Article
Environmental Sciences
Yuan Cui, Qianqian Xiao, Qiong Zhang, Yuetong Liu, Weidong Hao, Jianjun Jiang, Qinghe Meng, Xuetao Wei
Summary: PM2.5 poses a threat to public health even at low levels, with black carbon (BC) being a key component. However, the crosstalk mechanism between necroptosis and macrophage extracellular traps (METs) after BC exposure has not been studied. Our research found that exposure to BC can trigger necroptosis and inhibit the formation of METs in macrophages, leading to weakened innate immune function.
ENVIRONMENTAL RESEARCH
(2023)
Review
Microbiology
Maria Garcia-Bengoa, Marita Meurer, Ralph Goethe, Mahavir Singh, Rajko Reljic, Maren von Koeckritz-Blickwede
Summary: Mycobacterium tuberculosis (M.tb) infections are a significant cause of mortality, and their control is difficult due to the emergence of antibiotic-resistant strains. M.tb is able to persist in the body for years without causing clinical manifestations, and it evades the host-defense by manipulating host-signalling pathways. Both macrophages and neutrophils play important roles in the immune response against M.tb.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Fei Han, Hao Chen, Lin Chen, Chenchen Yuan, Qinhao Shen, Guotao Lu, Weiwei Chen, Weijuan Gong, Yanbing Ding, Aidong Gu, Lide Tao
Summary: The persistent activation of neutrophils and excessive neutrophil extracellular traps (NETs) formation are the main factors causing pancreatic tissue injury and systemic inflammatory response in acute pancreatitis (AP). Inhibiting the release of NETs can effectively prevent the worsening of AP. This study found that the pore-forming protein gasdermin D (GSDMD) is active in neutrophils of AP mice and patients and plays a crucial role in NETs formation. Inhibition of GSDMD was shown to block NETs formation, reduce pancreatic injury, systemic inflammatory reaction, and organ failure in AP mice. Therefore, neutrophil GSDMD is identified as a therapeutic target for improving the occurrence and development of AP.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Immunology
Heng Cao, Junyu Liang, Jing Liu, Ye He, Yini Ke, Yiduo Sun, Song Jiang, Jin Lin
Summary: In both systemic lupus nephritis patients and mice, combination therapy effectively suppressed caspase-1/GSDMD-mediated pyroptosis and reduced disease progression. The numbers of caspase-1/PI positive cells in peripheral blood were positively correlated with the systemic lupus erythematosus disease activity index (SLEDAI).
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Hongmin Liang, Bing Wang, Junwei Wang, Bo Ma, Wenlong Zhang
Summary: This study investigated the pro-inflammatory properties of Trueperella pyogenes (T. pyogenes) and its secreted cytolysin, pyolysin (PLO). The results showed that T. pyogenes-induced inflammation is associated with PLO-induced pyroptosis and IL-1β release. The findings shed light on the pathogenesis of T. pyogenes and its interaction with the host's immune system.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Ting Shang, Zhi-Sen Zhang, Xin-Tong Wang, Jing Chang, Meng-En Zhou, Ming Lyu, Shuang He, Jian Yang, Yan-Xu Chang, Yuefei Wang, Ming-Chun Li, Xiumei Gao, Yan Zhu, Yuxin Feng
Summary: The study demonstrates that XBJ can inhibit NETs formation by reversing the GSDMD-related pathway and protect against sepsis-induced lung injury.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Medical Laboratory Technology
Shuofei Yang, Yu Feng, Liang Chen, Zheyu Wang, Jiaquan Chen, Qihong Ni, Xiangjiang Guo, Lan Zhang, Guanhua Xue
Summary: Diabetic foot ulcer (DFU) is a common complication of diabetes, with significant morbidity and mortality. Excessive release of neutrophil extracellular traps (NETs) delays wound healing in diabetic patients. This study identified the NLRP3/caspase-1/GSDMD pathway as a key regulator of NET release in diabetic wound tissue, and demonstrated that inhibition of GSDMD with disulfiram or genetic deletion of Gsdmd can accelerate diabetic wound healing by suppressing NET formation.
TRANSLATIONAL RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Yang Zhou, Tong Du, Chun-Lin Yang, Tao Li, Xiao-Li Li, Wei Liu, Peng Zhang, Jing Dong, Wei-Yue Si, Rui-Sheng Duan, Cong-Cong Wang
Summary: This study shows that delivering caspase-1 inhibitors through extracellular vesicles (EVs) is an effective treatment for myasthenia gravis and holds promise for clinical applications.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Pharmacology & Pharmacy
Jiezhou Ye, Bo Zeng, Meiyan Zhong, Hongchun Li, Lihui Xu, Junxiang Shu, Yaofeng Wang, Fan Yang, Chunsu Zhong, Xunjia Ye, Xianhui He, Dongyun Ouyang
Summary: Scutellarin, a flavonoid from Erigeron breviscapus, acts as an inhibitor for caspase-11 activation in macrophages, reducing pyroptosis by inhibiting the release of caspase-11p26 and generation of GSDMD-NT. It also suppresses the activation of NLRP3 inflammasome and regulates the PKA signaling pathway.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Immunology
Chuyi Tan, Bridgette Reilly, Alok Jha, Atsushi Murao, Yongchan Lee, Max Brenner, Monowar Aziz, Ping Wang
Summary: This study suggests that eCIRP is actively released from viable macrophages through GSDMD pores. Inhibition of GSDMD can reduce the release of eCIRP and potentially inhibit eCIRP-mediated inflammation in sepsis.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Tianli Chen, Yue Wang, Zhaodi Nan, Jie Wu, Ailu Li, Tingguo Zhang, Xun Qu, Chen Li
Summary: METs are a potential biomarker for CC patient prognosis and there is a positive feedback loop between CC cells and METs, which can be inhibited by PAD2-IN-1 inhibitors in vitro and in vivo.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marco Buscetta, Marta Cristaldi, Maura Cimino, Agnese La Mensa, Paola Dino, Fabio Bucchieri, Francesca Rappa, Santina Amato, Tommaso Silvano Aronica, Elisabetta Pace, Alessandro Bertani, Chiara Cipollina
Summary: The mechanisms and consequences of GSDMD activation in cigarette smoke-associated inflammation and lung disease are not well understood. This study shows that cigarette smoke extract (CSE) can activate caspase-1 and induce GSDMD cleavage and increased cell permeability. The activation of caspase-4 and caspase-8 by CSE further promotes GSDMD cleavage. These findings suggest that ASC-independent activation of caspase-1, -4, and -8 and subsequent GSDMD cleavage may contribute to macrophage dysfunction and chronic inflammation in the lungs of smokers.
Article
Chemistry, Multidisciplinary
Qian Cheng, Jing Pan, Zhuan-li Zhou, Fan Yin, Hong-yan Xie, Pan-pan Chen, Jing-yao Li, Pei-qing Zheng, Li Zhou, Wei Zhang, Jun Liu, Li-min Lu
Summary: The study found that under hyperglycemic condition, caspase-11/4 and GSDMD-mediated pyroptosis are activated and involved in podocyte loss.
ACTA PHARMACOLOGICA SINICA
(2021)
Article
Reproductive Biology
Chuncheng Lu, Zhao Wu, Hongbin Gao, Haiyuan Li, Renbin Deng, Ning Luo, Shipeng Fan, Xi Li, Danpeng He, Hui Zhao
Summary: This study revealed that sperm can induce macrophages to produce extracellular traps, which is dependent on phagocytosis and NADPH oxidase. Sperm from asthenozoospermia donors are more likely to be phagocytosed by macrophages, leading to increased release of macrophage extracellular traps.
BIOLOGY OF REPRODUCTION
(2023)
Article
Environmental Sciences
Yuan Cui, Qianqian Xiao, Yuese Yuan, Yimeng Zhuang, Weidong Hao, Jianjun Jiang, Qinghe Meng, Xuetao Wei
Summary: The impacts of environmental PM 2.5 on public health are a global concern. Studies have shown that PM 2.5 poses a threat to public health even at low levels. Ozone-oxidized black carbon (oBC) is a key component of PM 2.5 and can cause necroptosis in macrophages, weakening innate immune function. This study explored the crosstalk mechanism between necroptosis and macrophages extracellular traps (METs) after oBC exposure, finding that inhibition of necroptosis restored the formation of METs.
ENVIRONMENTAL POLLUTION
(2023)