Article
Pharmacology & Pharmacy
Hiroki Hashizume, Tatsuki Fukami, Kanji Mishima, Hiroshi Arakawa, Kenji Mishiro, Yongjie Zhang, Masataka Nakano, Miki Nakajima
Summary: This study aimed to clarify the human ACS isoforms responsible for CoA-conjugation of NSAIDs by considering the hepatic expression levels of ACS isoforms. Among the 10 types of NSAIDs studied, propionic acid-class NSAIDs were found to be conjugated with CoA in the human liver, while NSAIDs in other classes did not exhibit this reaction. ACSL1 was identified as the most highly expressed ACS isoform in the human liver, and could catalyze the CoA conjugation of propionic acid-class NSAIDs, which may lead to toxicity through protein adduct formation.
BIOCHEMICAL PHARMACOLOGY
(2021)
Review
Pharmacology & Pharmacy
Jun Hou, Changqing Jiang, Xudong Wen, Chengming Li, Shiqiang Xiong, Tian Yue, Pan Long, Jianyou Shi, Zhen Zhang
Summary: Cancer is a major global health problem, and ACSL4 has different roles in different cancer cells, both inhibiting cancer cell progression and potentially being associated with tumor cell proliferation, migration, and invasion.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biology
Yu Meng, Cheryl Ingram-Smith, Oly Ahmed, Kerry Smith
Summary: By investigating the roles of active site residues in CoA binding in acetyl-CoA synthetase (Acs) and a medium-chain acyl-CoA synthetase (Macs), it was found that certain residues play a critical role in CoA binding and catalysis, revealing the active site architecture of this important enzyme superfamily.
Article
Biochemistry & Molecular Biology
Hiroshi Kuwata, Yuki Tomitsuka, Emiko Yoda, Shuntaro Hara
Summary: In this study, ACSL4 knockdown was found to decrease the levels of PUFA-derived acyl-CoA, particularly leading to a decrease in DHA-containing phospholipids. This inhibition further suppressed cell death induced by ferroptosis.
BIOSCIENCE REPORTS
(2022)
Article
Food Science & Technology
Katarzyna Jasieniecka-Gazarkiewicz, Sylwia Klinska-Bachor, Antoni Banas
Summary: This study investigated the impact of different acyl-CoAs on the remodeling efficiency of PC and PE mediated by acyl-CoA:lysophospholipid acyltransferases (LPLAT). Microsomal fractions from yeast & UDelta;ALE1 mutant transformed with plasmids encoding AtLPCAT or ALE1 genes were used. Results showed that the acyl-CoAs had different effects on the remodeling intensity of PC and PE mediated by the tested acyltransferases. Additionally, the presence of acyl-CoA in the reaction mixture influenced the remodeling processes through other reactions carried out by the enzymes.
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Runyi Huang, Wenli Yu, Rongzhen Zhang, Yan Xu
Summary: This study developed an efficient method for producing malonyl-CoA by screening the ACS gene from Streptomyces sp. The newly characterized ACS enzyme showed high conversion rate and yield of malonyl-CoA under optimized conditions.
PROCESS BIOCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Mengmeng Zheng, Jun Zhang, Wan Zhang, Lu Yang, Xiaoli Yan, Wenya Tian, Zhihao Liu, Zhi Lin, Zixin Deng, Xudong Qu
Summary: In this study, an ACS enzyme was improved through protein engineering, leading to enhanced activity in synthesizing acyl-CoAs. By combining it with carboxylases, several novel antimycin analogues were successfully produced through a modified biosynthetic pathway. This study expands the catalytic mode of ACSs and provides an important tool for the biosynthesis of acyl-CoA-derived natural products.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Endocrinology & Metabolism
Lili Zhang, Kazanna C. Hames, Michael D. Jensen
Summary: This study showed that differences in enzyme activity in adipose tissue may influence direct fatty acid storage, and physically active adults have lower rates of FFA storage.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Biochemistry & Molecular Biology
Hiroshi Kuwata, Eriko Nakatani, Yuki Tomitsuka, Tsubasa Ochiai, Yuka Sasaki, Emiko Yoda, Shuntaro Hara
Summary: The deficiency of ACSL4 increases susceptibility to endotoxin, possibly by overproduction of cyclooxygenase-derived eicosanoids.
Article
Biochemistry & Molecular Biology
Eden M. Gallegos, Tanner D. Reed, Forge A. Mathes, Nelson V. Guevara, David B. Neau, Wei Huang, Marcia E. Newcomer, Nathaniel C. Gilbert
Summary: This study reveals two distinct conformations of 5-lipoxygenase (5-LOX) in its catalytic reaction through structural and dynamic experiments. By strategic mutations, researchers successfully unlocked a more open conformation and improved the enzyme's activity. These findings are of great importance for understanding the synthesis mechanism of leukotrienes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Qing Zhang, Deqiang Yao, Bing Rao, Liyan Jian, Yang Chen, Kexin Hu, Ying Xia, Shaobai Li, Yafeng Shen, An Qin, Jie Zhao, Lu Zhou, Ming Lei, Xian-Cheng Jiang, Yu Cao
Summary: Phosphatidylcholine (PC) undergoes re-acylation catalyzed by lysophosphatidylcholine acyltransferases (LPCAT), with LPCAT3 introducing polyunsaturated acyl to modulate membrane fluidity and membrane protein functions. The structures of LPCAT3 reveal a reaction chamber and tunnels for substrate positioning, providing insights into the re-acylation mechanism.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Masato Ikeda, Keisuke Takahashi, Tatsunori Ohtake, Ryosuke Imoto, Haruka Kawakami, Mikiro Hayashi, Seiki Takeno
Summary: Recent research found a unique mechanism of lipid homeostasis in Corynebacterium glutamicum, involving a futile cycle of acyl-CoA hydrolysis and resynthesis mediated by acyl-CoA thioesterase (Tes) and acyl-CoA synthetase (FadD). Engineering this cycle in a high-fatty-acid producer significantly increases production, offering a useful strategy for improving fatty acid production in this industrially important microorganism.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yongjie Ma, Junyi Zha, XiangKun Yang, Qianjin Li, Qingfu Zhang, Amelia Yin, Zanna Beharry, Hanwen Huang, Jiaoti Huang, Michael Bartlett, Kaixiong Ye, Hang Yin, Houjian Cai
Summary: The study revealed that ACSL1 is highly expressed in prostate tumors, promoting the generation of fatty acyl-CoAs and facilitating cancer cell growth. ACSL1 modulates mitochondrial respiration, beta-oxidation, and ATP production, impacting fatty acid metabolism and prostate cancer progression.
Article
Biochemistry & Molecular Biology
Yuki Tomitsuka, Hiroki Imaeda, Haruka Ito, Isaki Asou, Masayuki Ohbayashi, Fumihiro Ishikawa, Hiroshi Kuwata, Shuntaro Hara
Summary: Long-chain acyl-CoA synthetase 4 (ACSL4) converts polyunsaturated fatty acids (PUFAs) into their acyl-CoAs and plays a crucial role in maintaining PUFA-containing membrane phospholipids. In this study, ACSL4 deficiency was found to attenuate pulmonary toxic chemical-induced lung injury and reduce mortality in mice. The results also showed that ACSL4 deficiency suppressed inflammation and neutrophil migration, as well as lipid peroxidation in the lung.
Article
Fisheries
Fang Chen, Xiaoping Huang, Hangbo Zhu, Yuanyou Li, Chao Xu, Dizhi Xie
Summary: This study investigated the molecular characterization and expression analysis of the acsl6 gene in the marine fish golden pompano (Trachinotus ovatus). The results showed that acsl6 is involved in the enrichment of DHA and over-expression of acsl6 can increase cellular DHA contents.
Editorial Material
Medicine, Research & Experimental
Matthew Spite
JOURNAL OF CLINICAL INVESTIGATION
(2019)
Article
Immunology
Nikolas Giannakis, Brian E. Sansbury, Andreas Patsalos, Tristan T. Hays, Colin O. Riley, Xianlin Han, Matthew Spite, Laszlo Nagy
Correction
Immunology
Nikolas Giannakis, Brian E. Sansbury, Andreas Patsalos, Tristan T. Hays, Colin O. Riley, Xianlin Han, Matthew Spite, Laszlo Nagy
Article
Multidisciplinary Sciences
Brian E. Sansbury, Xiaofeng Li, Blenda Wong, Andreas Patsalos, Nikolas Giannakis, Michael J. Zhang, Laszlo Nagy, Matthew Spite
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Multidisciplinary Sciences
Andrew L. Wishart, Sydney J. Conner, Justinne R. Guarin, Jackson P. Fatherree, Yifan Peng, Rachel A. McGinn, Rebecca Crews, Stephen P. Naber, Martin Hunter, Andrew S. Greenberg, Madeleine J. Oudin
Article
Hematology
Zeinab Hosseini, Michael Marinello, Christa Decker, Brian E. Sansbury, Sudeshna Sadhu, Brennan D. Gerlach, Ramon Bossardi Ramos, Alejandro P. Adam, Matthew Spite, Gabrielle Fredman
Summary: The study found that necroptotic cells impact resolution programs in atherosclerotic plaques and impair macrophages' clearance abilities. RvD1 may serve as a potential therapeutic strategy to limit necroptotic cells.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Pathology
Brian E. Sansbury, Xiaofeng Li, Blenda Wong, Colin O. Riley, Ashley E. Shay, Robert Nshimiyimana, Nicos A. Petasis, Charles N. Serhan, Matthew Spite
Summary: The study identified a novel regulator, PCTR1, which enhances human keratinocyte migration and accelerates wound closure in mice, showing potential for therapeutic management of delayed tissue repair and infection.
AMERICAN JOURNAL OF PATHOLOGY
(2021)
Article
Immunology
Sudeshna Sadhu, Christa Decker, Brian E. Sansbury, Michael Marinello, Allison Seyfried, Jennifer Howard, Masayuki Mori, Zeinab Hosseini, Thilaka Arunachalam, Aloke Finn, John M. Lamar, David Jourd'heuil, Liang Guo, Katherine C. MacNamara, Matthew Spite, Gabrielle Fredman
Summary: Radiation delays tissue repair mechanisms, leading to macrophage senescence and efferocytosis defects. Treatment with RvD1 promotes resolution and restores efferocytosis, providing a potential therapeutic strategy to limit radiation-induced tissue damage.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Endocrinology & Metabolism
Satoru Sugimoto, Hebe Agustina Mena, Brian E. Sansbury, Shio Kobayashi, Tadataka Tsuji, Chih-Hao Wang, Xuanzhi Yin, Tian Lian Huang, Joji Kusuyama, Sean D. Kodani, Justin Darcy, Gerson Profeta, Nayara Pereira, Rudolph E. Tanzi, Can Zhang, Thomas Serwold, Efi Kokkotou, Laurie J. Goodyear, Aaron M. Cypess, Luiz Osorio Leiria, Matthew Spite, Yu-Hua Tseng
Summary: The study found that cold exposure can improve obesity-induced inflammation and insulin resistance, and enhance glucose tolerance. The beneficial effects are dependent on brown adipose tissue (BAT) and liver. The researchers discovered that cold and beta 3-adrenergic stimulation promote the production of MaR2 from BAT, which reduces inflammation in obesity, particularly targeting macrophages in the liver.