Article
Engineering, Biomedical
Tingting Fan, Shuo Wang, Zongmin Jiang, Shen Ji, Wenhua Cao, Wenli Liu, Yun Ji, Yujing Li, Ng Shyh-Chang, Qi Gu
Summary: 3D printing is an effective method for recreating skeletal muscle tissue, but obtaining large volumes of highly aligned muscle cells remains a challenge. This study demonstrates a feasible strategy for engineering skeletal muscle tissue using sequential culture processes and investigates the role of physical factors in muscle fiber alignment and maturity.
Article
Engineering, Biomedical
Jacqueline Bliley, Joshua Tashman, Maria Stang, Brian Coffin, Daniel Shiwarski, Andrew Lee, Thomas Hinton, Adam Feinberg
Summary: This study demonstrates the 3D bioprinting of a simplified heart model resembling embryonic development. The engineered heart tubes were cellularized and showed synchronous contractions similar to natural heart beats. These findings establish the feasibility of creating 3D bioprinted human heart tubes and open the door for engineering more complex heart muscle structures.
Article
Engineering, Biomedical
Zheng Zhong, Jing Wang, Jing Tian, Xiaoqian Deng, Alis Balayan, Yazhi Sun, Yi Xiang, Jiaao Guan, Jacob Schimelman, Henry Hwang, Shangting You, Xiaokang Wu, Chao Ma, Xiaoao Shi, Emmie Yao, Sophie X. Deng, Shaochen Chen
Summary: In this study, a 3D multicellular in vitro pterygium model was developed using a digital light processing (DLP)-based 3D bioprinting platform. The model consisted of human conjunctival stem cells (hCjSCs), immune cells, and vascular cells, and showed distinct gene expression patterns related to inflammation, angiogenesis, and epithelial mesenchymal transition. The model was validated with patient-derived pterygium tissues, demonstrating its potential for personalized medicine and drug screening.
Article
Engineering, Biomedical
Jesus M. Rodriguez-Rego, Laura Mendoza-Cerezo, Antonio Macias-Garcia, Alfonso C. Marcos-Romero, Juan P. Carrasco-Amador
Summary: Additive manufacturing technologies have many medical applications, mainly due to advancements in the development of materials used for customized models. However, obtaining correct cell viability values remains a challenge, which is why three-dimensional bioprinting is a valuable tool that requires extensive study. In this work, the comparison of 3D bioprinting technology using hydrogels and thermoplastics for biomimetic parts development is proposed. The goal is to study the printability of different materials and determine their ability to produce biomimetic structures with structural guarantees. Gelatin methacryloyl (GelMA) 5% was found to be the only material capable of generating biomimetic structures faithful to the designed 3D model. Additionally, it is shown that further development of hydrogels is needed to match the results achieved by thermoplastic materials in bioprinting.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Nanoscience & Nanotechnology
Carmen Radeke, Raphael Pons, Marko Mihajlovic, Jonas R. Knudsen, Sarkhan Butdayev, Paul J. Kempen, Charis-Patricia Segeritz, Thomas L. Andresen, Christian K. Pehmoller, Thomas E. Jensen, Johan U. Lind
Summary: In order to achieve the automated fabrication of complex tissue mimicking constructs through 3D bioprinting, it is necessary to develop bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties. Here, we present a scalable technique for generating nanofiber 3D printing inks with unique tissue-guiding capabilities. By tailoring the size and dispersibility of cellulose fibrils, we are able to create nanofibers that match the size and dimensions of natural collagen fibers, allowing for the orientation of cells and the spatial organization of engineered tissues during printing.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Biotechnology & Applied Microbiology
Gabriela S. Kronemberger, Guilherme A. S. C. Miranda, Renata S. N. Tavares, Bianca Montenegro, Ursula de A. Kopke, Leandra S. Baptista
Summary: Cancer is a major global disease with advancements in 3D cell culture technology enabling the replication of tumorigenesis and the establishment of solid tumor models, aiding in personalized medicine and drug discovery. Spheroids and organoids are explored as suitable 3D models for recreating tumorigenesis, while 3D bioprinting offers complex tissue models with challenges that need addressing for improved outcomes in cancer research.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
E. Fornetti, F. De Paolis, C. Fuoco, S. Bernardini, S. M. Giannitelli, A. Rainer, D. Seliktar, F. Magdinier, J. Baldi, R. Biagini, S. Cannata, S. Testa, C. Gargioli
Summary: Three-dimensional (3D) bioprinting has emerged as an optimal tool for tissue engineering. The extrusion-based approach is the most suitable for skeletal muscle tissue engineering due to its ability to produce parallel printing fibers that mimic native tissue architecture. The development of a novel extrusion-based 3D bioprinting system using PEG-Fibrinogen has shown promising results for muscle tissue regeneration, both in vitro and in vivo.
Article
Biochemistry & Molecular Biology
Borja Sanz, Ane Albillos Sanchez, Bonnie Tangey, Kerry Gilmore, Zhilian Yue, Xiao Liu, Gordon Wallace
Summary: Marine collagen, as an alternative source, shows potential in tissue engineering. The study revealed that marine collagen could serve as a low-cost, customizable, scalable, and quick-to-print platform for drug screening and researching neuromuscular junction physiology and pathogenesis.
Article
Engineering, Biomedical
Rafael Mestre, Nerea Garcia, Tania Patino, Maria Guix, Judith Fuentes, Mauricio Valerio-Santiago, Nuria Alminana, Samuel Sanchez
Summary: This study introduces a 3D-printed bioengineered human skeletal muscle platform, which efficiently models the three-dimensional structure of native tissue and plays a significant role in evaluating tissue differentiation, functionality, and force measurement. By assessing differentiation, maturation, force generation, contraction profiles, and changes in aging conditions, the flexibility of this platform for complex tissue modeling is validated.
Article
Immunology
Catherine M. Jankowski, Melissa P. Wilson, Samantha MaWhinney, Jane Reusch, Leslie Knaub, Sara Hull, Kristine M. Erlandson
Summary: The study found that individuals with HIV had blunted mitochondrial adaptations to exercise compared to uninfected controls, suggesting the need for different types of exercise-induced stimulation to improve mitochondrial function in this population.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Review
Geriatrics & Gerontology
Hawley E. Kunz, Ian R. Lanza
Summary: Aging is associated with various changes in skeletal muscle, including muscle mass loss, local inflammation, and reduced response to exercise. The chronic inflammation associated with aging, known as inflammaging, may contribute to these age-related muscle impairments. Inflammation and exercise have a complex relationship, as exercise can induce acute inflammation necessary for muscle repair, while chronic inflammation in aging may hinder this response. However, exercise training has anti-inflammatory effects and can target the underlying causes of inflammaging. This review explores the interplay between inflammation and exercise in aging and discusses potential therapeutic targets for enhancing exercise responsiveness in older adults.
EXPERIMENTAL GERONTOLOGY
(2023)
Article
Pharmacology & Pharmacy
Kyoungryong Kim, Subin Jin, Mikyung Shin
Summary: The use of fibrous hydrogel constructs is a promising strategy in tissue engineering due to their ability to mimic the structure of biological tissues. In this study, 3D-printed gelatin methacryloyl (GelMA) hydrogel microfibers were prepared and evaluated for their effect on myoblast differentiation. The presence of random microfibers in the 3D-printed constructs promoted myotubule-like cell formation and anisotropic myotubule formation.
ADVANCED THERAPEUTICS
(2023)
Review
Chemistry, Physical
Surendrasingh Y. Sonaye, Elif G. Ertugral, Chandrasekhar R. Kothapalli, Prabaha Sikder
Summary: Volumetric muscle loss (VML) is a severe acute skeletal muscle injury that can be treated using bioprinting methods. In this study, alginate-gelatin composite inks were developed as bioinks, and high-resolution scaffolds for VML treatment were successfully printed using extrusion printing. The optimal parameters for ink formulation and printing were determined, and the printed scaffolds showed potential for treating skeletal muscle defects.
Article
Biochemistry & Molecular Biology
Macsue Jacques, Shanie Landen, Javier Alvarez Romero, Danielle Hiam, Ralf B. Schittenhelm, Iresha Hanchapola, Anup D. Shah, Sarah Voisin, Nir Eynon
Summary: Exercise has significant effects on both the epigenome and proteome in human muscle. A 12-week high-intensity interval training intervention was conducted on 16 healthy moderately trained males, and muscle biopsies were collected at baseline and after 4, 8, and 12 weeks. The study found significant changes in proteins and DNA methylation levels, with some proteins consistently changed across individuals and the discovery of two novel exercise-related proteins. These findings contribute to a better understanding of the impact of exercise on the human body.
Article
Neurosciences
Ben Stocks, Stephen P. Ashcroft, Sophie Joanisse, Linda C. Dansereau, Yen Chin Koay, Yasir S. Elhassan, Gareth G. Lavery, Lake-Ee Quek, John F. O'Sullivan, Ashleigh M. Philp, Gareth A. Wallis, Andrew Philp
Summary: Acute supplementation of nicotinamide riboside (NR) does not affect substrate metabolism or NAD(+)-sensitive signaling pathways in human skeletal muscle during or after endurance exercise. Although NR supplementation does not increase skeletal muscle NAD(+) concentration, it does affect the concentration of NAD(+) metabolome, demonstrating skeletal muscle bioavailability of NR supplementation.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Cell Biology
Markus Rimann, Astrid Juengel, Sara Mousavi, Nicole Moeschlin, Maurizio Calcagni, Karin Wuertz-Kozak, Florian Brunner, Stefan Dudli, Oliver Distler, Christian Adlhart
Summary: This study demonstrated the suitability of two synthetic polymers in creating electrospun scaffolds for 3D skin cell models, with P(AN-MA) mats showing enhanced cell compatibility and infiltration compared to Pul/PVA/PAA mats.