Harnessing CRISPR-Cas to Combat COVID-19: From Diagnostics to Therapeutics

A Scalable, Easy-to-Deploy, Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material

(2021) Jennifer N. Rauch et al.   JOURNAL OF CLINICAL MICROBIOLOGY

A smartphone-read ultrasensitive and quantitative saliva test for COVID-19

(2021) Bo Ning et al.   Science Advances

Machine Learning-Driven and Smartphone-Based Fluorescence Detection for CRISPR Diagnostic of SARS-CoV-2

(2021) Aubin Samacoits et al.   ACS Omega

Nucleic Acid-Based Diagnostic Tests for the Detection SARS-CoV-2: An Update

(2021) Choo Yee Yu et al.   Diagnostics

Detection of SARS-CoV-2 and Its Mutated Variants via CRISPR-Cas13-Based Transcription Amplification

(2021) Yuxi Wang et al.   ANALYTICAL CHEMISTRY

One-tube SARS-CoV-2 detection platform based on RT-RPA and CRISPR/Cas12a

(2021) Yangyang Sun et al.   Journal of Translational Medicine

SARS-CoV-2 Direct Detection Without RNA Isolation With Loop-Mediated Isothermal Amplification (LAMP) and CRISPR-Cas12

(2021) Alfredo Garcia-Venzor et al.   Frontiers in Medicine

Carrying out pseudo dual nucleic acid detection from sample to visual result in a polypropylene bag with CRISPR/Cas12a

(2021) Hui Wu et al.   BIOSENSORS & BIOELECTRONICS

Current and innovative methods for the diagnosis of COVID‑19 infection (Review)

(2021) Luca Falzone et al.   INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

Gene signatures and potential therapeutic targets of Middle East respiratory syndrome coronavirus (MERS-CoV)-infected human lung adenocarcinoma epithelial cells

(2021) Yen-Hung Wu et al.   JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION

An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing

(2021) Kean Hean Ooi et al.   Nature Communications

CRISPR is a useful biological tool for detecting nucleic acid of SARS-CoV-2 in human clinical samples

(2021) Md. Rashidur Rahman et al.   BIOMEDICINE & PHARMACOTHERAPY

Sensitive and Easy-Read CRISPR Strip for COVID-19 Rapid Point-of-Care Testing

(2021) Hao Li et al.   CRISPR Journal

Genome Composition and Divergence of the Novel Coronavirus (2019-nCoV) Originating in China

(2020) Aiping Wu et al.   Cell Host & Microbe

Virus against virus: a potential treatment for 2019-nCov (SARS-CoV-2) and other RNA viruses

(2020) Tuan M. Nguyen et al.   CELL RESEARCH

RNA-Targeting CRISPR–Cas Systems and Their Applications

(2020) Michal Burmistrz et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Characteristics of and Important Lessons From the Coronavirus Disease 2019 (COVID-19) Outbreak in China

(2020) Zunyou Wu et al.   JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION

Presumed Asymptomatic Carrier Transmission of COVID-19

(2020) Yan Bai et al.   JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION

Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China

(2020) Chaolin Huang et al.   LANCET

Ultra-sensitive and high-throughput CRISPR-Powered COVID-19 diagnosis

(2020) Zhen Huang et al.   BIOSENSORS & BIOELECTRONICS

Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza

(2020) Timothy R. Abbott et al.   CELL

Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19

(2020) Daniel Blanco-Melo et al.   CELL

Massively multiplexed nucleic acid detection with Cas13

(2020) Cheri M. Ackerman et al.   NATURE

CRISPR–Cas12-based detection of SARS-CoV-2

(2020) James P. Broughton et al.   NATURE BIOTECHNOLOGY

A Trial of Lopinavir–Ritonavir in Adults Hospitalized with Severe Covid-19

(2020) Bin Cao et al.   NEW ENGLAND JOURNAL OF MEDICINE

Asymptomatic and Presymptomatic SARS-CoV-2 Infections in Residents of a Long-Term Care Skilled Nursing Facility — King County, Washington, March 2020

(2020) Anne Kimball et al.   MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT

Rapid and sensitive detection of COVID-19 using CRISPR/Cas12a-based detection with naked eye readout, CRISPR/Cas12a-NER

(2020) Xinjie Wang et al.   Science Bulletin

SARS-CoV-2 detection with CRISPR diagnostics

(2020) Lu Guo et al.   Cell Discovery

COVID-19 treatment: close to a cure? A rapid review of pharmacotherapies for the novel coronavirus (SARS-CoV-2)

(2020) Yang Song et al.   INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS

Sensitivity assessment of droplet digital PCR for SARS-CoV-2 detection

(2020) Luca Falzone et al.   INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

Estimating the effects of non-pharmaceutical interventions on COVID-19 in Europe

(2020) Seth Flaxman et al.   NATURE

SARS-CoV-2 pathophysiology and assessment of coronaviruses in CNS diseases with a focus on therapeutic targets

(2020) Jayalakshmi Vallamkondu et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

CRISPR/cas systems redefine nucleic acid detection: Principles and methods

(2020) Meng Wang et al.   BIOSENSORS & BIOELECTRONICS

Rapid, sensitive and specific SARS coronavirus-2 detection: a multi-center comparison between standard qRT-PCR and CRISPR based DETECTR

(2020) Eelke Brandsma et al.   JOURNAL OF INFECTIOUS DISEASES

CRISPR-based assays for rapid detection of SARS-CoV-2

(2020) Vivek S. Javalkote et al.   METHODS

Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

(2020) Julia Joung et al.   NEW ENGLAND JOURNAL OF MEDICINE

Mechanisms of SARS-CoV-2 transmission and pathogenesis

(2020) Andrew G. Harrison et al.   TRENDS IN IMMUNOLOGY

iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2

(2020) Zahir Ali et al.   VIRUS RESEARCH

Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection

(2020) Long T. Nguyen et al.   Nature Communications

Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay

(2020) Xiong Ding et al.   Nature Communications

A role for Biofoundries in rapid development and validation of automated SARS-CoV-2 clinical diagnostics

(2020) Michael A. Crone et al.   Nature Communications

Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19

(2020) Tieying Hou et al.   PLoS Pathogens

MeCas12a, a Highly Sensitive and Specific System for COVID‐19 Detection

(2020) Peixiang Ma et al.   Advanced Science

Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA

(2020) Maturada Patchsung et al.   Nature Biomedical Engineering

COVID‐19 diagnostic testing: Technology perspective

(2020) Meng Xu et al.   Clinical and Translational Medicine

Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR Technology

(2020) Bo Pang et al.   ANALYTICAL CHEMISTRY

Simultaneous Dual‐Gene Diagnosis of SARS‐CoV‐2 Based on CRISPR/Cas9‐Mediated Lateral Flow Assay

(2020) Erhu Xiong et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detection

(2020) Rui Wang et al.   BIOSENSORS & BIOELECTRONICS

Contamination-free visual detection of SARS-CoV-2 with CRISPR/Cas12a: A promising method in the point-of-care detection

(2020) Yanju Chen et al.   BIOSENSORS & BIOELECTRONICS

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

(2020) Parinaz Fozouni et al.   CELL

SARS-CoV-2 pathophysiology and its clinical implications: An integrative overview of the pharmacotherapeutic management of COVID-19

(2020) Aristides Tsatsakis et al.   FOOD AND CHEMICAL TOXICOLOGY

Immunologic testing for SARS-CoV-2 infection from the antigen perspective

(2020) Dandan Li et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2

(2020) Jon Arizti-Sanz et al.   Nature Communications

Rapid detection of SARS-CoV-2 with CRISPR-Cas12a

(2020) Dan Xiong et al.   PLOS BIOLOGY

Digital CRISPR/Cas‐Assisted Assay for Rapid and Sensitive Detection of SARS‐CoV‐2

(2020) Joon Soo Park et al.   Advanced Science

Evaluation of the usability of various rapid antibody tests in the diagnostic application for COVID-19

(2020) Yoshifumi Uwamino et al.   ANNALS OF CLINICAL BIOCHEMISTRY

Colorimetric Detection of SARS-CoV-2 and Drug-Resistant pH1N1 Using CRISPR/dCas9

(2020) Jeong Moon et al.   ACS Sensors

Commercialized diagnostic technologies to combat SARS-CoV2: Advantages and disadvantages

(2020) Briza Pérez-López et al.   TALANTA

CRISPR/Cas Systems towards Next-Generation Biosensing

(2019) Yi Li et al.   TRENDS IN BIOTECHNOLOGY

CRISPR-Cas based targeting of host and viral genes as an antiviral strategy

(2019) Lulia Koujah et al.   SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

Reduced False Positives and Improved Reporting of Loop-Mediated Isothermal Amplification using Quenched Fluorescent Primers

(2019) Patrick Hardinge et al.   Scientific Reports

SHERLOCK: nucleic acid detection with CRISPR nucleases

(2019) Max J. Kellner et al.   Nature Protocols

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

(2019) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

CRISPR-Cas3 induces broad and unidirectional genome editing in human cells

(2019) Hiroyuki Morisaka et al.   Nature Communications

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

Comparative genomics and evolution of trans-activating RNAs in Class 2 CRISPR-Cas systems

(2018) Guilhem Faure et al.   RNA Biology

Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang

(2018) Su Bin Moon et al.   Nature Communications

Programmable RNA recognition and cleavage by CRISPR/Cas9

(2014) Mitchell R. O’Connell et al.   NATURE

Live virus-free or die: coupling of antivirus immunity and programmed suicide or dormancy in prokaryotes

(2012) Kira S Makarova et al.   Biology Direct

Ambient temperature detection of PCR amplicons with a novel sequence-specific nucleic acid lateral flow biosensor

(2012) Geik Yong Ang et al.   BIOSENSORS & BIOELECTRONICS

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE