Cas11 enables genome engineering in human cells with compact CRISPR-Cas3 systems

Dual modes of CRISPR-associated transposon homing

(2021) Makoto Saito et al.   CELL

Programmable RNA targeting with the single-protein CRISPR effector Cas7-11

(2021) Ahsen Özcan et al.   NATURE

Genome editing in mammalian cells using the CRISPR type I-D nuclease

(2021) Keishi Osakabe et al.   NUCLEIC ACIDS RESEARCH

The gRAMP CRISPR-Cas effector is an RNA endonuclease complexed with a caspase-like peptidase

(2021) Sam P. B. van Beljouw et al.   SCIENCE

Characterization and applications of Type I CRISPR-Cas systems

(2020) Claudio Hidalgo-Cantabrana et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

The promise and challenge of therapeutic genome editing

(2020) Jennifer A. Doudna   NATURE

Endogenous Type I CRISPR-Cas: From Foreign DNA Defense to Prokaryotic Engineering

(2020) Yanli Zheng et al.   Frontiers in Bioengineering and Biotechnology

CRISPR-Based Therapeutic Genome Editing: Strategies and In Vivo Delivery by AAV Vectors

(2020) Dan Wang et al.   CELL

Multiplexed CRISPR technologies for gene editing and transcriptional regulation

(2020) Nicholas S. McCarty et al.   Nature Communications

Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

(2020) Andrew V. Anzalone et al.   NATURE BIOTECHNOLOGY

Repurposing type I–F CRISPR–Cas system as a transcriptional activation tool in human cells

(2020) Yuxi Chen et al.   Nature Communications

Chemistry of Class 1 CRISPR-Cas effectors: Binding, editing, and regulation

(2020) Tina Y. Liu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A compact Cascade–Cas3 system for targeted genome engineering

(2020) Bálint Csörgő et al.   NATURE METHODS

Diverse CRISPR-Cas Complexes Require Independent Translation of Small and Large Subunits from a Single Gene

(2020) Tess M. McBride et al.   MOLECULAR CELL

Structural basis for assembly of non-canonical small subunits into type I-C Cascade

(2020) Roisin E. O’Brien et al.   Nature Communications

Introducing a Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas

(2019) Adam E. Dolan et al.   MOLECULAR CELL

Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration

(2019) Sanne E. Klompe et al.   NATURE

Targeted transcriptional modulation with type I CRISPR–Cas systems in human cells

(2019) Adrian Pickar-Oliver et al.   NATURE BIOTECHNOLOGY

Harnessing type I CRISPR–Cas systems for genome engineering in human cells

(2019) Peter Cameron et al.   NATURE BIOTECHNOLOGY

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

(2019) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

CRISPR-Cas3 induces broad and unidirectional genome editing in human cells

(2019) Hiroyuki Morisaka et al.   Nature Communications

Delivering CRISPR: a review of the challenges and approaches

(2018) Christopher A. Lino et al.   DRUG DELIVERY

CRISPRCasFinder, an update of CRISRFinder, includes a portable version, enhanced performance and integrates search for Cas proteins

(2018) David Couvin et al.   NUCLEIC ACIDS RESEARCH

Structure basis for RNA-guided DNA degradation by Cascade and Cas3

(2018) Yibei Xiao et al.   SCIENCE

CRISPR-Cas guides the future of genetic engineering

(2018) Gavin J. Knott et al.   SCIENCE

Assembly and Translocation of a CRISPR-Cas Primed Acquisition Complex

(2018) Kaylee E. Dillard et al.   CELL

Recruitment of CRISPR-Cas systems by Tn7-like transposons

(2017) Joseph E. Peters et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni

(2017) Eunji Kim et al.   Nature Communications

Fusion guide RNAs for orthogonal gene manipulation with Cas9 and Cpf1

(2017) Jiyeon Kweon et al.   Nature Communications

Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector

(2017) Ziqing Liu et al.   Scientific Reports

DNA Targeting by a Minimal CRISPR RNA-Guided Cascade

(2016) Megan L. Hochstrasser et al.   MOLECULAR CELL

Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems

(2016) Ryan T. Leenay et al.   MOLECULAR CELL

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Multicolor CRISPR labeling of chromosomal loci in human cells

(2015) Hanhui Ma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli

(2014) Hongtu Zhao et al.   NATURE

Structures of CRISPR Cas3 offer mechanistic insights into Cascade-activated DNA unwinding and degradation

(2014) Yanwu Huo et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference

(2014) M. L. Hochstrasser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Crystal structure of a CRISPR RNA-guided surveillance complex bound to a ssDNA target

(2014) S. Mulepati et al.   SCIENCE

Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli

(2014) R. N. Jackson et al.   SCIENCE

In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus

(2013) Tomas Sinkunas et al.   EMBO JOURNAL

In VitroReconstitution of anEscherichia coliRNA-guided Immune System Reveals Unidirectional, ATP-dependent Degradation of DNA Target

(2013) Sabin Mulepati et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

(2013) Kevin M Esvelt et al.   NATURE METHODS

Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems

(2013) Ines Fonfara et al.   NUCLEIC ACIDS RESEARCH

OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects

(2013) Quentin Geissmann   PLoS One

CRISPR-Cas Systems in the Cyanobacterium Synechocystis sp. PCC6803 Exhibit Distinct Processing Pathways Involving at Least Two Cas6 and a Cmr2 Protein

(2013) Ingeborg Scholz et al.   PLoS One

CRISPRTarget

(2013) Ambarish Biswas et al.   RNA Biology

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

CRISPR Immunity Relies on the Consecutive Binding and Degradation of Negatively Supercoiled Invader DNA by Cascade and Cas3

(2012) Edze R. Westra et al.   MOLECULAR CELL

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Cas5d Protein Processes Pre-crRNA and Assembles into a Cascade-like Interference Complex in Subtype I-C/Dvulg CRISPR-Cas System

(2012) Ki Hyun Nam et al.   STRUCTURE

Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems

(2011) Kira S Makarova et al.   Biology Direct

Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system

(2011) Tomas Sinkunas et al.   EMBO JOURNAL

Structures of the RNA-guided surveillance complex from a bacterial immune system

(2011) Blake Wiedenheft et al.   NATURE

FastTree 2 – Approximately Maximum-Likelihood Trees for Large Alignments

(2010) Morgan N. Price et al.   PLoS One

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE

CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA

(2008) Luciano A. Marraffini et al.   SCIENCE