Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 262, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2021.120087
Keywords
Alkaline phosphatase; Ratiometric fluorescent sensor; Gold nanoclusters; Quantum dots; Cell imaging
Categories
Funding
- National Natural Science Foundation of China [22004046, 22074052]
- Science and Technology Developing Foundation of Jilin Province [20200602047ZP]
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A ratiometric fluorescent sensor based on MoS2 quantum dots and AuNCs was developed for determination and imaging of alkaline phosphatase. The sensor exhibited a linear response to ALP concentration with a low detection limit and was successfully employed in bioimaging of intracellular ALP in living cells.
Herein, a ratiometric fluorescent sensor based on MoS2 quantum dots (QDs) and glutathione-capped gold nanoclusters (AuNCs) was developed for determination and imaging of alkaline phosphatase (ALP). The sensor was developed by covalently linking QDs with AuNCs to form stable MoS2@AuNCs nanohybrids that exhibited the blue fluorescence of MoS2 QDs and the red fluorescence of AuNCs. In the presence of Ce3+, the fluorescence intensity of AuNCs was increased due to the aggregation-induced emission enhancement (AIEE), while that of MoS2 QDs remained unchanged, thus could be used as a reference signal. After adenosine 5'-monophosphate (AMP) and ALP were introduced into the system, AMP was hydrolyzed to adenosine and phosphate ions (PO43-). Owing to higher affinity between Ce3+ and PO43-, the AIEE effect was inhibited, in turn resulting in the decrease of AuNCs fluorescence. The developed ratiometric fluorescent sensor had a linear response to ALP concentration ranging from 0.5 to 50 U L-1 with a detection limit (LOD) of 0.08 U L-1. Moreover, the sensor had low cytotoxicity and was successfully employed in lysosome localization and bioimaging of intracellular ALP in living cells. (C) 2021 Elsevier B.V. All rights reserved.
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