Journal
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
Volume 20, Issue 1, Pages 213-219Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10942912.2016.1154571
Keywords
Osmotin-like protein; Sweet pepper; Eggplant; Liquid chromatography/tandem mass spectrometry; AQUA
Categories
Funding
- Ministry of Agriculture, Forestry, and Fisheries [NouEi 2-02]
Ask authors/readers for more resources
PR5-like protein and osmotin-like protein PR-5 are osmotin-like proteins found in sweet pepper and eggplant, respectively. These belong to the family of 5 pathogenesis-related proteins, which are considered a plentiful source of allergens. Recently, we established an assay to quantify protein NP24, an osmotin-like protein of tomato, using absolute quantification method with a stable isotope-labeled internal standard peptide (GQTWVINAPR[C-13(6),(15) N-4]) and liquid chromatography/tandem mass spectrometry. In this study, the peaks of GQTWVINAPR derived from PR5-like protein and osmotin-like protein PR-5 were detected by multiple reaction monitoring. The results indicated that the assay could be applied for the quantification of these proteins.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available