Article
Oncology
Diana Rose E. Ranoa, Preeti Sharma, Claire P. Schane, Amber N. Lewis, Edward Valdez, Venkata V. V. R. Marada, Marlies Hager, Will Montgomery, Steven P. Wolf, Karin Schreiber, Hans Schreiber, Keith Bailey, Timothy M. Fan, Paul J. Hergenrother, Edward J. Roy, David M. Kranz
Summary: The use of CAR-T cell therapy against blood cancers has been successful, but its efficacy against solid tumors has been limited. By using a mouse ovarian cancer cell line, researchers found that CAR-T cell therapy can effectively inhibit the growth of solid tumors, with the most effective CAR having the highest affinity for the antigen.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Oncology
Fengguang Guo, Jugal K. Das, Koichi S. Kobayashi, Qing-Ming Qin, Thomas A. Ficht, Robert C. Alaniz, Jianxun Song, Paul De Figueiredo
Summary: This study demonstrates that live attenuated bacterial treatment can improve antitumor immunity by remodeling the tumor microenvironment, overcoming cancer resistance to chimeric antigen receptor T-cell therapy.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Review
Immunology
Stefanie R. Bailey, Trisha R. Berger, Charlotte Graham, Rebecca C. Larson, Marcela V. Maus
Summary: Cell-based therapies using CAR T cells have shown significant efficacy in hematologic malignancies and have the potential to induce remission in autoimmune diseases. However, several obstacles including toxicity, antigen identification, solid tumor functionality, and manufacturing complexity and cost need to be addressed for wider clinical utility. Breaking these barriers is essential to improve clinical responses and make this life-saving therapy accessible to a larger patient population.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Review
Oncology
Zhaojun Pang, Zhongyi Wang, Fengqi Li, Chunjing Feng, Xin Mu
Summary: CAR-NK therapy, as a promising cancer treatment, attracts attention due to its lack of adverse effects observed in CAR-T therapies and its effectiveness in killing cancer cells. However, CAR-NK therapy faces challenges such as difficulties in transduction and expansion of NK cells, as well as time, cost, and potential biohazard issues.
Review
Pharmacology & Pharmacy
Javad Masoumi, Abdollah Jafarzadeh, Jalal Abdolalizadeh, Haroon Khan, Jeandet Philippe, Hamed Mirzaei, Hamid Reza Mirzaei
Summary: CSCs are cells with self-renewal ability that initiate tumors and are potential targets for novel anticancer therapeutic agents. CAR-T cells, engineered T cells expressing an artificial receptor specific for TAAs, have shown higher efficiency in cancer treatment by accurately targeting and killing cancer cells.
ACTA PHARMACEUTICA SINICA B
(2021)
Review
Oncology
Kevin Pan, Hizra Farrukh, Veera Chandra Sekhar Reddy Chittepu, Huihong Xu, Chong-xian Pan, Zheng Zhu
Summary: CAR immunotherapy has achieved significant progress in hematological malignancies, but faces challenges in solid tumors. CAR NK cells and CAR macrophages have advantages and limitations. Developing CAR macrophages could be a strategy to overcome hurdles associated with CAR therapy in solid tumors.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Oncology
Celia Martin-Otal, Aritz Lasarte-Cia, Diego Serrano, Noelia Casares, Enrique Conde, Flor Navarro, Ines Sanchez-Moreno, Marta Gorraiz, Patricia Sarrion, Alfonso Calvo, Carlos E. De Andrea, Jose Echeveste, Amaia Vilas, Juan Roberto Rodriguez-Madoz, Jesus San Miguel, Felipe Prosper, Sandra Hervas-Stubbs, Juan Jose Lasarte, Teresa Lozano
Summary: This study demonstrates the feasibility and efficacy of targeting the tumor-specific fibronectin splice variant EDA with CAR-T cells, providing a potential therapeutic option for different types of cancer.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Oncology
Rasmus Erik Johansson Mortensen, Morten Orebo Holmstrom, Thomas Landkildehus Lisle, Jane P. Hasselby, Gro L. Willemoe, Ozcan Met, Inge Marie Svane, Julia Johansen, Dorte L. Nielsen, Inna M. Chen, Mads Hald Andersen
Summary: This study investigated the significance of TGF-beta-specific T-cell immunity in patients with pancreatic cancer treated with ICI combined with radiotherapy. The results showed that patients with a strong TGF-beta-specific immune response had longer progression-free and overall survival compared to those with a weak or no response. It was also found that TGF-beta-specific T cells could recognize and enhance immune responses. Thus, combining TGF-beta vaccination with ICI/radiotherapy may benefit patients with pancreatic cancer.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Review
Oncology
Sara Pagotto, Pasquale Simeone, Davide Brocco, Giulia Catitti, Domenico De Bellis, Simone Vespa, Natalia Di Pietro, Lisa Marinelli, Antonio Di Stefano, Serena Veschi, Laura De Lellis, Fabio Verginelli, Francesco Kaitsas, Manuela Iezzi, Assunta Pandolfi, Rosa Visone, Nicola Tinari, Ignazio Caruana, Mauro Di Ianni, Alessandro Cama, Paola Lanuti, Rosalba Florio
Summary: This review highlights the potential of CAR-T-derived extracellular vesicles (EVs) as therapeutic agents in tumors. CAR-T lymphocytes have shown potential effectiveness in immunotherapy against refractory neoplastic diseases. However, physical barriers, hostile microenvironment, and tumor heterogeneity limit their efficacy against solid tumors. The use of CAR-T-derived EVs as therapeutic agents may enhance CAR-T homing and overcome some adverse effects.
Review
Immunology
Long Chen, Fukun Chen, Huatao Niu, Jindan Li, Yongzhu Pu, Conghui Yang, Yue Wang, Rong Huang, Ke Li, Yujie Lei, Yunchao Huang
Summary: CAR-T cell therapy is a novel immunotherapy strategy that has been successfully used against both hematological tumors and solid tumors. Despite some challenges, CAR-T cell treatment still holds great promise.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Oncology
Ran Chen, Lei Chen, Chaoqun Wang, Hua Zhu, Lijuan Gu, Yuntao Li, Xiaoxing Xiong, Gang Chen, Zhihong Jian
Summary: This paper discusses the limitations and challenges of CAR-T cell therapy in solid tumors, introduces the latest therapeutic strategies and management decisions to overcome these obstacles, aiming to facilitate the widespread use of CAR-T immunotherapy.
FRONTIERS IN ONCOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Karin Teppert, Xueting Wang, Kathleen Anders, Cesar Evaristo, Dominik Lock, Annette Kuenkele
Summary: This article provides a comprehensive review comparing the architecture, synapse formation, and signaling of TCRs and CARs, highlighting the similarities and differences, as well as the knowns and unknowns. Additionally, the current status of combining CAR and TCR technologies is discussed, advocating a shift in perspective from TCR versus CAR to TCR and CAR.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Francois Anna, Elodie Bole-Richard, Joel LeMaoult, Marie Escande, Martin Lecomte, Jean-Made Certoux, Philippe Souque, Francine Garnache, Olivier Adotevi, Pierre Langlade-Demoyen, Maria Loustau, Julien Caumartin
Summary: CAR-T cell immunotherapy is a breakthrough in treating hematological malignancies, but the lack of tumor-specific antigens and immunosuppressive tumor microenvironments remain major hurdles. A new study shows that HLA-G, acting as both an immune checkpoint and a tumor-specific antigen, could be a promising target for CAR-T cell therapy.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Review
Medicine, Research & Experimental
Gunjan Dagar, Ashna Gupta, Tariq Masoodi, Sabah Nisar, Maysolun Merhi, Sheema Hashem, Ravi Chauhan, Manisha Dagar, Sameer Mirza, Puneet Bagga, Rakesh Kumar, Ammira S. Al-Shabeeb Akil, Muzafar A. Macha, Mohammad Haris, Shahab Uddin, Mayank Singh, Ajaz A. Bhat
Summary: Traditional cancer treatments use nonspecific drugs and monoclonal antibodies, while CAR-T cell therapy leverages the immune system's T-cells to recognize and attack tumor cells. Despite success in blood cancers, CAR-T technology faces challenges in solid tumors such as lack of reliable tumor-associated antigens and immunosuppressive tumor environments. To overcome these challenges, current research aims to identify reliable tumor-associated antigens and develop cost-effective, tumor microenvironment-specific CAR-T cells.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Review
Immunology
Prameela Kandra, Rajender Nandigama, Bastian Eul, Magdalena Huber, Sebastian Kobold, Werner Seeger, Friedrich Grimminger, Rajkumar Savai
Summary: Current treatments for lung cancer have not substantially improved the prognosis. CAR-T cell immunotherapy has shown promising results in leukemia but faces challenges in solid tumors like lung cancer, including antigen heterogeneity, the immunosuppressive tumor microenvironment, and tumor penetration by CAR-T cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Anastasiia Sokolova, Milos Galic
Summary: Mechanical forces on the plasma membrane change cell shape and trigger the enrichment of curvature-sensitive molecules. Some of these molecules can not only detect membrane deformation, but also alter the forces that caused the shape changes. The formation of curvature-dependent self-organizing signaling circuits is a result of this dual ability. However, the influence of auxiliary factors on these cell-autonomous circuits remains unclear.
BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Andrey S. Drozdov, Kristina S. Komarova, Elizaveta N. Mochalova, Elena N. Komedchikova, Victoria O. Shipunova, Maxim P. Nikitin
Summary: Introduces a method to synthesize multifunctional nanomaterials through biomimetic polymerization of dopamine derivatives. By combining magnetite nanoparticles with dopamine conjugates, multifunctional magneto-fluorescent nanocomposites were synthesized in one step. The obtained material showed excellent biocompatibility and a typical biodistribution profile for nanosized formulations. The synthesized systems were conjugated with antibodies against HER2 to improve selectivity towards HER2-positive cancer cells, allowing for dual magneto-optical in vivo studies or targeted drug delivery. This synthetic strategy can be applied to create various multifunctional hybrid nanomaterials under mild conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Tianqi Xu, Alexey Schulga, Elena Konovalova, Sara S. Rinne, Hongchao Zhang, Olga Vorontsova, Anna Orlova, Sergey M. Deyev, Vladimir Tolmachev, Anzhelika Vorobyeva
Summary: Pancreatic cancer (PC) is an aggressive malignancy, and targeted therapies could improve its treatment. Overexpression of HER3 and EpCAM in PC is associated with poor prognosis. The study investigated whether co-targeting HER3 with MM-121 and EpCAM with Ec1-LoPE could enhance therapeutic efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Nataliia A. Petrunina, Alina S. Shtork, Maria M. Lukina, Vladimir B. Tsvetkov, Yuri M. Khodarovich, Alexey V. Feofanov, Anastasia M. Moysenovich, Eugene G. Maksimov, Victoria O. Shipunova, Timofei S. Zatsepin, Alexandra N. Bogomazova, Victoria O. Shender, Andrey V. Aralov, Maria A. Lagarkova, Anna M. Varizhuk
Summary: DNA-intercalated motifs serve as convenient scaffolds for designing pH-responsive nanomachines, including biocompatible pH sensors. By using a simple unimolecular dual-labeled iM scaffold and modifying it with abasic or alkyl linkers, the thermal stability and pH-induced conformational transitions of the iM were improved.
Article
Pharmacology & Pharmacy
Elena N. Komedchikova, Olga A. Kolesnikova, Ekaterina D. Tereshina, Polina A. Kotelnikova, Anna S. Sogomonyan, Alexey V. Stepanov, Sergey M. Deyev, Maxim P. Nikitin, Victoria O. Shipunova
Summary: Nanoparticle-based chemotherapy is an effective approach to cancer diagnostics and therapy, but efficient tumor targeting remains a challenge. A two-step targeted drug delivery strategy (DDS) using non-toxic and toxic modules can lower dose and systemic toxicity. In this study, we compared one-step and two-step DDS using chemotherapy loaded PLGA nanoparticles and barnase*barstar interface. We found that the two-step DDS significantly improved the performance of chemotherapy-loaded nanocarriers, making it a promising strategy for targeted anti-cancer drugs.
Article
Biochemistry & Molecular Biology
Marcus Moldenhauer, Hsueh-Wei Tseng, Anastasia Kraskov, Neslihan N. Tavraz, Igor A. Yaroshevich, Peter Hildebrandt, Nikolai N. Sluchanko, Georg A. Hochberg, Lars-Oliver Essen, Nediljko Budisa, Lukas Korf, Eugene G. Maksimov, Thomas Friedrich
Summary: In this study, the effects of a single hydrogen bond absence on the spectroscopic and thermodynamic properties of OCP were investigated by replacing Trp288 in Synechocystis OCP. The results provide insights into the general principles underlying the design of photoreceptors by natural evolution.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Alexandra Bogdanova, Elizaveta Pavlova, Anna Polyanskaya, Marina Volkova, Elena Biryukova, Gleb Filkov, Alexander Trofimenko, Mikhail Durymanov, Dmitry Klinov, Dmitry Bagrov
Summary: Biocompatible polyesters are commonly used in biomedical applications and can be tuned by blending with proteins. This usually improves hydrophilicity and cell adhesion but reduces mechanical properties. In this study, a 9:1 PLA:gelatin blend was found to accelerate the biodegradation of electrospun PLA mats without affecting their strength and extensibility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Victoria O. Shipunova, Elena N. Komedchikova, Polina A. Kotelnikova, Maxim P. Nikitin, Sergey M. Deyev
Summary: Therapy for aggressive metastatic breast cancer remains a challenge, but researchers are exploring the development of chemotherapeutic nanoagents targeting cancer cell receptors. A two-step targeted delivery approach using HER2 protein and chemotherapy nanoparticles has shown to be more effective than a one-step strategy.
Article
Chemistry, Multidisciplinary
Gleb I. Tselikov, Artem Danilov, Victoria O. Shipunova, Sergey M. Deyev, Andrei V. Kabashin, Alexander N. Grigorenko
Summary: Optical biosensors have great potential in biomedical diagnostics, security, environmental, and food safety applications. However, the sensitivity of current plasmonic biosensors is limited. In this study, we propose a scissor effect in topologically dark metamaterials to achieve ultrahigh-amplitude sensitivity in biosensing events, solving the sensitivity limitation problem. By designing topologically dark metamaterials and fabricating them, we demonstrate the detection of folic acid with greatly improved sensitivity compared to previous optical counterparts.
Article
Automation & Control Systems
Igramotdin S. Aliiev, Roman I. Sivak, Oleg E. Markov, Vladymyr N. Levchenko
Summary: The article introduces a calculation algorithm that can assess the deformability of the workpiece and the value of the used plasticity resource in the process of combined extrusion. Experimental results confirm the advantages of the combined process in reducing power parameters and deformation unevenness.
INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Elizaveta N. Mochalova, Elena A. Egorova, Kristina S. Komarova, Victoria O. Shipunova, Nelli F. Khabibullina, Petr I. Nikitin, Maxim P. Nikitin
Summary: Recent developments in nanomedicine have introduced nanomaterials capable of recognizing and killing tumor cells with higher specificity. However, their clinical application is limited by their fast clearance from the bloodstream. MPS-cytoblockade shows promise in prolonging the circulation of nanoagents, but concerns have been raised about its potential health risks. This study suggests that the MPS-cytoblockade may be less harmful than liposomal anti-cancer drugs while significantly improving the efficacy of nanoagents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Vera L. Kovalenko, Olga A. Kolesnikova, Maxim P. Nikitin, Victoria O. Shipunova, Elena N. Komedchikova
Summary: Photothermal therapy is a highly efficient and minimally invasive treatment for tumors, and the properties of polymeric nanoparticles (NPs) used in this therapy are affected by the synthesis components. This study explores the impact of stabilizers and solvents on the properties of PLGA NPs for photothermal therapy. By encapsulating a phthalocyanine-based photosensitizer, promising photothermal properties were achieved in PLGA NPs synthesized using mQ water as a solvent and chitosan oligosaccharide lactate as a stabilizer. The resulting composite showed photoinduced cytotoxicity against EMT6/P cells and was suitable for bioimaging.
Article
Chemistry, Multidisciplinary
Irina N. Zavestovskaya, Anton L. Popov, Danil D. Kolmanovich, Gleb V. Tikhonowski, Andrei I. Pastukhov, Maxim S. Savinov, Pavel V. Shakhov, Julia S. Babkova, Anton A. A. Popov, Ivan V. Zelepukin, Maria S. Grigoryeva, Alexander E. Shemyakov, Sergey M. Klimentov, Vladimir A. Ryabov, Paras N. Prasad, Sergey M. Deyev, Andrei V. V. Kabashin
Summary: Proton therapy is a promising radiotherapy modality for deep-seated and unresectable tumors, and its efficiency can be enhanced by using boron-containing substances. In this study, elemental boron nanoparticles (B NPs) were explored as sensitizers for proton therapy enhancement. These B NPs were efficiently absorbed by cancer cells and showed no remarkable toxicity effects. Proton beam irradiation of B NPs also led to the generation of reactive oxygen species, suggesting a possible non-nuclear mechanism of cancer cell death. Overall, this study offers a new approach with potential clinical applications for proton beam-based cancer treatment.
Article
Chemistry, Analytical
Alexey M. Yashchenok, Vasiliy S. Chernyshev, Elena V. Konovalova, Roman Kholodenko, Ekaterina Tsydenzhapova, Victoria O. Shipunova, Alexey A. Schulga, Sergey M. Deyev, Dmitry A. Gorin
Summary: In this study, we propose a method for the isolation and detection of sEVs based on anti-CD63 aptamer conjugated magnetic beads and the presence of membrane proteins. We compare the surface markers of EpCAM and HER2 in sEVs derived from different cell lines and blood plasma. The results show high specificity and a shorter assay time.
ANALYSIS & SENSING
(2023)