Review
Biochemistry & Molecular Biology
William Copp, Alexander Pontarelli, Christopher J. Wilds
Summary: Nucleic acids are ideal for constructing discrete structures and stimuli-responsive devices, with DNA tetrahedra being of particular interest for their easy uptake by cells and flexibility for modification. This minireview highlights recent advances in utilizing DNA tetrahedra for delivering therapeutic agents and sensing species within cells.
Review
Chemistry, Multidisciplinary
Bang Lin Li, Hang Zhang, Nian Bing Li, Hang Qian, David Tai Leong
Summary: This review provides a comprehensive materialistic background on the interaction of nucleic acids with non-nucleic acid-based materials. By combining the unique properties of nucleic acids with these materials, synergistic characteristics can be achieved, leading to attractive applications in bio-imaging, sensing, therapies, and information storage.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Yuxiang Dong, Jiliang Liu, Xuanzhao Lu, Jialin Duan, Liqi Zhou, Lizhi Dai, Min Ji, Ningning Ma, Yong Wang, Peng Wang, Jun-Jie Zhu, Qianhao Min, Oleg Gang, Ye Tian
Summary: Self-assembly processes are important for fabricating complexly organized nanomaterials, but often limited in creating structures with multiscale order. In this study, a two-stage assembly strategy is introduced to successfully form complexly organized nanoparticle crystals.
Article
Nanoscience & Nanotechnology
Sven Schnichels, Jose Hurst, Jan Willem de Vries, Sami Ullah, Katharina Froessl, Agnieszka Gruszka, Marina Loescher, Karl-Ulrich Bartz-Schmidt, Martin S. Spitzer, Andreas Herrmann
Summary: A drug-delivery system based on DNA nanoparticles loaded with brimonidine showed improved affinity towards the cornea, reduced intraocular pressure, and enhanced drug release compared to controls. The system demonstrated no toxicity and improved efficacy, potentially increasing patient compliance.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Shi Chen, Li Xing, Douglas Zhang, Alba Monferrer, Thomas Hermann
Summary: This study reported a method for designing and preparing nano-sandwich composite materials through noncovalent assembly of protein, DNA, and RNA modules, successfully synthesizing the three-dimensional structure of a protein-RNA-DNA complex and providing a new design approach for bottom-up preparation of layered biomaterials.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Shi Chen, Li Xing, Douglas Zhang, Alba Monferrer, Thomas Hermann
Summary: This study reports the successful synthesis of a composite material composed of protein, DNA, and RNA through controlled self-assembly and precise positioning, with the 3D architecture confirmed through fluorescence labeling and cryo-electron microscopy studies. The synthesis strategy for the nano-sandwich composite provides a general blueprint for controlled noncovalent assembly of complex supramolecular architectures.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Pharmacology & Pharmacy
Weina Ke, Kirill A. Afonin
Summary: Exosomes, as endogenous nanocarriers, show great promise in meeting the needs of nucleic acid nanoparticles in diagnostics and therapeutics. Their lipid membranes can carry different types of drugs and nucleic acids simultaneously and be surface modified, expanding their diverse biomedical applications.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Chemistry, Multidisciplinary
Yuhang Dong, Feng Li, Zhaoyue Lv, Shuai Li, Meihe Yuan, Nachuan Song, Jinqiao Liu, Dayong Yang
Summary: Coupling materials chemistry systems with biological processes to modulate lysosomal functions provides a promising approach for lysosome interference. This study demonstrates a new way to achieve lysosome interference by coupling subcellular microenvironment with a precisely programmable assembly system.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Arlin Rodriguez, Dhanush Gandavadi, Johnsi Mathivanan, Tingjie Song, Bharath Raj Madhanagopal, Hannah Talbot, Jia Sheng, Xing Wang, Arun Richard Chandrasekaran
Summary: The programmable nature of DNA allows the construction of custom-designed static and dynamic nanostructures. However, the high concentrations of magnesium ions required for assembly restrict their applications. In this study, we investigate the assembly of DNA nanostructures in various ions and successfully assemble a majority of these structures in Ca2+, Ba2+, Na+, K+ and Li+. We also find that structures assembled in monovalent ions exhibit significantly higher nuclease resistance compared to those assembled in divalent ions.
Article
Chemistry, Multidisciplinary
Taoran Tian, Dexuan Xiao, Tao Zhang, Yanjing Li, Sirong Shi, Wenyu Zhong, Ping Gong, Zhan Liu, Qian Li, Yunfeng Lin
Summary: The tFNA nanostructure mimicking the natural reaction of honeybees to dangers achieves active targeting therapy, leading to enhanced selective cytotoxicity against tumor cells.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Alesya Fokina, Yulia Poletaeva, Svetlana Dukova, Kristina Klabenkova, Zinaida Radkova, Anastasia Bakulina, Timofei Zatsepin, Elena Ryabchikova, Dmitry Stetsenko
Summary: A template-assisted assembly approach to a C24 fullerene-like double-stranded DNA polyhedral shell is proposed. The assembly employed a supramolecular oligonucleotide dendrimer as a 3D template that was obtained via the hybridization of siRNA strands and a single-stranded DNA oligonucleotide joined to three- or four-way branched junctions. The article discusses the use of a photolabile protecting group to prevent premature auto-hybridization of the self-complementary oligonucleotides, as well as the incorporation of cleavable linkers to guide the post-assembly disconnection of the shell. The study also explores the modification of siRNA strands to enhance enzymatic stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Qitian Lin, Junye Wu, Lili Jiang, Dexian Kong, Chao Xing, Chunhua Lu
Summary: The study developed an electrochemical biosensor based on Mg2+-dependent DNAzyme for simultaneous detection of miRNA-21 and miRNA-141, showing high sensitivity and selectivity. The biosensor offers advantages such as simple preparation and enzyme-free signal amplification, making it suitable for various bioanalysis and clinical diagnostics.
Article
Chemistry, Multidisciplinary
Yao Yin, Wei Xie, Mengyi Xiong, Yingying Gao, Qin Liu, Da Han, Guoliang Ke, Xiao-Bing Zhang
Summary: Inspired by the naturally confined enzymes on fluid cell membranes, a fluidly confined CRISPR-based DNA reporter (FINDER) was developed on living cell membranes, which was successfully applied for rapid and sensitive cancer cell identification in clinical blood samples. This new phenomenon was then combined with multiple aptamer-based DNA logic gate for cell recognition, thus a FINDER system capable of accurate, rapid and sensitive cancer cell identification was constructed.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Engineering, Biomedical
Jinpeng Han, Yuchen Cui, Zi Gu, Dayong Yang
Summary: The study developed a nanocarrier system that can efficiently release drugs in cells while minimizing side effects, utilizing polyphenol-DNA nanocomplexes. Through programmable assembly and disassembly processes, this method achieves precise release of multiple genes and drugs in cells.
Article
Chemistry, Multidisciplinary
Xiaokang Zhang, Yuan Liu, Bin Wang, Shihua Zhou, Peijun Shi, Ben Cao, Yanfen Zheng, Qiang Zhang, Kirilov Kasabov Nikola
Summary: The rise of DNA nanotechnology is driving the development of molecular security devices and bringing about important changes in information security technology. However, developing a secure and reliable access control strategy remains a challenge.
Article
Chemistry, Analytical
Tingting Zhai, Chenyun Sun, Dekai Ye, Kai Xia, Xiaoguo Liu, Chunhai Fan, Qian Li, Jianlei Shen
Summary: In this study, an electrochemical method was developed to control the formation of DNA nanostructures, and a high-sensitivity DNA nanostructure-based electrochemical biosensor was fabricated.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Liang Chen, Shihua Luo, Zhilei Ge, Chunhai Fan, Yang Yang, Qian Li, Yuanqing Zhang
Summary: The research team established an unbiased enrichment method for CTCs using a DNAzyme-catalyzed strategy based on DNA framework. This method has higher enrichment efficiency and lower bias compared to traditional methods, providing a new solution for CTC-based cancer diagnostics.
Article
Chemistry, Multidisciplinary
Chao Zhang, Yifang Yuan, Kaijie Wu, Yue Wang, Shitai Zhu, Jiye Shi, Lihua Wang, Qian Li, Xiaolei Zuo, Chunhai Fan, Chao Chang, Jiang Li
Summary: Research on the interactions between terahertz waves and DNA structures show that terahertz waves can induce unwinding of DNA duplexes and generate diverse DNA assemblies with high yields. This method allows in situ assembly of DNA origami in cell lysate, providing new insights into the bioeffects of terahertz waves.
Review
Chemistry, Multidisciplinary
Yuhan Wei, Kaizhe Wang, Shihua Luo, Fan Li, Xiaolei Zuo, Chunhai Fan, Qian Li
Summary: This article mainly introduces the latest research progress of DNA hydrogels as artificial extracellular matrix. It focuses on the classification, design, and assembly methods of DNA hydrogels, as well as their applications in cell encapsulation, cell culture, and tissue engineering. Meanwhile, the challenges and prospects for the cellular applications of DNA hydrogels are also discussed.
Review
Radiology, Nuclear Medicine & Medical Imaging
Wenyu Song, Yangmeihui Song, Qian Li, Chunhai Fan, Xiaoli Lan, Dawei Jiang
Summary: This review highlights recent advances in aptamer-based nuclear imaging and compares aptamer tracers with other biogenic probes. The fundamental properties of aptamer-based radiotracers are discussed, along with potential directions to improve their imaging performance. Despite the translational obstacles, aptamers are expected to become a versatile tool for cancer nuclear imaging in the near future.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Article
Chemistry, Multidisciplinary
Jue Yin, Mo Xie, Junke Wang, Meirong Cui, Dan Zhu, Shao Su, Chunhai Fan, Jie Chao, Qian Li, Lianhui Wang
Summary: In this work, the authors report a simple and versatile one-pot strategy to fabricate DNA architectures with the assistance of spherical gold nanoparticles modified with thiolated oligonucleotide strands. The desired architectures are formed via the stochastic co-assembly of different modules.
Article
Pharmacology & Pharmacy
Jizhou Tan, Ting Liu, Wenzhe Fan, Jialiang Wei, Bowen Zhu, Yafang Liu, Lingwei Liu, Xiaokai Zhang, Songling Chen, Haibiao Lin, Yuanqing Zhang, Jiaping Li
Summary: Cryoablation and microwave ablation are two main local treatments for hepatocellular carcinoma. This study found that cryoablation induced higher tumoral PD-L1 expression and more T cell infiltration, but less infiltration of PD-L1highCD11b+ myeloid cells compared to microwave ablation in hepatocellular carcinoma. Furthermore, cryoablation showed better curative effect when combined with anti-PD-L1 antibody in mouse models.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Chemistry, Multidisciplinary
Miao Mao, Zhun Lin, Liang Chen, Zhengyu Zou, Jie Zhang, Quanhao Dou, Jiacheng Wu, Jinglin Chen, Minhao Wu, Li Niu, Chunhai Fan, Yuanqing Zhang
Summary: By constructing modular DNA-origami-based nanoarrays, we improved the binding affinity of surface proteins by matching the spatial distribution of target protein clusters. The nanoarrays significantly enhanced the binding affinity of target cells and synergistically recognized low-affinity antigen-specific cells. Furthermore, the DNA nanoarrays successfully verified their precise recognition ability and high-affinity RLIs in the clinical detection of circulating tumor cells.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Pharmacology & Pharmacy
Zhun Lin, Zhengyu Zou, Zhe Pu, Minhao Wu, Yuanqing Zhang
Summary: The COVID-19 pandemic has accelerated antiviral research, with microfluidic technologies playing a crucial role in SARS-CoV-2 diagnosis and drug development. This review highlights the structure of SARS-CoV-2 and the basics of microfluidic design. It discusses the application of microfluidic devices in SARS-CoV-2 diagnosis, emphasizing their contribution to the manufacturing of accurate, sensitive, low-cost, and user-friendly virus-detection devices. Additionally, it explores the use of organ-on-a-chip and lipid nanoparticles (LNPs) synthesizing chips in antiviral drug screening and mRNA vaccine preparation, showcasing the potential of microfluidic technologies in ongoing research efforts and future viral outbreaks.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Chemistry, Multidisciplinary
Ling Wan, Jianfeng Ma, Jiasheng Yi, Yan Dong, Renjie Niu, Yan Su, Qian Li, Dan Zhu, Jie Chao, Shao Su, Chunhai Fan, Lianhui Wang, Ying Wan
Summary: This article introduces a CRISPR-based electrochemical biosensor for the ultrasensitive detection of avian influenza virus. The biosensor has a wide dynamic range, low detection limit, and excellent selectivity, providing a potential sensing platform for simple, fast, sensitive, and on-site detection of infectious diseases.
CHEMICAL COMMUNICATIONS
(2022)
Article
Nanoscience & Nanotechnology
Lingyu Feng, Cong Li, Lihua Wang, Jiang Li, Xiaoguo Liu, Qian Li, Shihua Luo, Jianlei Shen
Summary: In this study, we developed a stellate gold nanostructure-based SERS substrate for quantitative detection of neurotransmitters. By embedding an internal reference material within the nanostructure, we improved the accuracy and reproducibility of quantitative detection. Our self-referenced SERS substrate holds great potential for label-free, quantitative biosensing applications.
ACS APPLIED BIO MATERIALS
(2022)
