Article
Biotechnology & Applied Microbiology
Vinicius Bonetti Franceschi, Gabriel Dickin Caldana, Amanda de Menezes Mayer, Gabriela Bettella Cybis, Carla Andretta Moreira Neves, Patricia Aline Grohs Ferrareze, Meriane Demoliner, Paula Rodrigues de Almeida, Juliana Schons Gularte, Alana Witt Hansen, Matheus Nunes Weber, Juliane Deise Fleck, Ricardo Ariel Zimerman, Livia Kmetzsch, Fernando Rosado Spilki, Claudia Elizabeth Thompson
Summary: This study tracked the molecular evolution and spread of SARS-CoV-2 in Esteio, Southern Brazil, highlighting mutations from May to October 2020, including common mutations in spike glycoprotein and an emergent mutation in spike Receptor Binding Domain. The study also found epidemiologically-related clusters, demonstrating multiple introductions of prevalent Brazilian lineages and their spread to other regions. The correlation of clinical, epidemiological, and genomic information is crucial for understanding viral evolution and informing policy making strategies to combat COVID-19.
Article
Microbiology
Vinicius Bonetti Franceschi, Gabriel Dickin Caldana, Christiano Perin, Alexandre Horn, Camila Peter, Gabriela Bettella Cybis, Patricia Aline Grohs Ferrareze, Liane Nanci Rotta, Flavio Adsuara Cadegiani, Ricardo Ariel Zimerman, Claudia Elizabeth Thompson
Summary: Almost one year after the onset of the COVID-19 pandemic, new viral lineages with enhanced transmissibility, immune evasion, and mortality were identified in the United Kingdom, South Africa, and Brazil. In the state of Rio Grande do Sul, Brazil, the previously predominant B.1.1.28 and B.1.1.33 lineages were rapidly replaced by P.1 and P.2, with the emergence of the novel P.1.2 sublineage. Genomic surveillance revealed potential multiple introductions of P.1-derived clades in the region, warranting further investigation into the clinical and epidemiological implications of these emerging variants.
Review
Medicine, General & Internal
Arnav Agarwal, Ya Gao, Luis Enrique Colunga Lozano, Saad Asif, Layla Bakaa, Maryam Ghadimi, John Basmaji, Aninditee Das, Mark Loeb, Gordon Guyatt
Summary: This article describes a planned systematic review and meta-analysis that aims to evaluate the impact of shorter versus longer durations of antibiotic therapy for patients with CAP.
Article
Medicine, General & Internal
Elie Mulhem, Andrew Oleszkowicz, David Lick
Summary: This study in Southeast Michigan found that older age, male gender, certain chronic diseases, and use of specific medications were associated with higher in-hospital mortality among COVID-19 patients. As experience in treating the disease increased, the in-hospital mortality rate decreased. Black patients were more likely to be hospitalized and receive mechanical ventilation, but less likely to die in the hospital compared to white patients.
Article
Microbiology
Xiang Liu, Ali Zaid, Joseph R. Freitas, Nigel A. McMillan, Suresh Mahalingam, Adam Taylor
Summary: The study confirmed the value of the RG-rescued SARS-CoV-2 viruses in reproducing infection patterns and pathology typical of severe COVID-19 in a mouse model, providing important tools for advancing research on SARS-CoV-2 and COVID-19 therapeutics.
Article
Virology
Ana B. Gorini da Veiga, Leticia G. Martins, Irina Riediger, Alix Mazetto, Maria do Carmo Debur, Tatiana S. Gregianini
Summary: Respiratory viral infections can be severe, especially for children, elderly, and patients with comorbidities. In Brazil, the official surveillance system investigates various viruses, but some cases with no identified pathogens remain. This study analyzed viruses in patients without underlying diseases, revealing the potential for coronaviruses to cause severe disease in healthy adults.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Nadia B. Olivero, Ana S. Gonzalez-Reiche, Viviana E. Re, Gonzalo M. Castro, Maria B. Pisano, Paola Sicilia, Maria G. Barbas, Zenab Khan, Adriana van de Guchte, Jayeeta Dutta, Paulo R. Cortes, Mirelys Hernandez-Morfa, Victoria E. Zappia, Lucia Ortiz, Ginger Geiger, Daniela Rajao, Daniel R. Perez, Harm van Bakel, Jose Echenique
Summary: This study describes the association of clinical characteristics with the clinical outcomes of survivors and non-survivors of COVID-19 patients, and the specific mutations found in the genome sequences of SARS-CoV-2 in each patient group. The results show the selective nature of SARS-CoV-2 mutations in different patient groups and provide new genomic data on the virus evolution.
Article
Multidisciplinary Sciences
Matthew D. Solomon, Gabriel J. Escobar, Yun Lu, David Schlessinger, Jonathan B. Steinman, Lawrence Steinman, Catherine Lee, Vincent X. Liu
Summary: Susceptibility and severity of COVID-19 infection vary widely. Prior exposure to endemic coronaviruses, common in young children, may protect against SARS-CoV-2. In adults, less exposure to young children is associated with more severe COVID-19 illness.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Godagama Gamaarachchige Dinesh Suminda, Srishti Bhandari, Yoonkyung Won, Umesh Goutam, Krishna Kanth Pulicherla, Young-Ok Son, Mrinmoy Ghosh
Summary: Globalization, agricultural intensification, urbanization, and climatic changes have led to an increase in emerging infectious zoonotic diseases. Innovative and effective research is needed to understand the transmission, ecological implications, and dynamics of these diseases at human-wildlife interfaces. High-throughput sequencing methodologies have the potential to improve detection and surveillance of zoonotic infections in livestock. This study investigates the current use of sequencing technologies and explores their potential for better infectious disease diagnosis and monitoring in farm animals.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Editorial Material
Geriatrics & Gerontology
Rodrigo Nunez-Cortes, Ruben Lopez-Bueno, Rodrigo Torres-Castro, Camilo Soto-Carmona, Maritza Ortega-Palavecinos, Sofia Perez-Alenda, Lilian Solis-Navarro, Oscar Diaz-Cambronero, Francisco M. Martinez-Arnau, Joaquin Calatayud
Summary: The aim of this prospective study was to examine the risk factors most associated with mortality one year after diagnosis of COVID-19. The study found that age was the most important risk factor, and disabilities, institutionalization, and socioeconomic status were also significantly associated with mortality.
Article
Medicine, General & Internal
Dacre R. T. Knight, Bala Munipalli, Ilana I. Logvinov, Meghana G. Halkar, Ghada Mitri, Abd Moain Abu Dabrh, Stephanie L. Hines
Summary: This study aims to evaluate, characterize, and describe the prevalence and predicting factors of severe COVID-19 infection and post-acute sequelae of COVID-19 (PASC) reported by patients. The study found that over one-third of patients had PASC, and persistent symptoms were associated with disease severity, gender, psychological symptoms, and inability to resume normal activities.
AMERICAN JOURNAL OF THE MEDICAL SCIENCES
(2022)
Article
Medicine, General & Internal
Zubair Akhtar, Md Ariful Islam, Mohammad Abdul Aleem, Syeda Mah-E-Muneer, M. Kaousar Ahmmed, Probir K. Ghosh, Mustafizur Rahman, Mohammed Ziaur Rahman, Mariya Kibtiya Sumiya, Md Mahfuzur Rahman, Tahmina Shirin, A. S. M. Alamgir, Sayera Banu, Mahmudur Rahman, Fahmida Chowdhury
Summary: The study aimed to estimate the proportion of SARS-CoV-2 and influenza virus coinfection among severe acute respiratory infection (SARI) cases-patients in Bangladesh. The results showed that coinfection with SARS-CoV-2 and influenza virus was not very common and had less disease severity in terms of mortality in Bangladesh during the first wave of COVID-19 pandemic. Additionally, there was no circulating influenza virus during the usual peak season in 2020, indicating a unique phenomenon during the pandemic.
Article
Medicine, General & Internal
George Paasi, Charles Benard Okalebo, Paul Ongodia, Cate Namayanja, Egiru Emma Isaiah Eregu, Grace Abongo, Moses Olupot, Denis Amorut, Rita Muhindo, William Okiror, Carolyne Ndila, Peter Olupot-Olupot, PARIST Trial Study Grp
Summary: This study aims to assess the feasibility, safety, and effectiveness of paracetamol in children with haemoglobinuric severe malaria. It is a randomized controlled trial involving 40 children, with the primary outcome being renal recovery at 48 hours. The results will provide preliminary data for the treatment of acute kidney injury in paediatric malaria.
Article
Medicine, General & Internal
Heather F. Gidding, Dorothy A. Machalek, Alexandra J. Hendry, Helen E. Quinn, Kaitlyn Vette, Frank H. Beard, Hannah S. Shilling, Rena Hirani, Iain B. Gosbell, David O. Irving, Linda Hueston, Marnie Downes, John B. Carlin, Matthew V. N. O'Sullivan, Dominic E. Dwyer, John M. Kaldor, Kristine Macartney
Summary: The study estimated SARS-CoV-2-specific antibody seroprevalence in Sydney after the first epidemic wave of COVID-19, with results showing a prevalence below 1%, indicating low community transmission. Early control measures were successful in limiting the spread of COVID-19, but ongoing efforts to reduce transmission are still crucial.
MEDICAL JOURNAL OF AUSTRALIA
(2021)
Article
Public, Environmental & Occupational Health
Hong-Miao Li, Fei Tang, Li-Jun Wang, Qian Huang, Hai-Feng Pan, Tian-Ping Zhang
Summary: This study analyzed the association of m6A readers' genes variation and expression levels with pulmonary tuberculosis (PTB). The results showed that SNPs in these genes did not contribute to PTB susceptibility, but their decreased expression levels in PTB patients suggested their significant roles in PTB.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Biotechnology & Applied Microbiology
Vinicius Bonetti Franceschi, Gabriel Dickin Caldana, Amanda de Menezes Mayer, Gabriela Bettella Cybis, Carla Andretta Moreira Neves, Patricia Aline Grohs Ferrareze, Meriane Demoliner, Paula Rodrigues de Almeida, Juliana Schons Gularte, Alana Witt Hansen, Matheus Nunes Weber, Juliane Deise Fleck, Ricardo Ariel Zimerman, Livia Kmetzsch, Fernando Rosado Spilki, Claudia Elizabeth Thompson
Summary: This study tracked the molecular evolution and spread of SARS-CoV-2 in Esteio, Southern Brazil, highlighting mutations from May to October 2020, including common mutations in spike glycoprotein and an emergent mutation in spike Receptor Binding Domain. The study also found epidemiologically-related clusters, demonstrating multiple introductions of prevalent Brazilian lineages and their spread to other regions. The correlation of clinical, epidemiological, and genomic information is crucial for understanding viral evolution and informing policy making strategies to combat COVID-19.
Article
Medicine, General & Internal
Ricardo Ariel Zimerman, Patricia Aline Groehs Ferrareze, Flavio Adsuara Cadegiani, Carlos Gustavo Wambier, Daniel do Nascimento Fonseca, Andrea Roberto de Souza, Andy Goren, Liane Nanci Rotta, Zhihua Ren, Claudia Elizabeth Thompson
Summary: P.1 (Gamma) lineage, a variant of concern of SARS-CoV-2, was first identified in the State of Amazonas, Brazil, and has shown continuous evolution with rare mutations in spike and non-structural proteins. The lineage is associated with increased infectivity, higher mortality, and immune evasion, potentially impacting vaccine efficacy. The emergence of P.1 is mainly driven by strong evolutionary forces and the accumulation of favorable mutations.
FRONTIERS IN MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Patricia Aline Grohs Ferrareze, Ricardo Ariel Zimerman, Vinicius Bonetti Franceschi, Gabriel Dickin Caldana, Paulo Augusto Netz, Claudia Elizabeth Thompson
Summary: The COVID-19 pandemic caused by SARS-CoV-2 has resulted in over 380 million cases and 5.5 million deaths worldwide as of February 2022. The spike protein plays a crucial role in the interaction between the virus and host cells, binding to the hACE2 receptor and facilitating membrane fusion. This protein is a major target for neutralizing antibodies against SARS-CoV-2. The presence of multiple amino acid substitutions, particularly in the receptor binding domain (RBD), has increased the binding affinity to hACE2 and led to changes in the pathogenesis of SARS-CoV-2, potentially impacting vaccine development. This study identified 48 sites under selective pressures, including mutations at sites 138, 142, 222, 262, 484, 681, and 845 that showed strong evidence of selective pressure. Coevolutionary analysis revealed that certain sites, such as E484K-N501Y, were not conditionally independent. Molecular dynamics simulations and free energy estimates demonstrated the stabilizing effect of the E484K mutation on the spike RBD-hACE2 binding affinity in the P.1 and P.2 lineages, particularly when combined with the L452V mutation. Structural changes were also observed in the hACE molecule when interacting with the B.1.1.7 RBD. Despite some destabilizing substitutions, most of the positively selected mutations had a stabilizing effect.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Isabelle A. L. Postiga, Paula C. Teixeira, Carla Andretta Moreira Neves, Paulo Santana Filho, Bruna Marmett, Roseana Carvalho, Alessandra Peres, Liane Rotta, Claudia Elizabeth Thompson, Gilson P. Dorneles, Pedro R. T. Romao
Summary: The aim of this study was to evaluate the systemic redox state and inflammatory markers in ICU or non-ICU severe COVID-19 patients during the hospitalization period. The results showed that ICU patients had increased levels of C-reactive protein, reactive oxygen species (ROS), and protein oxidation over the hospitalization period compared to non-ICU patients, despite increased antioxidant status. Granulocytes and monocytes of COVID-19 patients had lower mitochondrial membrane potential (MMP) and higher ROS production compared to healthy controls, with ICU patients showing the highest values. Furthermore, incubation of THP-1 cells with plasma from ICU COVID-19 patients during the hospitalization period decreased cell viability and increased apoptosis rate.
CELL BIOCHEMISTRY AND FUNCTION
(2022)
Article
Urology & Nephrology
Viviane Schmitt Jahnke, Jose AntonioTesser Poloni, Carla Andretta Moreira Neves, Camila Peter, Claudia Elizabeth Thompson, Liane Nanci Rotta
Summary: This article describes a case of acute kidney injury associated with rhabdomyolysis in a patient with COVID-19. Patients with this condition may be asymptomatic or experience symptoms such as muscle pain, dark urine, significant electrolyte imbalance, and acute kidney injury.
JORNAL BRASILEIRO DE NEFROLOGIA
(2022)
Article
Biochemistry & Molecular Biology
Claudia Elizabeth Thompson, Lauis Brisolara-Correa, Helen Nathalia Thompson, Hubert Stassen, Loreta Brandao de Freitas
Summary: Plant RNases T2 play important roles in various physiological and developmental processes. Differences in amino acid flexibility, particularly in substrate-binding regions, between S-like RNases and S-RNases are crucial for understanding the RNA-binding process. The positively selected amino acids located in flexible regions of these RNases may be involved in interactions with pathogen proteins and viral RNA. Selective pressures have led to increased amino acid variability and structural differences within and among S-like RNases and S-RNases, which are essential for their functional diversity.
GENETICS AND MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Lucas C. Beltrame, Claudia E. Thompson, Loreta B. Freitas
Summary: The association between plants and their pollinators plays a crucial role in increasing angiosperm diversity. This study examined two proteins, CFAT and BPBT, involved in the production of volatile organic compounds in plants, focusing on their evolutionary, structural, and functional aspects in Solanaceae. Bayesian inference and comparative molecular modeling techniques were used to analyze the proteins' evolutionary history and obtain 3D models. The phylogenetic tree revealed diverse enzymes with transferase domains in Solanaceae, and the amino acid residues essential for enzymatic activity and substrate interaction were identified.
GENETICS AND MOLECULAR BIOLOGY
(2023)
Article
Virology
Amanda de Menezes Mayer, Patricia Aline Grohs Ferrareze, Luiz Felipe Valter de Oliveira, Tatiana Schaffer Gregianini, Carla Lucia Andretta Moreira Neves, Gabriel Dickin Caldana, Livia Kmetzsch, Claudia Elizabeth Thompson
Summary: This study analyzed the genomes of SARS-CoV-2 from Esteio city in Rio Grande do Sul, Brazil, and compared them with genomes from the first two years of the pandemic in the state. The analysis revealed phylogenetically distinct groups for different lineages, including Alpha, Gamma, Delta, and Omicron. Molecular evolution analysis identified specific sites under adaptive selection in membrane and nucleocapsid proteins, indicating potential effects on protein structure stability.
Article
Virology
Xavier Didelot, Vinicius Franceschi, Simon D. W. Frost, Ann Dennis, Erik M. Volz
Summary: Inference of effective population size from genomic data can provide insights into demographic history and epidemiological dynamics. A nonparametric approach based on latent process models is developed to estimate the population size dynamics, optimizing parameters using out-of-sample prediction accuracy. The methodology is demonstrated using simulation experiments and applied to HIV-1 and SARS-CoV-2 datasets to estimate the impact of interventions on epidemic dynamics.
Article
Paleontology
Mario G. F. Esperanca Junior, Gabriela B. Cybis, Roberto Iannuzzi
Summary: Glossopteris leaves, the most abundant floristic element in the Gondwanan continent, have conservative morphology that has made them difficult to use for environmental reconstruction and climate change identification. This paper proposes a new rapid method to estimate the venation density of Glossopteris leaves, which has shown significant differentiation between leaves produced in wet and drier ecosystems. This study suggests that environmental stress controlled the vein architecture of Palaeozoic plants, similar to plants in modern ecosystems.
Proceedings Paper
Computer Science, Information Systems
Gustavo Henrique Cervi, Cecilia Dias Flores, Claudia Elizabeth Thompson
Summary: Clinical metagenomics is a technique for searching for infectious agents in biological samples. The large volume of data generated by DNA sequencers requires specialized computational methods to accelerate the search and ensure accurate results.
PROCEEDINGS OF SIXTH INTERNATIONAL CONGRESS ON INFORMATION AND COMMUNICATION TECHNOLOGY (ICICT 2021), VOL 2
(2022)
Article
Genetics & Heredity
Desiree Deconte, Catarine Benta Lopes dos Santos, Camila Ohomoto de Morais, Tatiane Mayumi Yonamine, Leticia Thais Nogueira, Maria Angelica Tosi Ferreira, Vinicius Bonetti Franceschi, Andre Luis Soares Longhi, Rolando Andre Rios Villacis, Silvia Regina Rogatto, Rodrigo Ligabue-Braun, Paulo Ricardo Gazzola Zen, Rafael Fabiano Machado Rosa, Marilu Fiegenbaum
Summary: Marshall-Smith syndrome (MSS) is a rare genetic condition with a variety of clinical features, some of which may be underestimated. This study describes a patient with unusual clinical manifestations and a novel NFIX variant not previously reported in the literature. The findings suggest potential mechanisms for impaired NFIX protein function in MSS.
Article
Virology
Mohamed S. H. Hassan, Ahmed Ali, Motamed Elsayed Mahmoud, Danah Altakrouni, Shahnas M. Najimudeen, Mohamed Faizal Abdul-Careem
Summary: This study compared the efficacy of different vaccination programs against Canadian DMV/1639 IBV and found that the autogenous inactivated DMV/1639 vaccine was more effective in reducing viral loads and boosting virus neutralization titers. Both vaccination programs provided significant protection against pathological effects.
Article
Virology
Wanda Christ, Jonas Klingstrom, Janne Tynell
Summary: The integrated stress response (ISR) is activated in response to SARS-CoV-2 infection, triggering translational arrest and inhibiting stress granule formation. However, the stress-responsive transcription factors ATF4 and CHOP are not induced. Different SARS-CoV-2 variants show variant-specific differences in ISR activation, suggesting potential impacts on pathogenesis and treatment strategies.
Article
Virology
Xiao Han, Hankun Xu, Yifan Weng, Rong Chen, Jidong Xu, Tong Cao, Renjie Sun, Ying Shan, Fang He, Weihuan Fang, Xiaoliang Li
Summary: Classical swine fever virus (CSFV) can inhibit the host innate immunity by decreasing the level of high mobility group box 1 (HMGB1), and CSFV Npro can evade host antiviral immune response through upregulating HMGB1 acetylation.
Article
Virology
Sunjian Lyu, Fulei Xiong, Tianpeng Qi, Weifeng Shen, Qi Guo, Mingming Han, Li Liu, Weishao Bu, Julin Yuan, Bao Lou
Summary: This study identified and characterized a novel temperate A. hydrophila phage, P05B, which has strong lytic ability and stability across different temperatures and pH values.
Article
Virology
Siyuan Liu, Wei Chen, Raphael Nyaruaba, Shunlong Wang, Cihan Yang, Qun Wu, Ying Liu, Puyu Liu, Fei Wang, Jingling Wang, Zhiming Yuan, Dingwei Sun, Han Xia
Summary: Specific, sensitive, and reliable RT-qPCR assays for the detection of OYAV and EBIV have been successfully developed in this study. These assays hold great promise for their potential application in clinical and field samples in the future.
Article
Virology
Rui Wang, Qiang Sun, Jinbo Xiao, Congcong Wang, Xiaoliang Li, Jichen Li, Yang Song, Huanhuan Lu, Ying Liu, Shuangli Zhu, Zhijun Liu, Yong Zhang
Summary: Coxsackievirus A6 (CVA6) has emerged as an important agent causing hand, foot, and mouth disease (HFMD). Mutations in residue 64 of the virus have been found to increase resistance to ribavirin and decrease sensitivity to guanidine hydrochloride. This mutation also leads to increased replication fidelity and reduced pathogenicity. These findings provide a foundation for the development of a safe and effective live attenuated CVA6 vaccine.
Article
Virology
Yao Xiong, Keran Ma, Xiao Zou, Yantao Liang, Kaiyang Zheng, Tiancong Wang, Hong Zhang, Yue Dong, Ziyue Wang, Yundan Liu, Hongbing Shao, Andrew Mcminn, Min Wang
Summary: This study isolated a novel virus, vB_VviC_ZQ26, infecting Vibrio cyclitrophicus from coastal waters near Qingdao, China. It was found that vB_VviC_ZQ26 belongs to a new vibriophage-specific family, Coheviridae, and is mainly found in temperate and tropical epipelagic zones.
Article
Virology
Xing Li, Zhiping Ye, Ewan P. Plant
Summary: By analyzing clinical and cultured samples using the same bioinformatic pipeline, it was found that 5' copyback DVGs are prevalent in human clinical samples but not in cultured samples. Furthermore, there are differences in DVG production and composition between in vivo and in vitro infections.
Article
Virology
Shreya Banerjee, Rakesh Sarkar, Arpita Mukherjee, Suvrotoa Mitra, Animesh Gope, Mamta Chawla-Sarkar
Summary: Rotavirus is a major cause of viral gastroenteritis in infants and continues to be a severe issue in underdeveloped nations. Recent research has shown that a long non-coding RNA called SLC7A11-AS1 is upregulated during rotavirus infection and plays a role in facilitating the virus's propagation by regulating intracellular glutathione and lipid peroxidation.
Article
Virology
Eiichi Hondo, Tetsufumi Katta, Ayato Sato, Naoya Kadofusa, Tomoki Ishibashi, Hiroshi Shimoda, Hirokazu Katoh, Atsuo Iida
Summary: Bat-borne emerging viruses, such as the Ebola virus and Nipah virus, pose a significant threat to public health. In this study, we identified micafungin as a potential antiviral drug against Pteropine orthoreovirus (PRV) and found that it inhibits viral release in human cells through activating the endoplasmic reticulum stress response pathway. This research provides valuable insights into the development of effective treatments for future virus pandemics.
Article
Virology
M. Joubert, N. van den Berg, J. Theron, V. Swart
Summary: This study investigated the host gene expression in asymptomatic avocado nursery trees infected with Avocado sunblotch viroid (ASBVd) using RNA sequencing. The results showed that 631 genes were differentially expressed during infection, with 63% upregulated. ASBVd infection significantly affected plant defence responses, phytohormone networks, gene expression pathways, secondary metabolism, cellular transport, as well as protein modification and degradation. This study provides insights into the molecular interactions underlying avsunviroid-host interactions.
Article
Virology
Wanling Zhang, Haiyan Yang, Zhengyun Liu, Shengyu Wang, Tianyang Chen, Hong Song, Yunbin Xu, Fajin Li, Guo Luo, Huan Wang
Summary: EV71 infection leads to changes in mitochondrial morphology and dynamics in human neuroblastoma cells, resulting in mitochondrial dysfunction that may contribute to nervous system dysfunction.
Article
Virology
Mikhail Oliveira Leastro, Elliot Watanabe Kitajima, Vicente Pallas, Jesus Angel Sanchez- Navarro
Summary: This study reports the construction and validation of an infectious cDNA clone of Citrus leprosis virus C (CiLV-C) based on an agroinfection system. Agro-injected Nicotiana benthamiana plants showed localized lesions similar to the natural infection process. The virus recovered from the infected plant tissue could be mechanically transmitted between plants. The CiLV-C reverse genetic system provides a powerful molecular tool for unraveling the peculiarities of the citrus leprosis pathosystem.
Article
Virology
Manel Essaidi-Laziosi, Francisco J. Perez-Rodriguez, Catia Alvarez, Pascale Sattonnet-Roche, Giulia Torriani, Meriem Bekliz, Kenneth Adea, Matthias Lenk, Tasnim Suliman, Wolfgang Preiser, Marcel A. Mueller, Christian Drosten, Laurent Kaiser, Isabella Eckerle
Summary: This study found that the Omicron variant has different replication characteristics compared to other variants of concern, being able to efficiently produce infectious virus in nasal cells but not lung cells. In addition, the Delta and Omicron variants showed increased infection efficiency in A549 cells. These findings suggest that the variants of concern have better adaptation to humans but do not have an extended host range.
Article
Virology
Martin Faye, Modeste Name Faye, Babacar Ndiaye, Moussa Moise Diagne, Safietou Sankhe, Ndeye Marieme Top, Amadou Diallo, Cheikh Loucoubar, Ndongo Dia, Amadou Alpha Sall, Ousmane Faye
Summary: Genomic surveillance in Senegal since March 2020 has detected the emergence of Omicron BA.4 and BA.5 sublineages in June 2022. Next-generation sequencing and phylogeny analysis of isolates from a cluster of cases in Northern Senegal in July 2022 revealed that the BA.4 cases originated from a XAS recombinant, marking the first reported sequence of this variant from Senegal. Continuous genomic surveillance of positive SARS-CoV-2 samples is crucial.
