Synthesis of Silver Nanoparticle from Allium sativum as an Eco-Benign Agent for Biological Applications

The study focuses on the fabrication of silver nanoparticles (Ag NPs) using aqueous extracts of Allium sativum as reducing agents. Silver ion becomes a good carrier of bioactive compounds of Allium sativum, so their biochemical potential such as total phenolic and flavonoids were evaluated. Results showed that as a concentration increases TPC and TFC were also increased. Similar to their secondary metabolite, scavenging potential of Ag NPs of Allium sativum was also monitored. Green synthesized Ag NPs exhibited significant antibacterial activity 24 +/- 2.6, 22 +/- 1.4, 19 +/- 2.7 and 20 +/- 3.17 against B. subtilis, S. aureus, E. coli and P. multocida bacterial strains respectively. Moreover, Ag NPs of garlic did not show any mutagenicity against mutant strain of S. typhimurium TA98 & TA100. Brine shrimp lethality assay (BSLA) showed their dose dependent effect. Finally, green synthesized Ag NPs of garlic presented anti-proliferative effect against HEPG2 cell line.

Automated summary

This study focuses on the fabrication of silver nanoparticles using aqueous extracts of garlic as reducing agents and evaluates their biochemical potential and antibacterial activity. The results show that the silver nanoparticles exhibit significant antibacterial activity and anti-proliferative effects.