 mm diameter against Pseudomonas aeruginosa. GC-MS analysis revealed the occurrence of bioactive phytocompounds in GCL extract, hexamethylcyclotrisiloxane, 2,4,6-cycloheptatrien, benzenepropanoic acid, 2-ethylacridine, benzo[h]quinoline, methyltris(trimethylsiloxy)silane and 5-methyl-2-phenylindolizine. The findings suggest that GCL could be used for developing antibacterial AgNPs against pathogenic bacteria.

Automated summary

In this study, silver nanoparticles were synthesized using the ethyl acetate extract of Glochidion candolleanum leaves and showed strong antibacterial activity against Salmonella enterica and Pseudomonas aeruginosa. GC-MS analysis revealed the presence of various bioactive phytochemicals in the extract.