Article
Plant Sciences
Justyna Stefanowicz-Hajduk, Magdalena Gucwa, Barbara Moniuszko-Szajwaj, Anna Stochmal, Anna Kawiak, J. Renata Ochocka
Summary: The bufadienolide compound bersaldegenin-1,3,5-orthoacetate exhibits strong cytotoxicity against cervical cancer cells by inducing cell cycle arrest and caspase-independent cell death through double-stranded DNA damage and overproduction of reactive oxygen species. The compound also leads to the overexpression of NF-Kappa-B inhibitor genes in the treated cells. These findings provide important insights for further research on cell death signaling pathways induced by bufadienolides.
PHARMACEUTICAL BIOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Fabien Granier, Sebastien Marie, Zahra Al Amir Dache, Julien Aityaya, Thibault Mazard, Laurent Garrelly, Corinne Prevostel
Summary: This study provides an in vitro methodology to assess the cytotoxicity and cell penetration of dendrigrafts of poly-L-lysine (DGLs) for potential use as nanoscale drug carriers for cancer treatment. The data suggests that DGLs exhibit cytotoxicity comparable to other dendrimers, are taken up by cancer cells quickly, and serum components can reduce their cytotoxicity.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Kavitha Alapati, Savithri S. Handanahal
Summary: Cholesterol oxidase demonstrates potent anticancer effects by affecting cell morphology and apoptotic protein expression, leading to a decrease in cell viability. Experiments show that this enzyme can exert cytotoxicity against human cancer cell lines at very low concentrations.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Zizheng Shen, Hansen Zhao, Huan Yao, Xingyu Pan, Jinlei Yang, Sichun Zhang, Guojun Han, Xinrong Zhang
Summary: This study used label-free mass cytometry to analyze the metabolites in human hepatocellular carcinoma cells at the single-cell level after interaction with NK cells. Several metabolites and metabolic pathways were identified to be involved in cell apoptosis, providing new insights into the heterogeneity of cell apoptosis and evaluating the cytotoxicity of NK cells.
Article
Immunology
Cheng Lin, Yvonne Marquardt, Stefan Ruetten, Liangliang Liao, Khosrow Rahimi, Tamas Haraszti, Jens Malte Baron, Matthias Bartneck
Summary: Macrophages play a critical role in the persistence of tattoo ink in human skin. However, there is a need for a comparison to other immune cells and a detailed analysis of REACH-compliant tattoo ink.
Article
Chemistry, Multidisciplinary
Fatemeh Mirzaee Rad, Farzaneh Tafvizi, Hassan Noorbazargan, Alireza Iranbakhsh
Summary: Artemisia turcomanica extract was used to synthesize Ag, ZnO, and Ag-doped ZnO nanoparticles. The anticancer activity of these nanoparticles against AGS gastric cancer cells was evaluated. The Ag-doped ZnO nanoparticles showed significant anticancer activity and induced apoptosis and cell cycle arrest. Rating: 8 out of 10.
JOURNAL OF NANOSTRUCTURE IN CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yiyang Li, Boqian Wang, Zara Ahmad Khan, Jie He, Edwin Cheung, Wenqiong Su, Aiting Wang, Hui Jiang, Lai Jiang, Xianting Ding
Summary: This study introduces a method of mass cytometry profiling for analyzing samples with limited or rare cell subsets using a platinum-chimeric carrier cell strategy. The results demonstrate the effectiveness of this method in preserving rare target cells and performing comprehensive single-cell subtype classification analysis.
ANALYTICAL CHEMISTRY
(2023)
Article
Immunology
Jasper Norenberg, Pal Jakso, Aliz Barakonyi
Summary: The study showed that during pregnancy, the proportion of CD56+ gamma delta T cells increases while maintaining potential cytotoxicity, although the cytotoxic strength is reduced, and PD-1 expression increases. Highly cytotoxic CD56+ gamma delta T cells tend to express PD-1, which may allow the inhibition of these cells after binding its ligand in the placenta.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tomas Malina, Katerina Polakova, Cordula Hirsch, Ladislav Svoboda, Radek Zboril
Summary: The application scope of carbon nanomaterials in various fields is expanding significantly, underscoring the importance of analyzing their potential harmful effects in vitro. Different types of carbon nanomaterials can cause various interferences in commonly used assays, highlighting the need for specific strategies to overcome such obstacles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Margarita Khariton, Conor J. McClune, Kara K. Brower, Sandy Klemm, Elizabeth S. Sattely, Polly M. Fordyce, Bo Wang
Summary: Microfluidic droplet assays enable single-cell PCR and sequencing analyses at unprecedented scales. Encapsulating cells within picoliter double emulsion allows sorting droplets with commercially available FACS machines, making it possible to isolate single cells based on phenotypes of interest. However, sorting DE droplets with standard cytometers requires small droplets that can pass FACS nozzles. Therefore, this study overcame limitations by using a plate-based RT-PCR assay to ameliorate the inhibition and demonstrated one-step RT-PCR from single cells in 20 pL DE droplets with fluorescence quantifiable via FACS.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Margarita Khariton, Conor J. McClune, Kara K. Brower, Sandy Klemm, Elizabeth S. Sattely, Polly M. Fordyce, Bo Wang
Summary: Microfluidic droplet assays enable single-cell PCR and sequencing analyses at unprecedented scales. However, sorting small droplets for FACS poses challenges for molecular biology. To overcome this, we used a plate-based RT-PCR assay and showed that heat lysis can alleviate inhibition for most cell lines, allowing RT-PCR to proceed in low-picoliter volumes.
ANALYTICAL CHEMISTRY
(2023)
Article
Immunology
Rosa G. M. Lammerts, Jacob van den Born, Magdalena Huberts-Kregel, Antonio W. Gomes-Neto, Mohammed R. Daha, Bouke G. Hepkema, Jan-Stephan Sanders, Robert A. Pol, Arjan Diepstra, Stefan P. Berger
Summary: The role of endothelial-specific antigens in allograft rejection has gained attention in recent years. In this study, a case of hyperacute rejection in a kidney transplant patient without pretransplant HLA-specific antibodies was reported. Crossmatch assays with primary renal endothelial cells showed reactivity with the patient's serum, indicating the presence of non-HLA endothelial cell-specific antibodies. Successfully removing these antibodies using rituximab and plasmapheresis led to a successful second kidney transplant for the patient.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Angela-Patricia Hernandez, Paula Diez, Pablo A. Garcia, Martin Perez-Andres, Anzhela Veselinova, Pablo G. Jambrina, Arturo San Feliciano, David Diez, Manuel Fuentes, Ma Angeles Castro
Summary: New lignohydroquinone conjugates (L-HQs) were synthesized using the hybridization strategy and showed dual cytotoxic effect against colorectal cancer cells. These conjugates were obtained from natural product podophyllotoxin and semisynthetic terpenylnaphthohydroquinones. The results demonstrate the validity of the hybridization strategy and suggest further research on non-lactonic cyclolignans.
Article
Chemistry, Multidisciplinary
Saghi Sepehri, Yasin Panahi, Daniyal Abbasi, Mahsa Jafari, Vahed Adhami, Ghazaleh Farhadi
Summary: This study successfully synthesized novel compounds based on the tetrahydropyrimidine scaffold, which showed good anti-cancer activity. Specifically, compound 4c exhibited the highest cytotoxicity and apoptosis induction, while compound 4i showed higher stimulation in the expression of related genes. Molecular docking and MD simulations suggested that the anti-cancer activity of these compounds may be achieved through inhibition of the Eg5 enzyme.
ARABIAN JOURNAL OF CHEMISTRY
(2024)
Article
Chemistry, Multidisciplinary
Hector Garcia Romeu, Sarah Deville, Anna Salvati
Summary: This study introduces a flow cytometry-based method to easily detect and characterize the uptake and trafficking of nanoparticles within cells, providing spatial and temporal resolution data. The method enables high-throughput analysis of nanoparticle uptake and intracellular trafficking, allowing for the determination of how nanoparticle design influences their behavior within cells.