A novel method for the chaperone aided and efficient production of human proinsulin in the prokaryotic system

Increased C-Peptide Immunoreactivity in Insulin Autoimmune Syndrome (Hirata Disease) Due to High Molecular Weight Proinsulin

(2021) Richard G Kay et al.   CLINICAL CHEMISTRY

The congenital cataract‐causing mutations P20R and A171T are associated with important changes in the amyloidogenic feature, structure and chaperone‐like activity of human αB‐crystallin

(2020) Maryam Ghahramani et al.   BIOPOLYMERS

Using Insulin to Treat Poorly Controlled Type 2 Diabetes in 2020

(2020) Irl B. Hirsch et al.   JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION

Addition of Sialic Acid to Insulin Confers Superior Physical Properties and Bioequivalence

(2020) Daniel E. K. Kabotso et al.   JOURNAL OF MEDICINAL CHEMISTRY

A novel and more efficient biosynthesis approach for human insulin production in Escherichia coli (E. coli)

(2020) Kamini Govender et al.   AMB Express

Peak Fitting Applied to Fourier Transform Infrared and Raman Spectroscopic Analysis of Proteins

(2020) Azin Sadat et al.   Applied Sciences-Basel

Biological behaviors of mutant proinsulin contribute to the phenotypic spectrum of diabetes associated with insulin gene mutations

(2020) Heting Wang et al.   MOLECULAR AND CELLULAR ENDOCRINOLOGY

Expression and purification of recombinant human insulin from E. coli 20 strain

(2019) Marcin Zieliński et al.   PROTEIN EXPRESSION AND PURIFICATION

Modulating insulin fibrillation using engineered B-chains with mutated C-termini

(2019) Mohsen Akbarian et al.   BIOPHYSICAL JOURNAL

Insulin and its single-chain analogue

(2019) Ruifeng Mao et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Structural and functional characterization of D109H and R69C mutant versions of human αB-crystallin: The biochemical pathomechanism underlying cataract and myopathy development

(2019) Maryam Ghahramani et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Human αB-crystallin as fusion protein and molecular chaperone increases the expression and folding efficiency of recombinant insulin

(2018) Mohsen Akbarian et al.   PLoS One

Insulin Receptor Isoforms in Physiology and Disease: An Updated View

(2017) Antonino Belfiore et al.   ENDOCRINE REVIEWS

Importance of the positively charged residue at position 54 to the chaperoning function, conformational stability and amyloidogenic nature of human αA-crystallin

(2017) Kazem Khoshaman et al.   JOURNAL OF BIOCHEMISTRY

Simultaneous determination of insulin, DesB30 insulin, proinsulin, and C-peptide in human plasma samples by liquid chromatography coupled to high resolution mass spectrometry

(2016) Andreas Thomas et al.   Forensic Toxicology

Improving the refolding efficiency for proinsulin aspart inclusion body with optimized buffer compositions

(2016) Ying Chen et al.   PROTEIN EXPRESSION AND PURIFICATION

Large-Scale Refolding and Enzyme Reaction of Human Preproinsulin for Production of Human Insulin

(2015) Chang-Kyu Kim et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Prevention of aberrant protein aggregation by anchoring the molecular chaperone αB-crystallin to the endoplasmic reticulum

(2014) Shinichiro Yamamoto et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Refolding and simultaneous purification of recombinant human proinsulin from inclusion bodies on protein-folding liquid-chromatography columns

(2014) Jie Yuan et al.   BIOMEDICAL CHROMATOGRAPHY

Decorin differentially modulates the activity of insulin receptor isoform A ligands

(2014) Alaide Morcavallo et al.   MATRIX BIOLOGY

Cell factories for insulin production

(2014) Nabih A Baeshen et al.   Microbial Cell Factories

Biotechnology and genetic engineering in the new drug development. Part I. DNA technology and recombinant proteins

(2014) Agnieszka Stryjewska et al.   Pharmacological Reports

To fuse or not to fuse: What is your purpose?

(2013) Mark R. Bell et al.   PROTEIN SCIENCE

Studies on the regioselectivity and kinetics of the action of trypsin on proinsulin and its derivatives using mass spectrometry

(2012) Qurra-tul-Ann Afza Gardner et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Proinsulin Binds with High Affinity the Insulin Receptor Isoform A and Predominantly Activates the Mitogenic Pathway

(2012) Roberta Malaguarnera et al.   ENDOCRINOLOGY

Expression and characterization of human proinsulin fused to thioredoxin in Escherichia coli

(2011) Aldana Trabucchi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Increased expression, folding and enzyme reaction rate of recombinant human insulin by selecting appropriate leader peptide

(2011) Cheol-Ki Min et al.   JOURNAL OF BIOTECHNOLOGY

N-terminal segment of proinsulin C-peptide active in insulin interaction/desaggregation

(2010) Charlotte Nerelius et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Solution Structure of Proinsulin

(2010) Yanwu Yang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Raman spectra of amino acids and their aqueous solutions

(2010) Guangyong Zhu et al.   SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

Effects of citraconylation on enzymatic modification of human proinsulin using trypsin and carboxypeptidase B

(2009) Young-Jin Son et al.   BIOTECHNOLOGY PROGRESS

Study on preparation and unique properties of a novel insulin analogue with N-terminal Arg-4, Pro-3, Lys-2, Pro-1extension at insulin B-chain

(2009) Lu Yong et al.   REGULATORY PEPTIDES

Optimization of the industrial production of the recombinant precursor of human insulin

(2009) V. D. Gusarova et al.   RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY