☆ 4.6 Article

Ecological Interactions of Cyanobacteria and Heterotrophs Enhances the Robustness of Cyanobacterial Consortium for Carbon Sequestration

FRONTIERS IN MICROBIOLOGY (2022)

Journal

FRONTIERS IN MICROBIOLOGY

Volume 13, Issue -, Pages -

Publisher

FRONTIERS MEDIA SA

DOI: 10.3389/fmicb.2022.780346

Keywords

cyanobacterial consortium; soda lakes; ecological niche; metagenomics; protein; SIP

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Abstract

The study characterized a cyanobacterial consortium enriched from microbial mats in alkaline soda lakes in BC, Canada, grown successfully for 4 years under high alkalinity conditions. Using shotgun metagenomic sequencing and metaproteomics, the consortium's stability was determined, showing tight carbon transfer from cyanobacteria to heterotrophic populations.

Lack of robustness is a major barrier to foster a sustainable cyanobacterial biotechnology. Use of cyanobacterial consortium increases biodiversity, which provides functional redundancy and prevents invading species from disrupting the production ecosystem. Here we characterized a cyanobacterial consortium enriched from microbial mats of alkaline soda lakes in BC, Canada, at high pH and alkalinity. This consortium has been grown in open laboratory culture for 4 years without crashes. Using shotgun metagenomic sequencing, 29 heterotrophic metagenome-assembled-genomes (MAGs) were retrieved and were assigned to Bacteroidota, Alphaproteobacteria, Gammaproteobacteria, Verrucomicrobiota, Patescibacteria, Planctomycetota, and Archaea. In combination with metaproteomics, the overall stability of the consortium was determined under different cultivation conditions. Genome information from each heterotrophic population was investigated for six ecological niches created by cyanobacterial metabolism and one niche for phototrophy. Genome-resolved metaproteomics with stable isotope probing using C-13-bicarbonate (protein/SIP) showed tight coupling of carbon transfer from cyanobacteria to the heterotrophic populations, specially Wenzhouxiangella. The community structure was compared to a previously described consortium of a closely related cyanobacteria, which indicated that the results may be generalized. Productivity losses associated with heterotrophic metabolism were relatively small compared to other losses during photosynthesis.

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Bacterial Communities Along Environmental Gradients in Tropical Soda Lakes

Thierry A. Pellegrinetti, Simone R. Cotta, Hugo Sarmento, Juliana S. Costa, Endrews Delbaje, Celia R. Montes, Plinio B. Camargo, Laurent Barbiero, Ary T. Rezende-Filho, Marli F. Fiore

Summary: This study investigated the effects of different environmental conditions on bacterial communities and their functioning in six adjacent soda lakes in the Pantanal biome, Brazil. The results showed that the lakes could be classified into three types based on wet and dry season data, and the bacterial community composition and functioning were consistent with this classification. The study also found that microbial abundance and diversity increased during the dry period, indicating the importance of precipitation-evaporation balance in these systems. The microbiome dataset of this study can serve as a baseline for restoring impacted soda lakes. The study highlights the sensitivity of tropical soda lakes to climate change and the potential for drastic shifts in community diversity.

MICROBIAL ECOLOGY (2023)