RNA sequence and structure control assembly and function of RNA condensates

In vivo reconstitution finds multivalent RNA-RNA interactions as drivers of mesh-like condensates

(2021) Weirui Ma et al.   eLife

Recruitment of mRNAs to P granules by condensation with intrinsically-disordered proteins

(2020) Chih-Yung S Lee et al.   eLife

Sequence-Controlled Adhesion and Microemulsification in a Two-Phase System of DNA Liquid Droplets

(2020) Byoung-jin Jeon et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Prebiotically-relevant low polyion multivalency can improve functionality of membraneless compartments

(2020) Fatma Pir Cakmak et al.   Nature Communications

Template-directed RNA polymerization and enhanced ribozyme catalysis inside membraneless compartments formed by coacervates

(2019) Raghav R. Poudyal et al.   Nature Communications

A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos

(2019) Andrea Putnam et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties

(2019) Steven Boeynaems et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Polyanion-Assisted Ribozyme Catalysis Inside Complex Coacervates

(2019) Raghav R. Poudyal et al.   ACS Chemical Biology

G-quadruplex structures trigger RNA phase separation

(2019) Yueying Zhang et al.   NUCLEIC ACIDS RESEARCH

Divalent cations can control a switch-like behavior in heterotypic and homotypic RNA coacervates

(2019) Paulo L. Onuchic et al.   Scientific Reports

Physical Principles and Extant Biology Reveal Roles for RNA-Containing Membraneless Compartments in Origins of Life Chemistry

(2018) Raghav R. Poudyal et al.   BIOCHEMISTRY

RNA self-assembly contributes to stress granule formation and defining the stress granule transcriptome

(2018) Briana Van Treeck et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Variant Bacterial Riboswitches Associated with Nucleotide Hydrolase Genes Sense Nucleoside Diphosphates

(2018) Madeline E. Sherlock et al.   BIOCHEMISTRY

The molecular language of membraneless organelles

(2018) Edward Gomes et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Compartmentalised RNA catalysis in membrane-free coacervate protocells

(2018) Björn Drobot et al.   Nature Communications

DNA Local-Flexibility-Dependent Assembly of Phase-Separated Liquid Droplets

(2018) Anisha Shakya et al.   BIOPHYSICAL JOURNAL

Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif

(2018) Sandip A. Shelke et al.   Nature Communications

The Stress Granule Transcriptome Reveals Principles of mRNA Accumulation in Stress Granules

(2017) Anthony Khong et al.   MOLECULAR CELL

Metabolism of Free Guanidine in Bacteria Is Regulated by a Widespread Riboswitch Class

(2017) James W. Nelson et al.   MOLECULAR CELL

RNA phase transitions in repeat expansion disorders

(2017) Ankur Jain et al.   NATURE

Crystal structure and fluorescence properties of the iSpinach aptamer in complex with DFHBI

(2017) Pablo Fernandez-Millan et al.   RNA

ATP as a biological hydrotrope

(2017) Avinash Patel et al.   SCIENCE

Polar Positioning of Phase-Separated Liquid Compartments in Cells Regulated by an mRNA Competition Mechanism

(2016) Shambaditya Saha et al.   CELL

Polyamine/Nucleotide Coacervates Provide Strong Compartmentalization of Mg2+, Nucleotides, and RNA

(2016) Erica A. Frankel et al.   LANGMUIR

RNA-Based Coacervates as a Model for Membraneless Organelles: Formation, Properties, and Interfacial Liposome Assembly

(2016) William M. Aumiller et al.   LANGMUIR

Membraneless organelles can melt nucleic acid duplexes and act as biomolecular filters

(2016) Timothy J. Nott et al.   Nature Chemistry

Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3

(2016) Jarrett Smith et al.   eLife

Challenge of Mimicking the Influences of the Cellular Environment on RNA Structure by PEG-Induced Macromolecular Crowding

(2015) Jillian Tyrrell et al.   BIOCHEMISTRY

Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression

(2015) Hyejin Kim et al.   Scientific Reports

Broccoli: Rapid Selection of an RNA Mimic of Green Fluorescent Protein by Fluorescence-Based Selection and Directed Evolution

(2014) Grigory S. Filonov et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Rapid RNA Exchange in Aqueous Two-Phase System and Coacervate Droplets

(2014) Tony Z. Jia et al.   ORIGINS OF LIFE AND EVOLUTION OF BIOSPHERES

Polyethylene glycol binding alters human telomere G-quadruplex structure by conformational selection

(2013) Robert Buscaglia et al.   NUCLEIC ACIDS RESEARCH

Mechanisms of RNA-induced toxicity in CAG repeat disorders

(2013) R Nalavade et al.   Cell Death & Disease

R2R - software to speed the depiction of aesthetic consensus RNA secondary structures

(2011) Zasha Weinberg et al.   BMC BIOINFORMATICS

Peptide–nucleotide microdroplets as a step towards a membrane-free protocell model

(2011) Shogo Koga et al.   Nature Chemistry

NMR Detection of Structures in the HIV-1 5'-Leader RNA That Regulate Genome Packaging

(2011) K. Lu et al.   SCIENCE

NUPACK: Analysis and design of nucleic acid systems

(2010) Joseph N. Zadeh et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE