Article
Cell Biology
Ze Long, Pengcheng Dou, Weiliang Cai, Minzhi Mao, Ren Wu
Summary: High-throughput miRNA sequencing of osteoporosis from the GEO database identified miR-181a-5p as a differentially expressed microRNA. Modulation of miR-181a-5p levels in MC3T3-E1 cells affected osteogenic differentiation. Target gene prediction revealed BMP3 as a target of miR-181a-5p. Reduced expression of miR-181a-5p was validated in osteoporotic patients, implicating its importance in disease prevention.
Article
Dermatology
Vida Chitsazzadeh, Tran N. Nguyen, Alvaro de Mingo Pulido, Bruna B. Bittencourt, Lili Du, Charles H. Adelmann, Ivannie Ortiz Rivera, Kimberly A. Nguyen, Leah D. Guerra, Andrew Davis, Marco Napoli, Wencai Ma, Richard Eric Davis, Kimal Rajapakshe, Cristian Coarfa, Elsa R. Flores, Kenneth Y. Tsai
Summary: Upregulation of miR-181a promotes the development of cutaneous squamous cell carcinoma (cSCC) by targeting TGF beta R3, a component of TGF beta signaling. Overexpression of miR-181a or knockdown of TGF beta R3 significantly suppresses UV-induced apoptosis in HaCaT cells and normal human epidermal keratinocytes. Moreover, miR-181a overexpression or TGF beta R3 knockdown enhances the survival, migration, and invasion of tumor cells.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Multidisciplinary Sciences
Sisi Chen, Binyun Ma, Xue Li, Kailang Zhang, Yankai Wei, Bei Du, Xun Liu, Ruihua Wei, Xiaorong Li, Hong Nian
Summary: This study reveals that miR-181a-5p negatively regulates pathogenic Th17 cell responses by targeting AKT3 and activating FOXO3. Decreased miR-181a-5p and up-regulated AKT3 expression were found in uveitis patients. Intravitreal administration of miR-181a-5p mimics effectively attenuated clinical and pathological signs of established uveitis.
Article
Oncology
Qi Zhang, Changli Wang, Ruijia Li, Jingjing Liu, Jiyu Wang, Tianyi Wang, Bing Wang
Summary: BAP31 regulates colorectal cancer (CRC) angiogenesis by manipulating the tumor microenvironment. Exosomes from BAP31-overexpressing CRCs induce the transformation of normal fibroblasts into proangiogenic cancer-associated fibroblasts (CAFs). Moreover, miR-181a-5p, a microRNA present in the exosomes, directly targets the RECK gene and promotes endothelial cell angiogenesis.
FRONTIERS IN ONCOLOGY
(2023)
Article
Medicine, Research & Experimental
Yinghui Wang, Qiuju Mou, Zixin Zhu, Luqiang Zhao, Lili Zhu
Summary: The study investigated the role of MALAT1 in regulating activation and inflammation of hepatic stellate cells in liver fibrosis. Both MALAT1 and miR-181a-5p expression were associated with liver fibrosis severity and differentiation of patients. The MALAT1/miR-181a-5p axis may be involved in regulating collagen production and inflammation, suggesting a potential target for liver fibrosis treatment.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Qiaoyi Sun, Li Ma, Jing Qiao, Xing Wang, Jianguo Li, Yuxi Wang, Ailing Tan, Zihui Ye, Yukang Wu, Jiajie Xi, Jiuhong Kang
Summary: The expression level of miR-181a-5p was found to be decreased in the hippocampal NSCs of aged mice, and exogenous overexpression of miR-181a-5p promoted NSC proliferation and improved learning and memory impairments caused by aging. The mechanistic study revealed that miR-181a-5p targeted phosphatase and tensin homolog (PTEN), a negative regulator of the AKT signaling pathway, and knockdown of PTEN rescued the impairment of NSC proliferation by low miR-181a-5p levels. Furthermore, overexpression of miR-181a-5p in the dentate gyrus increased NSC proliferation and ameliorated learning and memory impairments in aged mice.
Article
Oncology
Rui Wang, Houda Tahiri, Chun Yang, Solange Landreville, Sonia Callejo, Pierre Hardy
Summary: Uveal melanoma (UM) is the most common primary intraocular malignant tumor type in adults. Previous studies have shown that GNAQ/11 mutations are present in more than 85% of patients with UM, indicating their importance in UM occurrence. In this study, miR-181a-5p was found to effectively inhibit the viability, proliferation, and colony formation of UM cells, as well as induce apoptosis. Silencing GNAQ or AKT3 mimicked the anti-tumor effects of miR-181a-5p, while overexpression of GNAQ or AKT3 rescued these effects. MiR-181a-5p also suppressed the expression and phosphorylation of members of the ERK and PI3K/AKT/mTOR signaling pathways.
AMERICAN JOURNAL OF CANCER RESEARCH
(2023)
Article
Multidisciplinary Sciences
Jessica A. Kotov, Ying Xu, Nicholas D. Carey, Jason G. Cyster
Summary: LT beta R overexpression enhances the formation of plasma cells and may play a significant role in the development of multiple myeloma.
Article
Cell Biology
Weijia Wang, Jing Liu, Kang Chen, Juan Wang, Qian Dong, Jinye Xie, Yong Yuan
Summary: MiR-17-5p is highly expressed in AML and is associated with disease progression and poor prognosis. Vitamin D modulates miR-17-5p and Beclin-1 to promote autophagy in AML cells.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Medicine, General & Internal
Dongliang Zhang, Minglei Hua, Nan Zhang
Summary: This study found that LINC01232 is upregulated in lung squamous cell carcinoma (LUSC) and promotes LUSC progression by modulating the miR-181a-5p/SMAD2 signaling pathway. These findings provide potential new drug targets for the treatment of LUSC.
AMERICAN JOURNAL OF THE MEDICAL SCIENCES
(2023)
Review
Immunology
Thomas Baranek, Carolina de Amat Herbozo, Thierry Mallevaey, Christophe Paget
Summary: Invariant natural killer T (iNKT) cells are important biomarkers and targets for immunotherapy. Recent studies have revealed the heterogeneity of iNKT cells and challenged our understanding of their development and differentiation. A new model is proposed to explain the classification and differentiation of iNKT cells.
TRENDS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Yan Li, Juan Wang, Wenyu Chen, Xiaoping Chen, Jianhua Wang
Summary: This study revealed the crucial role of STAT4 in the metastasis and invasion of ovarian cancer, especially under hypoxic conditions. MiR-200a was identified as a direct regulator of STAT4, influencing the progression of EMT in ovarian cancer cells. These findings suggest a potential therapeutic target for the treatment of ovarian cancer.
Article
Biochemistry & Molecular Biology
Yanting Tian, Ye Zhao, Yuhan Sun, Yousry A. El-Kassaby, Guoyong Song, Yueqi Mi, Juan Han, Yun Li
Summary: This study identified the PagGRF11 gene in poplar and found that overexpression of this gene leads to dwarfing, stem diameter increase, internode shortening, and larger leaf area in poplar. Further investigation revealed that PagGRF11 promotes the development of xylem by activating the expression of CCCH39.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Zhiyong Wu, Ming Li, Yunpeng Zhong, Lan Li, Dawei Cheng, Hong Gu, Xizhi Guo, Xiujuan Qi, Jinyong Chen
Summary: In this study, 41 expansin genes were identified in kiwifruit, and their evolutionary diversity and conservation were analyzed. Overexpression of the gene AcEXPA23 was found to increase the number of kiwifruit lateral roots.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Maria-Alexandra Papadimitriou, Aristea-Maria Papanota, Panagiotis G. Adamopoulos, Katerina-Marina Pilala, Christine-Ivy Liacos, Panagiotis Malandrakis, Nefeli Mavrianou-Koutsoukou, Dimitrios Patseas, Evangelos Eleutherakis-Papaiakovou, Maria Gavriatopoulou, Efstathios Kastritis, Margaritis Avgeris, Meletios-Athanasios Dimopoulos, Evangelos Terpos, Andreas Scorilas
Summary: Despite advances in MM therapy, relapse and treatment resistance are still obstacles. Research on miRNAs in MM patients showed miR-181a as a potential biomarker for risk-stratification and prognosis, with overexpression associated with shorter progression and worse survival. Integrating miR-181a with established markers improved risk-stratification and benefit for MM prognosis.
BRITISH JOURNAL OF CANCER
(2022)
Article
Genetics & Heredity
Tiago Amado, Ana Amorim, Francisco J. Enguita, Paula Romero, Daniel Inacio, Marta Pires de Miranda, Samantha J. Winter, J. Pedro Simas, Andreas Krueger, Nina Schmolka, Bruno Silva-Santos, Anita Q. Gomes
JOURNAL OF MOLECULAR MEDICINE-JMM
(2020)
Review
Biochemistry & Molecular Biology
Zoe Grewers, Andreas Krueger
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Immunology
Marc Permanyer, Berislav Bosnjak, Silke Glage, Michaela Friedrichsen, Stefan Floess, Jochen Huehn, Gwendolyn E. Patzer, Ivan Odak, Nadine Eckert, Razieh Zargari, Laura Ospina-Quintero, Hristo Georgiev, Reinhold Foerster
Summary: Signaling through IL-2R is crucial for the identity and function of Treg cells, especially in inflammatory environments. Research on mutant mice revealed that impaired IL-2R signaling significantly affects the numbers, function, and subset balance of Treg cells.
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Immunology
S. Thera Lee, Hristo Georgiev, Elise R. Breed, Roland Ruscher, Kristin A. Hogquist
Summary: CD8 α α intestinal intraepithelial lymphocytes (IEL) mainly originate from thymic precursors, with development inhibited by CD80 and CD86, and conventional DCs playing a significant role. Thymic hematopoietic APC show heterogeneity in expression, but exhibit functional redundancy, with no specialized APC subset promoting the CD8 α α IEL fate.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Review
Physics, Multidisciplinary
Philippe A. Robert, Heike Kunze-Schumacher, Victor Greiff, Andreas Krueger
Summary: The thymus is crucial for the development of T cells, which play a key role in orchestrating the adaptive immune response. Experimental strategies and mathematical modeling have been used to understand the dynamic properties of T-cell development and the challenges of estimating in vivo cellular dynamics for achieving a next-generation multi-scale picture of T-cell development.
Article
Multidisciplinary Sciences
Hristo Georgiev, Changwei Peng, Matthew A. Huggins, Stephen C. Jameson, Kristin A. Hogquist
Summary: Conventional T cell subsets are selected in the thymus by peptide bearing MHC expressed by cortical epithelial cells, while cortical thymocytes express non-peptide bearing MHC molecules including CD1d and MR1 and select iNKT and MAIT cell populations respectively. The authors have generated a novel inducible MHC class-I transactivator murine system and suggest the absence of peptide-MHC on thymocytes is involved in the selection of non-peptide specific lymphocytes.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Nadezhda Todorova, Miroslav Rangelov, Vanya Bogoeva, Vishnya Stoyanova, Anna Yordanova, Ginka Nikolova, Hristo Georgiev, Daniela Dimitrova, Safa Mohedin, Katerina Stoyanova, Ivanka Tsacheva
Summary: The study investigated the structural features of autoepitopes recognized by anti-C1q antibodies in Lupus Nephritis (LN) sera, and identified monoclonal scFv A1 as a potent inhibitor of C1q recognition by lupus autoantibodies. The 3D model of A1 revealed CDR similarity to the apical region of gC1q, mapping a globular autoepitope of C1q for the first time. Other potential inhibitors among the selected recombinant antibodies were monoclonal scFv F6, F9, and A12.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Changwei Peng, Matthew A. Huggins, Kelsey M. Wanhainen, Todd P. Knutson, Hanbin Lu, Hristo Georgiev, Kristen L. Mittelsteadt, Nicholas N. Jarjour, Haiguang Wang, Kristin A. Hogquist, Daniel J. Campbell, Henrique Borges da Silva, Stephen C. Jameson
Summary: ICOS signaling pathway plays a critical role in the generation of CD8+ tissue-resident memory (Trm) T cells, promoting their production but not affecting their maintenance. This study reveals the importance of costimulatory signals in the generation of tissue-resident populations.
Article
Immunology
Berislav Bosnjak, Ivan Odak, Joana Barros-Martins, Inga Sandrock, Swantje I. Hammerschmidt, Marc Permanyer, Gwendolyn E. Patzer, Hristo Greorgiev, Rodrigo Gutierrez Jauregui, Alina Tscherne, Jan Hendrik Schwarz, Georgia Kalodimou, George Ssebyatika, Malgorzata Ciurkiewicz, Stefanie Willenzon, Anja Bubke, Jasmin Ristenpart, Christiane Ritter, Tamara Tuchel, Christian Meyer zu Natrup, Dai-Lun Shin, Sabrina Clever, Leonard Limpinsel, Wolfgang Baumgaertner, Thomas Krey, Asisa Volz, Gerd Sutter, Reinhold Foerster
Summary: The study demonstrates that intranasal delivery of MVA-SARS-2-S effectively induces pulmonary spike-specific CD8(+) T cells, albeit with restricted production of neutralizing antibodies. Intranasal booster vaccine delivery is crucial for massive expansion of spike-specific CD8(+) T cells in systemic and lung tissues, as well as the development of Th1 - but not Th2 - CD4(+) T cells, with high levels of IgG and IgA anti-spike antibodies capable of neutralizing all current SARS-CoV-2 variants of concern.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Andreas Krueger, Marcin Lyszkiewicz, Vigo Heissmeyer
Summary: This article summarizes recent advances in understanding the post-transcriptional regulation of T-cell development, focusing on microRNAs and RNA-binding proteins. It highlights the current challenges and proposes how they can be overcome with evolving sophisticated methodology to achieve a comprehensive mechanistic understanding of the regulatory networks operating in gene expression programs during T-cell development.
IMMUNOLOGY LETTERS
(2022)
Article
Cell Biology
Berislav Bosnjak, Elisa Henze, Yvonne Lueder, Kim Thi Hoang Do, Alaleh Rezalotfi, Berislav Cuvalo, Christiane Ritter, Anja Schimrock, Stefanie Willenzon, Hristo Georgiev, Lea Fritz, Melanie Galla, Karen Wagner, Martin Messerle, Reinhold Foerster
Summary: Murine cytomegalovirus (MCMV) infection of macrophages depends on MCMV-encoded chemokine 2 (MCK2), while infection of fibroblasts is independent of MCK2. Recent research shows that both cell types require neuropilin 1 for MCMV infection. CRISPR screening identifies MHC class Ia/b-2-microglobulin (B2m) expression as necessary for MCK2-dependent infection.
Article
Immunology
Georgia Papadogianni, Inga Ravens, Ahmed Hassan, Andrew Flatley, Regina Feederle, Guenter Bernhardt, Hristo Georgiev
Summary: Neuritin is a crucial neurotrophic factor involved in neuronal development and plasticity, as well as endothelial angiogenesis, cell migration, tumor growth, and antibody production. Monoclonal mouse anti-mouse neuritin antibodies were generated by immunizing knock-out mice with two different neuritin-derived peptides. These antibodies can recognize neuritin in various species, including humans, and are useful for studying neuritin expression and function, particularly in murine follicular regulatory T cells and germinal center B cells.
Article
Cell Biology
Tao Yang, Joana Barros-Martins, Ziqing Wang, Melanie Wencker, Jiang Zhang, Justine Smout, Prerna Gambhir, Anika Janssen, Hristo Georgiev, Anja Schimrock, Ximena Leon-Lara, Siegfried Weiss, Jochen Huehn, Immo Prinz, Andreas Krueger, Reinhold Foerster, Thierry Walzer, Sarina Ravens
Summary: This study investigates the development of Vy4 T cells in the adult thymus using a mouse model and single-cell chromatin state analysis. The results reveal that the adult-thymus-derived Vy4 T cells have the molecular programs to become IL-17 producers, but their IL-17 production capabilities are reduced and they rarely reach the periphery. The study also identifies Zeb1 as a potential regulator of y817 cells.
Article
Biochemistry & Molecular Biology
Georgia Papadogianni, Inga Ravens, Ahmed Hassan, Oliver Dittrich-Breiholz, Gunter Bernhardt, Hristo Georgiev
Summary: Invariant natural killer T (iNKT) cells develop in the thymus and then migrate to peripheral tissues and organs, adapting phenotypically and functionally to the tissue environment. A study comparing CD4(+) iNKT1 cells isolated from the liver and spleen found minimal genuine transcriptomic differences, with the method of cell isolation affecting the transcriptomic signature. Cells isolated via enzymatic digestion of tissue showed upregulation of stress response genes compared to cells isolated in cold conditions, emphasizing the importance of considering cell preparation history in expression profile comparisons.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2021)
Article
Immunology
Georgia Papadogianni, Inga Ravens, Oliver Dittrich-Breiholz, Guenter Bernhardt, Hristo Georgiev
FRONTIERS IN IMMUNOLOGY
(2020)