Journal
NANOMATERIALS
Volume 11, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/nano11102669
Keywords
restricted access media; nanofibers; microfibers; on-line extraction; biological samples; column-switching chromatography
Categories
Funding
- GACR [20-19297S]
- STARSS [CZ.02.1.01/0.0/0.0/15_003/0000465]
- ERDF [1134119]
- GAUK [SVV 260 548]
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The study introduced the application of a poly-e-caprolactone composite sorbent consisting of micro- and nanometer fibers for the online extraction of non-steroidal anti-inflammatory drugs from a biological matrix, showing successful development of an effective method.
Application of the poly-e-caprolactone composite sorbent consisting of the micro- and nanometer fibers for the on-line extraction of non-steroidal anti-inflammatory drugs from a biological matrix has been introduced. A 100 mu L human serum sample spiked with ketoprofen, naproxen, sodium diclofenac, and indomethacin was directly injected in the extraction cartridge filled with the poly-e-caprolactone composite sorbent. This cartridge was coupled with a chromatographic instrument via a six-port switching valve allowing the analyte extraction and separation within a single analytical run. The 1.5 min long extraction step isolated the analytes from the proteinaceous matrix was followed by their 13 min HPLC separation using Ascentis Express RP-Amide (100 x 4.6 mm, 5 mu m) column. The recovery of all analytes from human serum tested at three concentration levels ranged from 70.1% to 118.7%. The matrix calibrations were carried out in the range 50 to 20,000 ng mL(-1) with correlation coefficients exceeding 0.996. The detection limit was 15 ng mL(-1), and the limit of quantification corresponded to 50 ng mL(-1). The developed method was validated and successfully applied for the sodium diclofenac determination in real patient serum. Our study confirmed the ability of the poly-e-caprolactone composite sorbent to remove the proteins from the biological matrix, thus serving as an alternative to the application of restricted-access media.
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