Article
Anatomy & Morphology
Sheridan Sargent, Anna Brennan, Jessica Kennett Clark
Summary: By optimizing the hyperglycemic-induction protocol, we established a more accurate model of motor nerve degeneration similar to that seen in mammals. Our findings demonstrate the regenerative potential of motor nerve components following recovery from hyperglycemia, but persistent behavioral deficits. Additionally, we identified significant impairments in skeletal development, highlighting the global impact of hyperglycemia.
DEVELOPMENTAL DYNAMICS
(2023)
Article
Cell Biology
Vishnu Muraleedharan Saraswathy, Lili Zhou, Anthony R. McAdow, Brooke Burris, Deepika Dogra, Sven Reischauer, Mayssa H. Mokalled
Summary: This study reveals the dynamic expression of mstnb in dorsal spinal cord progenitors in zebrafish after injury. Mstnb mutants show impaired functional recovery, but normal glial and axonal bridging, and an increase in the number of newborn neurons at the lesion site. Treatment with human FGF1 rescues the molecular and cellular phenotypes of mstnb mutants.
Article
Neurosciences
Shivangi Gupta, Poonam Sharma, Mansi Chaudhary, Sharanya Premraj, Simran Kaur, Vijithkumar Vijayan, Manas Geeta Arun, Nagaraj Guru Prasad, Rajesh Ramachandran
Summary: Unlike mammals, zebrafish have the ability to regenerate damaged retina. This study reveals the importance of Phosphatase and tensin homolog (Pten) in zebrafish retina regeneration and identifies multiple signaling pathways involved in Pten's regulation of retinal cell proliferation.
Article
Neurosciences
Annelies Van Dyck, Ilse Bollaerts, An Beckers, Sophie Vanhunsel, Nynke Glorian, Jessie van Houcke, Tjakko J. van Ham, Lies De Groef, Lien Andries, Lieve Moons
Summary: The study demonstrates that inducing intraocular inflammation can promote axonal regrowth after optic nerve injury in zebrafish. Additionally, acute depletion of microglia and macrophages in the retina compromises optic nerve regeneration, highlighting the importance of altered glial cell interactions in the regenerative process.
Article
Developmental Biology
Debanjan Mukherjee, Ganesh Wagh, Mayssa H. Mokalled, Zacharias Kontarakis, Amy L. Dickson, Amey Rayrikar, Stefan Gunther, Kenneth D. Poss, Didier Y. R. Stainier, Chinmoy Patra
Summary: The study reveals that the gene ccn2a plays a crucial role in regulating heart regeneration in zebrafish after injury, by promoting cardiomyocyte proliferation and repopulation of damaged tissue. Additionally, Ccn2a enhances the expression of pro-regenerative extracellular matrix genes and inhibits the chemokine receptor gene cxcr3.1 to positively modulate the innate regenerative response of the adult zebrafish heart.
Article
Cell Biology
Raquel Lourenco, Ana S. Brandao, Jorge Borbinha, Rita Gorgulho, Antonio Jacinto
Summary: This study demonstrates that Yap plays a crucial role in regulating Muller glia response to injury, promoting cell cycle reentry and progenitor cell formation, leading to the differentiation of new photoreceptors in zebrafish retina regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell & Tissue Engineering
Susanna E. Riley, Yi Feng, Carsten Gram Hansen
Summary: Tissue regeneration capacity varies between mammals and lower vertebrates, with zebrafish showing robust regeneration abilities associated with the Hippo pathway. The effectors Yap/Taz of the Hippo pathway play a role in zebrafish regeneration, with potential therapeutic implications for enhancing regeneration in mammals.
NPJ REGENERATIVE MEDICINE
(2022)
Article
Developmental Biology
Lili Zhou, Anthony R. McAdow, Hunter Yamada, Brooke Burris, Dana Klatt Shaw, Kelsey Oonk, Kenneth D. Poss, Mayssa H. Mokalled
Summary: Unlike mammals, adult zebrafish have the ability to recover from major spinal cord injury. Glial cells in zebrafish play a pro-regenerative role in bridging the injured area, unlike reactive gliosis observed in mammalian spinal cord repair. This study provides insights into the molecular and cellular responses of glial cells after spinal cord injury in adult zebrafish, identifying key regulatory features and cellular progeny involved in innate spinal cord regeneration.
Article
Neurosciences
Jiwoon Lee, Bum-Kyu Lee, Jeffrey M. Gross
Summary: The zebrafish retina has strong regenerative potential, and Muller glia play a crucial role in this process. The transcriptional regulation by members of the Bromodomain (Brd) family is important for Muller glia-dependent retinal regeneration.
Article
Neurosciences
Aaron Krylov, Shuguang Yu, Kellie Veen, Axel Newton, Aojun Ye, Huiwen Qin, Jie He, Patricia R. Jusuf
Summary: In this study, single-cell RNA sequencing revealed heterogeneity in gene expression within quiescent Muller glia. Additionally, the researchers found a region-specific bias in the activation of Muller glia following selective ablation of different photoreceptor subtypes. These findings provide important insights into the molecular mechanisms of neural regeneration in the retina.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Cell Biology
Raquel R. Martins, Mazen Zamzam, Dhani Tracey-White, Mariya Moosajee, Ryan Thummel, Catarina M. Henriques, Ryan B. MacDonald
Summary: Ageing affects the regenerative capacity of Müller glia cells in the retina, but is insufficient to stimulate neurodegeneration. Müller glia cells undergo morphological aberrations and loss of vision with ageing.
Article
Cell Biology
Patrick Boyd, Leah J. Campbell, David R. Hyde
Summary: Zebrafish have the ability to regenerate lost neurons in the retina, which is mediated by Muller glia that divide and produce neuronal precursor cells. The early signals that induce this response are not well understood. Alternative ligands of the Ciliary neurotrophic factor receptor (CNTFR), such as Cardiotrophin-like cytokine factor 1 (Clcf1) and Cytokine receptor-like factor 1a (Crlf1a), are expressed in Muller glia of the light-damaged retina. These ligands are required for Muller glia proliferation in the damaged retina and have a neuroprotective effect. CLCF1/CRLF1 injection protects against rod photoreceptor cell death and induces rod precursor cell proliferation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell & Tissue Engineering
Stephanie L. Tsai, Steffany Villasenor, Rishita R. Shah, Jenna L. Galloway
Summary: Adult zebrafish tendons can activate and fully regenerate upon injury, unlike mammalian tendons. The regeneration process involves three main phases and ultimately restores normal structure. Pre-existing tenocytes are the main cellular source of regeneration, and TGF-β signaling plays a crucial role in cell recruitment and bridge formation. This study introduces the adult zebrafish tendon as a valuable comparative system for understanding regenerative mechanisms and inspiring new therapeutic strategies.
NPJ REGENERATIVE MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Jessica Schira-Heinen, Luzhou Wang, Seda Akguen, Sofia Blum, Brigida Ziegler, Andre Heinen, Hans-Peter Hartung, Patrick Kuery
Summary: Schwann cell transdifferentiation is crucial for peripheral nerve regeneration, but factors like chronic denervation can diminish repair capacity. The S1PR agonist Fingolimod enhances Schwann cell repair phenotype through activation of specific receptors, promoting neurite growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Maria Iribarne, David R. Hyde
Summary: This study investigated the role of microglia in zebrafish retinal regeneration after damage. The researchers found that acute and chronic damage led to different inflammation profiles and regeneration outcomes. Understanding the differences could provide valuable insights for improving therapeutic strategies for repairing injured mammalian tissues.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Neurosciences
Angela D. Morris, Gwendolyn M. Lewis, Sarah Kucenas
JOURNAL OF NEUROSCIENCE
(2017)
Review
Biochemistry & Molecular Biology
Taylor G. Welsh, Sarah Kucenas
JOURNAL OF NEUROCHEMISTRY
(2018)
Article
Multidisciplinary Sciences
Sho Morioka, Justin S. A. Perry, Michael H. Raymond, Christopher B. Medina, Yunlu Zhu, Liyang Zhao, Vlad Serbulea, Suna Onengut-Gumuscu, Norbert Leitinger, Sarah Kucenas, Jeffrey C. Rathmell, Liza Makowski, Kodi S. Ravichandran
Article
Biochemistry & Molecular Biology
Yunlu Zhu, Samantha C. Crowley, Andrew J. Latimer, Gwendolyn M. Lewis, Rebecca Nash, Sarah Kucenas
Article
Neurosciences
Stephan Raiders, Taeho Han, Nicole Scott-Hewitt, Sarah Kucenas, Deborah Lew, Mary A. Logan, Aakanksha Singhvi
Summary: The phagocytic activity of glial cells is crucial for proper nervous system development, maintenance, and health, with remarkably conserved pathways governing this engulfment process across different species and cell types.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biology
Laura Fontenas, Sarah Kucenas
Summary: MEP glia are derived from lateral floor plate precursors and require foxd3 for delamination and migration out of the spinal cord. Their development also depends on axonally derived neuregulin1, and overexpressing axonal cues can generate additional MEP glia in the spinal cord.
Article
Neurosciences
Melanie Piller, Inge L. Werkman, Evan A. Brown, Andrew J. Latimer, Sarah Kucenas
Summary: The study found that the ionotropic glutamate receptor subunit GluR4A plays a crucial role in OPC migration and myelination, disruption of which leads to abnormal migration and distribution of OPCs in the spinal cord. Through genetic and pharmacological experiments, it was discovered that voltage-gated calcium channels are downstream of glutamate receptor signaling in OPCs and can rescue migration and myelination defects caused by disrupted glutamate signaling.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Genetics & Heredity
Maria F. Ali, Andrew J. Latimer, Yinxue Wang, Leah Hogenmiller, Laura Fontenas, Adam J. Isabella, Cecilia B. Moens, Guoqiang Yu, Sarah Kucenas
Summary: The study demonstrates the essential role of Met signaling in the initial developmental migration of OPCs, showing that Met acts cell-autonomously in OPCs. These findings provide new insights into the regulation of OPC migration during development in vivo.
G3-GENES GENOMES GENETICS
(2021)
Editorial Material
Neurosciences
Ashtyn T. Wiltbank, Sarah Kucenas
Summary: The study revealed changes in diversity among peripheral glia during development and identified shared genetic profiles among mature glia. These findings provide new tools and pathways for understanding peripheral glial development and function.
TRENDS IN NEUROSCIENCES
(2021)
Article
Biology
Ashtyn T. Wiltbank, Emma R. Steisnon, Stacey J. Criswell, Melanie Piller, Sarah Kucenas
Summary: This study identifies Cd59 and developmental inflammation as key players in myelinating glial cell development, highlighting the collaboration between glia and the innate immune system to ensure normal neural development.
Article
Neurosciences
Kyla R. Hamling, Yunlu Zhu, Franziska Auer, David Schoppik
Summary: Animals utilize information about gravity and other destabilizing forces to maintain balance and navigate their environment. Measuring neural responses to these forces is technically complex and expensive. The authors propose a low-cost and noninvasive method called Tilt In Place Microscopy (TIPM) to measure neural activity during body orientation changes. Using TIPM, they studied vestibulospinal neurons in larval zebrafish and found reliable increases in activity in response to roll tilts. TIPM also allowed for longitudinal studies, revealing changes in response strength and neural representation. This method has important implications for understanding balance and neural development.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Cell Biology
Yunlu Zhu, Franziska Auer, Hannah Gelnaw, Samantha N. Davis, Kyla R. Hamling, Christina E. May, Hassan Ahamed, Niels Ringstad, Katherine I. Nagel, David Schoppik
Summary: Balance and movement impairment in neurological disorders can now be studied in more detail using the recently developed behavioral monitoring technology. However, the existing methods lack the necessary throughput and scalability for screening candidate genes and potential therapies. In this study, a scalable apparatus called SAMPL is presented, which can measure posture and locomotion in multiple organisms. Using SAMPL, researchers have discovered small but systematic variations in kinematic parameters between different genetic backgrounds in zebrafish, providing important data for screenings of balance and locomotor disorders.
Article
Anatomy & Morphology
Angela D. Morris, Alev Erisir, Stacey J. Criswell, Sarah Kucenas
DEVELOPMENTAL DYNAMICS
(2017)
Article
Neurosciences
Marija Adzic Bukvic, Danijela Laketa, Milorad Dragic, Irena Lavrnja, Nadezda Nedeljkovic
Summary: In neuroinflammatory states, functionally distinct eN/CD73 glycovariants appear, which may play a role in the development of the reactive astrocyte phenotype.
Correction
Neurosciences
L. Feng, H. Lo, Z. Hong, J. Zheng, Y. Yan, Z. Ye, X. Chen, X. Pan