A sensitive visual method for onsite detection of quarantine pathogenic bacteria from horticultural crops using an LbCas12a variant system

Design of a diagnostic system based on molecular markers derived from the ascomycetes pan-genome analysis: The case of Fusarium dieback disease

(2021) Mirna Vázquez-Rosas-Landa et al.   PLoS One

New lateral flow immunoassay for on-site detection of Erwinia amylovora and its application on various organs of infected plants

(2021) S.C. Razo et al.   PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY

An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing

(2021) Kean Hean Ooi et al.   Nature Communications

Integrating taxonomic, functional, and strain-level profiling of diverse microbial communities with bioBakery 3

(2021) Francesco Beghini et al.   eLife

Signal amplification and output of CRISPR/Cas-based biosensing systems: A review

(2021) Si-Yuan Wang et al.   ANALYTICA CHIMICA ACTA

An enhanced method for nucleic acid detection with CRISPR-Cas12a using phosphorothioate modified primers and optimized gold-nanopaticle strip

(2021) Jiaojiao Gong et al.   Bioactive Materials

Evaluation of CRISPR/Cas12a-based DNA detection for fast pathogen diagnosis and GMO test in rice

(2020) Yun-mu Zhang et al.   MOLECULAR BREEDING

Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases

(2020) Eszter Tóth et al.   NUCLEIC ACIDS RESEARCH

Application of droplet digital PCR in detection of seed-transmitted pathogen Acidovorax citrulli

(2020) Yu LU et al.   Journal of Integrative Agriculture

Integration of logic gates to CRISPR/Cas12a system for rapid and sensitive detection of pathogenic bacterial genes

(2020) Lei Peng et al.   ANALYTICA CHIMICA ACTA

CRISPR–Cas12-based detection of SARS-CoV-2

(2020) James P. Broughton et al.   NATURE BIOTECHNOLOGY

Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics

(2020) Shuhan Lu et al.   Cell Discovery

Selective endpoint visualized detection of Vibrio parahaemolyticus with CRISPR/Cas12a assisted PCR using thermal cycler for on-site application

(2020) Mengyao Zhang et al.   TALANTA

Cas12a-Activated Universal Field-Deployable Detectors for Bacterial Diagnostics

(2020) Yun Sun et al.   ACS Omega

Field detection of multiple RNA viruses/viroids in apple using a CRISPR/Cas12a‐based visual assay

(2020) Jian Jiao et al.   PLANT BIOTECHNOLOGY JOURNAL

Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection

(2020) Long T. Nguyen et al.   Nature Communications

Mechanistic Insights into the cis- and trans-Acting DNase Activities of Cas12a

(2019) Daan C. Swarts et al.   MOLECULAR CELL

Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing

(2019) Benjamin P. Kleinstiver et al.   NATURE BIOTECHNOLOGY

Fire blight disease, a fast-approaching threat to apple and pear production in China

(2019) Yu-qiang ZHAO et al.   Journal of Integrative Agriculture

Potential application of CRISPR-Cas12a fluorescence assay coupled with rapid nucleic acid amplification for detection of white spot syndrome virus in shrimp

(2019) Thawatchai Chaijarasphong et al.   AQUACULTURE

Uracil-Mediated New Photospacer-Adjacent Motif of Cas12a To Realize Visualized DNA Detection at the Single-Copy Level Free from Contamination

(2019) Cheng Qian et al.   ANALYTICAL CHEMISTRY

Naked-Eye Detection of Grapevine Red-Blotch Viral Infection Using a Plasmonic CRISPR Cas12a Assay

(2019) Yongya Li et al.   ANALYTICAL CHEMISTRY

SHERLOCK: nucleic acid detection with CRISPR nucleases

(2019) Max J. Kellner et al.   Nature Protocols

Contamination-free visual detection of CaMV35S promoter amplicon using CRISPR/Cas12a coupled with a designed reaction vessel: Rapid, specific and sensitive

(2019) Hui Wu et al.   ANALYTICA CHIMICA ACTA

Two-step genomic sequence comparison strategy to design Trichoderma strain-specific primers for quantitative PCR

(2019) Yang Zhang et al.   AMB Express

Review: The future of plant pathogen diagnostics in a nursery production system

(2019) Alka Rani et al.   BIOSENSORS & BIOELECTRONICS

Recombinase polymerase amplification applied to plant virus detection and potential implications

(2018) Binoy Babu et al.   ANALYTICAL BIOCHEMISTRY

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Development of a recombinase polymerase based isothermal amplification combined with lateral flow assay (HLB-RPA-LFA) for rapid detection of "Candidatus Liberibacter asiaticus"

(2018) Dilip Kumar Ghosh et al.   PLoS One

Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1

(2017) Takashi Yamano et al.   MOLECULAR CELL

Engineered Cpf1 variants with altered PAM specificities

(2017) Linyi Gao et al.   NATURE BIOTECHNOLOGY

Self-paired monoclonal antibody lateral flow immunoassay strip for rapid detection of Acidovorax avenae subsp. citrulli

(2016) Haijuan Zeng et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

In vivo high-throughput profiling of CRISPR–Cpf1 activity

(2016) Hui K Kim et al.   NATURE METHODS

PCR inhibitors - occurrence, properties and removal

(2012) C. Schrader et al.   JOURNAL OF APPLIED MICROBIOLOGY

Acidovorax citrulli: generating basic and applied knowledge to tackle a global threat to the cucurbit industry

(2012) SAUL BURDMAN et al.   MOLECULAR PLANT PATHOLOGY

Improved fireblight diagnostics using quantitative real-time PCR detection ofErwinia amylovorachromosomal DNA

(2009) M. Pirc et al.   PLANT PATHOLOGY

Duplex Real-Time Polymerase Chain Reaction Reveals Competition BetweenErwinia amylovoraandE. pyrifoliaeon Pear Blossoms

(2008) Susan M. Lehman et al.   PHYTOPATHOLOGY