4.7 Article

The structure of anammox granular sludge under varying long-term organic matter stress: Performance, physiochemical and microbial community

Journal

JOURNAL OF CLEANER PRODUCTION
Volume 323, Issue -, Pages -

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.jclepro.2021.129117

Keywords

Anammox granular sludge; Physiochemical characteristics; Microbial community; Nitrogen performance; Organic matter stress

Funding

  1. China Postdoctoral Science Foundation [2020M671400]
  2. National Natural Science Foundation of China [31760157]
  3. Natural Science Foundation of Jiangsu Province [BK20201450]
  4. Jiangsu Qing Lan Project
  5. Suzhou Science and Technology Planning Project [SS202016, ss2019022]
  6. Opening Fund of Jiangsu Provincial Key Laboratory of Environmental Science and Engineering [Zd1804]

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This study evaluated the long-term stress effects of varying concentrations of organic matter on nitrogen removal, physiochemical characteristics, and microbial community of anammox granular sludge in UASB reactors. The results showed that high COD concentrations caused disintegration of anammox granular sludge and changes in microbial community structure.
Long-term stress effects of varying concentrations of organic matter on nitrogen removal, physiochemical characteristics and microbial community of anammox granular sludge were evaluated in UASB (Up-flow Anaerobic Sludge Bed/Blanket) reactors. With increase of COD (Chemical Oxygen Demand) concentration (made by glucose) from 0 mg L-1 to 200 mg L-1, ammonium removal efficiency decreased sequentially from 97.71% to 46.52%, while the nitrite removal efficiency was maintained at 96.78-98.62%. The TN removal efficiency reached the highest (98.00%) at 50 mg L-1 of COD. However, 150 and 200 mg L-1 of COD caused the disintegration of anammox granular sludge, while also reducing extracellular polymeric substances (EPS) content, mechanical strength and sedimentation performance, respectively. The main bacteria at the phylum level had shifted from Chloroflexi to Proteobacteria, according to high-throughput Miseq sequencing analyses. Candidatus Brocadia became the dominant anammox genus under long-term organic matter stress, which correlated positively with ammonium and TN removal efficiency, instability coefficient (IC), protein/polysaccharide (PN/PS) and particle size, but negatively with COD concentration and EPS, respectively.

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