Investigation of spectrophotometric simultaneous absorption of Salmeterol and Fluticasone in Seroflo spray by continuous wavelet transform and radial basis function neural network methods

In this research, the simultaneous absorption of Salmeterol (SAL) and Fluticasone (FLU) in Seroflo spray was investigated using a spectrophotometric device via employing continuous wavelet transform (CWT) and radial basis function neural network (RBF-NN) methods. Root mean square error (RMSE) related to the RBF model was obtained 3.17 x 10(-13) and 1.41 x 10(-13) for SAL and FLU, respectively. Limit of detection (LOD) and limit of quantification (LOQ) corresponding to the CWT method were 0.004, 0.280 mu g/mL, and 0.431, 0.479 mu g/mL for SAL and FLU, respectively. Root mean square error (RMSE) of SAL and FLU was obtained 3.17 x 10(-13) and 1.41 x 10(-13), respectively in RBF-NN method. In the end, the results obtained from all methods were compared with the high-performance liquid chromatography (HPLC) as a reference method. According to the one-way analysis of variance with a 95% confidence level, there is no significant difference between the proposed techniques and HPLC. Therefore, chemometrics methods are sufficiently accurate, as the reference method for the analysis of drugs. The suggested methods are simple, fast, and cheap. Also, there is no need for pre-preparation steps. These methods can be used for quality control laboratories in the pharmaceutical industry. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

This study investigated the simultaneous absorption of SAL and FLU in Seroflo spray using CWT and RBF-NN methods. The RMSE and LOD/LOQ values obtained showed the accuracy and sensitivity of the chemometrics methods, which were comparable to HPLC as a reference method for drug analysis in pharmaceutical quality control laboratories.